Functional Plant Biology最新文献_第8页

Overexpression of the alfalfa (Medicago sativa) gene, MsKMS1, negatively regulates seed germination in transgenic tobacco (Nicotiana tabacum). 紫花苜蓿（Medicago sativa）基因 MsKMS1 的过表达对转基因烟草（Nicotiana tabacum）的种子萌发具有负调控作用。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP23210

Yiqi Wan, Yuman Cao, Zhiqiang Zhang, Bo Han, Maojin Lu, Zijie Zhuo, Xinyi Gao, Peizhi Yang, Yafang Wang

{"title":"Overexpression of the alfalfa (Medicago sativa) gene, MsKMS1, negatively regulates seed germination in transgenic tobacco (Nicotiana tabacum).","authors":"Yiqi Wan, Yuman Cao, Zhiqiang Zhang, Bo Han, Maojin Lu, Zijie Zhuo, Xinyi Gao, Peizhi Yang, Yafang Wang","doi":"10.1071/FP23210","DOIUrl":"10.1071/FP23210","url":null,"abstract":"Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-associated proteins are a class of transmembrane proteins involved in intracellular trafﬁcking pathways. However, the functions of many SNARE domain-containing proteins remain unclear. We have previously identified a SNARE-associated gene in alfalfa (Medicago sativa ) KILLING ME SLOWLY1 (MsKMS1 ), which is involved in various abiotic stresses. In this study, we investigated the function of MsKMS1 in the seed germination of transgenic tobacco (Nicotiana tabacum ). Phylogenetic analysis showed that MsKMS1 was homologous to the SNARE-associated or MAPR component-related proteins of other plants. Germination assays revealed that MsKMS1 negatively regulated seed germination under normal, D-mannitol and abscisic acid-induced stress conditions, yet MsKMS1 -overexpression could confer enhanced heat tolerance in transgenic tobacco. The suppressive effect on germination in MsKMS1 -overexpression lines was associated with higher abscisic acid and salicylic acid contents in seeds. This was accompanied by the upregulation of abscisic acid biosynthetic genes (ZEP and NCED ) and the downregulation of gibberellin biosynthetic genes (GA20ox2 and GA20ox3 ). Taken together, these results suggested that MsKMS1 negatively regulated seed germination by increasing abscisic acid and salicylic acid contents through the expression of genes related to abscisic acid and gibberellin biosynthesis. In addition, MsKMS1 could improve heat tolerance during the germination of transgenic tobacco seeds.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open field hardening improves leaf physiological drought tolerance in young plants of Sindora siamensis. 露地硬化提高了暹罗金丝桃幼苗叶片的生理耐旱性。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP23102

Warunya Paethaisong, Preeyanuch Lakhunthod, Supranee Santanoo, Natthamon Chandarak, Sujittra Onwan, Naruemol Kaewjampa, Anoma Dongsansuk

{"title":"Open field hardening improves leaf physiological drought tolerance in young plants of Sindora siamensis.","authors":"Warunya Paethaisong, Preeyanuch Lakhunthod, Supranee Santanoo, Natthamon Chandarak, Sujittra Onwan, Naruemol Kaewjampa, Anoma Dongsansuk","doi":"10.1071/FP23102","DOIUrl":"10.1071/FP23102","url":null,"abstract":"The effect of drought stress on leaf physiology was studied in 10-month-old plants of Sindora siamensis . Plants were either placed in an open greenhouse (unhardening; UH) or in an open field (open field hardening; H) for 45days. Both the UH and H plants stopped receiving water (D) until the initial drought injury and then rewatered (R) until complete recovery. Results showed necrosis in the leaves of UH+D, while H+D showed wilting at Day 7 after drought. A greater degree of necrosis was found in UH+D+R but made complete recovery in H+D+R at Day 4 after rewatering. Drought stress resulted in decreased leaf area in H, and reduced leaf and stem water status, PSII efficiency, net photosynthetic rate, stomatal conductance and transpiration rate in both UH and H. It also resulted in an increase in water use efficiency in both UH and H. Electrolyte leakage and malondialdehyde contents in UH were markedly increased due to drought stress. These results suggest that unhardened young plants of Sindora exposed to drought exhibited enhanced stomata behaviour by minimising open stomata and transpiration, resulting in high efficiency of water usage. However, there was still membrane damage from lipid peroxidation, which caused necrosis. Open field hardened plants exposed to drought demonstrated reduced open stomata and transpiration, thereby preserving leaf and soil water status and enhancing water use efficiency. This may be a reduction in lipid peroxidation though an oxidative scavenging mechanism that causes a slight alteration in membrane stability and a slight necrosis.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the effect of drought stress and methanol spraying on the influential genes in the Calvin cycle and photorespiration of rapeseed (Brassica napus). 研究干旱胁迫和甲醇喷洒对油菜卡尔文循环和光蒸腾影响基因的影响

