Gastroenterology Research and Practice最新文献

{"title":"Use of Modified YOLOv5 Algorithm in the Differential Diagnosis of Colonic Crohn's Disease and Ulcerative Colitis on CTE Images.","authors":"Mingbo Bao, Wenjia Liu, Haifeng Shi, Mingzhu Meng, Jian Cao","doi":"10.1155/grp/1506567","DOIUrl":"10.1155/grp/1506567","url":null,"abstract":"<p><p><b>Background:</b> Inflammatory bowel disease (IBD) is an immune-mediated disorder characterized by intestinal inflammation and includes two subtypes: Crohn's disease (CD) and ulcerative colitis (UC). The computed tomography manifestations of colonic CD (cCD) and UC are similar, and differential diagnosis is challenging. Our study aimed to investigate the feasibility of using a modified YOLOv5 algorithm for differentiating between cCD and UC on computed tomography enterography (CTE) images. <b>Methods:</b> This multicenter retrospective study analyzed data from a total of 29 cCD patients and 29 UC patients. Five submodels (YOLOv5n, YOLOv5s, YOLOv5m, YOLOv5l, and YOLOv5x) of YOLOv5 were trained and evaluated on the datasets. The CTE images of the cCD group and UC group were divided into a training set, validation set, and test set at a ratio of 8:1:1. Finally, the precision (Pr), recall rate (Rc), and mean average precision (mAP_{_0.5} and mAP_{_0.5:0.95}) of the models were compared. <b>Results:</b> The YOLOv5x model showed the best performance among the five submodels, with mAP_{_0.5} of 0.97 and mAP_{_0.5:0.95} of 0.97 and 0.84 in the validation set and mAP_{_0.5} and mAP_{_0.5:0.95} of 0.97 and 0.83 in the test set, respectively. These results demonstrated similar diagnostic accuracy to the two radiologists (84.5%). <b>Conclusion:</b> The modified YOLOv5 algorithm is a feasible approach to distinguish between cCD and UC on CTE images. These findings may facilitate the early detection and differential diagnosis of IBD.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"1506567"},"PeriodicalIF":1.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12310326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Expression of MicroRNAs in the Colorectal Serrated Neoplasia Pathway and Adenoma-Carcinoma Sequence.","authors":"Takashi Murakami, Hiroyuki Mitomi, Naoki Tsugawa, Yudai Otsuki, Eiji Kamba, Yuichiro Kadomatsu, Takuo Hayashi, Tsuyoshi Saito, Tomoyoshi Shibuya, Takashi Yao, Akihito Nagahara","doi":"10.1155/grp/1010891","DOIUrl":"10.1155/grp/1010891","url":null,"abstract":"<p><p><b>Background and Aim:</b> Colorectal carcinogenesis involves two distinct pathways, the serrated neoplasia pathway and adenoma (AD)-carcinoma sequence, whose precursors are sessile serrated lesion (SSL) and traditional AD, respectively. MicroRNAs (miRNAs) regulate gene expression and play a crucial role in colorectal tumorigenesis. This study investigated miRNA expression in the precursors and early invasive carcinomas of the two pathways. <b>Methods:</b> Using real-time reverse transcription polymerase chain reaction, we quantified the expression of miR-20a, miR-21, miR-93, and miR-181b in 127 lesions, including 25 SSLs, 19 SSLs with high-grade dysplasia (SSL-HD), 13 SSLs with submucosal invasive carcinoma (SSL-SC), 19 ADs, 26 ADs with HD (AD-HD), and 25 ADs with SC (AD-SC). <b>Results:</b> In the SSL series, miR-93 (SSL vs. SSL-SC, <i>p</i> = 0.038) and miR-181b (SSL vs. SSL-HD/SSL-SC, <i>p</i> = 0.013/<i>p</i> < 0.001, respectively) levels decreased with tumor progression. In the AD lineage, the expression of miR-20a (AD vs. AD-SC and AD-HD vs. AD-SC, <i>p</i> < 0.001), miR-21 (AD vs. AD-HD/AD-SC and AD-HD vs. AD-SC, <i>p</i> < 0.001), and miR-181b (AD-HD vs. AD-SC, <i>p</i> = 0.020) increased during carcinogenesis. Compared with normal mucosa (baseline), miR-93 expression showed a stepwise increase with tumor progression in the AD lineage, whereas the values did not change during SSL carcinogenesis. In the AD lineage, miR-20a expression increased in early invasive carcinoma but decreased in this phase of the SSL series. Overall, miR-20a, miR-93, and miR-181b levels were significantly lower in SSL-SC than in AD-SC (all <i>p</i> < 0.001). <b>Conclusions:</b> These findings indicate that the SSL and AD pathways exhibit distinct miRNA expression dynamics during colorectal tumorigenesis, with the AD lineage showing a progressive increase in oncogenic miRNAs and the SSL series exhibiting selective downregulation or plateauing, particularly in invasive lesions. The differential expression of miR-20a, miR-21, miR-93, and miR-181b was presumed to be related to (epi)genetic alterations among serrated neoplasia and AD-carcinoma routes.