Gene Reports最新文献

{"title":"Glutathione peroxidase 4 gene polymorphism and its association with periodontitis and type 2 diabetes mellitus in south Indian population","authors":"Usha Subbiah ,&nbsp;Kaniha Sivakumar ,&nbsp;Nihala Sidhic ,&nbsp;Athira Ajith ,&nbsp;Challa Raji Lalasa ,&nbsp;Bathala Sai Dharani ,&nbsp;Harini Venkata Subbiah ,&nbsp;Jeyanthi Rebecca ,&nbsp;Anitha Balaji","doi":"10.1016/j.genrep.2025.102421","DOIUrl":"10.1016/j.genrep.2025.102421","url":null,"abstract":"<div><h3>Background</h3><div>Periodontitis and Type 2 Diabetes Mellitus are chronic disorders with high prevalence in the population, both influenced significantly by genetic factors. Glutathione peroxidase 4, a selenoprotein, plays a key role in maintaining oxidative homeostasis.</div></div><div><h3>Objectives</h3><div>This study evaluated the association of the GPX<em>4</em> rs713041 single-nucleotide polymorphism in the 5′ untranslated region with periodontitis, T2DM, and their co-occurrence in a South Indian cohort.</div></div><div><h3>Methods</h3><div>The SNP rs713041 was analysed in 300 diseased individuals and 100 healthy controls using PCR-RFLP. Additionally, the impact of the wild-type and variant rs713041 on mRNA secondary structure was studied, and molecular docking was performed to analyse the interaction of <em>GPX4</em> with gingipain (Kgp).</div></div><div><h3>Results</h3><div>The results revealed a significant association (<em>P</em> &lt; 0.05) of the heterozygous genotype T/C of rs713041 with periodontitis, T2DM, and periodontitis with T2DM. The frequency of the variant CC genotype was significantly higher in periodontitis compared to healthy controls (1.07–9.64; <em>P</em> &lt; 0.05). The genotype frequencies for rs713041 adhered to the Hardy-Weinberg equilibrium in the study population (<em>P</em> &gt; 0.05). Thermodynamic analysis revealed that the free energy of the rs713041 variant (−274.97 kcal/mol) was higher than that of the wild type, suggesting reduced stability of the variant. Docking analysis showed a binding energy of −47.18 kcal/mol for the <em>-</em>gingipain interaction.</div></div><div><h3>Conclusion</h3><div>The SNP rs713041 in the 5’ UTR of the <em>GPX4</em> gene may serve as a significant prognostic marker for periodontitis and T2DM, highlighting its potential role in the pathogenesis of these diseases.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102421"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated genomic and transcriptomic analysis reveals molecular mechanisms underlying fruit cracking resistance in melon (Cucumis melo L.)","authors":"Jie Liu ,&nbsp;Shuai-dong Wu ,&nbsp;Wen-yu Pang ,&nbsp;Yan-liang Guo ,&nbsp;Ji-yuan Wang ,&nbsp;Hu Li ,&nbsp;Chun Liu ,&nbsp;Yu-peng Fan ,&nbsp;Zhao Xie ,&nbsp;Zhong-zhou Yang ,&nbsp;Hui-jun Zhang","doi":"10.1016/j.genrep.2026.102430","DOIUrl":"10.1016/j.genrep.2026.102430","url":null,"abstract":"<div><div>Fruit cracking is a critical factor influencing the quality and yield of melons. To elucidate its molecular mechanism, this study utilized L-CR from cracking-resistant melon germplasm and L-CS from cracking-susceptible melon germplasm as experimental materials. By integrating high-depth resequencing with transcriptome analysis, we systematically compared genomic variations and gene expression regulation between the two types. Phenotypic analysis revealed no significant difference in fruit size between the two melon types but demonstrated a marked disparity in cracking susceptibility. Genomic variation analysis identified numerous single nucleotide polymorphism (SNP) and insertion/deletion (InDel) loci, which exhibited significantly differential distributions between the two materials. Transcriptome analysis detected 6038 differentially expressed genes (DEGs) in the L-CS, including 2876 significantly upregulated genes and 3162 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that these DEGs were significantly enriched in hormone signal transduction pathways. Further investigation revealed that in the L-CS, the expression levels of key genes involved in auxin (IAA) and cytokinin (CTK) biosynthesis and signaling pathways were substantially elevated. Additionally, the key genes in the brassinosteroid (BR) biosynthesis pathway also displayed higher expression levels. These findings suggest that the coordinated activation of hormone pathways such as IAA, CTK, and BR plays a crucial role in melon fruit cracking. This study provides a foundational framework for understanding the molecular mechanisms underlying melon cracking and identifies potential target genes for breeding cracking-resistant varieties.