General physiology and biophysics最新文献_第7页

Immunobiochemical profile of clear cell renal cell carcinoma (ccRCC): A preliminary study. 透明细胞肾细胞癌(ccRCC)的免疫生化特征:初步研究。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023015

Daniela Vargová, Ján Dargaj, Soňa Fraňová, Matúš Dohál, Ján Ľupták, Ján Švihra, Lukáš Briš, Marián Grendár, Martina Šutovská

{"title":"Immunobiochemical profile of clear cell renal cell carcinoma (ccRCC): A preliminary study.","authors":"Daniela Vargová, Ján Dargaj, Soňa Fraňová, Matúš Dohál, Ján Ľupták, Ján Švihra, Lukáš Briš, Marián Grendár, Martina Šutovská","doi":"10.4149/gpb_2023015","DOIUrl":"https://doi.org/10.4149/gpb_2023015","url":null,"abstract":"Clear cell renal cell carcinoma (ccRCC) is the most common variant of RCC. It is an aggressive disease with an unfavorable prognosis. The rich immune infiltrates present in the tumor microenvironment (TME) of ccRCC produce various signaling molecules, especially cytokines, which primarily activate the Jak/STAT pathway and significantly influence tumor pathogenesis. STAT3 has a well-defined oncogenic character. Using multiplex assays and ELISA, we have measured the concentrations of 27 cytokines and STAT3 in tumor and healthy renal tissue from 16 patients with histologically verified ccRCC. We have detected significantly higher levels of G-CSF, IL-6, CXCL10, CCL3, and CCL4 in tumor tissue than in their healthy counterparts. There were significant differences in the levels of IL-1β and PDGF-BB between tumors of different nuclear grades (NG). Intratumoral IL-12p70 and IL-15 showed a significant positive correlation with intratumoral STAT3. The concentration of STAT3 in tumors was significantly lower than in the kidney. An increase in tumor STAT3 levels was associated with an increase in the pathological stage of the disease (TNM), but not with NG. The results of our study confirm the significant role of various cytokines and STAT3 in the pathogenesis of ccRCC and indicate their clinical relevance.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 5","pages":"387-401"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and prognostic significance of CCPG1 in hepatocellular carcinoma. CCPG1在肝癌中的临床及预后意义。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023017

Gangpu Wang, Zhiqian Liu, Qi Wang, Bingqi Liu, Jie Li

{"title":"Clinical and prognostic significance of CCPG1 in hepatocellular carcinoma.","authors":"Gangpu Wang, Zhiqian Liu, Qi Wang, Bingqi Liu, Jie Li","doi":"10.4149/gpb_2023017","DOIUrl":"https://doi.org/10.4149/gpb_2023017","url":null,"abstract":"This study aimed to examine the clinical and prognostic significance of cell-cycle progression gene 1 (CCPG1) in hepatocellular carcinoma (HCC). We firstly analyzed CCPG1 expression in various cancers using The Cancer Genome Atlas and the Genotype-Tissue Expression project databases. The relative expression levels of CCPG1 were determined in 164 paired HCC and adjacent tissues using immunohistochemistry. The correlation between CCPG1 and clinicopathological characteristics of HCC was analyzed. Cox proportional models were used to identify the prognostic factors for overall survival (OS) and disease-free survival (DFS). The expression of CCPG1 was lower in HCC tissues than in adjacent non-tumor liver tissues. The expression of CCPG1 was significantly correlated with tumor number (p = 0.02) and tumor differentiation (p = 0.04) in HCC. Lower expression of CCPG1 in HCC patients was associated with poor OS and DFS (p < 0.01). Relative low expression of CCPG1 in HCC is significantly correlated with the poor prognosis of HCC patients after surgical resection, suggesting its possible role as a potential prognostic marker for HCC.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 5","pages":"431-442"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10259497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of quercetin and its derivatives with Ca2+-ATPase from sarcoplasmic reticulum: Kinetic and molecular modeling studies. 槲皮素及其衍生物与肌浆网Ca2+- atp酶的相互作用:动力学和分子模型研究。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023020

Petronela Rezbarikova, Jana Viskupicova, Magdalena Majekova, Lubica Horakova

引用次数: 0

Correlation of microsatellite status and EBV infection with clinical characteristics of patients with gastric cancer. 微卫星状态和EBV感染与胃癌患者临床特征的相关性研究。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023021

