Genomic insights and biotechnological potential of “Mesobacillus aurantius” strain S13: A canthaxanthin pigment-producing bacterium

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-07-12 DOI:10.1016/j.genrep.2025.102287

Shankar Karthik , Gurusamy Raman , Arumugam Srinivasan , Syed Gulam Dastager , Vasudev Wagh , Niraikulam Ayyadurai

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引用次数: 0

Abstract

This study explores the discovery and characterization of pigments, from a microorganism thriving under environmentally challenging conditions. Utilizing a polyphasic taxonomic approach, we successfully isolated and identified a bacterial strain S13 within the Mesobacillus genus and Bacillaceae family, designated as “Mesobacillus aurantius” S13. We confirmed the species based on comprehensive phenotypic, physiological, and biochemical analyses, molecular phylogenetics, DNA–DNA hybridization, average nucleotide identity, and whole-genome sequencing. The strain S13 (MTCC13141 = VKM B-3583 = NBIMCC 9084) possesses a 4.81 Mbp genome that encodes genes for pigment production. Notably, this study uses various spectroscopic methods to characterize canthaxanthin, an orange pigment and delineates its biosynthetic pathway from the genome of S13. The methylerythritol phosphate (MEP) pathway and canthaxanthin biosynthesis genes highlight the capacity of the bacterium to synthesize crucial isoprenoids and terpenoids, including canthaxanthin. This research provides a detailed account of the isolation, characterization, and taxonomic classification of “Mesobacillus aurantius” S13 and underscores the potential biotechnological applications of the bacterium in producing pharmaceuticals, biofuels, and cosmetics. Therefore, “Mesobacillus aurantius” S13 is a potentially valuable microbial resource for the industrial production of isoprenoids and pigments.

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“金黄色中杆菌”菌株S13：一种产生角黄素色素的细菌的基因组学见解和生物技术潜力

本研究探讨了色素的发现和表征，从微生物繁荣的环境挑战条件下。利用多相分类学方法，成功分离鉴定了一株菌株S13，命名为“Mesobacillus aurantius”S13。我们根据综合表型、生理生化分析、分子系统发育、DNA-DNA杂交、平均核苷酸鉴定和全基因组测序证实了该物种。菌株S13 （MTCC13141 = VKM B-3583 = NBIMCC 9084）拥有一个4.81 Mbp的基因组，编码色素产生基因。值得注意的是，本研究使用了各种光谱方法来表征橙色色素canthaxanthin，并从S13基因组中描绘了其生物合成途径。甲基赤藓糖醇磷酸（MEP）途径和角黄素生物合成基因强调了细菌合成关键的类异戊二烯和萜类物质的能力，包括角黄素。本研究详细介绍了“金黄色中杆菌”S13的分离、特性和分类，并强调了该细菌在生产药品、生物燃料和化妆品方面的潜在生物技术应用。因此，“金中杆菌”S13是工业生产类异戊二烯和色素的潜在有价值的微生物资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.