Gene Reports最新文献

{"title":"Genomic insights and biotechnological potential of “Mesobacillus aurantius” strain S13: A canthaxanthin pigment-producing bacterium","authors":"Shankar Karthik ,&nbsp;Gurusamy Raman ,&nbsp;Arumugam Srinivasan ,&nbsp;Syed Gulam Dastager ,&nbsp;Vasudev Wagh ,&nbsp;Niraikulam Ayyadurai","doi":"10.1016/j.genrep.2025.102287","DOIUrl":"10.1016/j.genrep.2025.102287","url":null,"abstract":"<div><div>This study explores the discovery and characterization of pigments, from a microorganism thriving under environmentally challenging conditions. Utilizing a polyphasic taxonomic approach, we successfully isolated and identified a bacterial strain S13 within the <em>Mesobacillus</em> genus and Bacillaceae family, designated as “<em>Mesobacillus aurantius</em>” S13. We confirmed the species based on comprehensive phenotypic, physiological, and biochemical analyses, molecular phylogenetics, DNA–DNA hybridization, average nucleotide identity, and whole-genome sequencing. The strain S13 (MTCC13141 = VKM B-3583 = NBIMCC 9084) possesses a 4.81 Mbp genome that encodes genes for pigment production. Notably, this study uses various spectroscopic methods to characterize canthaxanthin, an orange pigment and delineates its biosynthetic pathway from the genome of S13. The methylerythritol phosphate (MEP) pathway and canthaxanthin biosynthesis genes highlight the capacity of the bacterium to synthesize crucial isoprenoids and terpenoids, including canthaxanthin. This research provides a detailed account of the isolation, characterization, and taxonomic classification of “<em>Mesobacillus aurantius</em>” S13 and underscores the potential biotechnological applications of the bacterium in producing pharmaceuticals, biofuels, and cosmetics. Therefore, “<em>Mesobacillus aurantius</em>” S13 is a potentially valuable microbial resource for the industrial production of isoprenoids and pigments.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102287"},"PeriodicalIF":1.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking lncRNA MEG3, RORγt, and IL-17 to immune dysregulation in Hashimoto's thyroiditis: Potential for early diagnosis and monitoring","authors":"Sara A. El Derbaly ,&nbsp;Eman Mansour ,&nbsp;E.A.E. Badr ,&nbsp;Moustafa Bakrey Hamed Ata ,&nbsp;Mohamed Farag Ali Assar","doi":"10.1016/j.genrep.2025.102293","DOIUrl":"10.1016/j.genrep.2025.102293","url":null,"abstract":"<div><h3>Background</h3><div>The study focused on Hashimoto's thyroiditis (HT), a chronic autoimmune thyroid disorder that causes inflammation and immune-mediated damage to the thyroid gland. Investigating biomarkers could improve the diagnosis and treatment of HT. This research was conducted to explore the correlation among lncRNA MEG3, RORγt, and IL-17 gene expressions and their possible utility as diagnostic and predictive markers in HT patients.</div></div><div><h3>Methods</h3><div>The research involved 150 participants split evenly between HT patients and healthy individuals. Various assessments were performed, including histories, physical exams, and lab tests. The transcript levels of lncRNA MEG3, RORγt, and IL-17 were quantified using real-time PCR following RNA extraction from collected blood samples.</div></div><div><h3>Results</h3><div>Higher levels of lncRNA MEG3, RORγt, and IL-17 gene expressions were indicated in HT patients in comparison with those in the control group. The study found significant positive correlations between lncRNA MEG3 &amp; RORγt, RORγt &amp; IL-17, as well as lncRNA MEG3 &amp; IL-17.</div></div><div><h3>Conclusion</h3><div>LncRNA MEG3 and IL-17 were identified as predictors of HT. Elevated the levels of these markers were related to the severity of the condition. LncRNA MEG3 contributes to the control of gene expression and immune activities, while IL-17 acts as a proinflammatory cytokine. They show potential as biomarkers for diagnosis and monitoring, but larger, long-term studies with protein-level validation are needed to confirm their clinical value.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102293"},"PeriodicalIF":1.