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102430"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-intensity pulsed ultrasound modulating neuroinflammation by hippocampal gene expression in LPS-induced depression","authors":"Yuanli Wang ,&nbsp;Lijie Zhang ,&nbsp;Linxian Li ,&nbsp;Jingjie Wang ,&nbsp;Mingxue Wang ,&nbsp;Nannan Huang ,&nbsp;Paul K. Chu ,&nbsp;Hongrui Zhan ,&nbsp;Chengbiao Ding","doi":"10.1016/j.genrep.2026.102431","DOIUrl":"10.1016/j.genrep.2026.102431","url":null,"abstract":"<div><h3>Background</h3><div>Neuroinflammation is critically implicated in the pathogenesis of depression. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive neuromodulation technique with therapeutic potential, though its molecular mechanisms in depression remain unclear.</div></div><div><h3>Objective</h3><div>This study aims to elucidate the transcriptomic mechanisms underlying the effects of LIPUS in the hippocampus of lipopolysaccharide (LPS)-induced depressed mice.</div></div><div><h3>Methods</h3><div>We established an LPS-induced mouse model of depression and applied LIPUS to the hippocampus. Hippocampal transcriptomes were profiled by RNA-seq, and bioinformatic analyses identified key biological processes, pathways, and hub genes.</div></div><div><h3>Results</h3><div>LIPUS significantly reverses LPS-induced transcriptomic alterations. Enrichment analysis reveals that LIPUS modulated pathways are related to neuroinflammation and synaptic plasticity. Protein-protein interaction network analysis further identifies central hub genes governing these processes.</div></div><div><h3>Conclusion</h3><div>Our findings demonstrate that LIPUS mitigates depression-like phenotypes by reprogramming the hippocampal transcriptome, primarily through targeting neuroinflammatory and synaptic plasticity networks, revealing its potential as a novel therapeutic strategy.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102431"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emerging role of mitochondrial transcription termination factors (MTERFs) in tumorigenesis and progression in cancers","authors":"Jintao He ,&nbsp;Yaru Lin ,&nbsp;Wenbing Huang ,&nbsp;Ximin Tang ,&nbsp;Min Yu ,&nbsp;Zifeng Ruan ,&nbsp;Xingguo Liu ,&nbsp;Zhengliang Li ,&nbsp;Yanwen Wang ,&nbsp;Wei Xiong","doi":"10.1016/j.genrep.2025.102406","DOIUrl":"10.1016/j.genrep.2025.102406","url":null,"abstract":"<div><div>Mitochondrial transcription and its dysregulation are emerging as important factors in cancer biology, providing new insights into tumor metabolism and potential therapeutic targets. The protein family of human mitochondrial transcription termination factor (MTERF) is pivotal in controlling mitochondrial gene expression and oxidative phosphorylation (OXPHOS) activity. MTERF proteins impact mitochondrial metabolism and cell energy generation by regulating mtDNA replication, transcription, and involvement in assembling mitochondrial ribosomal subunits and synthesizing proteins. Growing research suggests that members of the MTERF family are crucial not just for regular cell function but also play a significant role in the onset and development of human cancers. The expression of these transcription factors is intimately linked to the growth, movement, and penetration of tumor cells, underscoring their possible significance in predicting clinical outcomes and treating cancer. Dysregulation of MTERFs directly leads to altered expression of mtDNA in cancer cells, resulting in cellular metabolic reprogramming and mitochondrial dysfunction. The article encapsulates the roles and fundamental processes of various MTERF family members across different tumor forms, seeking to lay a theoretical groundwork for additional studies on their involvement in tumor development, forecasting prognoses, and specific treatments.