Gene Reports最新文献

{"title":"LncRNAs PVT1, HULC, and HOTTIP: A promising biomarker trio for diffuse large B-cell lymphoma","authors":"Milad Shahsavari ,&nbsp;Sedigheh Arbabian ,&nbsp;Farzaneh Hosseini ,&nbsp;Mohamad Reza Razavi","doi":"10.1016/j.genrep.2025.102182","DOIUrl":"10.1016/j.genrep.2025.102182","url":null,"abstract":"<div><h3>Background</h3><div>Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma (NHL) and is characterized by heterogeneity in morphology, genetics, and behavior. While the standard immunochemotherapy regimen, R-CHOP, can lead to sustained complete remission in most patients, those with relapses and poor prognoses require alternative R2-CHOP therapy.</div></div><div><h3>Methods</h3><div>Experimental and bioinformatic approaches explored the signaling pathways of three critical lncRNAs, PVT1, HULC, and HOTTIP, and their biological functions. The expression of these lncRNAs was quantitatively evaluated using real-time PCR in 100 patients before and after conventional treatment.</div></div><div><h3>Results</h3><div>PVT1, HULC, and HOTTIP expression were significantly elevated by 7.412 ± 2.497, 6.42 ± 3.32, and 4.09 ± 2.38 folds, respectively, compared to normal cases (<em>p</em> &lt; 0.001). The expression levels were significantly higher in DLBCL patients aged &gt;60 than those aged &lt;60. A significant positive correlation was observed between HULC and HOTTIP expression. Post-treatment measurements showed a significant downregulation of PVT1 and HOTTIP (p &lt; 0.001 and <em>p</em> = 0.032, respectively). Overexpression of lncRNA-miRNA interaction network analysis indicated deregulated targets, including hsa-miR-200a-3p, hsa-miR-372-3p, hsa-miR-186-5p, and others.</div></div><div><h3>Conclusions</h3><div>The long non-coding RNAs (lncRNAs) PVT1, HULC, and HOTTIP, which exhibited elevated expression levels in patients with diffuse large B-cell lymphoma (DLBCL) before treatment and decreased to normal levels following treatment, could serve as valuable diagnostic biomarkers or prognostic indicators of treatment response. Their distinct conditional expression patterns across different age demographics further support their potential utility.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102182"},"PeriodicalIF":1.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HLA Class I and II genes: A key factor for type one diabetes susceptibility","authors":"Gaurang Telang ,&nbsp;Smriti Mishra ,&nbsp;Anurag Sureshbabu ,&nbsp;Sameer Chiloo ,&nbsp;Shantanu Joshi ,&nbsp;Senthil Thyagarajan","doi":"10.1016/j.genrep.2025.102183","DOIUrl":"10.1016/j.genrep.2025.102183","url":null,"abstract":"<div><div>Type 1 diabetes (T1D) is one of the most chronic autoimmune diseases categorized by pancreatic β-cell destruction. The susceptibility towards T1D is determined using a composite interface between numerous genetic and environmental factors. The clinical inception of T1D is led by the presence of autoantibodies in contrast to β-cells. The main genetic mechanism of T1D is associated with the Major histocompatibility complex (MHC) i.e., HLA genes and it has about 50 % inclination of genetic influence located on the short arm of chromosome 6p21 extends around 4000 kb, and holds over 200 genes. These are the key fragments of genetic risk factors of T1D. The genes encrypt HLA Class I and II which mediate the pathogenetically immune mechanism. Their main purpose is intrusion and inflammation in the pancreatic islets known as insulitis. The utmost links between disease and HLA loci are with Class II genes expressed in antigenic presenting cells and also play a great role in β-cell autoimmunity, tolerance, and autoreactive T-cell response. Other non-HLA genes are also involved in this development. T cells play an important role in the recognition of islet autoantigens along with the cytokines. Many in-vivo models also provide the genetic analysis that shows the presence of frequent chromosomal regions susceptible to T1D development. Recent advances in techniques like HLA genotyping, DNA typing, and next-generation sequencing expand the genetic element information with distinct prominence of therapeutic as well as treatment, and diagnosis approaches.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102183"},"PeriodicalIF":1.