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in rhizosphere microbial community structure in oilseed rape with different degrees of clubroot severity","authors":"Zhongmei Zhang ,&nbsp;Zijin Hu ,&nbsp;Xiaoqin Huang ,&nbsp;Yaoying Yu ,&nbsp;Yue Deng ,&nbsp;Pei Song ,&nbsp;Yong Liu ,&nbsp;Lei Zhang ,&nbsp;Xiaoxiang Yang","doi":"10.1016/j.genrep.2025.102294","DOIUrl":"10.1016/j.genrep.2025.102294","url":null,"abstract":"<div><div>To examine how clubroot severity impacts oilseed rape rhizosphere microbial communities, this study analyzed rhizosphere soil samples from healthy, mildly infected, and severely infected plants. We assessed total microorganism content and conducted 16S rRNA and ITS region sequencing to explore microbial community structure and diversity, predict bacterial and fungal functions, and track changes in beneficial and pathogenic microbes. Results showed that clubroot severity affects rhizosphere microbial diversity and community structure. The richness of rhizosphere bacteria was ranked as follows: disease-free &gt; mild &gt; severe, while that of rhizosphere fungi was ranked as follows: mild &gt; severe &gt; disease-free. Proteobacteria, Bacteroidota, and Firmicutes were the dominant bacterial phyla, while <em>Pseudomonas</em>, <em>Flavobacterium</em> and <em>Rhizobium</em> were the dominant bacterial genera in the soil samples tested. The dominant fungal phyla in rhizosphere soil were Olpidiomycota, Ascomycota and Chytridiomycota, while the dominant fungal genera were <em>Olpidium</em>, <em>Fusarium</em>, and <em>Plectosphaerella</em>. The occurrence of clubroot increased bacterial functions such as Environmental_Information_Processing and Cellular_Processes in the rhizosphere of oilseed rape, and decreased functions such as Organismal_Systems, Genetic_Information_Processing and Metabolism. Clubroot also increased the abundance of Saprotroph-type fungi and decreased the abundance of fungal/animal parasite-type fungi in the rhizosphere. Furthermore, as clubroot severity increased, the abundance of beneficial microorganisms in the rhizosphere exhibited a decreasing trend, while that of pathogenic microorganisms showed an increasing trend. Our findings demonstrated that the structure, composition and diversity of rhizosphere microbial communities were closely related to the occurrence and severity of clubroot, thus providing an effective approach to synthesizing beneficial microorganisms, in order to control clubroot, alleviate soil degradation, and rebuild healthy soils.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102294"},"PeriodicalIF":1.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas: From bacterial immunity to precision genome engineering","authors":"Sidra Abbas ,&nbsp;Ayesha Saeed ,&nbsp;Monaza Bibi ,&nbsp;Shaghufta Perveen ,&nbsp;Nosheen Masood","doi":"10.1016/j.genrep.2025.102296","DOIUrl":"10.1016/j.genrep.2025.102296","url":null,"abstract":"<div><div>The CRISPR-Cas system is an adaptive immune strategy in bacteria that has rapidly transformed into a cornerstone of modern genome engineering with broad applications in medicine, agriculture, and biotechnology. This review explores its molecular foundations and highlights its expanding functional scope, including roles in immunity, DNA repair, and transcriptional regulation. CRISPR technology has emerged as a powerful tool in addressing major global health challenges, enabling precise genome modifications in drug-resistant parasites such as <em>Plasmodium</em> and <em>Trypanosoma cruzi</em>, as well as offering promising strategies against persistent viral infections like HIV and hepatitis B. However, challenges remain, particularly the risk of off-target effects, which underscores the need for refined precision tools and innovative delivery methods. Recent advances are addressing these limitations through next-generation approaches such as engineered virus-like particles, DNA nanostructures, and synthetic exosomes are improving delivery efficiency and targeting specificity, while bioresponsive hydrogels offer controlled, site-specific release of editing components. At the same time, newer tools like prime editing systems and Cas12 variants are achieving highly accurate, double-strand break–free modifications with expanded targeting capacity. These advances point toward a safer, more adaptable future for genome editing. CRISPR's transformative impact will depend on improving delivery strategies, enhancing precision, and establishing robust ethical and regulatory frameworks. As applications expand, particularly in germline editing, ethical concerns surrounding intergenerational consent, human enhancement, and potential eugenic misuse become increasingly pressing. The possibility of non-therapeutic genetic modification further elevates the need for equitable access and strong global oversight to avoid deepening health disparities. The future of CRISPR lies in harmonizing scientific innovation with societal responsibility—ensuring that progress is both impactful and ethically grounded.