Journal of Basic and Applied Genetics最新文献_第2页

VARIABILITY OF PDYN AND OPRK1 GENES IN FOUR ARGENTINIAN POPULATIONS AND ITS GENETIC ASSOCIATION WITH CLINICAL VARIABLES RELATED TO ACUTE POSTSURGICAL PAIN 四个阿根廷人群中pdyn和oprk1基因的变异及其与急性术后疼痛相关临床变量的遗传关联

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.01

G. P. Di Santo Meztler, J. Schiaffi, A. Rigalli, M. E. Esteban Torné, P. Martina, C. I. Catanesi

{"title":"VARIABILITY OF PDYN AND OPRK1 GENES IN FOUR ARGENTINIAN POPULATIONS AND ITS GENETIC ASSOCIATION WITH CLINICAL VARIABLES RELATED TO ACUTE POSTSURGICAL PAIN","authors":"G. P. Di Santo Meztler, J. Schiaffi, A. Rigalli, M. E. Esteban Torné, P. Martina, C. I. Catanesi","doi":"10.35407/bag.2022.33.02.01","DOIUrl":"https://doi.org/10.35407/bag.2022.33.02.01","url":null,"abstract":"Several population studies showed an association between variation in pain sensitivity and genetic polymorphisms located in Prodynorphin (PDYN) and Kappa Opioid Receptor (OPRK1) human genes. We analysed polymorphisms of these two genes to characterise their variation in Argentinian populations, as well as to evaluate their association with acute pain sensitivity. We studied 11 genetic markers in individuals from four locations in Argentina (Ciudad Autónoma de Buenos Aires, La Plata, Resistencia, and Misión Nueva Pompeya), calculated the population parameters, and evaluated the possible association among pain sensitivity, clinical, and genetic variables through a Generalised Estimating Equation model. High linkage disequilibrium was observed in the four populations for both genes, and significant differences were found among frequencies of Argentinian populations and those from other continents reported in the 1000 Genomes Project. Four PDYN gene polymorphisms from 3´ untranslated region and exon 4 showed association with acute pain sensitivity. One genotype of each of these polymorphisms was associated with a higher pain sensitivity, probably related with the activation of the N-methyl-D-aspartate (NMDA) receptors. We found a strong association with acute pain for the following clinical variables: 1) time after surgery, 2) intravenous klosidol supplied every 8 h, and 3) type of incision. Our results highlight the importance of a regional study of genetic variants which influence pain sensitivity and analgesic response.\u0000Key words: human populations, pain sensitivity, acute pain, genetic polymorphisms, genetic structure","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132696339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOROS

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.suppl.03

引用次数: 0

CYTOGENETICS OF CHILEAN LAND PLANTS (EMBRYOPHYTA): STATE-OF-THE-ART AND PROSPECTS 智利陆地植物(胚胎植物)的细胞遗传学:现状与展望

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.01

P. Jara Seguel, J. Urrutia Estrada

{"title":"CYTOGENETICS OF CHILEAN LAND PLANTS (EMBRYOPHYTA): STATE-OF-THE-ART AND PROSPECTS","authors":"P. Jara Seguel, J. Urrutia Estrada","doi":"10.35407/bag.2022.33.01.01","DOIUrl":"https://doi.org/10.35407/bag.2022.33.01.01","url":null,"abstract":"Chile is located in the south-western region of South America along the Pacific Ocean and contributes to the worldwide flora with ca. 6,120 species of Bryophyta, Pteridophyta, Pinophyta, Gnetophyta, and Magnoliophyta (1.9% of worldwide total species), exhibiting high endemism across all plant divisions. Little is known about the genetic diversity of Chilean land plants worldwide, including their cytogenetic and molecular characteristics. In 2012 we published the first state-of-the-art review in Cytogenetics of Chilean Angiosperms. The article gathered 78 publications from 1924 to 2010 accounting for approximately 139 species (2.8% of total Chilean species). The aim of this paper was to review the advances in cytogenetic studies of Chilean land plants, reporting additional cytogenetic data for species of four botanical divisions until 2020. Cytogenetic data were searched in the CPCD (Chilean Plants Cytogenetic Database). In total, we found 180 publications from both Chilean and foreign researchers. To date, cytogenetic data have been reported for 499 Chilean land plant species (8.2% of total) belonging to 244 genera and 117 families. In this context, the 2001-2020 period has been among the most productive regarding publications, with 74 available reports that include 163 additional species. Based on chromosome numbers, angiosperms and bryophytes registered the greatest diversity with 55 and 29 different 2n, respectively; both divisions having the greatest number of studied species. Given the importance of increasing information on Chilean land plants, it is expected that more publications will contribute to the knowledge of their cytogenetic diversity in the near future.\u0000\u0000Key words: chromosome banding, chromosome number, DNA C-value, karyotype morphology, polyploidy.","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123279798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHROMOSOMES OF TWO OLYRA L. SPECIES FROM MISIONES, ARGENTINA (POACEAE, BAMBUSOIDEAE, OLYREAE) 阿根廷misiones两种油桐的染色体研究(豆科，竹科，油桐科)

