Journal of Basic and Applied Genetics最新文献_第7页

TEST OF INTERACTION IN THE ANALYSIS OF MOLECULAR VARIANCE 分子方差分析中的相互作用检验

Journal of Basic and Applied Genetics Pub Date : 2019-07-01 DOI: 10.35407/bag.2019.xxx.01.03

C. Bruno, M. Videla, M. Balzarini

{"title":"TEST OF INTERACTION IN THE ANALYSIS OF MOLECULAR VARIANCE","authors":"C. Bruno, M. Videla, M. Balzarini","doi":"10.35407/bag.2019.xxx.01.03","DOIUrl":"https://doi.org/10.35407/bag.2019.xxx.01.03","url":null,"abstract":"The genomic diversity, expressed in the differences between molecular haplotypes of a group of individuals, can be divided into components of variability between and within some factor of classification of the individuals. For such variance partitioning, molecular analysis of variance (AMOVA) is used, which is constructed from the multivariate distances between pairs of haplotypes. The classical AMOVA allows the evaluation of the statistical significance of two or more hierarchical factors and consequently there is no interaction test between factors. However, there are situations where the factors that classify individuals are crossed rather than nested, that is, all the levels of a factor are represented in each level of the other one. This paper proposes a statistical test to evaluate the interaction between crossed factors in a Non-Hierarchical AMOVA. The null hypothesis of interaction establishes that the molecular differences between individuals of different levels of a factor are the same for all the levels of the other factor that classifies them. The proposed analysis of interaction in a Non-Hierarchical AMOVA includes: calculation of the distance matrix and partition of it into blocks, subsequent calculation of residuals and analysis of non-parametric variance on the residuals. Its implementation is illustrated in simulated and real scenarios. The results suggest that the proposed interaction test for the Non-Hierarchical AMOVA presents high power. Key words: genetic variability, non-parametric methods, distances matrix, AMOVA.","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123097708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PARTIAL SEQUENCES OF THE GENE THAT CODIFIES FOR THE TRANSCRIPTION FACTOR VPHSFB1 IN Vasconcellea pubescens. FIRST REPORT 短毛输精管丛转录因子VPHSFB1编码基因的部分序列。第一份报告

Journal of Basic and Applied Genetics Pub Date : 2019-07-01 DOI: 10.35407/bag.2019.xxx.01.01

E. D. Arizala-Quinto, G. Viteri, F. M. Idrovo-Espín

引用次数: 1

CARYOLOGICAL STUDY OF Chrysobalanus icaco (CHRYSOBALANACEA) IN PARAÍSO, TABASCO, MÉXICO PARAÍSO, TABASCO, MÉXICO中黄颡鱼(chrysobalananus icaco)的石核学研究

Journal of Basic and Applied Genetics Pub Date : 2018-12-01 DOI: 10.35407/bag.2018.29.02.06

E.S. Méndez-Badal, R.F. Molina-Martínez, R. Hernández-Martínez, J. Lesher-Gordillo

{"title":"CARYOLOGICAL STUDY OF Chrysobalanus icaco (CHRYSOBALANACEA) IN PARAÍSO, TABASCO, MÉXICO","authors":"E.S. Méndez-Badal, R.F. Molina-Martínez, R. Hernández-Martínez, J. Lesher-Gordillo","doi":"10.35407/bag.2018.29.02.06","DOIUrl":"https://doi.org/10.35407/bag.2018.29.02.06","url":null,"abstract":"Chrysobalanus icaco is a plant that belongs to the Chrysobalanaceae family. In Tabasco, Mexico, this plant is distributed in the municipalities of the coastline. The main purpose of this research was to characterize the chromosomal structure of phenotypes with pink and purple fruits by the use of conventional staining. To this end, mature fruits were collected in the community of Pénjamo, Paraíso, Tabasco. Seeds were allowed to germinate for 30 days at a constant temperature of 30° C. Roots of 1.00 cm in length were subjected to acid hydrolysis and subsequent staining with orcein. A total of 295 metaphases were photographed and chromosomes of 35 metaphases of the purple phenotype and 30 of the pink phenotype were measured. Both phenotypes presented 18 small chromosomes, which were ordered in nine homologous pairs according to their relative size. No morphological or numerical differences were observed between the karyotypes of both phenotypes. In addition, the t-Student (α=0.05) test did not reveal significant differences between the chromosomes complements of both phenotypes.\u0000\u0000Key words:karyotype, tropical shrubs","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121305859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Isostichopus sp. IN THE COLOMBIAN CARIBBEAN SEA 哥伦比亚加勒比海异针藻的形态和分子特征

