Genetics and Molecular Biology最新文献_第4页

Partial molecular characterization, expression pattern and polymorphism analysis of MHC I genes in Chinese domestic goose (Anser cygnoides). 中国家鹅（Anser cygnoides）MHC I 基因的部分分子特征、表达模式和多态性分析。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2024-07-08 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2022-0252

Qianqian Zeng, Xiaojie Li, Xiaomin Shi, Shigan Yan

{"title":"Partial molecular characterization, expression pattern and polymorphism analysis of MHC I genes in Chinese domestic goose (Anser cygnoides).","authors":"Qianqian Zeng, Xiaojie Li, Xiaomin Shi, Shigan Yan","doi":"10.1590/1678-4685-GMB-2022-0252","DOIUrl":"10.1590/1678-4685-GMB-2022-0252","url":null,"abstract":"Major histocompatibility complex (MHC) allelic polymorphism is critically important for mediating antigen presentation in vertebrates. Presently, there are insufficient studies of MHC genetic diversity in domestic Anseriform birds. In this study, we analyzed the expression profile of MHC I genes and screened for MHC I exon 2 polymorphism in one domestic goose population from China using Illumina MiSeq sequencing. The results showed that four MHC I alleles (Ancy-IE2*09/*11/*13/*21) in one goose were identified based on cDNA cloning and sequencing using four primer combinations, and the varying number of cDNA clones implied that these four classical sequences showed differential expression patterns. Through next-generation sequencing, 27 alleles were obtained from 68 geese with 3-10 putative alleles per individual, indicating at least the existence of 5 MHC I loci in the goose. The marked excess of the non-synonymous over the synonymous substitution in the peptide-binding region (PBR) along 27 alleles and five positively selected sites (PSSs) detected around the PBR indicated that balancing selection might be the major force in shaping high MHC variation in the goose. Additionally, IA alleles displaying lower polymorphism were subject to less positive selection pressure than non-IA alleles with a higher level of polymorphism.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring mood disorders and treatment options using human stem cells. 利用人类干细胞探索情绪障碍和治疗方案。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0305

Autumn Hudock, Zaira Paulina Leal, Amandeep Sharma, Arianna Mei, Renata Santos, Maria Carolina Marchetto

{"title":"Exploring mood disorders and treatment options using human stem cells.","authors":"Autumn Hudock, Zaira Paulina Leal, Amandeep Sharma, Arianna Mei, Renata Santos, Maria Carolina Marchetto","doi":"10.1590/1678-4685-GMB-2023-0305","DOIUrl":"10.1590/1678-4685-GMB-2023-0305","url":null,"abstract":"Despite their global prevalence, the mechanisms for mood disorders like bipolar disorder and major depressive disorder remain largely misunderstood. Mood stabilizers and antidepressants, although useful and effective for some, do not have a high responsiveness rate across those with these conditions. One reason for low responsiveness to these drugs is patient heterogeneity, meaning there is diversity in patient characteristics relating to genetics, etiology, and environment affecting treatment. In the past two decades, novel induced pluripotent stem cell (iPSC) research and technology have enabled the use of human-derived brain cells as a new model to study human disease that can help account for patient variance. Human iPSC technology is an emerging tool to better understand the molecular mechanisms of these disorders as well as a platform to test novel treatments and existing pharmaceuticals. This literature review describes the use of iPSC technology to model bipolar and major depressive disorder, common medications used to treat these disorders, and novel patient-derived alternative treatment methods for non-responders stemming from past publications, as well as presenting new data derived from these models.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ΔNp63α promotes cigarette smoke-induced renal cancer stem cell activity via the Sonic Hedgehog pathway. ΔNp63α通过Sonic Hedgehog途径促进香烟烟雾诱导的肾癌干细胞活性

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0347

Yuxiang Zhao, Nannan Ma, Wanngyu Wu, Ying Wu, Wenbo Zhang, Weiwei Qian, Xin Sun, Tao Zhang

