Combined expression of JHDM1D/KDM7A gene and long non-coding RNA RP11-363E7.4 as a biomarker for urothelial cancer prognosis.

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genetics and Molecular Biology Pub Date : 2024-08-09 eCollection Date: 2024-01-01 DOI:10.1590/1678-4685-GMB-2023-0265
Glenda Nicioli da Silva, Isadora Oliveira Ansaloni Pereira, Ana Paula Braga Lima, Tamires Cunha Almeida, André Luiz Ventura Sávio, Renato Prado Costa, Kátia Ramos Moreira Leite, Daisy Maria Fávero Salvadori
{"title":"Combined expression of JHDM1D/KDM7A gene and long non-coding RNA RP11-363E7.4 as a biomarker for urothelial cancer prognosis.","authors":"Glenda Nicioli da Silva, Isadora Oliveira Ansaloni Pereira, Ana Paula Braga Lima, Tamires Cunha Almeida, André Luiz Ventura Sávio, Renato Prado Costa, Kátia Ramos Moreira Leite, Daisy Maria Fávero Salvadori","doi":"10.1590/1678-4685-GMB-2023-0265","DOIUrl":null,"url":null,"abstract":"<p><p>Bladder cancer is the tenth most frequently diagnosed cancer globally. Classification of high- or low-grade tumors is based on cytological differentiation and is an important prognostic factor. LncRNAs regulate gene expression and play critical roles in the occurrence and development of cancer, however, there are few reports on their diagnostic value and co-expression levels with genes, which may be useful as specific biomarkers for prognosis and therapy in bladder cancer. Thus, we performed a marker lesion study to investigate whether gene/lncRNA expression in urothelial carcinoma tissues may be useful in differentiating low-grade and high-grade tumors. RT-qPCR was used to evaluate the expression of the JHDM1D gene and the lncRNAs CTD-2132N18.2, SBF2-AS1, RP11-977B10.2, CTD-2510F5.4, and RP11-363E7.4 in 20 histologically diagnosed high-grade and 10 low-grade tumors. A protein-to-protein interaction network between genes associated with JHDM1D gene was constructed using STRING website. The results showed a moderate (positive) correlation between CTD-2510F5.4 and CTD2132N18.2. ROC curve analyses showed that combined JHDM1D and RP11-363E7.4 predicted tumor grade with an AUC of 0.826, showing excellent accuracy. In conclusion, the results indicated that the combined expression of JHDM1D and RP11-363E7.4 may be a prognostic biomarker and a promising target for urothelial tumor therapy.</p>","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"47 3","pages":"e20230265"},"PeriodicalIF":1.7000,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320665/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetics and Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1590/1678-4685-GMB-2023-0265","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Bladder cancer is the tenth most frequently diagnosed cancer globally. Classification of high- or low-grade tumors is based on cytological differentiation and is an important prognostic factor. LncRNAs regulate gene expression and play critical roles in the occurrence and development of cancer, however, there are few reports on their diagnostic value and co-expression levels with genes, which may be useful as specific biomarkers for prognosis and therapy in bladder cancer. Thus, we performed a marker lesion study to investigate whether gene/lncRNA expression in urothelial carcinoma tissues may be useful in differentiating low-grade and high-grade tumors. RT-qPCR was used to evaluate the expression of the JHDM1D gene and the lncRNAs CTD-2132N18.2, SBF2-AS1, RP11-977B10.2, CTD-2510F5.4, and RP11-363E7.4 in 20 histologically diagnosed high-grade and 10 low-grade tumors. A protein-to-protein interaction network between genes associated with JHDM1D gene was constructed using STRING website. The results showed a moderate (positive) correlation between CTD-2510F5.4 and CTD2132N18.2. ROC curve analyses showed that combined JHDM1D and RP11-363E7.4 predicted tumor grade with an AUC of 0.826, showing excellent accuracy. In conclusion, the results indicated that the combined expression of JHDM1D and RP11-363E7.4 may be a prognostic biomarker and a promising target for urothelial tumor therapy.

JHDM1D/KDM7A基因和长非编码RNA RP11-363E7.4的联合表达可作为尿路癌预后的生物标记。
膀胱癌是全球第十大最常见的癌症。高分化或低分化肿瘤的分类基于细胞学分化,是重要的预后因素。LncRNAs 可调控基因表达,在癌症的发生和发展过程中起着至关重要的作用,然而,关于其诊断价值以及与基因的共表达水平的报道却很少,而这可能是膀胱癌预后和治疗的特异性生物标志物。因此,我们进行了一项标记病变研究,以探讨尿路上皮癌组织中基因/lncRNA的表达是否有助于区分低级别和高级别肿瘤。采用 RT-qPCR 评估了 20 例组织学诊断为高级别和 10 例低级别的肿瘤中 JHDM1D 基因和 lncRNA CTD-2132N18.2、SBF2-AS1、RP11-977B10.2、CTD-2510F5.4 和 RP11-363E7.4 的表达。利用 STRING 网站构建了与 JHDM1D 基因相关的基因间的蛋白-蛋白相互作用网络。结果显示,CTD-2510F5.4 和 CTD2132N18.2 之间存在中度(正)相关性。ROC曲线分析表明,JHDM1D和RP11-363E7.4联合预测肿瘤分级的AUC为0.826,显示了极高的准确性。总之,研究结果表明,JHDM1D和RP11-363E7.4的联合表达可能是一种预后生物标志物,也是一种很有前景的尿路肿瘤治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genetics and Molecular Biology
Genetics and Molecular Biology 生物-生化与分子生物学
CiteScore
4.20
自引率
4.80%
发文量
111
审稿时长
3 months
期刊介绍: Genetics and Molecular Biology (formerly named Revista Brasileira de Genética/Brazilian Journal of Genetics - ISSN 0100-8455) is published by the Sociedade Brasileira de Genética (Brazilian Society of Genetics). The Journal considers contributions that present the results of original research in genetics, evolution and related scientific disciplines. Manuscripts presenting methods and applications only, without an analysis of genetic data, will not be considered.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信