Journal of Basic and Applied Genetics Pub Date : 2018-12-01 DOI: 10.35407/bag.2018.29.02.04

W. Vergara, V. Agudelo, L. R. Castro, A. Rodríguez, I. Eeckhaut

引用次数: 3

NEW MITOCHONDRIAL MARKERS IMPROVE THE PHYLOGENY OF THE HAWKSBILL TURTLE Eretmochelys imbricata (TESTUDINES: CHELONIIDAE) 新的线粒体标记改善了斑鳖的系统发育(龟纲:龟科)

Journal of Basic and Applied Genetics Pub Date : 2018-12-01 DOI: 10.35407/bag.2018.29.02.03

G. C. Beltrán-Torres, J. Hernández-Fernández

{"title":"NEW MITOCHONDRIAL MARKERS IMPROVE THE PHYLOGENY OF THE HAWKSBILL TURTLE Eretmochelys imbricata (TESTUDINES: CHELONIIDAE)","authors":"G. C. Beltrán-Torres, J. Hernández-Fernández","doi":"10.35407/bag.2018.29.02.03","DOIUrl":"https://doi.org/10.35407/bag.2018.29.02.03","url":null,"abstract":"The sea turtles (Cheloniidae) are a group of seven species of cretaceous origin. Analyses of partial mitochondrial sequences have revealed phylogenetic inconsistences within this group. Nevertheless, these mitochondrial markers have allowed us to understand, explain and clarify population composition in areas of foraging, reproductive habits, inferences of migration patterns and, also, to define management units in the world, in order to trace conservation and monitoring plans. In this study, four methods were evaluated and compared for phylogenetic inference (Neighbor-Joining-NJ, Maximum Likelihood-ML, Maximum Parsimony-MP and Bayesian inference-BI) by using coding genes, ribosomal genes and full mitogenomes of the hawksbill, E. imbricata, and other six species of sea turtles obtained from GenBank. The sequences were analyzed independently and jointly to identify the method and marker that better explain the phylogenetic relationships among this group of reptiles. The NJ, ML, MP and BI trees showed that ND2, COX1, 16S rRNA, ND5, 12S rRNA, ND4 and COX3 are the markers that give phylogenetic trees with better resolution and support, with bootstrap values ranging from 89.0% to 99.98%. ATP6, ATP8, COX2, ND1, ND3, ND5 and ND4L genes presented polytomies. The analysis with full mitogenome often provides highly supported trees (bootstrap 98.0%) compared with single marker analysis. Trees obtained with the BI method and the ND2 gene is the one that better resolved the evolutionary relationships among the species, consolidating the position of E. imbricata within the Carettini tribe with a value of posterior probability of 0.98-1.0. The markers ND2, ND4, ND5 and COIII, not used in previous works, represent a new alternative to explain the phylogeny in this group of marine reptiles. In the present study, a complete mitogenome analysis produced robust and highly supported trees.\u0000\u0000Key words: phylogenetic relationships, Eretmochelys imbricate, mitogenoma, sea turtles, bootstrap, polytomies","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"219 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114993326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POLLEN VIABILITY AND MEIOTIC IRREGULARITIES IN A COLLECTION OF Stevia rebaudiana (Bertoni) Bertoni CULTIVATED IN TUCUMÁN, ARGENTINA 阿根廷TUCUMÁN栽培甜菊菊花粉活力和减数分裂不规则性研究

Journal of Basic and Applied Genetics Pub Date : 2018-12-01 DOI: 10.35407/bag.2018.29.02.05

{"title":"POLLEN VIABILITY AND MEIOTIC IRREGULARITIES IN A COLLECTION OF Stevia rebaudiana (Bertoni) Bertoni CULTIVATED IN TUCUMÁN, ARGENTINA","authors":"","doi":"10.35407/bag.2018.29.02.05","DOIUrl":"https://doi.org/10.35407/bag.2018.29.02.05","url":null,"abstract":"Stevia rebaudiana (Bertoni) Bertoni (2n=2x=22) (Asteraceae family) is a species of economic value due to the presence of steviol glycosides in leaves -mainly stevioside and rebaudioside A- which are non-caloric sugars. In 2013, a collection of plants (genotypes) from four contrasting environments was established in Tucumán, Argentina, for evaluation under local conditions and, eventually, breeding purposes. As a first step, pollen viability and meiosis was studied in a sample of 56 plants. The percentage of pollen viability varied from medium (69.4%) to high (99.6%) in 52 of them, but from low (36.5%) to relatively low (51.5%) in the remaining four. The latter four plants also presented pollen grains of heterogeneous size, which were classified as n (normal, the most frequent size in the sample), n. Abnormalities were observed in meiosis and at the tetrad stage. Pollen viability appears not to be a problem for planning crossing experiments within the collection. Moreover, the observation of parallel spindles at Anaphase II and dyads and triads at the tetrad stage entails the possibility of eventually exploring ploidy manipulations in breeding.\u0000\u0000Key words: Stevia, pollen viability, meiosis, sporads","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"46 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132532720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARISON OF THE BLUP PREDICTION ORDER AND THE RESPONSE TO SELECTION IN RABBITS WHEN THE EFFECT YEAR-SEASON IS CONSIDERED TO BE EITHER FIXED OR RANDOM

