Genetics and Molecular Biology最新文献_第5页

The hologenome concept and its implications for animal breeding in the multi-omics Era. 全基因组概念及其对多组学时代动物育种的影响。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0241

Tainã Figueiredo Cardoso, Luciana Correia Almeida Regitano

引用次数: 0

Fungal peptidogalactomann as a biocontrol agent Against Meloidogyne incognita in cotton. 真菌肽半乳甘露聚糖对棉花褐裂丝虫的生物防治作用。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-25 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0162

Maria Eugênia Lisei-de-Sa, José Leonardo Santos-Jiménez, Caroline de Barros Montebianco, Mariana C Bernardino, Mateus M Santos, Mariana Ingrid D da S Xisto, Andreia D Santino, Marcelo B Paganella, Nelson G Oliveira, Carolina V Morgante, Eliana Barreto-Bergter, Maria Fatima Grossi-de-Sa, Maite F S Vaslin

{"title":"Fungal peptidogalactomann as a biocontrol agent Against Meloidogyne incognita in cotton.","authors":"Maria Eugênia Lisei-de-Sa, José Leonardo Santos-Jiménez, Caroline de Barros Montebianco, Mariana C Bernardino, Mateus M Santos, Mariana Ingrid D da S Xisto, Andreia D Santino, Marcelo B Paganella, Nelson G Oliveira, Carolina V Morgante, Eliana Barreto-Bergter, Maria Fatima Grossi-de-Sa, Maite F S Vaslin","doi":"10.1590/1678-4685-GMB-2025-0162","DOIUrl":"10.1590/1678-4685-GMB-2025-0162","url":null,"abstract":"Root-knot nematodes (Meloidogyne incognita) severely limit cotton (Gossypium spp.) productivity, with conventional control methods facing ecological and efficacy challenges. Here, we evaluate a fungal peptidogalactomann (pGM), derived from Cladosporium herbarum (commercially formulated as Hariman), as a novel biocontrol agent that primes plant defenses against nematode infection. Under greenhouse conditions, foliar application of pGM at 100 μg·mL⁻¹ resulted in an 83% reduction gall formation compared to untreated controls and yielded a four-fold increase in resistant plants (gall index = 1.5 vs. 3.2 in controls; *p* < 0.01). Nematode egg production was reduced by 60% at 120 days post-inoculation. pGM triggered a biphasic defense response, evidenced by a remarkable 40,000-fold upregulation of the systemic acquired resistance gene PR1 and transient activation of phenylpropanoid (PAL) and jasmonate (LOX2) pathways. Notably, pGM supported healthy plant growth without phytotoxicity, contrasting with the phytotoxicity often associated with chemical nematicides. These findings indicate that pGM has strong potential as a sustainable alternative for M. incognita management in cotton, primarily by enhancing host immunity rather than exerting direct nematode toxicity. Further field trials are necessary to validate its effectiveness and to evaluate its integration into integrated pest management strategies.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 3 Suppl 3","pages":"e20250162"},"PeriodicalIF":1.7,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13203359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148029063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human microbiome in cancer: Not just a sidekick anymore. 癌症中的人类微生物群：不再只是一个伙伴了。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0236

Jennifer Vieira Gomes, Suzana Pinheiro de Oliveira Ribeiro, Gabriella Marins Cassiano do Nascimento, Danielle Oliveira Dos Santos, Diego José Gomes de Paula, Sheila Coelho Soares Lima, Tatiana de Almeida Simão