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.07

A. V. Reutemann, F. Eckers, J. Daviña, A. Honfi

引用次数: 0

EVOLUTIONARY DYNAMICS OF AUTOPOLYPLOIDS IN NATURAL POPULATIONS: THE CASE OF TURNERA SIDOIDES COMPLEX 自然种群中自多倍体的进化动力学:以田葵复合体为例

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.06

I. E. Kovalsky, G. Elías, S. A. Fernandez, E. Moreno, G.C. Silva, J. M. Roggero Luque, N.E.A. Almirón, C. Solís, A. Dabrio, G. Via DO Pico, J. G. Seijo, V. S. Solís Neffa

{"title":"EVOLUTIONARY DYNAMICS OF AUTOPOLYPLOIDS IN NATURAL POPULATIONS: THE CASE OF TURNERA SIDOIDES COMPLEX","authors":"I. E. Kovalsky, G. Elías, S. A. Fernandez, E. Moreno, G.C. Silva, J. M. Roggero Luque, N.E.A. Almirón, C. Solís, A. Dabrio, G. Via DO Pico, J. G. Seijo, V. S. Solís Neffa","doi":"10.35407/bag.2022.33.01.06","DOIUrl":"https://doi.org/10.35407/bag.2022.33.01.06","url":null,"abstract":"Turnera sidoides (x=7) is one of the few well-studied South American autopolyploid complexes. Since polyploidy has played a prominent role within this complex, ongoing studies in T. sidoides focus on understanding the mechanisms involved in the origin and the establishment of polyploids using integrative approaches. This paper synthesises the results of more than 20 years of research on this topic. Cytogenetics analysis provided evidences for the production of unreduced male and female gametes, supporting the hypothesis of bilateral sexual polyploidization as the mechanism of origin of polyploids in T. sidoides. The finding of viable triploids suggested that unilateral sexual polyploidization could also be an important mechanism for the origin of tetraploids in T. sidoides. The occurrence of plants continuously forming many unreduced gametes would play a key role in the establishment of neopolyploids in natural populations. Also, the higher number of propagules that tetraploids contribute to subsequent generations, the ability to multiply asexually by rhizomes, and the occurrence of occasional cases of self-compatibility and successful illegitimate crosses in polyploids increase the likelihood that a low frequency of neopolyploids can be maintained in natural populations of T. sidoides. In addition, integration of cytogeographic and genetic divergence data together with past niche modelling provided further insights supporting the hypothesis that historical climatic and geomorphological events provided favourable conditions for the establishment of autopolyploids, with the wider distribution of tetraploids of T. sidoides being the result of their range expansion. \u0000\u0000Key words: cytogeography, establishment, origin, polyploidy, unreduced gametes","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129019470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHROMOSOMIC STUDIES IN ZEPHYRANTHES CITRINA BAKER (AMARYLLIDACEAE), A POLYPLOID ORNAMENTAL 多倍体观赏植物西风花的染色体研究

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.08

J. Daviña, A. C. Gianini Aquino, O.A. Rodríguez Mata, E. Tapia-Campos, R. Barba-González, A. Honfi

引用次数: 0

DNA CONTENT AND CYTOGENETIC CHARACTERISTICS OF Gymnocalycium quehlianum (CACTACEAE) ALONG AN ALTITUDINAL GRADIENT 沿海拔梯度的毛茛(仙人掌科)DNA含量及细胞遗传学特征

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.04

P. Martino, E. D. Gurvich, M. L. Las Peñas

{"title":"DNA CONTENT AND CYTOGENETIC CHARACTERISTICS OF Gymnocalycium quehlianum (CACTACEAE) ALONG AN ALTITUDINAL GRADIENT","authors":"P. Martino, E. D. Gurvich, M. L. Las Peñas","doi":"10.35407/bag.2022.33.01.04","DOIUrl":"https://doi.org/10.35407/bag.2022.33.01.04","url":null,"abstract":"Important changes in vegetation types occur along elevational gradients. The genus Gymnocalycium is endemic to southern South America, and its species are distributed along elevational gradients. In particular, Gymnocalycium quehlianum is a globular cactus endemic to the Sierras de Córdoba. Studying cytogenetic aspects and DNA content in populations throughout their distribution is key to understanding the species. DNA content and cytogenetic characteristics were analyzed in four populations of G. quehlianum (615, 744, 948 and 1257 masl). The genome size in the four populations varied between 3.55 and 4.30 pg. The populations were diploid (2n = 22). All populations showed the karyotype formula of 10 metacentrics (m) + 1 submetacentric (sm). The species presented symmetrical karyotypes and constitutive heterochromatin CMA+/DAPI- associated with nucleolar organizing regions, always found in the first pair of m chromosomes. The 18-5.8-26S rDNA locus is found in the terminal regions of the first pair of chromosomes m, and the 5S locus is adjacent to the 18-5.8-26S locus. A tendency for DNA content to decrease with increasing altitude was observed.\u0000\u0000Key words: Gymnocalycium quehlianum, chromosome number, cytogenetic, DNA content, altitudinal gradient","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115541950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

WHAT DO NEOTROPICAL PRIMATES TELL US UNDER THE LOOK OF CYTOGENETICS? 在细胞遗传学的视角下，新热带灵长类动物告诉我们什么?