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102406"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of BRCA1/BRCA2 pathogenic variants implicated in prostate cancer by next-generation sequencing","authors":"Khaoula Elghazali ,&nbsp;Youssef Ennaji ,&nbsp;Abdelilah Laraqui ,&nbsp;Ahmed Ameur ,&nbsp;Moulay Mustapha Ennaji","doi":"10.1016/j.genrep.2025.102397","DOIUrl":"10.1016/j.genrep.2025.102397","url":null,"abstract":"<div><div>Prostate cancer poses a significant public health challenge worldwide, and its hereditary forms are increasingly recognized in clinical practice. Pathogenic variants in the BRCA1 and BRCA2 genes contribute to the risk of hereditary prostate cancer, but data from North African populations are still limited. This study examined germline variants in the BRCA1/2 genes in 30 Moroccan patients diagnosed with prostate cancer. Next-generation sequencing was used to analyze the full coding regions and intron-exon boundaries of both genes.</div><div>Two deleterious frameshift insertions were identified: BRCA1 c.3514_3515insT and BRCA2 c.4240_4241insA, each detected in two unrelated patients. These gene carriers had high-risk disease (tumor stage ≥ 2 and Gleason score ≥ 7). A BRCA2 variant of uncertain significance (c.681 + 56C &gt; T) was present in almost half of the cohort, alongside several intronic variants and benign polymorphisms. Importantly, almost half of the identified variants were absent from existing North African genomic databases.</div><div>These findings provide new insights into the spectrum of BRCA1/2 germline variants in prostate cancer and highlight the need for reference genomic data on ancestry. Broader inclusion of underrepresented populations will be essential to improve variant interpretation and guide precision medicine in hereditary prostate cancer worldwide.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102397"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PGK1 is a prognostic molecule and silencing PGK1 inhibits the growth in breast cancer cells","authors":"Zhenzhen Yang ,&nbsp;Ying Han","doi":"10.1016/j.genrep.2025.102396","DOIUrl":"10.1016/j.genrep.2025.102396","url":null,"abstract":"<div><h3>Background</h3><div>Due to its persistently high prevalence and mortality, breast cancer (BC) remains a significant public health challenge. The present study comprehensively investigates the role of PGK1 (Phosphoglycerate Kinase 1) in BC, recognizing its critical involvement in tumorigenesis and disease progression despite the scarcity of reports addressing its specific implications.</div></div><div><h3>Methods</h3><div>Genomic microarray datasets and associated clinicopathological records were acquired from two publicly accessible repositories: the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). Comprehensive comparative investigations were conducted, covering functional enrichment evaluation, characterization of the tumor immune microenvironment, and assessment of pharmacological responsiveness in BC. Experimental validation of PGK1's biological functions in BC was further carried out using both in vitro experimental models and in vivo animal systems. Statistical processing and graphical representations of data were primarily executed using the R programming environment.</div></div><div><h3>Results</h3><div>Experimental results revealed a marked elevation in PGK1 expression levels within BC tissues, correlating with unfavorable clinical outcomes. Clinical association studies identified substantial relationships between PGK1 abundance and critical pathological markers in BC, such as estrogen receptor (ER) positivity, progesterone receptor (PR) status, and human epidermal growth factor receptor 2 (Her-2) expression levels. Gene Set Enrichment Analysis (GSEA) demonstrated PGK1's prominent engagement in fundamental cellular mechanisms, particularly mitotic regulation and genomic replication processes. Additionally, systematic evaluation revealed significant co-variation patterns between PGK1 and immune checkpoint regulators. Pharmacogenomic assessments established proportional relationships between PGK1 expression and therapeutic responses to specific agents, including AT-7519, Methotrexate, and MS-275. In cell experiments, silencing PGK1 resulted in a marked decrease in cellular proliferation and migration abilities, accompanied by an increase in apoptosis levels. In nude mouse tumorigenesis experiments, PGK1 knockdown significantly slowed tumor growth. Overall, both in vitro and in vivo results demonstrate that reduced PGK1 expression markedly affects BC cell growth.