{"title":"The human microbiome in cancer: Not just a sidekick anymore.","authors":"Jennifer Vieira Gomes, Suzana Pinheiro de Oliveira Ribeiro, Gabriella Marins Cassiano do Nascimento, Danielle Oliveira Dos Santos, Diego José Gomes de Paula, Sheila Coelho Soares Lima, Tatiana de Almeida Simão","doi":"10.1590/1678-4685-GMB-2025-0236","DOIUrl":"10.1590/1678-4685-GMB-2025-0236","url":null,"abstract":"The human microbiome is increasingly recognized as a dynamic element in cancer biology. Studies across breast, prostate, lung, colorectal, and cervical tumors reveal that microbial communities influence carcinogenesis, immune regulation, and treatment outcomes. When the balance of these microorganisms is altered, inflammation becomes chronic, metabolism is disrupted, and signaling pathways such as NF-κB, IL6-STAT3, and β-catenin are activated. Bacterial metabolites and genotoxins, including colibactin and bile acids, may damage DNA and reshape the epigenetic landscape. Distinct microbial profiles have been linked to prognosis and to patient responses to chemotherapy and immunotherapy. The presence of beneficial taxa, such as Akkermansia muciniphila and Ruminococcus, has been associated with improved response to immune checkpoint inhibitors. At the same time, antibiotic-induced depletion of gut microbiome can reduce therapeutic efficacy. Strategies that help restore microbial balance, including probiotics, dietary interventions, and fecal microbiota transplantation, are being explored as complementary therapies. Although methodological differences and contamination remain challenges, the growing body of evidence indicates that the microbiome is a measurable and modifiable component of tumor ecosystems with strong potential for diagnostic, prognostic, and therapeutic applications in precision oncology.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250236"},"PeriodicalIF":1.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13196783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetics of Neotropical fishes: Patterns, advances and prospects after five decades of research. 新热带鱼类的细胞遗传学：五十年研究的模式、进展与展望。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0190

Mauro Nirchio, Claudio Oliveira

{"title":"Cytogenetics of Neotropical fishes: Patterns, advances and prospects after five decades of research.","authors":"Mauro Nirchio, Claudio Oliveira","doi":"10.1590/1678-4685-GMB-2025-0190","DOIUrl":"10.1590/1678-4685-GMB-2025-0190","url":null,"abstract":"This review synthesizes the most significant advances and innovations in the cytogenetic study of Neotropical fishes, with emphasis on recurrent chromosomal patterns, evolutionary mechanisms, and the integration of modern genomic tools. The Neotropical region, which harbors more than 12,000 fish species, exhibits not only extraordinary taxonomic richness but also remarkable chromosomal diversity. Chromosomal rearrangements have been associated with speciation and ecological adaptation in both freshwater and marine lineages. Modern techniques, including fluorescence in situ hybridization, chromosome painting, and comparative genomic hybridization (CGH), have substantially improved our ability to resolve morphologically indistinguishable species and clarify their evolutionary relationships. When integrated with phylogenetic and genomic approaches, cytogenetics becomes a robust framework for exploring biodiversity and chromosomal evolution within the complex Neotropical fauna. Despite these advances, substantial challenges remain, particularly the scarcity of cytogenetic data for many taxa and regions. Future research, driven by high-throughput genomic technologies, is expected to deepen our understanding of chromosomal evolution and to support conservation efforts for this rich but increasingly threatened fish fauna.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250190"},"PeriodicalIF":1.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13196785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNAs as regulators of chemoresistance and chemosensitivity in triple-negative breast cancer. lncrna作为三阴性乳腺癌化疗耐药和化疗敏感性的调节因子。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0242

Miguel Ángel Cáceres-Durán, Anna Carolina Lima Rodrigues, Juscelino Carvalho de Azevedo Junior, Caio Dantas Alves, Danielle Queiroz Calcagno, Bárbara do Nascimento Borges