{"title":"ΔNp63α promotes cigarette smoke-induced renal cancer stem cell activity via the Sonic Hedgehog pathway.","authors":"Yuxiang Zhao, Nannan Ma, Wanngyu Wu, Ying Wu, Wenbo Zhang, Weiwei Qian, Xin Sun, Tao Zhang","doi":"10.1590/1678-4685-GMB-2023-0347","DOIUrl":"10.1590/1678-4685-GMB-2023-0347","url":null,"abstract":"Cigarette smoke (CS) has been generally recognized as a chief carcinogenic factor in renal cell carcinoma (RCC). The stimulative effect of CS on renal cancer stem cells (RCSCs) has been described previously. The Sonic Hedgehog (SHH) pathway plays an essential role in self-renewal, cell growth, drug resistance, metastasis, and recurrence of cancer stem cells (CSCs). Renal cancer-related gene ΔNp63α is highly expressed in renal epithelial tissues and contributes to the RCSCs characteristics of tumors. The aim of this study was to elucidate the role of ΔNp63α and the SHH pathway on the activity of RCSCs induced by CS through a series of in vivo and in vitro studies. It was shown that in renal cancer tissues, ΔNp63α and RCSCs markers in smokers are expressed higher than that in non-smokers. RCSCs were effectively enriched by tumor sphere formation assay. Besides, CS increased the expression of RCSCs markers and the capability of sphere-forming in vitro and in vivo. Moreover, the SHH pathway was activated, and the specialized inhibitor alleviated the promotion of CS on RCSCs. ΔNp63α activated the SHH pathway and promoted CS-induced enhancement of RCSCs activity. These findings indicate that ΔNp63α positively regulates the activity of CS-induced RCSCs via the SHH pathway.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mitogenomic landscape of Banisteriopsis caapi (Malpighiaceae), the sacred liana used for ayahuasca preparation. 用于制作死藤水的神草 Banisteriopsis caapi（Malpighiaceae）的有丝分裂基因组图谱。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0301

Edisson Chavarro-Mesa, João Victor Dos Anjos Almeida, Saura R Silva, Simone Santos Lopes, Jose Beethoven Figueiredo Barbosa, Danilo Oliveira, Maria Alice Corrêa, Ana Paula Moraes, Vitor F O Miranda, Francisco Prosdocimi, Alessandro M Varani

{"title":"The mitogenomic landscape of Banisteriopsis caapi (Malpighiaceae), the sacred liana used for ayahuasca preparation.","authors":"Edisson Chavarro-Mesa, João Victor Dos Anjos Almeida, Saura R Silva, Simone Santos Lopes, Jose Beethoven Figueiredo Barbosa, Danilo Oliveira, Maria Alice Corrêa, Ana Paula Moraes, Vitor F O Miranda, Francisco Prosdocimi, Alessandro M Varani","doi":"10.1590/1678-4685-GMB-2023-0301","DOIUrl":"10.1590/1678-4685-GMB-2023-0301","url":null,"abstract":"The sacred ayahuasca brew, utilized by indigenous communities in the Amazon and syncretic religious groups in Brazil, primarily consists of a decoction of two plants: (i) the Amazonian liana known as Mariri or Jagube (Banisteriopsis caapi), and (ii) the shrub referred as Chacrona or Rainha (Psychotria viridis). While Chacrona leaves are rich in N,N-Dimethyltryptamine (DMT), a potent psychedelic, the macerated vine of Mariri provides beta-carboline alkaloids acting as monoamine oxidase inhibitors, preventing DMT's degradation. This study sequenced, assembled, and analyzed the complete genome of B. caapi's mitochondrion, yielding a circular structure spanning 503,502 bp. Although the mtDNA encompasses most plant mitochondrial genes, it lacks some ribosomal genes, presents some atypical genes, and contains plastid pseudogenes, suggesting gene transfer between organelles. The presence of a 7-Kb repetitive segment containing copies of the rrnL and trnfM genes suggests mitogenome isomerization, supporting the hypothesis of dynamic mitogenome maintenance in plants. Phylogenetics and phylogenomics across 24 Malpighiales confirms the sample's placement in the \"Tucunacá\" ethnovariety, aligning with morphological identification. This study spearheads efforts to decode the genome of this esteemed Malpighiaceae.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Legume-rhizobia symbiosis: Translatome analysis. 豆科植物与根瘤菌的共生关系：翻译组分析。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0284