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102296"},"PeriodicalIF":1.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The future of diabetes mellitus management: Therapeutic insights from gene and stem cell research","authors":"Snigdha Suman Das ,&nbsp;E. Vignesh Balaji ,&nbsp;K. Sreedhara Ranganath Pai","doi":"10.1016/j.genrep.2025.102292","DOIUrl":"10.1016/j.genrep.2025.102292","url":null,"abstract":"<div><h3>Aim</h3><div>This review will explore the therapeutic potential of gene and stem cell therapy in diabetes mellitus, focusing on the latest developments in CRISPR/Cas9 gene editing, stem cell generated pancreatic beta cells and the hybrid therapeutic approaches in between.</div></div><div><h3>Methods</h3><div>A thorough review of the literature was performed, with the focus being on the mechanisms of gene therapy and stem cell differentiation and their integration with bioengineering technologies. Furthermore, therapeutic targets were identified and clinical trial outcomes were reviewed to determine the translational feasibility of these approaches.</div></div><div><h3>Results</h3><div>Gene therapy has shown potential in regenerating pancreatic beta cells and modulating immune responses through CRISPR/Cas9 based editing and insulin gene transfer. Stem cell based therapies have successfully differentiated into glucose responsive beta cells, and have reduced the need for insulin and enhanced glycemic control. The hybrid gene and stem cell based approaches have shown better durability and immune compatibility in the results and the 3D bioprinting is gradually developing implantable pancreatic tissue constructs. However, there are still some problems to be addressed, including immune rejection, ethical issues and the lack of scalability.</div></div><div><h3>Conclusion</h3><div>Gene and stem cell based interventions are potentially transformative in the management of diabetes, acting on the underlying pathophysiology rather than on symptoms only. These therapeutic modalities when integrated with bioengineering innovations, hold promise for developing curative approaches. However, safety, efficacy and ethical considerations of these critical challenges must be addressed in order to enable widespread clinical implementation of these advancements.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102292"},"PeriodicalIF":1.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ankylosing spondylitis: An overview of pathophysiology and therapeutic landscape","authors":"Jashandeep Singh ,&nbsp;Jagdeep Kaur ,&nbsp;Samriti Dhawan","doi":"10.1016/j.genrep.2025.102290","DOIUrl":"10.1016/j.genrep.2025.102290","url":null,"abstract":"<div><div>Ankylosing spondylitis (AS) is a chronic inflammatory autoimmune disease belonging to a group of conditions called spondyloarthritis that predominantly affects the spine and lower back joints. It is characterized by inflammation in the spine, stiffness in sacroiliac joints and progressive loss of mobility. The precise etiology of the disease remains unknown. Current hypotheses regarding the pathogenesis of AS suggest that multiple mechanisms are involved in its development. The genetic predisposition linked to HLA-B*27 represents the earliest documented finding; approximately five decades ago, extensive research indicated that it is not the sole factor responsible for the onset of the disease. New insights have been reported into the role of non-MHC genes, molecular mimicry, the generation of arthritogenic peptides, misfolded HLA-B*27 dimers or oligomers, and immune dysregulation. Two major hallmarks of this immune-mediated disease are inflammation and abnormal bone formation; both serve as targets for current therapies. Advances in the understanding of its pathophysiology have led to the development of highly effective and targeted treatment. AS treatments encompass NSAIDs, biologics (such as tumour necrosis factor inhibitors, interleukin-17 inhibitors, and interleukin-23 inhibitors), both conventional and targeted synthetic DMARDs, as well as monoclonal antibody therapies. The present study aims to provide a comprehensive overview of the pathophysiology, genetic and immunological factors involved in the process, as well as therapeutic advances reported in recent years.