{"title":"LncRNAs as regulators of chemoresistance and chemosensitivity in triple-negative breast cancer.","authors":"Miguel Ángel Cáceres-Durán, Anna Carolina Lima Rodrigues, Juscelino Carvalho de Azevedo Junior, Caio Dantas Alves, Danielle Queiroz Calcagno, Bárbara do Nascimento Borges","doi":"10.1590/1678-4685-GMB-2025-0242","DOIUrl":"10.1590/1678-4685-GMB-2025-0242","url":null,"abstract":"Triple-negative breast cancer (TNBC) is the most aggressive molecular subtype of breast cancer, characterized by significant heterogeneity and high recurrence rates. Due to this heterogeneity, TNBC often develops resistance to chemotherapy, leading to aggressive clinical behavior and poor prognosis. Long non-coding RNAs (lncRNAs), defined as RNAs longer than 500 nucleotides that act at both transcriptional and post-transcriptional levels, influence therapeutic responses in TNBC through various molecular mechanisms, thereby directly impacting treatment effectiveness and patient outcomes. Importantly, lncRNAs have a dual, context-dependent role in TNBC, either promoting chemoresistance or enhancing chemosensitivity by functioning as competing endogenous RNAs, regulating epigenetic processes, stabilizing mRNAs and proteins, and affecting DNA damage repair, apoptosis, autophagy, epithelial-mesenchymal transition, and pro-survival signaling pathways. These functions indicate their potential as therapeutic targets and biomarkers for personalized treatment. In this review, we examine the biological functions of lncRNAs in TNBC, highlighting their molecular mechanisms and clinical significance. We also review evidence on how lncRNAs contribute to therapeutic resistance and sensitivity and explore emerging perspectives on their potential as biomarkers and therapeutic targets for TNBC management.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250242"},"PeriodicalIF":1.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13196786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of reward-related genetic variants on BMI and predisposition to obesity: Systematic review and meta-analysis. 奖励相关基因变异对BMI和肥胖易感性的影响：系统回顾和荟萃分析。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0216

Bernardo Perin Cima, Mariana Meira Scudeler, Juliana Dal-Ri Lindenau, Yara Costa Netto Muniz

{"title":"Influence of reward-related genetic variants on BMI and predisposition to obesity: Systematic review and meta-analysis.","authors":"Bernardo Perin Cima, Mariana Meira Scudeler, Juliana Dal-Ri Lindenau, Yara Costa Netto Muniz","doi":"10.1590/1678-4685-GMB-2025-0216","DOIUrl":"10.1590/1678-4685-GMB-2025-0216","url":null,"abstract":"Obesity results from a chronic imbalance between caloric intake and energy expenditure, leading to the accumulation of excess body fat. This imbalance is influenced by endocrine, molecular, genetic, demographic, environmental, and lifestyle factors, with the brain reward cascade emerging as a key overlapping pathway. This study hypothesizes that cascade dysregulation leads to reward deficiency, causing stronger cravings and overeating. This study investigates the contribution of genetic variants in the brain reward cascade to body mass index (BMI), overweight, and obesity. A systematic search was conducted across five databases. Eligible studies evaluated associations between variants in reward-related genes (COMT, DRD2, DRD4, MAO-A, SLC6A3, SLC6A4) and BMI or obesity. In total, 117 studies were included in the systematic review, and 31 provided sufficient data for meta-analysis. Eighteen variants showed significant associations with BMI, with the strongest evidence observed for the Val158Met (COMT), Taq1A (DRD2), 48 bp VNTR (DRD4), 40 bp VNTR (SLC6A3) and 5-HTTLPR (SLC6A4). Meta-analyses of Taq1A showed a significant association of the A1 allele with obesity. This study highlights consistent associations between genetic variants in the reward cascade and susceptibility to weight gain, providing a foundation for more precise prevention and personalized obesity management.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250216"},"PeriodicalIF":1.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13196784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative synthesis of tandem repeats in the control region of Epinephelus mitogenomes (Peciformes: Epinephelidae). 石斑鱼丝裂体控制区串联重复序列的比较合成。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0240

Carla Bessa-Brito, Rodrigo Petry Corrêa de Sousa, Wanny Gomes de Lima, Renata Furtado do Rosário, Luan Pinto Rabelo, Yrlan Sousa Oliveira, Iracilda Sampaio, Grazielle Fernanda Evangelista Gomes, Marcelo Vallinoto