</div></div><div><h3>Conclusions</h3><div>This study systematically validates PGK1's prognostic value, clinical associations, and functional relevance in BC, while integrating multi-omics data (transcriptomic, clinical, immune, and pharmacogenomic) to clarify its subtype-specific roles. The findings might position PGK1 as a high-priority therapeutic candidate for targeted intervention strategies against breast malignancies.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102396"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From microbes to medicine: How microbiota profiling is shaping the future of cancer therapy","authors":"Anna Abdolshahi ,&nbsp;Ramtin Naderian ,&nbsp;Hamed Tahmasebi ,&nbsp;Elham Paraandavaji ,&nbsp;Mohammad Amin Hemmati ,&nbsp;Setayesh Kashanipoor ,&nbsp;Majid Eslami","doi":"10.1016/j.genrep.2025.102402","DOIUrl":"10.1016/j.genrep.2025.102402","url":null,"abstract":"<div><div>Microbiota profiling is increasingly recognized as fundamental in advancing personalized cancer therapy. Beyond identifying microbial biomarkers, this review positions microbiota profiling as a dynamic and functional axis in precision oncology capable of both informing patient stratification and being therapeutically modulated to enhance treatment efficacy. By uncovering the dynamics of host-microbiota interactions, it sheds light on their impact on carcinogenesis, tumor progression, and treatment outcomes. This review synthesizes the latest advancements, ongoing challenges, and future directions for microbiome-based strategies in cancer treatment. This study adopts a narrative review approach, synthesizing findings from peer-reviewed articles indexed in PubMed, Web of Science, and Scopus, using a focused search strategy, as detailed in the methodology. The focus centers on microbiota profiling, its roles in cancer therapy, and related therapeutic implications. Microbiota profiling facilitates detailed characterization of microbial ecosystems and their involvement in cancer development and treatment. The next-generation sequencing, metabolomics, and machine learning have enhanced the discovery of microbial biomarkers. Emerging microbiota-focused interventions, including fecal microbiota transplantation and probiotic therapies, show promise in improving responses to immunotherapy and chemotherapy. However, clinical implementation faces hurdles such as interindividual variability, lack of standardization, and ethical complexities. Microbiota profiling represents a revolution for personalizing cancer treatments. This review synthesizes recent advances in microbiome-focused analytical technologies and examines their implications for cancer diagnosis, treatment, and immune modulation, highlighting key translational opportunities and future directions in oncology.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102402"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic basis of NMN and rapamycin in aging biology: Independent and intersecting pathways","authors":"C. Sasidhar ,&nbsp;V. Chitra ,&nbsp;V. Naveen Kumar","doi":"10.1016/j.genrep.2025.102401","DOIUrl":"10.1016/j.genrep.2025.102401","url":null,"abstract":"<div><div>Aging is a worldwide concern as populations are growing old, and there is increasing demand to find effective interventions capable of delaying the effects of age and thereby increasing healthspan. Nicotinamide mononucleotide (NMN) and rapamycin are two mechanistically different but potentially synergistic agents that target major hallmarks of aging, as part of emerging geroprotective. NMN is a precursor of NAD⁺, which activates sirtuins, maintains genomic stability, promotes mitochondrial activity; whereas rapamycin prevents activation of mTORC1 signaling through supporting autophagy and restricting cellular senescence and regulating immune response. This review has critically discussed the pharmacological molecular mechanism of NMN and rapamycin and their potential benefits in aging-associated pathways and preclinical and early clinical trials. We analyze the cross-communication between the NAD⁺/sirtuin and mTOR/autophagy axes, paying attention to the possibility of complementary regulation of the two systems to deliver synergistic effects against aging. Compared to other drugs that can be described as geroprotectors, including metformin, resveratrol, spermidine, and senolytics, NMN and rapamycin offer complementary mechanisms of action in a broader therapeutic context. To validate that this dual-pathway approach is safe and effective, we finally enumerate the challenges of translation dosing optimisation, biomarker development, and regulatory challenges. The association between NMN and rapamycin has shown a potential future in anti-aging medicine, as it has the potential to transform the paradigm of age-related disease therapy.