Pengfei Lu, Kadeliya Yushan, Gulimilai Yusufu, Hiu Wang, Rui Mao

{"title":"Correlation of microsatellite status and EBV infection with clinical characteristics of patients with gastric cancer.","authors":"Pengfei Lu, Kadeliya Yushan, Gulimilai Yusufu, Hiu Wang, Rui Mao","doi":"10.4149/gpb_2023021","DOIUrl":"https://doi.org/10.4149/gpb_2023021","url":null,"abstract":"This study was designed to investigate the correlation of microsatellite status (MS) and Epstein-Barr virus (EBV) infection with the clinical characteristics of gastric cancer (GC) patients. MS was detected by immunohistochemistry. EBV was detected by in situ hybridization. There were 31.3% cases showed mismatch repair-deficient (dMMR)/ microsatellite instability (MSI) and 68.7% cases showed mismatch repair-proficient (pMMR)/ microsatellite stability (MSS). The dMMR/MSI was more common in the elderly, in patients with cardia GC, smaller tumor diameter or non-poorly differentiated carcinoma. The survival in dMMR/MSI patients tended to be longer than that in pMMR/MSS patients. Total 7.6% cases showed EBV-positive (EBV(+)) among 198 GC patients. EBV(+) was more common in patients with advanced GC or poorly differentiated adenocarcinoma. MSI was more common in EBV-negative (EBV(-)) patients than in EBV(+) patients. The dMMR/MSI patients with stage II GC benefited from chemotherapy. The survival of EBV(+) patients tended to be longer than that of EBV(-) patients.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 5","pages":"403-415"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA XIST promotes insulin resistance in gestational diabetes mellitus via the microRNA-181b-5p/NDRG2 axis. LncRNA XIST通过microRNA-181b-5p/NDRG2轴促进妊娠期糖尿病胰岛素抵抗。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023019

Yanli Xu, Xiaodi Kang, Huafang Liu, Hongli Jiang, Wenjing Wang

{"title":"LncRNA XIST promotes insulin resistance in gestational diabetes mellitus via the microRNA-181b-5p/NDRG2 axis.","authors":"Yanli Xu, Xiaodi Kang, Huafang Liu, Hongli Jiang, Wenjing Wang","doi":"10.4149/gpb_2023019","DOIUrl":"https://doi.org/10.4149/gpb_2023019","url":null,"abstract":"Many studies have explored the role of lncRNA X inactivation-specific transcript (XIST) in diabetes. This study was designed to unravel the regulatory mechanism of XIST on animal models of gestational diabetes mellitus (GDM) progression via the microRNA (miR)-181b-5p/N-myc downstream-regulated gene 2 (NDRG2) axis. XIST, miR-181b-5p, and NDRG2 expression levels in GDM mice were detected. The GDM mice were subjected to gain- and loss-of-function assays to examine the change of glucose metabolism indices (fasting blood glucose (FBG), fasting insulin (FINS) and homeostasis model assessment of insulin resistance (HOMA-IR)), serum oxidative stress factors (glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA)), serum inflammatory factors (interleukin-1 β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α)), pathological changes of pancreatic tissues, and apoptotic cells in pancreatic islets in GDM mice. XIST and NDRG2 expression were elevated while miR-181b-5p expression was depleted in GDM mice. Down-regulated XIST or NDRG2 or up-regulated miR-181b-5p reduced the FBG level, HOMA-IR, and serum IL-1β, IL-6, and TNF-α, and MDA contents, elevated the FINS, GSH, and SOD level, mitigated pathological changes in pancreatic tissues, and decelerated apoptotic cells in pancreatic islets in GDM mice. Silenced XIST dampens insulin resistance in GDM mice via the modulation of the miR-181b-5p/NDRG2 axis.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 5","pages":"443-455"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DNMT1-induced miR-133b suppression via methylation promotes myocardial fibrosis after myocardial infarction. dnmt1通过甲基化诱导的miR-133b抑制促进心肌梗死后心肌纤维化。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-09-01 DOI: 10.4149/gpb_2023018

