Functional Plant Biology最新文献

{"title":"Corrigendum to: Trichome mediated external water transport may compensate for reduced vascular efficiency in atmospheric epiphytic Bromeliaceae.","authors":"Narcy Anai Pereira-Zaldívar, Luis David Patiño-López, Raúl Rodríguez-García, José Luis Andrade, Manuel Jesús Cach-Pérez, Celene Espadas-Manrique, Felipe Barredo-Pool, Casandra Reyes-García","doi":"10.1071/FP25140_CO","DOIUrl":"10.1071/FP25140_CO","url":null,"abstract":"","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":"52 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145741875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From evolution to function: a CYC/TB1 transcription factor is a key negative regulator of tillering in Setaria viridis.","authors":"Lingxin Zhang, Jinjing Wang, Hanchi Gao, Aihua Wang, Guo Wei, Yanjun Guo, Wangdan Xiong","doi":"10.1071/FP25257","DOIUrl":"10.1071/FP25257","url":null,"abstract":"<p><p>The TCP transcription-factor family, unique to the plant kingdom, is instrumental in the regulation of plant growth and development. Within this family, the CYC/TB1 clade has been shown to be particularly important in the tillering process of grasses. However, the evolutionary trajectory and functional divergence of CYC/TB1 remain unexplored in Setaria. Here, eight high-quality grass genomes were surveyed and 223 TCP genes were retrieved, allocating 105, 78, and 40 members to the PCF, CIN, and CYC/TB1 subfamilies, respectively. Many TCP genes, including those of the CYC/TB1 class, have undergone purifying selection during evolution, indicating a high degree of functional conservation. Within S. viridis, three CYC/TB1 genes are identified, and they were expressed tissue specifically, with Sevir.9G122200 expressing highly in tiller buds. Heterologous overexpression of Sevir.9G122200 in rice markedly suppresses early axillary shoot formation, demonstrating its conserved repressor activity. This study establishes the first evolutionary framework for the CYC/TB1 clade in Setaria and identifies it as a negative regulator of tillering, providing targets for functional genomics and breeding in related cereals.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential ozone-induced alterations in the photosynthetic apparatus and chlorophyll fluorescence dynamics of two pea cultivars (Pisum sativum L.).","authors":"Gereraj Gupta, Parvati Madheshiya, Supriya Tiwari","doi":"10.1071/FP25247","DOIUrl":"10.1071/FP25247","url":null,"abstract":"<p><p>The phytotoxic nature of tropospheric ozone (O3) has led to substantial declines in global crop productivity. Given their nutritional richness, legumes serve as vital dietary staples, valued for their high protein, oil, and fiber content. A field experiment was conducted to assess the physiological plasticity of two pea (Pisum sativum L.) cultivars, K Agaiti (O3-sensitive) and K Damini (O3-tolerant), under elevated O3 (ambient + 15 ppb). The Ball-Woodrow-Berry (BWB) model revealed a greater change in the intercept for K Agaiti compared to K Damini, indicating stronger stomatal disruption. Analysis of net CO2 assimilation rate, A, versus calculated internal CO2 concentrations, Ci (A/Ci), and photosynthetic active radiation, Q (A/Q), curves and chlorophyll fluorescence transients demonstrated cultivar-specific variations in both light and dark reactions. K Agaiti exhibited significant photosystem II impairment, whereas K Damini showed moderate reductions linked to feedback inhibition of dark reactions. Vcmax of elevated O3-exposed plants decreased by 3.5% and 7.35% at the vegetative stage, and by 4.91% and 8.45% at the reproductive stage in K Damini and K Agaiti, respectively. Similarly, seed weight per plant declined by 2.55% in K Damini and 15.29% in K Agaiti. These findings highlight the resilience of K Damini's photosynthetic machinery under O3 stress, demonstrating cultivar-specific adaptability and providing insight into the physiological basis of ozone tolerance in pea.