Genes and Environment最新文献

{"title":"Potential for Computational Genotoxicity: A Report on Symposium 3 of the 53rd Annual Meeting of the Japanese Environmental Mutagen and Genome Society (JEMS), 2024.","authors":"Naoki Koyama, Ayako Furuhama, Naomi L Kruhlak, Nicolas Ken Shinada, Kazuki Izawa","doi":"10.1186/s41021-026-00358-y","DOIUrl":"10.1186/s41021-026-00358-y","url":null,"abstract":"<p><p>Symposium 3 of the 53rd Annual Meeting of the Japanese Environmental Mutagen and Genome Society (JEMS), entitled \"Potential for Computational Genotoxicity,\" was held at Shujitsu University, Okayama, Japan, on December 8, 2024. The symposium discussed the application of advanced informatics technologies, such as (quantitative) structure-activity relationship ((Q)SAR) and error-corrected next-generation sequencing (ecNGS), to the field of genotoxicity within the framework of computational genotoxicity. In this symposium, we invited three scientists who are global leaders in the field of computational genotoxicity. This report summarizes the key discussions and presentations from the symposium. The organizers hope this summary will increase awareness of computational genotoxicity.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":"48 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genome-wide interaction study of thyroid-stimulating hormone levels and particulate matter exposure among Koreans.","authors":"Young Jun Park, Hyun-Jin Kim, Ho-Young Son, Juhyun Kim, Jae Moon Yun, Hyuktae Kwon, Belong Cho, Jin-Ho Park, Jong-Il Kim","doi":"10.1186/s41021-026-00357-z","DOIUrl":"10.1186/s41021-026-00357-z","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo mammalian micronucleus test in mice confirms lack of genotoxic potential of a protein-rich powder derived from Xanthobacter sp. SoF1.","authors":"Bean Choi, John R Endres, Amy Clewell, Gábor Hirka, Erzsébet Béres","doi":"10.1186/s41021-026-00356-0","DOIUrl":"10.1186/s41021-026-00356-0","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13001180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ellagic acid is associated with reduced pancreatic carcinogenesis and modulation of the IL-6/STAT3 pathway.","authors":"Hiroyuki Kato, Aya Naiki-Ito, Masayuki Komura, Yuko Nagayasu, Motonori Sato, Xiaochen Kuang, Aya Nagano, Satoru Takahashi","doi":"10.1186/s41021-026-00353-3","DOIUrl":"10.1186/s41021-026-00353-3","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12964673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147343811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Development of a DNA damage assay system using stable human hepatocytes.","authors":"Masayuki Mishima, Kazuki Izawa, Masataka Tsuda, Yuichiro Higuchi, Shotaro Uehara, Hiroshi Suemizu, Kei-Ichi Sugiyama","doi":"10.1186/s41021-026-00355-1","DOIUrl":"10.1186/s41021-026-00355-1","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":"48 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12951896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147343798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of green tea extract to suppress colorectal polyp development in patients with familial adenomatous polyposis: a double-blind, randomized controlled trial Japan Familial Adenomatous Polyposis Prevention Study (J-FAPP Study I).","authors":"Hideki Ishikawa, Michihiro Mutoh, Tetsuro Yamane, Keiji Wakabayashi, Keiji Hirata, Takeo Iwama, Tomiyo Nakamura, Naohiro Tomita, Yutaka Matsuyama","doi":"10.1186/s41021-026-00354-2","DOIUrl":"10.1186/s41021-026-00354-2","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147343839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of germline variants in human population chronically exposed to high level natural background radiation in Kerala coast.","authors":"Vinay Jain, Divyalakshmi Saini, Radhakrishnan Sabarinathan, Birajalaxmi Das","doi":"10.1186/s41021-026-00352-4","DOIUrl":"10.1186/s41021-026-00352-4","url":null,"abstract":"<p><strong>Background: </strong>Genetic effects due to long term exposure to low doses of ionizing radiation (LDIR) in humans are not well understood. Human population living in high level natural radiation areas (HLNRAs) of Kerala coast in India are continuously exposed to chronic LDIR emanating from monazite containing beach sand for many generations. The background radiation level in this area varies from < 1.0 to 45mGy/year. The people residing in HLNRAs sometimes receives background radiation dose which is approximately 10-40 times higher than the people living in adjacent normal level natural radiation areas (NLNRAs). This population provides a unique opportunity to identify, if present, a mutational signature due to chronic low-dose radiation exposure in humans. We have employed whole exome sequencing approach to determine germline mutational changes in the lymphocytes of healthy individuals from HLNRAs (mean background dose: 31.8 ± 5.4 mGy/year, mean age: 43.0 ± 5.9 years) and compared them with healthy individuals from NLNRAs (mean background dose: 0.9 ± 0.2 mGy/year, mean age: 43.0 ± 11.3 years).