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of PCSK9 gene polymorphism on atorvastatin efficacy in Iraqi hyperlipidemic patients","authors":"Khalid Qasim Mohammed ,&nbsp;Mazin H. Ouda ,&nbsp;Suzanne Jubair","doi":"10.1016/j.genrep.2025.102181","DOIUrl":"10.1016/j.genrep.2025.102181","url":null,"abstract":"<div><h3>Background</h3><div>Many clinical observations within the local hyperlipidemic population indicate that many individuals continue to experience elevated cholesterol and low-density lipoprotein (LDL) levels despite being treated with atorvastatin. Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a crucial role in regulating LDL metabolism. This study investigates the impact of the rs28942111; T &gt; A single nucleotide polymorphism (SNP) in PCSK9 gene on the effectiveness of atorvastatin therapy among hyperlipidemic patients in Iraq.</div></div><div><h3>Methodology</h3><div>This cross-sectional study involved 149 Iraqi patients, male and female aged 28 to 85 years, who were diagnosed with primary hyperlipidemia and had been treated with oral atorvastatin (40 mg) for a minimum of six months. The lipid profiles and liver function were evaluated, the genetic analysis to identify the rs28942111 SNP was performed using the allele-specific polymerase chain reaction technique.</div></div><div><h3>Results</h3><div>The distribution of genotypes for the rs28942111; T &gt; A SNP revealed that 128 patients (85.9 %) were TT carriers, while 21 patients (14.1 %) were AA carriers, no heterozygous mutant type was detected. Significant high levels of LDL, total cholesterol (TC), and aspartate transaminase were observed among the AA carriers compared to TT carriers (<em>p</em>-value equals to 0.001).</div></div><div><h3>Conclusion</h3><div>The rs28942111 SNP in the PCSK9 gene is significantly associated with higher LDL and TC levels, this suggests that this SNP might play an important role in the therapeutic response to atorvastatin among hyperlipidemic patients in Iraq.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102181"},"PeriodicalIF":1.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of single-nucleotide polymorphisms in opioid receptors genes on opioid use disorder susceptibility among Egyptian population: A case-control study","authors":"Amira A. Abdelnoor ,&nbsp;Mostafa M. Kamel ,&nbsp;Fatma M. Elgazzar ,&nbsp;Afaf M. Elsaid ,&nbsp;Anas M. AboSamak","doi":"10.1016/j.genrep.2025.102180","DOIUrl":"10.1016/j.genrep.2025.102180","url":null,"abstract":"<div><h3>Background</h3><div>Opioid use disorder (OUD) vulnerability, progression, and course are driven by biological, developmental, environmental, and genetic factors. Single nucleotide polymorphisms (SNPs) in opioid receptor (OPR) genes can influence receptor expression, structure, or function, potentially altering OUD susceptibility and impacting treatment response and relapse rates. Delta receptors 1 (OPRD1), kappa receptors 1 (OPRK1), and mu receptors 1 (OPRM1) are commonly studied OUD-related genes. They were examined in different ethnic groups, and their results conflicted. Therefore, this research aimed to determine the impact of sociodemographic and genetic factors on OUD risk in a sample of Egyptians. It fills a crucial gap in understanding the effect of SNPs within OPR genes on OUD among Egyptians.</div></div><div><h3>Methods</h3><div>This case-control study evaluated 50 opioid substance users versus 50 healthy, age- and sex-matched non-substance users. The sociodemographic profiles and opioid use data were collected from medical records and semi-structured interviews. Participants were assessed through the DSM-5 and ICD-11 Symptom Checklist. The SNPs in T921C of OPRD1, G36T of OPRK1, and A118G of OPRM1 genes were adopted. Venous blood samples were collected for DNA extraction and gene SNPs were examined after PCR amplification under an ultraviolet transilluminator.</div></div><div><h3>Results</h3><div>The OUD group exhibited polymorphisms in OPRD1 (2 %), OPRK1 (10 %), and OPRM1 (2 %) with no significant associations between SNPs and OUD. Multivariable regression analysis identified important OUD risk factors, including low education levels and a positive family history of SU. They were associated with an increased likelihood of OUD with 8 and 6 times, respectively.