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of a novel bark-specific multi-product monoterpene synthase from the rubber tree (Hevea brasiliensis).","authors":"Huong Thi Diem Tran, Thai Nguyen Hong Le, Binh Thi My Pham, Thuong Thi Hong Nguyen","doi":"10.1071/FP25129","DOIUrl":"10.1071/FP25129","url":null,"abstract":"<p><p>The rubber tree (Hevea brasiliensis) is highly valued for its wood and latex. Along with being a rich source of polyterpenes in the latex, rubber trees are notable for their significant emission of volatile monoterpenes that may contribute to its chemical defense. A previous in silico genome-wide analysis of Hevea brasiliensis identified 47 putative terpene synthase (HbTPS) genes, of which only one had been experimentally characterized. In this study, we report the isolation and functional characterization of HbTPS24, a new monoterpene synthase belonging to the HbTPS family, providing new insights into the molecular basis of monoterpene biosynthesis in this species. Phylogenetic analysis placed HbTPS24 in the TPS-b subfamily. In vitro enzymatic assays revealed that HbTPS24 is multi-functional, converting both neryl diphosphate and geranyl diphosphate into cyclic monoterpenes such as α-thujene, β-pinene, α-terpinene, α-terpinolene, and α-terpineol. qRT-PCR analysis showed that HbTPS24 is mainly expressed in the bark, with significantly lower levels in the leaves and undetectable levels in the latex. The predominant expression of HbTPS24 in bark tissues, the detection of specific monoterpenes such as D-limonene and α-pinene in bark extracts, along with the broader range of cyclic monoterpenes identified in both in vitro enzymatic assays and canopy-scale volatile flux measurements, strongly suggest that HbTPS24 contributes to the in planta synthesis of multiple cyclic monoterpenes. These compounds are potentially involved in mediating stress resilience in Hevea brasiliensis.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between environmental origin, phylogenetic relatedness and root system architecture (RSA) in wild lentil species.","authors":"Salma Rouichi, Romain Fernandez, Alain Audebert, Margaux Legoux, Moez Amri, Michel Edmond Ghanem","doi":"10.1071/FP25180","DOIUrl":"10.1071/FP25180","url":null,"abstract":"<p><p>Root System Architecture (RSA) plays a central role in plant performance by regulating water and nutrient uptake. As agriculture faces increasing challenges from environmental variability, nutrient limitation and water scarcity, identifying adaptive root traits in wild relatives is critical for developing resilient crop varieties. We screened a diverse panel of cultivated and wild lentil (Lens spp.) accessions using the Rhizoscope, a high-throughput root phenotyping system developed by CIRAD. In total, 42 wild accessions and eight advanced breeding lines were evaluated for RSA traits and quantified at 30 days after sowing using a rhizobox-based phenotyping platform. Our objectives were to assess RSA variation within wild species and compare RSA traits between cultivated and wild genotypes. Cultivated lentil showed higher values for traits such as root mass, diameter, root volume, root angle (RA) and maximum root depth (MRD), suggesting greater resource acquisition efficiency. In contrast, wild accessions exhibited higher root:shoot ratios and Collar-First Ramification length (CRL), consistent with adaptation to resource-limited environments. To understand the drivers of RSA variation, we incorporated environmental variables from the center of origin of each accession, including Aridity Index, soil type and bedrock depth, into multivariate analyses using Linear Discriminant Analysis and Classification and Regression Trees. Results showed that variation in traits such as MRD, RA and CRL was more strongly linked to environmental conditions than species classification. Deeper roots were associated with arid regions and deep bedrock, while wider RAs and shorter CRL lengths were typical of genotypes from compacted or shallow soils. These findings suggest that RSA traits in wild lentil species are shaped primarily by local environmental selection rather than taxonomic identity. This highlights the importance of integrating ecological provenance with phenotypic assessments when evaluating wild germplasm. Relying solely on species classification may overlook key adaptive traits. Incorporating environmental data can improve the identification of genotypes with root traits conferring tolerance to drought and edaphic stress, thereby supporting the development of more resilient lentil cultivars.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":"52 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145563376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cassava mosaic disease impairs photosynthesis and sugar metabolism: varietal susceptibility and key metabolic disruptions.","authors":"Saravanan Raju, Makeshkumar Thangaraj, Aparna Rajan, Senthilkumar K Muthusamy, Resmi Nair, Velumani Ravi","doi":"10.1071/FP25184","DOIUrl":"10.1071/FP25184","url":null,"abstract":"<p><p>Cassava mosaic disease (CMD) is a major threat to cassava production, causing significant disruptions in photosynthesis, carbohydrate metabolism, and leaf structure, severely reducing crop productivity. This study investigates CMD's physiological, biochemical, and structural impacts across