IF 2.6 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP23280

Parisa Taghvimi, Mohammad Mohsenzadeh Golfazani, Mohammad Mahdi Taghvaei, Habibollah Samizadeh Lahiji

{"title":"Investigating the effect of drought stress and methanol spraying on the influential genes in the Calvin cycle and photorespiration of rapeseed (Brassica napus).","authors":"Parisa Taghvimi, Mohammad Mohsenzadeh Golfazani, Mohammad Mahdi Taghvaei, Habibollah Samizadeh Lahiji","doi":"10.1071/FP23280","DOIUrl":"10.1071/FP23280","url":null,"abstract":"Due to global warming and changes in precipitation patterns, many regions are prone to permanent drought. Rapeseed (Brassica napus ) is one of the main sources of edible oils worldwide, and its production and yield are affected by drought. In this study, gene expression alterations under drought stress are investigated with bioinformatics studies to examine evolutionary relations of conserved motifs structure and interactions among Calvin cycle and photorespiration pathways key genes in drought-tolerant (SLM046) and drought-sensitive (Hayola308) genotypes of rapeseed. Investigating the conservation and evolutionary relationships revealed high conservation in motifs of FBPase, PRK, GlyK and NADP-ME enzymes. The analysis of protein interactions showed the correlation between FTRC, FBPase1, PRKX1, GlyKX2 and NADP-ME4 genes. Furthermore, in rapeseed, for the GlyKX2 and NADP-ME4 genes, four microRNAs of the miR172 family and four members of the miR167 family were identified as post-transcriptional regulators, respectively. The expression of ferredoxin thioredoxin reductase, fructose-1,6-bisphosphatase genes, phosphoribulokinase, glycerate kinase and malic enzyme 4 genes in the two rapeseed genotypes were evaluated by real-time qPCR method under 72h of drought stress and methanol foliar application. As a result, the highest expression levels of FTRC, PRKX1, GlyKX2, NADP-ME4 and FBPase1 were observed in methanol foliar application on the SLM046 genotype at 24h. In contrast, in methanol foliar application on the Hayola308 genotype, the highest expression levels of FTRC, PRKX1, GlyKX2, NADP-ME4 and FBPase1 were observed 8h after the treatment. Our study illustrated that methanol foliar application enhanced plant tolerance under drought stress.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flooding stress and responses to hypoxia in plants. 洪水胁迫和植物对缺氧的反应

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP24061

Juan de la Cruz Jiménez, Angelika Mustroph, Ole Pedersen, Daan A Weits, Romy Schmidt-Schippers

{"title":"Flooding stress and responses to hypoxia in plants.","authors":"Juan de la Cruz Jiménez, Angelika Mustroph, Ole Pedersen, Daan A Weits, Romy Schmidt-Schippers","doi":"10.1071/FP24061","DOIUrl":"10.1071/FP24061","url":null,"abstract":"In recent years, research on flooding stress and hypoxic responses in plants has gathered increasing attention due to climate change and the important role of O2 in metabolism and signalling. This Collection of Functional Plant Biology on 'Flooding stress and responses to hypoxia in plants' presents key contributions aimed at progressing our current understanding on how plants respond to low-O2 conditions, flooding stress and a combination of stresses commonly found in flooded areas. The Collection emphasises the characterisation of diverse plant responses across different developmental stages, from seed germination to fully developed plants, and under different water stress conditions ranging from waterlogging to complete submergence, or simply low-O2 conditions resulting from limited O2 diffusivity in bulky tissues. Additionally, this Collection highlights diverse approaches, including eco-physiological characterisation of plant responses, detailed descriptions of root anatomical characteristics and their surrounding microenvironments, evaluation of the seed microbiota under flooding stress, the modification of gene expression, and evaluations of diverse germplasm collections.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root photosynthesis prevents hypoxia in the epiphytic orchid Phalaenopsis. 根部光合作用可防止附生兰蝴蝶兰缺氧。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP23227