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102401"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear ITS and chloroplast markers as complementary tools for molecular authentication of Piper betle Linn. landraces in South India","authors":"Gaanappriya Mohan ,&nbsp;Amala Kesavan ,&nbsp;Gowri Shankar Bagavananthem Andavan","doi":"10.1016/j.genrep.2026.102433","DOIUrl":"10.1016/j.genrep.2026.102433","url":null,"abstract":"<div><div><em>Piper betle</em> Linn. an economically and medicinally important species of the Piperaceae family, exhibits extensive regional variation in South Asia; however, accurate identification of its landraces is often confounded by morphological similarity and inconsistent local nomenclature. This study employed a multi-locus DNA barcoding approach to characterize six South Indian cultivars of <em>P. betle</em> collected from Anthiyur, Trichy, and Sargur. Three loci, the nuclear internal transcribed spacer (ITS) and the chloroplast intergenic spacers psbA-trnH and trnL-trnF, were amplified and sequenced. ITS sequences (640–676 bp) exhibited the greatest discriminatory power, resolving cultivars into distinct regional clades with evolutionary distances of up to 0.0031, whereas psbA-trnH (252–280 bp) showed moderate divergence, particularly between the Karnataka and Tamil Nadu accessions. The trnL-trnF region (882–913 bp) was highly conserved, effectively validating species identity but was unable to resolve intra specific variation. Phylogenetic analyses confirmed that all accessions formed a monophyletic cluster distinct from related <em>Piper</em> species, with ITS markers providing the clearest regional structure. These findings establish a reliable molecular framework for authenticating <em>P. betle</em> cultivars and supporting germplasm conservation and quality assurance in commercial applications. This study underscores the value of ITS-based barcoding, complemented by plastid markers, in unambiguously delineating region specific betel vine lineages.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102433"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights and growth type-specific expression of Lv14-3-3ζ in the Pacific white shrimp, Litopenaeus vannamei","authors":"Md Abu Hanif,&nbsp;Yusin Cho,&nbsp;Shaharior Hossen,&nbsp;Kang Hee Kho","doi":"10.1016/j.genrep.2025.102386","DOIUrl":"10.1016/j.genrep.2025.102386","url":null,"abstract":"<div><div>The proteins of the 14-3-3 family participate in diverse biological events by regulating diverse cell signaling pathways and the formation of protein-protein interactions. To understand the potential growth regulatory role of the 14-3-3 gene, the cDNA encoding <em>Lv</em>14-3-3ζ was isolated and characterized, and its tissue and growth-specific expression was analyzed in the Pacific white shrimp, <em>Litopenaeus vannamei</em>. The <em>Lv</em>14-3-3ζ cDNA was 2260 bp in length, containing a 741 bp open reading frame (ORF) encoding 246 putative amino acid residues flanked by a 75 bp 5′ untranslated region (UTR) and a 1443 bp 3′ UTR. Structurally, <em>Lv</em>14-3-3ζ is intronless and binds C-RAF phosphopeptide and fusicoccin ligands. Although this protein is predicted to have protein-succinylysine desuccinylase activity and protein-specific binding activity based on gene ontology, <em>Lv</em>14-3-3ζ is a multifunctional protein that interacts with diverse known and unknown proteins. <em>Lv</em>14-3-3ζ showed differential expression in tissue samples of the Pacific white shrimp, with the highest abundance in the digestive gland (<em>p</em> &lt; 0.001), followed by lower expression in the ovary, intestine, and muscle, and low levels in the heart, nerve cord, and gill. A decreasing expression pattern of <em>Lv</em>14-3-3ζ was exhibited by slow to rapid-growth individuals, whereas myosin heavy chain (MHC) showed an increasing expression trend. The results of the present study provide comprehensive information on <em>Lv</em>14-3-3ζ gene, its structure and highlight its potential as a growth regulator in the Pacific white shrimp.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"42 ","pages":"Article 102386"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}