five cassava genotypes, revealing substantial differences in CMD susceptibility. Genotypes such as Sree Visakham and Sree Vijaya exhibited greater tolerance, maintaining higher chlorophyll content, enhanced non-photochemical quenching, and less severe structural alterations, suggesting tolerance mechanisms against CMD. In contrast, Sree Athulya showed rapid viral accumulation and steep declines in photosynthetic efficiency, stomatal function, and carbohydrate synthesis. The study highlights CMD's disruption of source-sink dynamics, with significant reductions in starch content and altered sugar accumulation in susceptible varieties, indicating impaired carbon allocation. Confocal and scanning electron microscopy analyses provided direct evidence of CMD-induced chloroplast damage and distortions in the epidermal structure, further compounding the physiological stress. These findings emphasize the need for breeding strategies incorporating CMD tolerance traits, such as enhanced photosynthetic efficiency and structural resilience, to develop resistant cassava varieties. Such efforts are critical for ensuring food security in regions dependent on cassava as a staple crop.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin-mediated functional responses to water deficit in Phaseolus vulgaris L.: mechanistic insights across physiological, biochemical, and genomic scales.","authors":"Gamal A Zayed, Abdel-Haleem A H El-Shaieny, Hoida Zaki","doi":"10.1071/FP25301","DOIUrl":"10.1071/FP25301","url":null,"abstract":"<p><p>Agricultural productivity is increasingly constrained by water scarcity, which affects nearly one-quarter of cultivated land and is projected to intensify due to climate change and escalating freshwater demands. Melatonin is widely recognized as a potent biostimulant that plays a crucial role in mitigating various abiotic stresses, particularly drought, across many plant species. This study demonstrates that exogenous application of melatonin (150 μM; foliar spray) confers protection to Phaseolus vulgaris under moderate water deficit (40% field capacity). A randomized complete block design comprising four treatment groups (n = 30 seedlings per group, five replicates) was employed to systematically evaluate morphological, physiological, biochemical, and molecular responses. Melatonin applications at 21 and 28 days after sowing significantly enhanced shoot elongation, leaf area expansion, and photosynthetic efficiency. Biochemically, melatonin markedly increased the activities of key antioxidant enzymes (ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD)), reduced reactive oxygen species accumulation, elevated proline content by 24%, and decreased electrolyte leakage by 18%, thereby improving osmotic balance and maintaining membrane integrity. Genomic stability was assessed using Inter-Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers, revealing that melatonin substantially attenuated drought-induced DNA damage. Marker analysis further demonstrated differential sensitivity, and key statistical indices, including polymorphism information content (PIC), effective multiplex ratio (EMR), and resolving power (RP), exhibited strong linear associations, reinforcing the reliability of molecular diagnostics. Collectively, these results highlight melatonin's multifaceted role in enhancing water-deficit resilience through integrated regulation of physiological homeostasis, oxidative stress mitigation, and genome protection. The findings support melatonin's practical potential as a low-cost, environmentally compatible strategy for improving legume performance in water-deficit environments.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145667705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of SDH, QDH, and PAL in species, accumulating different types of tannins.","authors":"Anna Sereda, Sergey Bondarev, Grigory Adamov, Dmitry Baleev, Eugenia Nikonorova","doi":"10.1071/FP25194","DOIUrl":"10.1071/FP25194","url":null,"abstract":"<p><p>The biosynthesis of different classes of tannins in plants is a complex process that remains insufficiently studied. This study aimed to evaluate the activity of shikimate dehydrogenase (SDH), quinate dehydrogenase (QDH), and phenylalanine ammonia-lyase (PAL), and their influence on the accumulation of proanthocyanidins (PA) and/or hydrolyzable tannins (HT) in medicinal plants. The leaves of Hippophae rhamnoides and Cornus sericea (accumulating HT), Astilbe chinensis (accumulating PA), and Agrimonia pilosa and Agrimonia asiatica (accumulating both HT and PA) were used in the present study. The highest PA levels were found in A. chinensis, whereas the highest HT