Journal of Basic and Applied Genetics Pub Date : 2018-12-01 DOI: 10.35407/bag.2018.29.02.02

E. N. Fernandez, N. Abbiati, M.S. Rovegno, C. P. González, R. Martínez

{"title":"COMPARISON OF THE BLUP PREDICTION ORDER AND THE RESPONSE TO SELECTION IN RABBITS WHEN THE EFFECT YEAR-SEASON IS CONSIDERED TO BE EITHER FIXED OR RANDOM","authors":"E. N. Fernandez, N. Abbiati, M.S. Rovegno, C. P. González, R. Martínez","doi":"10.35407/bag.2018.29.02.02","DOIUrl":"https://doi.org/10.35407/bag.2018.29.02.02","url":null,"abstract":"In close reproductive lines of rabbits reared in discrete generations, there is a strong association between the year-season (AE) and the levels of consanguinity (F). Previous studies have shown that when AE and F were considered as fixed effects in a model, the heritability and the genetic tendency were overestimated. The objective of this work was to investigate the consequences of considering AE as either a fixed (M1) or random (M2) effect on the selection of animals. A total of 15,671 records corresponding to the number of rabbits weaned in line A, from the Department of Animal Science of the Polytechnic University of Valencia, Spain, were used. The comparison of models was based on the rankings of the estimated additive genetic values in each generation and the expected response to the selection. Six scenarios were proposed, varying in selection intensities. In each one, the Spearman correlation was estimated and the percentage of discordance (D) between the animals chosen for M2 but not for M1 was calculated. The response to selection for the animals chosen by either M1 or M2, was estimated as the slope of the regression line between the means of the genetic values predicted by M2 through generations. As the selection pressure decreased, the Spearman correlations increased, decreasing the D. However, at the generation level, both estimators did not always maintain that relationship. The estimated responses to selection were similar when the selection was based on estimated breeding values obtained by using either M1 or M2. Therefore, no differences are expected between both models with respect to selection.\u0000\u0000Key words: mixed models, Spearman’s correlation, genetic trend, rabbits","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125906104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GM. GENÉTICA MÉDICA 转基因医学遗传学

Journal of Basic and Applied Genetics Pub Date : 2012-10-01 DOI: 10.35407/bag.2019.xxx.01.suppl.11

引用次数: 0

ÉTICA EN LAS PUBLICACIONES CIENTÍFICAS 科学出版物中的伦理

Journal of Basic and Applied Genetics Pub Date : 2011-11-01 DOI: 10.3305/NH.2011.26.6.4453

E. Arribalzaga

{"title":"ÉTICA EN LAS PUBLICACIONES CIENTÍFICAS","authors":"E. Arribalzaga","doi":"10.3305/NH.2011.26.6.4453","DOIUrl":"https://doi.org/10.3305/NH.2011.26.6.4453","url":null,"abstract":"En los procesos de revisión de manuscritos se plantean cuestiones éticas y legales, las que se han ahondado con el acceso masivo a internet y el incremento constante y significativo del número de publicaciones electrónicas. Algunas de estas cuestiones pueden deberse al desconocimiento por parte de los autores de lo que entraña la propiedad intelectual (derechos de autor o copyright) y de los consensos mínimos alcanzados por la comunidad científica sobre ética en las publicaciones de este tipo. Ese desconocimiento puede conducir a situaciones indeseables, que pueden llegar hasta el rechazo de manuscritos como único motivo.\u0000\u0000Para mantener la calidad académica de las revistas científicas, desde hace varios años las editoriales de prestigio aplican herramientas para la detección de plagio. Más recientemente, el uso de dichas herramientas (u otra forma de detección de plagio) forma parte de los protocolos que deben cumplimentar las revistas científicas de acceso abierto (Open Access Journals= OAJ), para la acreditación por parte de instituciones u organizaciones científicas nacionales o extranjeras. Por eso, el objetivo de esta nota es brindar algunas definiciones, reglas y sugerencias para evitar cuestiones éticas y/o legales indeseables en la elaboración de manuscritos para publicación en revistas científicas en general.","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123539697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0