</div></div><div><h3>Conclusion</h3><div>This study provides initial evidence suggesting that OUD susceptibility among Egyptians is mainly related to sociodemographic factors rather than genetic polymorphisms in OPR genes. Pre-university education, including illiterate participants, as well as participants with primary and secondary education, increased OUD susceptibility risk &gt;8 times (<em>P</em> value &lt; 0.001, adjusted odds ratio 8.652 with 95 % confidence interval). A positive family history of SU was linked with increased susceptibility by more than sixfold (<em>P</em> value 0.029, adjusted odds ratio 6.101 with 95 % confidence interval).</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102180"},"PeriodicalIF":1.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Independent and interactive effect of trace elements imbalance and TNF-α promoter variants as risk modifiers in pediatric asthma","authors":"Shivani Singh ,&nbsp;Anumesh K. Pathak ,&nbsp;Manish Raj Kulshrestha ,&nbsp;Aditi Singh ,&nbsp;Vandana Tiwari ,&nbsp;Shetanshu Srivastava","doi":"10.1016/j.genrep.2025.102179","DOIUrl":"10.1016/j.genrep.2025.102179","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the association between trace element (TEs) levels (zinc, copper, iron, selenium, and magnesium) and <em>TNF-α-308</em> G/A polymorphism and childhood asthma risk, severity, inflammatory markers (hs-CRP, TNF-α), and cell counts (neutrophils and eosinophils) in an Indian cohort.</div></div><div><h3>Methods</h3><div>This prospective case-control study included 433 children (230 patients with asthma and 203 controls). Serum TEs levels were quantified using inductively coupled plasma mass spectrometry, <em>TNF-α-308</em> G/A polymorphism was analyzed via restriction fragment length polymorphism, and serum TNF-α levels were measured using ELISA. Logistic regression models (adjusted for age, sex, and family history) and correlation analyses were conducted.</div></div><div><h3>Results</h3><div>Asthmatic children had significantly higher copper levels (1650.40 vs. 1274.30 μg/L;<em>p</em> = 0.002) and lower zinc (464.16 vs. 632.43 μg/L) and iron (36.00 vs. 60.00 μg/L; <em>p</em> &lt; 0.0001) levels. The <em>TNF-α-308</em> GG genotype significantly increased the asthma risk by 3.2-fold, and the GA genotype conferred a 2.5-fold increased risk. Low zinc levels combined with variant genotypes (GA + GG) increased the risk by 1.9-fold, while low iron levels with these genotypes amplified the risk by 3.2-fold. High Cu levels with variant genotypes increased the risk by 2.5-fold. Zinc was negatively correlated with TNF-α (<em>r</em> = −0.23), hs-CRP (<em>r</em> = −0.33), and neutrophil counts (<em>r</em> = −0.64), whereas copper was positively correlated with TNF-α (<em>r</em> = 0.36; <em>p</em> &lt; 0.0001) and neutrophil counts (<em>r</em> = 0.68). Elevated hs-CRP and TNF-α levels are associated with reduced lung function (FEV1) and increased asthma severity.</div></div><div><h3>Conclusion</h3><div>The interplay between TEs imbalances, TNF-α polymorphisms, and inflammatory responses significantly influences the risk and severity of childhood asthma.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102179"},"PeriodicalIF":1.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HR9 cell-penetrating peptide: An effective delivery approach of gene editing vectors for tumor treatment","authors":"Ali Anvar ,&nbsp;Azam Bolhassani ,&nbsp;Iman Salahshoorifar ,&nbsp;Shiva Irani","doi":"10.1016/j.genrep.2025.102177","DOIUrl":"10.1016/j.genrep.2025.102177","url":null,"abstract":"<div><div>HR9 cell-penetrating peptide is an arginine-rich peptide that can deliver various molecules into the cells. In this study, we assessed the efficacy of HR9 peptide in transporting the modified CRISPR vectors pSpCas9n (BB)-2A-GFP (PX461) and pSpCas9n (BB) (PX460) into HPV16 C3 tumor cells, and explored the impact of their treatment through tumor growth assay, and immunohistochemistry and staining techniques. Herein, the HPV16 E6 and E7 genes were targeted using CRISPR/Cas9 technology. Our findings showed improved transfection rates of HR9 peptide compared to the Lipofectamine 2000 transfection reagent for delivery of the CRISPR/Cas9 vectors into C3 tumor cells. Mice treated with PX460-E7 and PX460-E6 + PX460-E7 showed important therapeutic effects regarding tumor volume reduction and pathological analysis. Our study demonstrated the potent delivery of HR9/CRISPR/Cas9 nanoparticles into C3 tumor cells <em>in vitro</em> and <em>in vivo</em>, and also the high efficiency and specificity of CRISPR/Cas9-mediated genome editing in mice.