content was observed in H. rhamnoides. Water-soluble PA predominated in all species. The highest SDH activities were detected in the leaves of the HT-rich species C. sericea and H. rhamnoides, whereas QDH activity was comparatively low. The activity of PAL was the highest in the leaves of H. rhamnoides, and the lowest in A. chinensis. Correlation analysis revealed positive relationships between SDH and PAL activity and HT levels, and a negative relationship with PA levels. No significant correlation was found between QDH activity and tannin content. SDH activity positively correlated with the content of gallic acid and β-glucogallin, and negatively with catechin. No correlation was observed between PAL activity and the levels of catechin or epicatechin. Hierarchical clustering separated variables into two major groups: catechin, epicatechin, different fractions of PA, and QDH in one cluster, and SDH, PAL, HT, gallic acid, β-glucogallin, ellagic acid, pentagalloyl glucose, and chlorogenic acid in the other. Clustering by species revealed an HT-dominant group (H. rhamnoides and C. sericea) and a PA-dominant group (A. chinensis and Agrimonia spp.). These findings add valuable information to the enzymatic regulation of tannin biosynthesis in medicinal plants that accumulate different types of tannins.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic role of zinc oxide nanoparticles in enhancing antioxidative defense and drought tolerance via metabolic and essential oil modulation in marjoram.","authors":"Samar F Elsharkawy, Samia A Haroun, Mohamed A Abbas, Mahmoud R Sofy, Mona S Agha","doi":"10.1071/FP25177","DOIUrl":"10.1071/FP25177","url":null,"abstract":"<p><p>Drought is one significant environmental stress that has a detrimental impact on plant growth and lowers production. This study examined the role of ZnO₂ nanoparticles (NPs) in reducing the harmful effects of drought. The growth features of marjoram plants cultivated in typical and drought-stressed environments (100, 75, and 50% water field capacity) were increased by spraying with solutions containing nanoparticles of ZnO2. In contrast to other treated plants, plants treated with ZnO2 NPs (100 mg L-1) showed greater levels of important osmolytes and conserved more photosynthetic pigments. Furthermore, by raising the membrane stability index, the antioxidant enzymes' activity, and the levels of lipid peroxidation, hydrogen peroxide, and exogenous application of ZnO2 NPs spraying improved drought tolerance and reduced the membrane injury index. An increase in growth and biochemical parameters, such as total antioxidant activity and anthocyanin content, was detected in ZnO2 NPs-treated drought-stressed marjoram plants. Marjoram plant oil analysis revealed that some components appeared, such as eucalyptol, and others increased, especially in response to treatment with only the lowest water stress (50% WFC) and 100 mg L-1 ZnO2 NPs treatment. Our findings generally corroborated the beneficial effects of applying zinc NPs, particularly the absolute contribution of ZnO2 NPs (100 mg L-1) to lessen drought stress's negative consequences (specifically, 50% WFC) in marjoram plants.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":"52 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145430876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA regulatory networks in plant responses to combined heat and drought.","authors":"Bekzhan D Kossalbayev, Altynay Y Shayakhmetova, Assemgul K Sadvakasova, Serik Tursbekov, Dilnaz Zaletova, Gulzhanay K Kamshybayeva","doi":"10.1071/FP25272","DOIUrl":"10.1071/FP25272","url":null,"abstract":"<p><p>Global climate change poses a serious threat to agriculture, with heat and drought stress often occurring simultaneously and severely impacting crop productivity. As post-transcriptional regulators, microRNAs (miRNAs) mediate plant responses to these adverse conditions by targeting genes involved in antioxidant defense, growth, development, and hormonal signaling. However, research on miRNA roles under combined drought and heat stress is still limited compared to individual stress studies. Additionally, stress-, cultivar-, and tissue-specific expression patterns of miRNAs, along with discrepancies between controlled laboratory conditions and natural environments, complicate the development of broadly applicable miRNA-based strategies. This review explores recent advancements in understanding miRNA target genes and their functions, highlighting the need for innovative, sustainable solutions for crop improvement.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":"52 11","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}