内质网应激相关基因在非酒精性脂肪肝的诊断和亚型分类中的作用。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Zihao Guo, Xiaoxiao Yu, Zhihao Fang, Kai Yang, Changxu Liu, Zhichao Dong, Chang Liu
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引用次数: 0

摘要

非酒精性脂肪肝(NAFLD)是全球发病率最高的肝病。肝细胞中内质网应激(ERS)的慢性激活可能会促进非酒精性脂肪肝的发生,但尚未对非酒精性脂肪肝中的内质网应激相关基因(ERSGs)进行研究。我们的目的是研究ERSGs与非酒精性脂肪肝患者免疫微环境之间的关系,并构建预测模型。我们利用两个 GEO 数据集和 GeneCards 数据库的数据筛选了 48 个内质网应激相关差异表达基因(ERSR-DEGs)。富集分析表明,ERSR-DEGs与免疫相关通路和功能密切相关。通过单样本基因组富集分析(ssGSEA)获得了非酒精性脂肪肝的免疫浸润特征。非酒精性脂肪肝组与对照组在免疫细胞浸润和免疫功能方面存在明显差异。利用 113 个非酒精性脂肪肝样本,我们探索了基于 ERSR-DEGs 的两个分子集群。五基因 SVM 模型被选为最佳机器学习模型,基于五基因 SVM 模型的提名图显示了良好的预测效率。POR、PPP1R15A、FOS和FAS的mRNA表达水平在非酒精性脂肪肝小鼠和健康小鼠之间存在显著差异。总之,ERS与非酒精性脂肪肝的发生密切相关。我们建立了一个基于 SVM 的预测模型,用于评估 ERS 亚型和非酒精性脂肪肝患者的患病风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of endoplasmic reticulum stress-related genes in the diagnosis and subtyping of non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent liver disease worldwide. Chronic activation of endoplasmic reticulum stress (ERS) in hepatocytes may promote the development of NAFLD, yet endoplasmic reticulum stress-related genes (ERSGs) have not been studied in NAFLD. Our aim is to study the relationship between ERSGs and the immune microenvironment of NAFLD patients and to construct predictive models. We screened 48 endoplasmic reticulum stress-related differentially expressed genes (ERSR-DEGs) using data from two GEO datasets and the GeneCards database. Enrichment analysis revealed that ERSR-DEGs are closely associated with immune-related pathways and functions. The immune infiltration profile of NAFLD was obtained by single sample gene set enrichment analysis (ssGSEA). There were significant differences in immune cell infiltration and immune function between NAFLD group and control group. Using 113 NAFLD samples, we explored two molecular clusters based on ERSR-DEGs. A five-gene SVM model was selected as the best machine learning model, and a nomogram based on five-gene SVM model showed good predictive efficiency. The mRNA expression levels of POR, PPP1R15A, FOS and FAS were significantly different between NAFLD mice and healthy mice. In conclusion, ERS is closely associated with the development of NAFLD. We established a promising and SVM-based predictive model to assess the risk of disease in patients with ERS subtypes and NAFLD.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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