Songlin Zhang, Hang Xie, Yajuan Du, Boxiang Wang, Beidi Lan, Haiyan Wang

{"title":"DNMT1-induced miR-133b suppression via methylation promotes myocardial fibrosis after myocardial infarction.","authors":"Songlin Zhang, Hang Xie, Yajuan Du, Boxiang Wang, Beidi Lan, Haiyan Wang","doi":"10.4149/gpb_2023018","DOIUrl":"https://doi.org/10.4149/gpb_2023018","url":null,"abstract":"Myocardial fibrosis is an underlying cause of many cardiovascular diseases. Novel insights into the epigenetic control of myocardial fibrosis are now emerging. The current work is focused on investigating the biological role of DNA methyltransferase 1 (DNMT1) in myocardial fibrosis as well as the underlying mechanism. Our findings revealed that DNMT1 expression levels were upregulated, whereas miR-133b expression levels were decreased in a rat model of myocardial fibrosis following myocardial infarction. In vitro, the expression levels of DNMT1 increased and those of miR-133b decreased after Ang-II treatment in cardiac fibroblasts. DNMT1 knockdown inhibited Ang-II-induced cardiac myofibroblast activation, and DNMT1 overexpression increased the proliferation and collagen generation of cardiac myofibroblasts. Furthermore, DNMT1 expression levels decreased, while miR-133b expression levels increased after treatment with 5-Aza (5-Azacytidine, a known inhibitor of DNA methylation) in Ang-II-induced cardiac fibroblasts. BSP (Bisulfite sequencing PCR) results showed a marked decrease in methylation levels in the miR-133b promoter region upon overexpression of DNMT1, whereas knockdown of DNMT1 blocked increased methylation levels in the miR-133b promoter region in Ang-II-induced cardiac fibroblasts. Finally, 5-Aza treatment reduced the progression of myocardial fibrosis after myocardial infarction in rats in vivo. Collectively, our results suggest that DNMT1 mediates CTGF expression in cardiac fibroblast activation by regulating the methylation of miR-133b. The present work reveals the unique role of the DNMT1/miR-133b/CTGF axis in myocardial fibrosis, thus suggesting its great therapeutic potential in the treatment of cardiac diseases.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 5","pages":"417-429"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10259505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The morphological and functional ultrastructure of cells in pre-implantation embryos. 着床前胚胎细胞的形态和功能超微结构。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-07-01 DOI: 10.4149/gpb_2023011

Juraj Pivko, Alexander Makarevich, Lucia Olexiková, Elena Kubovičová, Peter Makovický, Linda Dujičková, Jiří Bezdíček

{"title":"The morphological and functional ultrastructure of cells in pre-implantation embryos.","authors":"Juraj Pivko, Alexander Makarevich, Lucia Olexiková, Elena Kubovičová, Peter Makovický, Linda Dujičková, Jiří Bezdíček","doi":"10.4149/gpb_2023011","DOIUrl":"https://doi.org/10.4149/gpb_2023011","url":null,"abstract":"Cells of pre-implantation embryos are equipped with many morphological and functional systems through which they can synthesize specific proteins and effectively ensure the protection of early embryonic development. Here we present evidence for the existence of these systems in morphologically normal and abnormal bovine blastocyst stage embryos in vivo at the ultrastructural and actin cytoskeleton levels. The appearance of organelles in the trophectoderm (TE) and inner cell mass (ICM) cells, responsible for their synthetic activities and their role in the development of early bovine embryos are described. We point out the importance of endocytic processes and the participation of extracellular vesicles in the formation of intercellular contacts and homeostasis of the embryo microenvironment. Several changes in the ultrastructural morphology of embryos produced by different methods (ICSI, parthenogenetic AC/DC electrical activation, IVF with separated sperm) and freezing/thawed embryos are described. We also show alterations occurred in the organelles after viral contamination of embryos with BHV-1 and BVDV viruses, and in embryos from over-conditioned cows. Recorded changes in organelles and appearance of cellular autophagic structures (vesicles, multivesicular bodies and autophagolysosomes) may negatively affect embryo metabolism and lead to the emergence of pathological processes in TE and ICM cells of preimplantation embryos.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 4","pages":"307-321"},"PeriodicalIF":1.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomedical research brings mTBI biomarkers a step closer to the bedside - implementation in clinical practice. 生物医学研究使mTBI生物标志物在临床实践中更接近床边实施。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-07-01 DOI: 10.4149/gpb_2023013