Luca Brunello, Ester Polverini, Giulia Lauria, Marco Landi, Lucia Guidi, Elena Loreti, Pierdomenico Perata

{"title":"Root photosynthesis prevents hypoxia in the epiphytic orchid Phalaenopsis.","authors":"Luca Brunello, Ester Polverini, Giulia Lauria, Marco Landi, Lucia Guidi, Elena Loreti, Pierdomenico Perata","doi":"10.1071/FP23227","DOIUrl":"10.1071/FP23227","url":null,"abstract":"Orchids (Phalaenopsis spp.) growing in tropical and subtropical regions are epiphytes. As such, they grow on trees with the root system utilised to anchor themselves to tree branches. These roots are highly specialised, display a large diameter and are often green, suggesting the ability to carry out photosynthesis. However, the role of photosynthesis in orchid roots is controversial. Orchids that are leafless can photosynthesise in their roots, thus indicating that some orchid roots carry out photosynthesis in a similar manner to leaves. However, the primary site of photosynthesis in orchids are in their leaves, and the roots of epiphytic orchids may mostly conduct internal refixation of respiratory CO2 . Besides contributing to the overall carbon metabolism of orchid plants, oxygen produced through root photosynthesis may also be important by alleviating potential root hypoxia. The bulky tissue of most epiphytic orchid roots suggests that oxygen diffusion in these roots can be limited. Here, we demonstrate that the bulky roots of a widely commercially cultivated orchid belonging to the genus Phalaenopsis are hypoxic in the dark. These roots are photosynthetically active and produce oxygen when exposed to light, thus mitigating root hypoxia.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted metabolomic analysis of the metabolites in roots of Pugionium cornutum seedlings under drought stress. 干旱胁迫下玉米须秧苗根部代谢物的非靶向代谢组学分析

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP22190

Zhaoxin Wu, Ping Wang, Guihua Chen

{"title":"Untargeted metabolomic analysis of the metabolites in roots of Pugionium cornutum seedlings under drought stress.","authors":"Zhaoxin Wu, Ping Wang, Guihua Chen","doi":"10.1071/FP22190","DOIUrl":"10.1071/FP22190","url":null,"abstract":"Pugionium cornutum is an annual or biennial xerophyte distributed in arid regions, with drought resistance properties. While previous studies have predominantly focused on the physiological changes of P. cornutum , the understanding of its metabolite variations remains limited. In this study, untargeted metabolomic technology was performed to analyse the change of metabolites in the roots of P. cornutum seedlings under drought stress. Our findings revealed that compared to the R1, the root water potential and the number of lateral roots increased, while the length of the tap root and fresh weight increased first and then decreased. In the R1-R2, a total of 45 differential metabolites (DMs) were identified, whereas in the R1-R3 82 DMs were observed. Subsequently, KEGG analysis revealed a significant enrichment of microbial metabolism in diverse environments and aminobenzoate degradation in the R1-R2, and phenylpropanoid biosynthesis, ubiquinone, and other terpenoid-quinone biosynthesis and isoquinoline alkaloid biosynthesis were significantly enriched in the R1-R3. The upregulation DMs, including L-arginosuccinate, L-tyrosine, p-coumarate, caffeate, ferulate, vanillin, coniferin, 5-aminopentanoate, 2-methylmaleate and 2-furoate in P. cornutum seedlings may play a crucial role in enhancing root growth and improving drought resistance. These findings provide a basis for future investigations into the underlying mechanisms of drought resistance in P. cornutum .","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and biochemical assortment in different wheat genotypes (Triticum aestivum L.) under rain fed conditions. 雨水灌溉条件下不同小麦基因型（Triticum aestivum L.）的生理和生化特性。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-03-01 DOI: 10.1071/FP23252