{"title":"Comparative synthesis of tandem repeats in the control region of Epinephelus mitogenomes (Peciformes: Epinephelidae).","authors":"Carla Bessa-Brito, Rodrigo Petry Corrêa de Sousa, Wanny Gomes de Lima, Renata Furtado do Rosário, Luan Pinto Rabelo, Yrlan Sousa Oliveira, Iracilda Sampaio, Grazielle Fernanda Evangelista Gomes, Marcelo Vallinoto","doi":"10.1590/1678-4685-GMB-2025-0240","DOIUrl":"10.1590/1678-4685-GMB-2025-0240","url":null,"abstract":"In this study, we analyzed the evolutionary patterns of tandem repeats in the mitochondrial Control Region (CR) of groupers of the genus Epinephelus. This research was based on a comprehensive survey of Epinephelus mitogenomes available in public databases, aiming to recover complete CR sequences. A total of 97 specimens were recovered, including 31 species that contain tandem repeat sequences. The repeated sequences were classified into three categories according to their length: short, medium, and long. The long repeats were restricted to a monophyletic group, while short and medium repeats were more widely distributed across different clades, whereas some species lacked repeats entirely. Given the similarities found among the repeat sequence alignments and the phylogenetic arrangements, it was possible to infer that these segments evolved in synchrony over time, with a process of concerted evolution being the most likely explanation for the evolutionary dynamics of these repetitive DNAs. Correlation analyses further revealed that mitogenome length covaries with CR length and that CR length increases with the full repeat region length, whereas motif length is inversely related to repeat copy number. These patterns underscore how repeat architecture evolves through time in the mitochondrial CR of epinephelid mitogenomes.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250240"},"PeriodicalIF":1.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13227324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular alterations in the GATA-2, RUNX1, C/EBPα and hTERT genes in patients with aplastic anemia by MLPA. MLPA对再生障碍性贫血患者GATA-2、RUNX1、C/EBPα和hTERT基因的影响

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0127

Iveth Mendoza Salas, Irma Olarte Carrillo, Rafael Cerón Maldonado, Anel Iraís García Laguna, Adrián De la Cruz Rosas, Christian Omar Ramos Peñafiel, Efreen Horacio Montaño Figueroa, Diana Laura Carrillo Rocha, Carlos Martínez Murillo, Verónica Fabiola Morán Barroso, Adolfo Martínez Tovar

{"title":"Molecular alterations in the GATA-2, RUNX1, C/EBPα and hTERT genes in patients with aplastic anemia by MLPA.","authors":"Iveth Mendoza Salas, Irma Olarte Carrillo, Rafael Cerón Maldonado, Anel Iraís García Laguna, Adrián De la Cruz Rosas, Christian Omar Ramos Peñafiel, Efreen Horacio Montaño Figueroa, Diana Laura Carrillo Rocha, Carlos Martínez Murillo, Verónica Fabiola Morán Barroso, Adolfo Martínez Tovar","doi":"10.1590/1678-4685-GMB-2025-0127","DOIUrl":"10.1590/1678-4685-GMB-2025-0127","url":null,"abstract":"Aplastic anemia (AA) is a disease characterized by a severe reduction of the erythroid lineage. Its molecular mechanisms have been studied using technologies such as whole-exome sequencing via NGS; however, this remains a costly and limited-access strategy for developing countries. In this study, 17 de novo patients diagnosed with AA were analyzed. Genomic DNA was isolated from each patient to perform the Multiplex Ligation-dependent Probe Amplification (MLPA) technique, which uses different probes to detect numerical alterations in the exons of the genes of interest (GATA2, RUNX1, C/EBPα, hTERT). In 70.9% cases, a molecular abnormality was found. GATA2 was the most frequently altered gene (58.8%), followed by TERT (47.0%), RUNX1 (41.1%), and finally C/EBPα (35.3%). Detecting these molecular alterations could help to understand the progression of AA to other hematologic malignancies due to the genomic instability associated with this panel of genes involved in various hematopoietic maturation processes.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 2","pages":"e20250127"},"PeriodicalIF":1.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13185482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147971902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoforms in action: The impact of transcript diversity in phenotype. 作用中的同工型：转录物多样性对表型的影响。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0220