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"1010891"},"PeriodicalIF":2.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of Biomarkers and Immunotherapy With Crohn's Disease Using WGCNA and Two-Sample Mendelian Randomization Study.","authors":"Cong Hu, Shuxiong Nong, Chenang Liu, Yongfeng Chen, Chilin Liao, Meng Wu","doi":"10.1155/grp/8194480","DOIUrl":"10.1155/grp/8194480","url":null,"abstract":"<p><p><b>Objective:</b> Crohn's disease (CD) is a chronic systemic inflammatory disease that mainly affects the intestine, accompanied by extraintestinal symptoms and immune problems. The progression of the disease may cause permanent damage to the structure and function of the intestine. Due to unclear early symptoms and lack of precise detection methods, early diagnosis of CD is difficult. Many patients were diagnosis at late stage, which may lead to delayed treatment and increased risk of complications. Identifying hub genes related to CD and using them to predict CD is of great significance. <b>Methods:</b> DEG and WGCNA were employed to identify key genes associated with CD and to detect modules significantly linked to the disease. GO and KEGG analyses were conducted to explore the functions of these identified genes. Additionally, MR method was utilized to assess the causal relationships between the most significant gene and CD. <b>Results:</b> WCGNA identified 3240 differentially expressed genes, with the magenta module being the most significant among the nine clustered modules. The enrichment of GO and KEGG pathways indicates that the hub genes in the magenta module are related to the positive regulation of heme binding, tetrapyrrole binding, carboxylic acid binding, organic acid binding, IL-17 signaling pathway, and amoebiasis pathway. The Top 5 hub genes are CXCL1, LCN2, NOS2, S100A8, and DUOX2. Mendelian randomization analysis found a significant correlation between CXCL1 and CD. <b>Conclusions:</b> The study screened five potential biomarker genes in CD patients using a bioinformatics approach and Mendelian randomization study. Our results provided insights into CXCL1, LCN2, NOS2, S100A8, and DUOX2 in CD and suggested that CXCL1 may potentially be the optimal biomarker that could be a relatively easy path to clinical translation.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"8194480"},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological and Genetic Features in Superficial Nonampullary Duodenal Epithelial Tumors.","authors":"Atsushi Sawada, Kingo Hirasawa, Makoto Sugimori, Yuichiro Ozeki, Ryosuke Ikeda, Masafumi Nishio, Takehide Fukuchi, Ryosuke Kobayashi, Hiroaki Kaneko, Chiko Sato, Yoshiaki Inayama, Chikara Kunisaki, Shin Maeda","doi":"10.1155/grp/1063863","DOIUrl":"10.1155/grp/1063863","url":null,"abstract":"<p><p><b>Background and Aim:</b> Superficial nonampullary duodenal epithelial tumors (SNADETs) that are pathologically classified as gastric-type might manifest a more aggressive behavior than the intestinal type. However, the details of their histologic and genetic features remain unclear because of their rarity. This study was aimed at identifying clinicopathological findings and early genomic events in gastric-type SNADETs treated with endoscopic resection. <b>Methods:</b> We retrospectively analyzed 204 patients with SNADETs between January 2011 and September 2020. Immunohistochemical analysis for <i>β</i>-catenin and targeted exome sequence analysis of 50 cancer-related genes using next-generation sequencing were performed for the representative cases. <b>Results:</b> Among the 204 SNADETs cases, only nine (4.4%) were gastric type; the remaining 195 cases were intestinal type. Among the gastric-type tumors, seven were adenomas and two were adenocarcinomas, whereas only three of the 195 intestinal-type tumors were adenocarcinomas. Nuclear expression of <i>β</i>-catenin was observed in three of the nine (33%) gastric-type tumors and in eight of the 10 (80%) intestinal-type tumors. The most prevalent abnormality among the 50 genes tested in gastric-type tumors was <i>GNAS</i> mutation (89%), whereas that in intestinal-type tumors was <i>APC</i> mutation (67%). All gastric-type adenocarcinomas had <i>GNAS</i> mutations as well as adenomas, while <i>APC</i> mutations were absent in intestinal-type adenocarcinomas and present in most adenomas. <b>Conclusions:</b> <i>GNAS</i> mutations are more common in gastric-type SNADETs than in the intestinal type. As <i>GNAS</i> mutations are continuously present from adenoma to adenocarcinoma, resection at the adenoma stage is desirable.