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102290"},"PeriodicalIF":1.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the neuroprotection efficacy of marine sponge extract in Parkinson's disease using gene expression and molecular docking","authors":"G.B. Priyadharshini ,&nbsp;Sree Karani Kondapuram ,&nbsp;C. Jaynthy","doi":"10.1016/j.genrep.2025.102288","DOIUrl":"10.1016/j.genrep.2025.102288","url":null,"abstract":"<div><div>Parkinson's disease (PD) is one of the most prevalent neurological conditions. The pathogenesis of PD was thought to involve mitochondrial dysfunction and oxidative stress. In this study, PD was induced in zebrafish models, and the effectiveness of MS01 sponge extract in neuroprotective effects was assessed. In zebrafish models, rotenone was treated, MS01-mixed pellets were fed, sensitivity and motor function were tested, and the fish were euthanized. Histopathology and fluorescent microscopy images of the zebrafish brain were then analyzed. Gene expression study was performed to analyze the expression of the PINK1 and Parkin genes. The compound xenin identified by LC-MS/MS analysis was selected for <em>in silico</em> studies. Using molecular docking and modeling techniques, the gene expression of PINK1 and Parkin was also investigated. The analysis showed binding of the xenin peptide to the PINK1-ubiquitin complex may enhance the stability of the complex and increase the production of parkin, which reduces PD symptoms. This demonstrates the neuroprotective effects of the xenin peptide.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102288"},"PeriodicalIF":1.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the interaction of hsa-miR-193b-5p and hsa-circ-cnot11-0001 in multiple sclerosis disease through dual luciferase assay","authors":"Amirreza Pashapouryeganeh ,&nbsp;Elham Mohammed Khatrawi ,&nbsp;Zohreh Pajohesh ,&nbsp;Rosa Hosseinzadegan ,&nbsp;Khaterehsadat Monirvaghefi ,&nbsp;Seyed Abbas Pakmehr ,&nbsp;Narges Pourdeilami ,&nbsp;Hossein Gharedaghi ,&nbsp;Sayedeh Fatemeh Sadat Madani ,&nbsp;Ali Azarpey ,&nbsp;Mona Torkaman Cheh ,&nbsp;Asal Mir ,&nbsp;Elham Ramezannezhad ,&nbsp;Saminnaz Kazemi ,&nbsp;Alireza Azani ,&nbsp;Negin Saffarzadeh ,&nbsp;Haniyeh Ghasrsaz ,&nbsp;Asra Idani ,&nbsp;Nour Mohammad Panahi ,&nbsp;Nasibeh Sargazi Moghaddam ,&nbsp;Moein Ghasemi","doi":"10.1016/j.genrep.2025.102291","DOIUrl":"10.1016/j.genrep.2025.102291","url":null,"abstract":"<div><h3>Background</h3><div>Multiple sclerosis (MS) is a chronic autoimmune disorder characterized by dynamic neurodegeneration, basically affecting people between the ages of 20 and 40. Despite significant progress in MS research, little is understood about the underlying molecular mechanisms, particularly those related to epigenetic regulation. According to recent studies, non-coding RNAs (ncRNAs) are crucial epigenetic regulators that have a big impact on how multiple sclerosis develops and is controlled. Given the increasing interest in ncRNAs as biomarkers and therapeutic targets, it is more crucial than ever to look into their role in MS. Recent transcriptomic research showing that circRNAs and miRNAs express differently in neurological disorders makes this especially pertinent. In order to shed light on their possible regulatory role in MS, this study examines the expression levels of circ-cnot11-0001 and hsa-miR-193b-5p in patients and uses a dual luciferase assay to analyze their direct interaction.</div></div><div><h3>Methods</h3><div>The gene expression profile GSE61741 was used to select key miRNAs and circRNAs in MS. Expressions of hsa-miR-193b-5p and circ-cnot11-0001 were verified by RT-qPCR from blood samples of 30 MS patients and 30 controls. After that, we constructed pBud-EGFP plasmid with hsa-miR-193b-5p and psiCHECK-2 plasmid with circ-cnot11-0001. Their direct interaction was determined by measuring luciferase activity.