</p><p><strong>Results: </strong>Our results revealed that the overall number of single nucleotide variants (SNVs) and insertions/deletions (indels) were not significantly different in HLNRA (7744 SNVs, 880 indels) and NLNRA (7951 SNVs, 856 indels) groups. A similar number of protein affecting mutations (PAMs) were observed in HLNRA (1925) and NLNRA (2082) individuals. Interestingly, several unique SNVs were identified in both the groups. In HLNRA, unique SNVs were overrepresented in genes involved in important biological pathways such as DNA repair (EXO1, PARP2, DDB1, POLQ, LIG1), epigenetic modification (KDM5D, SETDB2, KMT2B, BRD8, SIRT1), cell cycle progression (CDK14, CCND1) etc. Furthermore, significant predominance of C > T transitions which were unique to HLNRA group was observed preferentially at CpG dinucleotide regions. Analysis with REVEL and AloFT tools did not show any increase in potentially pathogenic mutations including those involved in carcinogenesis in HLNRA individuals exposed to chronic radiation.</p><p><strong>Conclusion: </strong>This study did not show any significant changes in genetic variants due to long term exposure to LDIR in human population living in HLNRAs of Kerala coast. However, presence of unique SNVs and C > T transitions in CpG islands of HLNRA individuals indicate the possible role of epigenetic mechanisms i.e. DNA methylation in response to chronic LDIR in this population. This study significantly enhances the current understanding of radiation induced genetic changes and associated cancer risk in human population.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a DNA damage assay system using stable human hepatocytes.","authors":"Masayuki Mishima, Kazuki Izawa, Masataka Tsuda, Yuichiro Higuchi, Shotaro Uehara, Hiroshi Suemizu, Kei-Ichi Sugiyama","doi":"10.1186/s41021-025-00347-7","DOIUrl":"10.1186/s41021-025-00347-7","url":null,"abstract":"<p><strong>Background: </strong>Overcoming species differences in metabolism between humans and animals remains a critical challenge in toxicological studies. Rat liver S9 fraction has long been the gold standard for exogenous metabolic activation in in vitro genotoxicity tests. Experiences with human S9 or human primary hepatocytes have suggested that the human materials are unsuitable for standardized testing due to high variability. Nevertheless, there is growing interest in genotoxicity evaluation using metabolic systems that more closely mimic human physiology.</p><p><strong>Results: </strong>We developed an in-cell ELISA system to measure γH2AX as a DNA damage marker in stable human hepatocytes (γH2AX-SHE). HepaSH cells are consistently available human hepatocytes that stably express a range of metabolic enzymes and drug transporters in vitro. Due to their highly differentiated and non-proliferative nature, conventional genotoxicity endpoints such as micronuclei formation, chromosomal aberrations, or mutant colony assays are not applicable. We used γH2AX, a sensitive DNA damage marker, in this assay system. Indirect mutagens including benzo(a)pyrene, aristolochic acid, and 2-Amino-1-methyl-6-phenylimidazo(4,5-b)pyridine induced dose-dependent increases in γH2AX across all three HepaSH strains. Time-course analysis following benzo(a)pyrene exposure indicated that a treatment duration of 16 hours or longer was necessary to detect genotoxic responses. Prolonged exposure for 48 hours resulted in extensive cell death, which may interfere with γH2AX quantification.</p><p><strong>Conclusions: </strong>We demonstrated that γH2AX-SHE can serve as a valuable tool for detecting DNA damage under conditions that mimic human metabolic activity. Based on the findings in this study, we recommend the following assay conditions for γH2AX-SHE: a 24-hour treatment period, a DMSO concentration not exceeding 1%, and careful interpretation of positive responses observed at highly cytotoxic doses - defined as approximately less than 60% cell survival - as these may lack biological relevance.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":"48 1","pages":"3"},"PeriodicalIF":1.9,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12849352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146062518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CYP1B1- and CYP1A1-Template systems and their application to metabolism and inhibition.","authors":"Yasushi Yamazoe, Kaori Ambe, Masahiro Tohkin, Takashi Yamada, Kenichi Masumura","doi":"10.1186/s41021-025-00351-x","DOIUrl":"10.1186/s41021-025-00351-x","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"1"},"PeriodicalIF":1.9,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for the assessment and control of mutagenic impurities in pharmaceuticals.","authors":"Masamitsu Honma","doi":"10.1186/s41021-025-00349-5","DOIUrl":"10.1186/s41021-025-00349-5","url":null,"abstract":"","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"26"},"PeriodicalIF":1.9,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12723853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}