Martina Martinikova, Robert Ruzinak, Egon Kurca, Michael Teodor Grey, Stefan Sivak

{"title":"Biomedical research brings mTBI biomarkers a step closer to the bedside - implementation in clinical practice.","authors":"Martina Martinikova, Robert Ruzinak, Egon Kurca, Michael Teodor Grey, Stefan Sivak","doi":"10.4149/gpb_2023013","DOIUrl":"https://doi.org/10.4149/gpb_2023013","url":null,"abstract":"Research in the field of TBI (traumatic brain injury) has long been focused on severe brain injury, while the number of mild injuries far overweigh severe injuries. Mild head injuries constitute up to 95% of all traumatic head injuries. The purpose of this work is to identify mTBI (mild traumatic brain injury) patients who are unlikely to benefit from CT (computed tomography) scanning. Biomarkers capable of clearly discriminating between CT-positive and CT-negative subjects are needed. Biomarkers hold the potential to document whether a concussion occurred, especially when the history is unclear and neurocognitive sequelae persist. Recently, following advances in proteomics analysis, investigators have introduced ubiquitin C-terminal hydrolase-L1 (UCH-L1) and glial fibrillary acidic protein (GFAP) as two promising brain injury biomarkers. The authors provide an update on the current knowledge of TBI biomarkers, especially protein biomarkers for neuronal cell body injury (UCH-L1) and astroglial injury (GFAP, S100B), and a focused literature review dealing with implementation of mTBI biomarkers in clinical practice.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 4","pages":"323-338"},"PeriodicalIF":1.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correction: Chloroquine inhibits vasodilation induced by ATP-sensitive potassium channels in isolated rat aorta. 更正:氯喹抑制离体大鼠主动脉atp敏感钾通道诱导的血管舒张。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-07-01 DOI: 10.4149/gpb_2023014

Kyeong-Eon Park, Soo Hee Lee, Sung Il Bae, Yeran Hwang, Seong-Ho Ok, Dawon Kang, Seung Hyun Ahn, Gyujin Sim, Jin Kyeong Park, Ju-Tae Sohn

引用次数: 0

Targeting AMPK/eNOS pathway and mitochondria by sonlicromanol protects myocardial cells against ischemia-reperfusion injury. sonlicromanol靶向AMPK/eNOS通路和线粒体对心肌细胞缺血再灌注损伤具有保护作用。

IF 1.5 4区生物学

General physiology and biophysics Pub Date : 2023-07-01 DOI: 10.4149/gpb_2023003

Kun Liao, Fangfei Huang, Xing Xie, Linhui Li

{"title":"Targeting AMPK/eNOS pathway and mitochondria by sonlicromanol protects myocardial cells against ischemia-reperfusion injury.","authors":"Kun Liao, Fangfei Huang, Xing Xie, Linhui Li","doi":"10.4149/gpb_2023003","DOIUrl":"https://doi.org/10.4149/gpb_2023003","url":null,"abstract":"This work evaluated the cardioprotective effects of sonlicromanol, a new mitochondrial-directed drug, on cardiac ischemia/reperfusion (I/R) injury and explored the involvement of inflammatory and oxidative responses via activation of AMPK-eNOS-mitochondrial pathway. Male Sprague-Dawley rats underwent regional I/R injury through in vivo left anterior descending (LAD) coronary artery ligation for 40 minutes followed by 24 hours of reperfusion. Pretreatment of rats with sonlicromanol considerably reduced cardiac I/R injury in a dose-dependent manner, as indicated by lower infarct size and serum creatine-kinase levels, and improved cardiac function after reperfusion. Sonlicromanol (50 mg/kg) significantly reduced TNF-α, interleukin-1β, NF-κB-p65, and 8-isoprostane levels while increased manganese-superoxide dismutase and nitric-oxide levels and expression of eNOS and AMPK protein. It significantly reduced mitochondrial membrane depolarization and reactive oxygen species (ROS) levels. However, AMPK inhibition significantly reduced sonlicromanol protective actions. Cardioprotection by sonlicromanol was achieved by moderating inflammatory and oxidative responses, and AMPK/eNOS/mitochondrial signaling is a crucial regulator of these actions.","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"42 4","pages":"373-382"},"PeriodicalIF":1.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9813956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0