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.09

E. R. Steinberg, M. J. Bressa, M. Mudry

{"title":"WHAT DO NEOTROPICAL PRIMATES TELL US UNDER THE LOOK OF CYTOGENETICS?","authors":"E. R. Steinberg, M. J. Bressa, M. Mudry","doi":"10.35407/bag.2022.33.01.09","DOIUrl":"https://doi.org/10.35407/bag.2022.33.01.09","url":null,"abstract":"Cytogenetics studies in Neotropical Primates (Primates: Platyrrhini) have shown that these mammals comprise a heterogeneous group at the chromosomal level. The remarkable variety of karyotypes described provides significant evidence on the possible role of chromosomal rearrangements in their evolution. In the Grupo de Investigación en Biología Evolutiva (GIBE), the line of research on the evolutionary divergence process in Platyrrhini considering different aspects of the organization of the genome has been established and developed uninterruptedly for more than 30 years. Among the advances made in recent years is the quantification of the genome size in six species of caí monkeys (Cebus sp.) and two species of howler monkeys (Alouatta sp.) and the description of the composition of base pairs in the constitutive heterochromatin regions in the genera Cebus and Ateles. The first descriptions were made of the karyotype and meiotic behavior in early prophase I of two species of howler monkeys, Alouatta caraya and A. guariba clamitans. In this last species, the first pentavalent-type sexual system X1X2X3Y1Y2 was identified in a primate species. The organization of euchromatin was characterized in terms of the content and distribution of AT and GC nucleotide bases in three species of howlers and in two species of caí monkeys. These, among other investigations, allowed contributing in an original way to the knowledge about speciation at different levels, as well as about the architecture and dynamics of the genome of these primates.\u0000\u0000Key words: Neotropical Primates, cytogenetics and taxonomy, chromosome evolution, sex chromosomes","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121891497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYTOGENETIC STUDY IN SAND SPIDERS (SICARIIDAE) FROM THE BRAZILIAN CAATINGA: SEX CHROMOSOME SYSTEM DIVERSITY IN CLOSELY RELATED SPECIES 巴西沙蛛的细胞遗传学研究:近缘种的性染色体系统多样性

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.05

T. Gimenez-Pinheiro, L. S. Carvalho, A. Brescovit, I. Magalhães, M. C. Schneider

{"title":"CYTOGENETIC STUDY IN SAND SPIDERS (SICARIIDAE) FROM THE BRAZILIAN CAATINGA: SEX CHROMOSOME SYSTEM DIVERSITY IN CLOSELY RELATED SPECIES","authors":"T. Gimenez-Pinheiro, L. S. Carvalho, A. Brescovit, I. Magalhães, M. C. Schneider","doi":"10.35407/bag.2022.33.01.05","DOIUrl":"https://doi.org/10.35407/bag.2022.33.01.05","url":null,"abstract":"In this study, we investigated the chromosomes of three species of Sicarius spiders from the Brazilian Caatinga, using classical and molecular cytogenetic techniques. Based on the phylogenetic approach, we also discussed about the variation of diploid number, types of sex chromosome system and changes in the localization of ribosomal genes of Scytodoidea. Sicarius are Synspermiata spiders that together with the genera Loxosceles and Hexophthalma constitute the family Sicariidae. In this group, the available cytogenetic data showed a low diploid number range (2n♂=18 to 2n♂=23) and the presence of only multiple sex chromosome systems (X1X2Y and X1X20). Mitotic metaphase cells exhibited 2n♂=16+X1X2Y for Sicarius cariri and S. ornatus, and 2n♂=18+XY for S. tropicus. In these species, silver impregnation revealed nucleolar organizer region (Ag-NOR) on the terminal region of pair 1. In S. ornatus and S. tropicus, the results obtained with fluorescent in situ hybridization (FISH) using 18S rDNA probe were similar to Ag-NOR, however in S. cariri, the ribosomal sites were localized in the terminal region of the X1 sex chromosome. In this work, we presented the first description of a simple sex chromosome system for Sicariidae, helping to understand how the XY sex chromosome system evolved from the X1X2Y system. Additionally, FISH data incongruous with Ag-NOR indicate that the cytogenetic studies in Sicariidae allow investigating the relation between the karyotype evolution and the distribution and the activity of rDNA genes. \u0000\u0000Key words: karyotype, mitosis, nucleolar organizer region, rDNA, Sicarius","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123067671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A BIRD´S-EYE VIEW OF CHROMOSOMES DURING MEIOTIC PROPHASE I 减数分裂前期I的染色体鸟瞰图

Journal of Basic and Applied Genetics Pub Date : 2022-07-01 DOI: 10.35407/bag.2022.33.01.02

María Inés Pigozzi

引用次数: 0