María Martha Sainz, Mariana Sotelo-Silveira, Carla V Filippi, Sofía Zardo

{"title":"Legume-rhizobia symbiosis: Translatome analysis.","authors":"María Martha Sainz, Mariana Sotelo-Silveira, Carla V Filippi, Sofía Zardo","doi":"10.1590/1678-4685-GMB-2023-0284","DOIUrl":"10.1590/1678-4685-GMB-2023-0284","url":null,"abstract":"Leguminous plants can establish endosymbiotic relationships with nitrogen-fixing soil rhizobacteria. Bacterial infection and nodule organogenesis are two independent but highly coordinated genetic programs that are active during this interaction. These genetic programs can be regulated along all the stages of gene expression. Most of the studies, for both eukaryotes and prokaryotes, focused on the transcriptional regulation level determining the abundance of mRNAs. However, it has been demonstrated that mRNA levels only sometimes correlate with the abundance or activity of the coded proteins. For this reason, in the past two decades, interest in the role of translational control of gene expression has increased, since the subset of mRNA being actively translated outperforms the information gained only by the transcriptome. In the case of legume-rhizobia interactions, the study of the translatome still needs to be explored further. Therefore, this review aims to discuss the methodologies for analyzing polysome-associated mRNAs at the genome-scale and their contribution to studying translational control to understand the complexity of this symbiotic interaction. Moreover, the Dual RNA-seq approach is discussed for its relevance in the context of a symbiotic nodule, where intricate multi-species gene expression networks occur.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the chemoreceptor repertoire of a highly specialized fly with comparisons to other Drosophila species. 一种高度特化的蝇类化学感受器的特性以及与其他果蝇物种的比较。

IF 2.1 4区生物学

Genetics and Molecular Biology Pub Date : 2024-06-14 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2022-0383

Pedro Mesquita Fonseca, Lizandra Jaqueline Robe, Tuane Letícia Carvalho, Elgion Lucio Silva Loreto

{"title":"Characterization of the chemoreceptor repertoire of a highly specialized fly with comparisons to other Drosophila species.","authors":"Pedro Mesquita Fonseca, Lizandra Jaqueline Robe, Tuane Letícia Carvalho, Elgion Lucio Silva Loreto","doi":"10.1590/1678-4685-GMB-2022-0383","DOIUrl":"https://doi.org/10.1590/1678-4685-GMB-2022-0383","url":null,"abstract":"To explore the diversity of scenarios in nature, animals have evolved tools to interact with different environmental conditions. Chemoreceptors are an important interface component and among them, olfactory receptors (ORs) and gustatory receptors (GRs) can be used to find food and detect healthy resources. Drosophila is a model organism in many scientific fields, in part due to the diversity of species and niches they occupy. The contrast between generalists and specialists Drosophila species provides an important model for studying the evolution of chemoreception. Here, we compare the repertoire of chemoreceptors of different species of Drosophila with that of D. incompta, a highly specialized species whose ecology is restricted to Cestrum flowers, after reporting the preferences of D. incompta to the odor of Cestrum flowers in olfactory tests. We found evidence that the chemoreceptor repertoire in D. incompta is smaller than that presented by species in the Sophophora subgenus. Similar patterns were found in other non-Sophophora species, suggesting the presence of underlying phylogenetic trends. Nevertheless, we also found autapomorphic gene losses and detected some genes that appear to be under positive selection in D. incompta, suggesting that the specific lifestyle of these flies may have shaped the evolution of individual genes in each of these gene families.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11182316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete chloroplast genome of Dicliptera tinctoria (Nees) Kostel. and comparative analysis of chloroplast genomes in Acanthaceae. Dicliptera tinctoria (Nees) Kostel.的完整叶绿体基因组以及刺桐科植物叶绿体基因组的比较分析。

IF 2.1 4区生物学

Genetics and Molecular Biology Pub Date : 2024-06-14 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0297