</div></div><div><h3>Result</h3><div>Expression analysis revealed significant downregulation of hsa-miR-193b-5p and upregulation of hsa-circ-cnot11-0001 in MS patients compared to controls. Also, the results of the luciferase activity determined that the luciferase activity of the hsa-miR-193b-5p-pBud + cnot11-0001-WT group was significantly lower than that of the hsa-miR-193b-5p-pBud + cnot11-0001-MT group. So, circ-cnot11-0001 can direct interaction with hsa-miR-193b-5p.</div></div><div><h3>Conclusion</h3><div>The findings indicate a direct interaction between hsa-miR-193b-5p and circ-cnot11-0001 in MS. Therefore, circ-cnot11-0001 may sponge hsa-miR-193b-5p and dysregulate the expression of genes related to MS. In conclusion, the CNOT11-0001-hsa-miR-193b-5p network may serve as a combined biomarker for further investigation into MS.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102291"},"PeriodicalIF":1.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of carbapenemase-encoding genes in Acinetobacter baumannii isolates from Morocco: A systematic review (2019–2024)","authors":"Hanane El Hafa","doi":"10.1016/j.genrep.2025.102289","DOIUrl":"10.1016/j.genrep.2025.102289","url":null,"abstract":"<div><div><em>Acinetobacter baumannii</em> has emerged as a significant cause of nosocomial infections, particularly in intensive care units. Its remarkable ability to acquire resistance to a wide range of antibiotics, especially carbapenems, poses a serious global public health challenge. This systematic review aims to assess the distribution and molecular characteristics of carbapenemase-encoding genes in <em>A. baumannii</em> isolates from Morocco between 2019 and 2024. We reviewed indexed publications reporting molecular detection of carbapenemase genes, including blaOXA-23, blaOXA-51, blaNDM, blaGES, and blaSPM. blaOXA-23 was the most frequently detected gene, often found in association with blaOXA-51. Although less common, blaNDM, blaGES, and blaSPM were also reported, with evidence of co-detection of multiple resistance genes in some isolates. These findings underscore the urgent need for molecular surveillance and infection control strategies in Moroccan healthcare settings.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102289"},"PeriodicalIF":1.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring molecular genetics and gene editing approaches of peripheral neuropathies: A future treatment approach","authors":"Fatima Muzamil ,&nbsp;Muhammad Waseem Sajjad","doi":"10.1016/j.genrep.2025.102285","DOIUrl":"10.1016/j.genrep.2025.102285","url":null,"abstract":"<div><div>Peripheral neuropathies are conditions in which the nerves of the peripheral nervous system (PNS) become disrupted. A tingling sensation and apathy are frequent symptoms. These symptoms could be unpleasant and are usually followed by fatigue. The hallmarks of acquired peripheral neuropathy (APN) include metabolic issues, inflammatory reactions, and impairments in axonal communication. Presently, it is known that errors in genetic material that encode proteins with a wide variety of activities result in different forms of Charcot-Marie-Tooth diseases (CMT) and associated hereditary peripheral neuropathies. Among these are cytoskeletal proteins, adhesion molecules, gap-junction proteins, transcription factors, receptors for neurotropic factors, structural proteins related to forming compact myelin, proteins involved in signalling pathways, and proteins involved in concealing the cell division process. The prospects of developing effective medications for neuropathic pain depend on the potential to comprehend pain mechanisms. Molecular methods for treating neuropathic pain include RNAi and gene-based therapies. New methods of gene therapy for the management of Inherited peripheral neuropathic conditions have been made possible by the latest innovations in genetic modification technologies. Driven by both inherited and acquired causes, the increasing frequency of peripheral neuropathies calls for creative and exact molecular therapies. This study is particularly relevant as direct manipulation of neuropathy-related mutations made possible by modern gene editing technologies like CRISPR-Cas9 and prime editing now helps Development of next-generation treatments depends on an awareness of these techniques.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102285"},"PeriodicalIF":1.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}