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"1063863"},"PeriodicalIF":2.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changrun Formula Relieves Functional Constipation by Improving Intestinal Motility in Rats.","authors":"Qiuping Xiao, Yanqiu Hong, Xuesi Geng","doi":"10.1155/grp/5790162","DOIUrl":"10.1155/grp/5790162","url":null,"abstract":"<p><p><b>Background and Study Aim:</b> Changrun Formula (CRF) is a representative traditional Chinese medicine prescription for functional constipation (FC). However, the mechanism by which CRF alleviates FC remains unclear. Therefore, this study aimed to investigate the therapeutic mechanism of CRF in an FC rat model. <b>Material and Methods:</b> A total of 72 healthy SD rats were selected and randomly divided into six groups: the blank group, model group, hemp seed pill (HSP) group, high-dose CRF group, medium-dose CRF group, and low-dose CRF group. Except for the blank group, all the other groups were administered compound diphenoxylate via oral gavage to establish the FC rat model with impaired intestinal motility. The expression of genes related to intestinal motility in the colon tissues of rats was analyzed using Western blotting and real-time PCR. The effect of CRF on isolated colonic smooth muscle was assessed through electrophysiological analysis. <b>Results:</b> Compared with the blank group, the other groups exhibited a longer time to expel the first black stool and a reduced number of fecal particles within 6 h, confirming the successful establishment of the FC rat model. Furthermore, the expressions of HCN1, c-kit, and SP in the colon tissue of the model group were significantly decreased, while the expression level of VIP was significantly increased. HCN1 was found to colocalize with c-kit, SP, and VIP. Treatment of CRF (high and medium doses) significantly increased the expressions of c-kit, SCF, HCN1, and HCN2, enhanced the contractile movement of colonic smooth muscle, and improved muscle tension. <b>Conclusions:</b> CRF likely improves intestinal motility by targeting HCN1 and HCN2 ion channels and the SCF/c-kit signaling pathway, thereby alleviating FC symptoms in rats.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"5790162"},"PeriodicalIF":2.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal Calprotectin as a Biomarker of Crohn's Disease in Patients With Short Disease Durations: A Prospective, Single-Center, Cross-Sectional Study.","authors":"Natsuki Ishida, Shunya Onoue, Tomohiro Takebe, Kenichi Takahashi, Yusuke Asai, Satoshi Tamura, Tomoharu Matsuura, Mihoko Yamade, Moriya Iwaizumi, Yasushi Hamaya, Takanori Yamada, Satoshi Osawa, Ken Sugimoto","doi":"10.1155/grp/9984055","DOIUrl":"https://doi.org/10.1155/grp/9984055","url":null,"abstract":"<p><p><b>Purpose:</b> Fecal calprotectin (FC) is a Crohn's disease (CD) biomarker, although the impact of disease duration on its accuracy remains unclear. This study was aimed at investigating the effects of CD disease duration on FC. <b>Methods:</b> In this prospective, single-center, cross-sectional study, we performed 113 endoscopies and biomarker measurements. Endoscopy results were assessed using the simple endoscopic score for Crohn's disease (SES-CD), with an SES-CD ≤ 2 defined as endoscopic remission (ER). Cohort 1 was divided into short-term and long-term disease groups. The associations of the SES-CD with C-reactive protein and FC were analyzed. <b>Results:</b> The correlation coefficient of FC and the SES-CD was 0.670 for all cases. In Cohort 1, the correlation coefficient of FC and the SES-CD was > 0.670 for all subgroups of the short-term disease group (≤ 20 years). The correlation coefficient of FC and CD was < 0.670 for all subgroups of the long-term disease group (> 20 years). In Cohort 2, the correlation coefficients were > 0.670 (0.808) for the 0-4-year disease group and < 0.670 for the 5-14- and 15-40-year disease groups. The receiver-operating characteristic analysis performed to predict ER of all cases resulted in an area under the curve (AUC) of 0.8443, with large AUCs of 0.907, 0.816, and 0.770 observed for the 0-4-, 5-14-, and 15-40-year disease groups, respectively. <b>Conclusions:</b> FC was affected by CD duration, and it may be a useful biomarker of CD, especially in patients with a short disease duration.