Syeda Okasha Javed, Shahid Iqbal Awan, Sania Shouket, Kotb A Attia, Zhu Xi, Arif Ahmed Mohammed, Sher Aslam Khan, Sayeda Tanavish Javed, Yasir Majeed

{"title":"Physiological and biochemical assortment in different wheat genotypes (Triticum aestivum L.) under rain fed conditions.","authors":"Syeda Okasha Javed, Shahid Iqbal Awan, Sania Shouket, Kotb A Attia, Zhu Xi, Arif Ahmed Mohammed, Sher Aslam Khan, Sayeda Tanavish Javed, Yasir Majeed","doi":"10.1071/FP23252","DOIUrl":"10.1071/FP23252","url":null,"abstract":"Wheat (Triticum aestivum L.) is the most extensively cultivated cereal crop in the world; however, its growth and development are affected by different types of biotic and abiotic stress conditions. The aim of this study was to assess the physico-chemical diversity in different wheat genotypes under rain-fed conditions. Principle component analysis (PCA) showed that significant variation for different components contributed 77.87% of total variability among all genotypes. In the scree plot, the first two PCs (PC1=44.75%, PC2=14.28%) had significant differences for numerous agronomic traits. The scatter biplot depicted eight genotypes (Zardana, NR-462, D-97, BARS-2009 (a check), NR-481, Tarnab-73, NR-489 and Pirsabak-91) with high diversity (variation ~90%) for different morphological traits, identifiable as they were located further away from the origin than other genotypes. Factor analysis of loading factors among wheat genotypes across different morpho-physiological traits also showed significant diversity for positive and negative loads. In cluster analysis, genotypes such as BWP-97, BARS-2009, NR-489, NR-448 and Pak. 13 were outliers, indicating significant diversity among all genotypes for different agronomic traits. Biochemical analysis showed maximum values for antioxidant activity, total phenolic content, and total flavonoid content in lines NR-485 (93.76%), NR-489 (3.55mg gallic acid equivalent (GAE)/g), and the variety Suleman-96 (3.45mg quercetin equivalent (QE)/g), respectively. This study provides new insights for understanding the diversity of different wheat genotypes under rain-fed conditions, and the selected genotypes can be evaluated for further breeding programs.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introducing the halophyte Salicornia europaea to investigate combined impact of salt and tidal submergence conditions. 引入盐生植物 Salicornia europaea，研究盐和潮汐淹没条件的综合影响。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-02-01 DOI: 10.1071/FP23228

Angelina Jordine, Julia Retzlaff, Lina Gens, Brigitta Ehrt, Lisa Fürtauer, Joost T van Dongen

{"title":"Introducing the halophyte Salicornia europaea to investigate combined impact of salt and tidal submergence conditions.","authors":"Angelina Jordine, Julia Retzlaff, Lina Gens, Brigitta Ehrt, Lisa Fürtauer, Joost T van Dongen","doi":"10.1071/FP23228","DOIUrl":"10.1071/FP23228","url":null,"abstract":"Tolerance mechanisms to single abiotic stress events are being investigated in different plant species, but how plants deal with multiple stress factors occurring simultaneously is still poorly understood. Here, we introduce Salicornia europaea as a species with an extraordinary tolerance level to both flooding and high salt concentrations. Plants exposed to 0.5MNaCl (mimicking sea water concentrations) grew larger than plants not exposed to salt. Adding more salt reduced growth, but concentrations up to 2.5MNaCl were not lethal. Regular tidal flooding with salt water (0.5MNaCl) did not affect growth or chlorophyll fluorescence, whereas continuous flooding stopped growth while plants survived. Quantitative polymerase chain reaction (qPCR) analysis of plants exposed to 1% oxygen in air revealed induction of selected hypoxia responsive genes, but these genes were not induced during tidal flooding, suggesting that S. europaea did not experience hypoxic stress. Indeed, plants were able to transport oxygen into waterlogged soil. Interestingly, sequential exposure to salt and hypoxic air changed the expression of several but not all genes as compared to their expression upon hypoxia only, demonstrating the potential to use S . europaea to investigate signalling-crosstalk between tolerance reactions to multiple environmental perturbations.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the mechanistic basis of plant adaptation to salinity and drought. 了解植物适应盐度和干旱的机理基础。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-02-01 DOI: 10.1071/FP23216