Thi Thanh Nga Le, Minh Thiet Vu, Hoang Dang Khoa Do

{"title":"The complete chloroplast genome of Dicliptera tinctoria (Nees) Kostel. and comparative analysis of chloroplast genomes in Acanthaceae.","authors":"Thi Thanh Nga Le, Minh Thiet Vu, Hoang Dang Khoa Do","doi":"10.1590/1678-4685-GMB-2023-0297","DOIUrl":"https://doi.org/10.1590/1678-4685-GMB-2023-0297","url":null,"abstract":"Dicliptera tinctoria is a member of Acanthaceae, which has a wide distribution and contains potentially medicinal species, and exhibited pharmaceutical potentials. This study sequenced and characterized the complete chloroplast genome of Dicliptera tinctoria. The newly sequenced cpDNA of D. tinctoria was 150,733 bp in length and had a typical quadripartite structure consisting of a large single copy (LSC, 82,895 bp), a small single copy (SSC, 17,249 bp), and two inverted repeat (IRs, 25,295 bp each) regions. This genome also contained 80 protein-coding genes, 30 transfer RNAs, and four ribosomal RNAs, which is identical to other chloroplast genomes in Acanthaceae family. Nucleotides diversity analysis among chloroplast genomes of Acanthaceae species revealed eight hypervariable regions, including trnK_UUU-matK, trnC_GCA-petN, accD, rps12-clpP, rps3-rps19, ycf1-ndhF, ccsA-ndhD, and ycf1. Phylogenetic analysis revealed the paraphyly of Dicliptera species and monophyly in four Acanthaceae subfamilies. These results provide an overview of genomic variations in Acanthaceae chloroplast genome, which is helpful for further genomic studies.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11182309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the role of Peg13: A promising therapeutic target for mitigating inflammation in sepsis. 揭示 Peg13 的作用：缓解败血症炎症的有望治疗靶点。

IF 2.1 4区生物学

Genetics and Molecular Biology Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0205

Dan Wang, Zhiqiang Lin, Meixia Su, Yiqing Zhou, Mengjie Ma, Minghui Li

{"title":"Revealing the role of Peg13: A promising therapeutic target for mitigating inflammation in sepsis.","authors":"Dan Wang, Zhiqiang Lin, Meixia Su, Yiqing Zhou, Mengjie Ma, Minghui Li","doi":"10.1590/1678-4685-GMB-2023-0205","DOIUrl":"10.1590/1678-4685-GMB-2023-0205","url":null,"abstract":"To investigate the role of Peg13 in modulating the inflammatory response in sepsis, we established Lipopolysaccharide (LPS)-induced 293T cells and mouse models. Peg13 expression was assessed at various time points after infection using RT-qPCR. The levels of high mobility group box 1 (HMGB1) and interleukin-6 (IL-6) were quantified through ELISA. A total of 44 septic patients and 36 healthy participants were recruited to measure Peg13 and HMGB1 levels in the blood. Peg13 demonstrated significant down-regulation in the supernatant of LPS-induced 293T cells and in the blood of LPS-induced mice. Moreover, the levels of proinflammatory cytokines HMGB1 and IL-6 were elevated in both the supernatant of LPS-induced cell models and blood specimens from LPS-induced murine models, and this elevation could be notably reduced by Peg13 suppression. In a clinical context, Peg13 and HMGB1 levels were higher in septic patients compared to healthy subjects. Peg13 exhibited a negative correlation with HMGB1, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) among septic patients. Peg13 mitigates the inflammatory response by reducing the release of proinflammatory cytokines HMGB1 and IL-6 in sepsis, presenting a potential therapeutic target for alleviating inflammation in sepsis treatment.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of chaperone-mediated autophagy in drug resistance. 伴侣介导的自噬在耐药性中的作用

IF 2.1 4区生物学

Genetics and Molecular Biology Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0317

Ana Beatriz da Silva Teixeira, Maria Carolina Clares Ramalho, Izadora de Souza, Izabela Amélia Marques de Andrade, Isabeli Yumi Araújo Osawa, Camila Banca Guedes, Beatriz Silva de Oliveira, Cláudio Henrique Dahne de Souza Filho, Tainá Lins da Silva, Natália Cestari Moreno, Marcela Teatin Latancia, Clarissa Ribeiro Reily Rocha