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"9984055"},"PeriodicalIF":2.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Relative Measurement of Oxidative Stress in NAFLD Through Cyclic Voltammetry Method for Clinical Translation.","authors":"Dixa Sharma, Bhalendu S Vaishnav, Nupur Pandya, Pratik Pataniya, C K Sumesh, Palash Mandal","doi":"10.1155/grp/9948444","DOIUrl":"https://doi.org/10.1155/grp/9948444","url":null,"abstract":"<p><p>A potential contributing factor in the development of various metabolic diseases such as nonalcoholic fatty liver disease (NAFLD) could be oxidative stress and the production of reactive oxygen radicals. A high level of lipid peroxidation, including oxidative stress, can cause irreversible effects. We investigated the consequences of NAFLD on the reducing power of the liver in patients through plasma antioxidant capacity using screen-printed electrodes (SPEs). The study includes a total of 67 patient's population with steatosis (<i>n</i> = 29) and steatohepatitis (<i>n</i> = 38). Anodic current intensity (<i>la</i>), anodic wave area (<i>S</i>), and the biological sample oxidation potentials can be determined via cyclic voltammetry (CV) analysis. The enzyme glutathione peroxidase (GPx) and products of oxidative damage such as malondialdehyde (MDA), advanced glycation-end product (AGE), total status of oxidants (TOS), nitric oxide (NO), and cytokines analysis (qRT-PCR) of key mediators such as PNPLA3 in lipid metabolism, TIMP1 in fibrosis, and proinflammatory cytokines like NF-<i>κ</i>B, TNF-<i>α</i>, and IL-6, which are crucial for understanding NAFLD progression were recorded to further validate the CV obtained results along with and morphological changes through scanning electron microscope (SEM). The developed method measured oxidative stress with an error of less than 1.3% in human plasma samples, wherein the steatohepatitis caused a spike modification in the anodic current AC₅₂₀ and AC₉₇₂ (<i>p</i> < 0.01) compared to healthy humans. The presented electroanalytical methodology could be widely used for easy and rapid subjects' disease status detection. In addition to monitoring the response of subjects to treatment and providing nutritional supplements, these results may also be used for screening specific populations.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"9948444"},"PeriodicalIF":2.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and Outcomes of Upper GI Bleeding in Hospitalized SARS-CoV-2 Patients.","authors":"Erin Sanzone, Katherine Gheysens, Krystal Hunter, Adib Chaaya, Sangita Phadtare","doi":"10.1155/grp/4358786","DOIUrl":"10.1155/grp/4358786","url":null,"abstract":"<p><p><b>Background:</b> In March 2020, the severe acute respiratory distress syndrome coronavirus 2 (COVID-19) became a worldwide pandemic. Recently, it has been shown that direct entry of this virus in the gastrointestinal (GI) epithelial cells causes tissue damage and the use of anticoagulants increases the risk of GI bleeding. These pose real concerns for the gastroenterologists concerning the mortality, overall incidence, and management of upper GI bleeding in SARS-CoV-2-positive patients. <b>Methods:</b> This retrospective study includes patients 18 years or older admitted to our health system with an upper GI bleed (UGB). Patients with possible UGB, endoscopy, and SARS-CoV-2-positive testing (<i>n</i> = 587) formed the initial cohort. In-depth data were collected for symptoms, medications, source of bleeding, and interventions for subsets of test and control subjects. <b>Results:</b> Duodenal ulcer was the most common etiology for GI bleeding in SARS-CoV-2-positive patients, while esophagitis was the most common etiology in control subjects. SARS-CoV-2-positive patients had significant progressive anemia and had to be given more blood transfusions, steroids, proton pump inhibitors, and immunosuppressants. In-hospital mortality was greater in the experimental group (12.8%) than in the control group (5.1%). Furthermore, the SARS-CoV-2-positive patients had more therapeutic interventions compared to the SARS-CoV-2-negative patients. Nearly one-quarter of all patients had an endoscopy over 48 h after bleeding was observed. <b>Conclusions:</b> Healthcare providers should be aware of the greater therapeutic needs of SARS-CoV-2-positive patients with UGB. Our data helps shed light on the relationship between SARS-CoV-2 and GI bleeding due to SARS-CoV-2-related tissue damage and treatment affecting the GI tract.