Muhammad Waseem, Mehtab Muhammad Aslam, Sunil Kumar Sahu

{"title":"Understanding the mechanistic basis of plant adaptation to salinity and drought.","authors":"Muhammad Waseem, Mehtab Muhammad Aslam, Sunil Kumar Sahu","doi":"10.1071/FP23216","DOIUrl":"10.1071/FP23216","url":null,"abstract":"Plant growth and development is adversely affected by environmental constraints, particularly salinity and drought. Climate change has escalated the effect of salinity and drought on crops in varying ways, affecting agriculture and most importantly crop productivity. These stressors influence plants across a wide range of levels, including their morphology and physiological, biochemical, and molecular processes. Plant responses to salinity and drought stress have been the subject of intense research being explored globally. Considering the importance of the impact that these stresses can have on agriculture in the short term, novel strategies are being sought and adopted in breeding programs. Better understanding of the molecular, biochemical, and physiological responses of agriculturally important plants will ultimately help promote global food security. Moreover, considering the present challenges for agriculture, it is critical to consider how we can effectively transfer the knowledge generated with these approaches in the laboratory to the field, so as to mitigate these adversities. The present collection discusses how drought and salinity exert effects on plants.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and expression analysis of SBP-Box-like (SPL) gene family disclose their contribution to abiotic stress and flower budding in pigeon pea (Cajanus cajan). SBP-Box-like (SPL) 基因家族的鉴定和表达分析揭示了它们对鸽子豆（Cajanus cajan）非生物胁迫和花芽萌发的贡献。

IF 3 4区生物学

Functional Plant Biology Pub Date : 2024-02-01 DOI: 10.1071/FP23237

Tayyaba Shaheen, Abdul Rehman, Amany H A Abeed, Muhammad Waqas, Asad Aslam, Farrukh Azeem, Muhammad Qasim, Muhammad Afzal, Muhammad Farooq Azhar, Kotb A Attia, Asmaa M Abushady, Sezai Ercisli, Nazia Nahid

{"title":"Identification and expression analysis of SBP-Box-like (SPL) gene family disclose their contribution to abiotic stress and flower budding in pigeon pea (Cajanus cajan).","authors":"Tayyaba Shaheen, Abdul Rehman, Amany H A Abeed, Muhammad Waqas, Asad Aslam, Farrukh Azeem, Muhammad Qasim, Muhammad Afzal, Muhammad Farooq Azhar, Kotb A Attia, Asmaa M Abushady, Sezai Ercisli, Nazia Nahid","doi":"10.1071/FP23237","DOIUrl":"10.1071/FP23237","url":null,"abstract":"The SPL gene family (for Squamosa Promoter-binding like Proteins) represents specific transcription factors that have significant roles in abiotic stress tolerance, development and the growth processes of different plants, including initiation of the leaf, branching and development of shoot and fruits. The SPL gene family has been studied in different plant species; however, its role is not yet fully explored in pigeon pea (Cajanus cajan ). In the present study, 11 members of the CcSPL gene family were identified in C. cajan . The identified SPLs were classified into nine groups based on a phylogenetic analysis involving SPL protein sequences from C. cajan , Arabidopsis thaliana , Cicer arietinum , Glycine max , Phaseolus vulgaris , Vigna unguiculata and Arachis hypogaea . Further, the identification of gene structure, motif analysis, domain analysis and presence of cis -regulatory elements in the SPL family members were studied. Based on RNA-sequencing data, gene expression analysis was performed, revealing that CcSPL2.1, 3 and 13A were significantly upregulated for salt-tolerance and CcSPL14 and 15 were upregulated in a salt-susceptible cultivar. Real-time qPCR validation indicated that CcSPL3, 4, 6 and 13A were upregulated under salt stress conditions. Therefore, molecular docking was performed against the proteins of two highly expressed genes (CcSPL3 and CcSPL14 ) with three ligands: abscisic acid, gibberellic acid and indole-3-acetic acid. Afterward, their binding affinity was obtained and three-dimensional structures were predicted. In the future, our study may open avenues for harnessing CcSPL genes in pigeon pea for enhanced abiotic stress resistance and developmental traits.","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0