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102177"},"PeriodicalIF":1.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HAVCR1 in cancer: A systematic review of its dual-faceted role as a biomarker and therapeutic target","authors":"Amirali Soltaninegar ,&nbsp;Fatemeh Sadat Jalilzadeh Ghahi ,&nbsp;Sepideh Hosseini ,&nbsp;Najaf Allahyari Fard","doi":"10.1016/j.genrep.2025.102178","DOIUrl":"10.1016/j.genrep.2025.102178","url":null,"abstract":"<div><div>Hepatitis A virus cellular receptor 1 (HAVCR1), also known as KIM-1 in kidney diseases and TIM-1 or CD365 in immunology, is a class 1 integral membrane glycoprotein member that plays a pivotal role in cancer progression, diagnosis, and potential treatment. This review study aims to explore the various aspects of the HAVCR1 protein, focusing on its role as a potential biomarker and therapeutic target in different types of cancers and analyze the correlation of HAVCR1 with prognosis, underlying molecular mechanisms, and its clinical potential in cancer diagnosis and treatment. In cancers such as RCC, ESCA, LIHC, PAAD, LUAD, and OV, HAVCR1 is associated with poor prognosis, whereas in others like COAD, RB, SKCM, and BLCA, it correlates with improved outcomes. HAVCR1 contributes to cancer progression through the MAPK/ERK and PI3K/AKT pathways, which regulate immune suppression, cell growth, survival, migration, and angiogenesis. Its expression level, shedding, and localization significantly influence its role in cancer in the plasma or internal membranes. The release of the HAVCR1 ectodomain during shedding and its detectability in urine, blood, and cerebrospinal fluid highlight its potential as a biomarker for diagnosing specific cancers. Furthermore, HAVCR1 represents a promising target for therapeutic interventions in oncology.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102178"},"PeriodicalIF":1.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of recombinant canine perilipin3 (PLIN3) and its assessment for immunoreactivity","authors":"Amrita Behera ,&nbsp;Ghanshyam Sahu ,&nbsp;Vineet Kumar Pandey ,&nbsp;Franco PS ,&nbsp;Pawan Kumar ,&nbsp;Mukesh Kumar ,&nbsp;Mohini Saini ,&nbsp;Karuna Irungbam","doi":"10.1016/j.genrep.2025.102157","DOIUrl":"10.1016/j.genrep.2025.102157","url":null,"abstract":"<div><div>Perilipins are the lipid droplet associated proteins (LDAPs) involved in the biogenesis and stabilization of lipid droplets. LDAPs contribute significantly to the pathogenesis of several diseases stemming from dysregulated lipid metabolism including cancers. Several studies indicate lipid droplet (LD) accumulation in cancer cells, emphasizing the critical importance of comprehending the role of perilipins in cancers. This study seeks to generate hyperimmune sera directed against canine perilipin3 (PLIN3), with the aim of investigating the expression of perilipin3 in canine mammary tumors (CMT). The limited availability of canine specific commercial perilipin antibodies substantiate the necessity of developing canine specific antibodies to better understand the role of perilipins in CMT pathogenesis. A fragment of the canine perilipin3 gene was amplified effectively from total RNA extracted from canine mammary tumor samples and it was cloned and expressed as a recombinant protein in pET32a+ prokaryotic system. The recombinant canine PLIN3 protein was purified using Ni- NTA (Nickel-nitroacetic acid) affinity chromatography and used to generate hyperimmune sera in chickens. The generated antisera showed strong reactivity to the recombinant canine PLIN3 protein in dot blot assays. Immunohistochemistry analysis revealed specific immunoreactivity to PLIN3 in CMT tissue samples, with elevated expression levels compared to control tissues. These data indicate that PLIN3 may be differentially expressed in canine mammary tumors, highlighting its potential role in tumor progression. The study provides a valuable tool for advancing the investigation of lipid droplet-associated proteins in canine cancers, particularly CMT, and offers insights into lipid metabolism as a possible therapeutic target in veterinary oncology.