Molecular characterization of Entamoeba complex species in clinical samples from Eastern of Iran in 2024

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-21 DOI:10.1016/j.genrep.2025.102203

Mahmoodreza Behravan , Fetameh Alipour , Mostafa Ashrafi , Narges Ekrahi , Abdol Sattar Pagheh , Amir Tavakoli Kareshk

{"title":"Molecular characterization of Entamoeba complex species in clinical samples from Eastern of Iran in 2024","authors":"Mahmoodreza Behravan , Fetameh Alipour , Mostafa Ashrafi , Narges Ekrahi , Abdol Sattar Pagheh , Amir Tavakoli Kareshk","doi":"10.1016/j.genrep.2025.102203","DOIUrl":null,"url":null,"abstract":"<div><div>Amoebiasis, an infection caused by the protozoan parasite Entamoeba histolytica, is a highly significant disease that can lead to numerous complications and mortality in humans. While E. histolytica, Entamoeba dispar, and Entamoeba moshkovskii share morphological similarities, they differ significantly in their pathogenicity. Recognizing the significance of determining the prevalence of different Entamoeba species, this study aimed to molecularly identify the various species in patients referred to the Imam Reza Hospital laboratory in Birjand during the years 2023–2024.In this cross-sectional study, a total of 350 stool samples from patients referred to the Imam Reza Hospital laboratory in Birjand were collected through non-random convenience sampling during the years 2023–2024. The samples were examined using both microscopic and molecular methods. The data obtained from the study were analyzed using SPSS (Statistical Package for the Social Sciences) software version 27.00.Microscopic examination revealed that out of the 350 samples, the prevalence of the Entamoeba complex was 35 (10 %), Blastocystis was 13 (7.3 %), and Giardia lamblia was 5 (1.42 %). Nested-PCR (Polymerase Chain Reaction) results showed that out of the 35 positive Entamoeba complex samples, 20 samples (57.1 %) were Entamoeba coli (highest frequency), 11 samples (31.5 %) were E. dispar, 2 samples (5.7 %) were E. histolytica, and 2 samples (5.7 %) were E. moshkovskii. In this study, significant relationship was observed between the relative frequency of Entamoeba complex with education level, location and occupation (Pvalue < 0.05), but no significant relationship was observed between the frequency of Entamoeba complex with age, gender, type of stool and symptom (Pvalue > 0.05). The findings of this study reveal the prevalence of the Entamoeba complex among patients referred to the Imam Reza Hospital laboratory in Birjand, highlighting the need for increased focus on disease epidemiology. These findings emphasize the need for additional research to gain a deeper understanding of the factors that affect the prevalence and distribution of the Entamoeba complex.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102203"},"PeriodicalIF":1.0000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425000767","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Amoebiasis, an infection caused by the protozoan parasite Entamoeba histolytica, is a highly significant disease that can lead to numerous complications and mortality in humans. While E. histolytica, Entamoeba dispar, and Entamoeba moshkovskii share morphological similarities, they differ significantly in their pathogenicity. Recognizing the significance of determining the prevalence of different Entamoeba species, this study aimed to molecularly identify the various species in patients referred to the Imam Reza Hospital laboratory in Birjand during the years 2023–2024.In this cross-sectional study, a total of 350 stool samples from patients referred to the Imam Reza Hospital laboratory in Birjand were collected through non-random convenience sampling during the years 2023–2024. The samples were examined using both microscopic and molecular methods. The data obtained from the study were analyzed using SPSS (Statistical Package for the Social Sciences) software version 27.00.Microscopic examination revealed that out of the 350 samples, the prevalence of the Entamoeba complex was 35 (10 %), Blastocystis was 13 (7.3 %), and Giardia lamblia was 5 (1.42 %). Nested-PCR (Polymerase Chain Reaction) results showed that out of the 35 positive Entamoeba complex samples, 20 samples (57.1 %) were Entamoeba coli (highest frequency), 11 samples (31.5 %) were E. dispar, 2 samples (5.7 %) were E. histolytica, and 2 samples (5.7 %) were E. moshkovskii. In this study, significant relationship was observed between the relative frequency of Entamoeba complex with education level, location and occupation (P_value < 0.05), but no significant relationship was observed between the frequency of Entamoeba complex with age, gender, type of stool and symptom (P_value > 0.05). The findings of this study reveal the prevalence of the Entamoeba complex among patients referred to the Imam Reza Hospital laboratory in Birjand, highlighting the need for increased focus on disease epidemiology. These findings emphasize the need for additional research to gain a deeper understanding of the factors that affect the prevalence and distribution of the Entamoeba complex.

查看原文本刊更多论文

2024年伊朗东部临床标本内阿米巴复合体的分子特征

阿米巴病是一种由原生动物寄生虫溶组织内阿米巴原虫引起的感染，是一种非常严重的疾病，可导致人类许多并发症和死亡。虽然溶组织内阿米巴、异速内阿米巴和莫什科夫斯基内阿米巴在形态上有相似性，但它们的致病性却有显著差异。认识到确定不同内阿米巴物种流行的重要性，本研究旨在从分子上鉴定2023-2024年期间在Birjand伊玛目礼萨医院实验室转诊的患者中的各种物种。在这项横断面研究中，通过非随机方便抽样，在2023-2024年间收集了来自Birjand伊玛目礼萨医院实验室的患者的350份粪便样本。用显微镜和分子方法对样品进行了检查。本研究数据采用SPSS (Statistical Package for The Social Sciences) 27.00版软件进行分析。镜检结果显示，350份标本中，内阿米巴复合体35种（10%），囊虫13种（7.3%），贾第鞭毛虫5种（1.42%）。巢式pcr （Polymerase Chain Reaction）结果显示，35份复合体阳性标本中，大肠内阿米巴阳性20份（57.1%），差异内阿米巴阳性11份（31.5%），溶组织内阿米巴阳性2份（5.7%），莫氏内阿米巴阳性2份（5.7%）。在本研究中，内阿米巴复合体的相对频率与受教育程度、地点和职业有显著相关(p值<；0.05)，但内阿米巴复发率与年龄、性别、大便类型及症状无显著相关性(p值>；0.05)。这项研究的结果揭示了在Birjand的Imam Reza医院实验室转诊的患者中，内阿米巴原虫复合体的流行情况，突出表明需要加强对疾病流行病学的关注。这些发现强调需要进行更多的研究，以便更深入地了解影响内阿米巴原虫流行和分布的因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.