{"title":"The role of chaperone-mediated autophagy in drug resistance.","authors":"Ana Beatriz da Silva Teixeira, Maria Carolina Clares Ramalho, Izadora de Souza, Izabela Amélia Marques de Andrade, Isabeli Yumi Araújo Osawa, Camila Banca Guedes, Beatriz Silva de Oliveira, Cláudio Henrique Dahne de Souza Filho, Tainá Lins da Silva, Natália Cestari Moreno, Marcela Teatin Latancia, Clarissa Ribeiro Reily Rocha","doi":"10.1590/1678-4685-GMB-2023-0317","DOIUrl":"10.1590/1678-4685-GMB-2023-0317","url":null,"abstract":"In the search for alternatives to overcome the challenge imposed by drug resistance development in cancer treatment, the modulation of autophagy has emerged as a promising alternative that has achieved good results in clinical trials. Nevertheless, most of these studies have overlooked a novel and selective type of autophagy: chaperone-mediated autophagy (CMA). Following its discovery, research into CMA's contribution to tumor progression has accelerated rapidly. Therefore, we now understand that stress conditions are the primary signal responsible for modulating CMA in cancer cells. In turn, the degradation of proteins by CMA can offer important advantages for tumorigenesis, since tumor suppressor proteins are CMA targets. Such mutual interaction between the tumor microenvironment and CMA also plays a crucial part in establishing therapy resistance, making this discussion the focus of the present review. Thus, we highlight how suppression of LAMP2A can enhance the sensitivity of cancer cells to several drugs, just as downregulation of CMA activity can lead to resistance in certain cases. Given this panorama, it is important to identify selective modulators of CMA to enhance the therapeutic response.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11145944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of tumor plasticity by senescent cells: Deciphering basic mechanisms and survival pathways to unravel therapeutic options. 衰老细胞对肿瘤可塑性的调节：解密基本机制和生存途径，探索治疗方案。

IF 2.1 4区生物学

Genetics and Molecular Biology Pub Date : 2024-05-27 eCollection Date: 2024-01-01 DOI: 10.1590/1678-4685-GMB-2023-0311

Andrew Oliveira Silva, Thais Cardoso Bitencourt, Jose Eduardo Vargas, Lucas Rosa Fraga, Eduardo Filippi-Chiela

{"title":"Modulation of tumor plasticity by senescent cells: Deciphering basic mechanisms and survival pathways to unravel therapeutic options.","authors":"Andrew Oliveira Silva, Thais Cardoso Bitencourt, Jose Eduardo Vargas, Lucas Rosa Fraga, Eduardo Filippi-Chiela","doi":"10.1590/1678-4685-GMB-2023-0311","DOIUrl":"10.1590/1678-4685-GMB-2023-0311","url":null,"abstract":"Senescence is a cellular state in which the cell loses its proliferative capacity, often irreversibly. Physiologically, it occurs due to a limited capacity of cell division associated with telomere shortening, the so-called replicative senescence. It can also be induced early due to DNA damage, oncogenic activation, oxidative stress, or damage to other cellular components (collectively named induced senescence). Tumor cells acquire the ability to bypass replicative senescence, thus ensuring the replicative immortality, a hallmark of cancer. Many anti-cancer therapies, however, can lead tumor cells to induced senescence. Initially, this response leads to a slowdown in tumor growth. However, the longstanding accumulation of senescent cells (SnCs) in tumors can promote neoplastic progression due to the enrichment of numerous molecules and extracellular vesicles that constitutes the senescence-associated secretory phenotype (SASP). Among other effects, SASP can potentiate or unlock the tumor plasticity and phenotypic transitions, another hallmark of cancer. This review discusses how SnCs can fuel mechanisms that underlie cancer plasticity, like cell differentiation, stemness, reprogramming, and epithelial-mesenchymal transition. We also discuss the main molecular mechanisms that make SnCs resistant to cell death, and potential strategies to target SnCs. At the end, we raise open questions and clinically relevant perspectives in the field.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11132560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0