Izabela Mamede, Carlos Mata-Machado, Clarisse Reis, Paulo P Amaral, Gloria R Franco

{"title":"Isoforms in action: The impact of transcript diversity in phenotype.","authors":"Izabela Mamede, Carlos Mata-Machado, Clarisse Reis, Paulo P Amaral, Gloria R Franco","doi":"10.1590/1678-4685-GMB-2025-0220","DOIUrl":"10.1590/1678-4685-GMB-2025-0220","url":null,"abstract":"The study of RNA processing, in particular how alternative splicing and the use of alternative transcription start and end sites generate diverse transcript isoforms from a single gene, has advanced significantly alongside the development of RNA sequencing technologies. High-resolution whole-transcriptome sequencing has shown that much of the transcriptomic diversity observed in humans, compared to other species, stems from these alternative RNA processing events. Primary studies first revealed that a single gene could give rise to multiple RNA variants, which challenges the linear interpretation of the central dogma of molecular biology. However, progress in the field was limited by technological constraints and the inherent complexity of resolving isoforms at high resolution. Recent breakthroughs, which includes long-read sequencing, high-throughput FISH-based imaging, isoform-specific quantification tools, and improved software for transcript detection and quantification, have reignited interest and enabled a more detailed view of transcriptomic diversity. Understanding alternative isoforms is significant, as they expand the proteome, fine-tune gene regulation, and influence cellular phenotypes that are key to human disease research and possible therapeutic development.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250220"},"PeriodicalIF":1.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13178736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic sequencing of Xanthomonas citri pathovars that cause diseases in tropical fruit and cotton plants. 在热带水果和棉花植物中引起疾病的柑橘黄单胞菌病原菌的基因组测序。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0079

Lucas Pontes de Lucena, Juan Carlos Ariute, Ana Maria Benko-Iseppon, Bertram Brenig, Vasco Azevedo, Flávia Figueira Aburjaile, Keyla Walescka Lopes da Silva, André da Silva Xavier, Elineide Barbosa de Souza, Marco Aurélio Siqueira da Gama

{"title":"Genomic sequencing of Xanthomonas citri pathovars that cause diseases in tropical fruit and cotton plants.","authors":"Lucas Pontes de Lucena, Juan Carlos Ariute, Ana Maria Benko-Iseppon, Bertram Brenig, Vasco Azevedo, Flávia Figueira Aburjaile, Keyla Walescka Lopes da Silva, André da Silva Xavier, Elineide Barbosa de Souza, Marco Aurélio Siqueira da Gama","doi":"10.1590/1678-4685-GMB-2025-0079","DOIUrl":"10.1590/1678-4685-GMB-2025-0079","url":null,"abstract":"Xanthomonas citri is a phytopathogenic species with a wide host range that harbors subspecies and pathovars such as citri, mangiferaeindicae, viticola, and malvacearum which cause citrus canker, bacterial canker of the mango, bacterial blight of cotton, and grapevine bacterial canker, respectively. In this work, 32 genomes belonging to these four pathovars were sequenced, two of which were from X. citri subsp. citri, one from X. citri pv. mangiferaeindicae, 13 from X. citri pv. viticola, and 16 from X. citri subsp. malvacearum. The genome size, N50, and GC content ranged from 4,978,419 to 5,256,019 bp, 67,322 to 727,867 bp, and 64.32% and 64.79%, respectively. All strains presented values of dDDH and ANIm greater than 77.3% and 96.1%, when compared to each other, and above 93% and 99%, when compared in relation to their respective pathotype strains. Maximum likelihood analysis of 1,298 core genes grouped the strains and their respective pathotype type with a 100% bootstrap. CRISPR sequences and genes from the Xop family (XopX, XopA, and XopV) and transcription activator-like effectors (TALE) were detected in all analyzed strains. The genomes sequenced in this study will help to understand the biology of these plant pathogens, enabling the development of more effective and precise management strategies and the prevention of disease outbreaks.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 1","pages":"e20250079"},"PeriodicalIF":1.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13178084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0