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"4358786"},"PeriodicalIF":2.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11976044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil Extracellular Traps Promote Pancreatic Cancer Progression via the STING Pathway.","authors":"Mengdi Qu, Chenyu Zhu, Caihong Sun, Shuainan Zhu, Hao Zhang, Changhong Miao, Di Zhou","doi":"10.1155/grp/4950214","DOIUrl":"10.1155/grp/4950214","url":null,"abstract":"<p><p><b>Background:</b> Pancreatic cancer is very susceptible to metastasis with a high mortality. Neutrophil extracellular traps (NETs) have been reported to be associated with poor prognosis in patients suffering from pancreatic cancer. However, the underlying mechanisms by which NETs facilitate cancer progression remain poorly understood. <b>Methods:</b> The expression of NETs was assessed in pancreatic cancer tissues and plasma samples from patients. Neutrophils were isolated from the blood of individuals diagnosed with pancreatic cancer to evaluate NETs formation. The impact of NETs on the progression of pancreatic cancer cells was investigated, along with a series of experiments aimed at elucidating the interaction mechanisms between neutrophils and cancer cells. <b>Results:</b> Pancreatic cancer samples had higher levels of NETs, and NETs formation was intensified in neutrophils derived from patients. NETs significantly promoted both migration and invasion capabilities in pancreatic cancer cells. Furthermore, the stimulator of interferon genes (STING) signaling pathway was stimulated to produce interleukin-8 (IL-8), which subsequently recruited more neutrophils and mediated further formation of NETs. <b>Conclusions:</b> Our data indicate a NETs-cancer aggressive crosstalk in pancreatic cancer. Specifically, NETs stimulate tumor cells to secrete IL-8, thereby promoting NETosis within the tumor microenvironment. Consequently, NETs may be a key target for pancreatic cancer treatment.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"4950214"},"PeriodicalIF":2.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Association of Negative Lymph Nodes With Adjuvant Chemotherapy in Patients With T3N0 Rectal Cancer.","authors":"Dongxu Lei, Zhanzhen Liu, Xinyi Kang, Ziwei Zeng, Hao Xie, Tanxing Cai, Fujin Ye, Li Xiong, Wenxin Li, Zhenxing Liang, Xiaobin Zheng, Shuangling Luo, Huashan Liu","doi":"10.1155/grp/3241615","DOIUrl":"10.1155/grp/3241615","url":null,"abstract":"<p><p><b>Background:</b> The use of adjuvant chemotherapy in patients with stage T3N0 rectal cancer following total mesorectal excision (TME) is debated. This study is aimed at investigating the clinical significance of negative lymph node (NLN) counts in patients with T3N0 rectal cancer, particularly in relation to adjuvant chemotherapy. <b>Methods:</b> This retrospective analysis examined 311 patients with T3N0 rectal cancer who underwent radical resection at the Sixth Affiliated Hospital of Sun Yat-sen University between August 2014 and December 2021. The optimal cutoff for NLN counts was determined using receiver operating characteristic (ROC) curves. Clinicopathological characteristics and clinical outcomes were compared between the high and low NLN groups. Overall survival (OS) and disease-free survival (DFS) were used to evaluate the efficacy of adjuvant chemotherapy. <b>Results:</b> The optimal cutoff for NLNs was 21. Of the 311 patients, 141 were categorized into the high NLN group and 170 into the low NLN group. Patients with NLNs ≥ 21 had significantly better 5-year OS (99.3% vs. 88.2%, <i>p</i> < 0.05) and 5-year DFS (92.2% vs. 79.4%, <i>p</i> < 0.05) compared to those with low NLNs. Multivariate Cox analysis revealed that NLN count was an independent prognostic factor for OS (hazard ratio (HR) = 0.078, 95% confidence interval (CI): 0.011-0.582, <i>p</i> = 0.013) and DFS (HR = 0.417, 95% CI: 0.213-0.815, <i>p</i> = 0.011). Subgroup analysis indicated that adjuvant chemotherapy significantly improved OS (<i>p</i> < 0.05) and DFS (<i>p</i> < 0.05) in the low NLN group. <b>Conclusion:</b> NLN count is an independent prognostic factor in patients with T3N0 rectal cancer. Patients with low NLN counts (NLN < 21) may benefit from adjuvant chemotherapy.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"3241615"},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}