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102157"},"PeriodicalIF":1.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of diabetes and hypertension: A performance comparison between transcriptome data and clinical data","authors":"Pratheeba Jeyananthan,&nbsp;T.A.P. Dharmasena,&nbsp;W.D.A. Nuwansiri","doi":"10.1016/j.genrep.2025.102176","DOIUrl":"10.1016/j.genrep.2025.102176","url":null,"abstract":"<div><div>Diabetes and hypertension are two related diseases common among most of the people all over the word. The impact of these diseases can elevate the risk of developing additional health issues, including cardiovascular disease, kidney disease, and other related conditions. Numerous research initiatives are underway to uncover the underlying mechanisms of these diseases. This study compares the roles of clinical data and transcriptome data in the diagnosis of patients with diabetes or hypertension. This study utilizes two distinct datasets, each containing unique clinical features along with a third dataset that includes transcriptome characteristics, all analyzed through machine learning algorithms. In both diseases, there is a marked difference in the accuracies of the models based on various clinical features. This highlights the importance of selecting appropriate clinical features to develop an optimal diagnostic model for these conditions. In comparing the best clinical model with the transcriptome model for diagnosing diabetic patients, it has been found that the transcriptome data yields superior results. Conversely, for diagnosing hypertension patients, the clinical data proves to be more effective. Hence, identifying the appropriate set of clinical features, clinical data could become more effective for diagnosing both diabetes and hypertension.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102176"},"PeriodicalIF":1.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of DREB transcription factors in Medicago truncatula: Insights into their roles in development and abiotic stress response","authors":"Loua Haddoudi ,&nbsp;Mariem Ayadi ,&nbsp;Sabrine Hdira ,&nbsp;Mohsen Hanana ,&nbsp;Irene Romero ,&nbsp;Hatem Ben Jouira ,&nbsp;Naceur Djébali ,&nbsp;Ludidi Ndiko ,&nbsp;Maria Teresa Sanchez Ballesta ,&nbsp;Chedly Abdelly ,&nbsp;Mounawer Badri","doi":"10.1016/j.genrep.2025.102170","DOIUrl":"10.1016/j.genrep.2025.102170","url":null,"abstract":"<div><div>Dehydration-responsive-element binding (<em>DREB</em>) proteins play a crucial role in drought, salt, and environmental stress tolerance. In this study, we identified and annotated fifty-four <em>DREB</em> genes from the <em>Medicago truncatula</em> genome. These genes were analyzed at the molecular level, focusing on gene classification, genomic organization, phylogeny, synteny, structural features, and expression profiles. Phylogenetic analysis revealed that <em>MtDREB</em> proteins are categorized into six subgroups (A1–A6), with highly conserved motif compositions among them. Expression profiling showed that <em>MtDREB</em> genes are differentially expressed in various plant organs and under abiotic stresses (cold, salinity, and dehydration), with 30 % exhibiting high expression during flowering and development. Data from RNA-seq and microarrays demonstrated that 76 % of <em>MtDREB</em> genes are differentially expressed under at least one stress condition, indicating their involvement in various signaling pathways activated by abiotic stresses. Notably, <em>MtDREB05</em>, primarily induced under osmotic stress, appears to be a promising candidate for improving abiotic stress tolerance. These findings will enhance our understanding of the <em>DREB</em> family and aid in functional validation of <em>DREB</em>genes in <em>M. truncatula</em> and related forage species.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102170"},"PeriodicalIF":1.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}