Journal of Basic and Applied Genetics最新文献

EXPANSION OF THE BASIC KNOWLEDGE ON THE INHERITANCE OF CHARACTERS THAT ALLOW THE DEVELOPMENT OF NEW MAIZE BREEDING TECHNIQUES 扩展性状遗传的基本知识，使新的玉米育种技术得以发展

Journal of Basic and Applied Genetics Pub Date : 2023-07-01 DOI: 10.35407/bag.2023.34.01.03

J. C. Salerno, M. Kandús, A. Prada, D. Almorza

{"title":"EXPANSION OF THE BASIC KNOWLEDGE ON THE INHERITANCE OF CHARACTERS THAT ALLOW THE DEVELOPMENT OF NEW MAIZE BREEDING TECHNIQUES","authors":"J. C. Salerno, M. Kandús, A. Prada, D. Almorza","doi":"10.35407/bag.2023.34.01.03","DOIUrl":"https://doi.org/10.35407/bag.2023.34.01.03","url":null,"abstract":"In order to increase the efficiency of maize hybrid seed production it is necessary to achieve a high grain yield to reduce production costs. This goal requires an expansion of the basic knowledge of the inheritance of characters in order to develop new breeding techniques to improve experimental materials with hard endosperm (flint). The balanced lethal system allows to study the relative contribution of different chromosome segments to hybrid vigour due to the heterozygosity of certain chromosome segments while the rest of the genome becomes homocygotic through continuous selfing. In this way, these segments can be transferred to inbred lines in order to increase grain yield or tassel size (to increase pollen production). The goal of this study was to transfer a heterotic segment by using a balanced lethal system regulated line (BLS14), through crosses and backcrosses, to S5 flint lines derived from two commercial hybrids, ACA 2000 and Cóndor with closed pedigree, with the objective of increase grain yield or tassel size for pollen production. The analysis of variance (ANOVA) and principal components analysis (PCA) showed a significant improvement in grain yield and tassel size in the S5 flint lines of both commercial hybrids, carrying the heterotic segment of the BLS14 line.\u0000\u0000Key words: maize, heterotic segments, grain yield, inbred lines","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116548992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE GENETIC IMPROVEMENT OF PRAIRIE GRASS (Bromus catharticus Vahl) IN ARGENTINA: SYNTHESIS OF ACHIEVEMENTS AND ADVANCES 阿根廷草原草(Bromus catharticus Vahl)遗传改良:成果与进展的综合

Journal of Basic and Applied Genetics Pub Date : 2023-07-01 DOI: 10.35407/bag.2023.34.01.05

Pergamino Martín, Fierro Mag, Inta Calvu, Martín Fierro

引用次数: 0

THE IMPORTANCE OF DEEP GENOTYPING IN CROP BREEDING 深层基因分型在作物育种中的重要性

Journal of Basic and Applied Genetics Pub Date : 2023-07-01 DOI: 10.35407/bag.2023.34.01.02

A. Zambelli

引用次数: 0

ALT-EJ GENERA REARREGLOS CROMOSÓMICOS EN RESPUESTA A ETOPÓSIDO EN CÉLULAS HUMANAS CON LOS PRINCIPALES SISTEMAS DE REPARACIÓN DE RUPTURAS DE DOBLE CADENA COMPROMETIDOS ALT-EJ在人类细胞中对依托泊苷产生染色体重排，主要的双链断裂修复系统受损

Journal of Basic and Applied Genetics Pub Date : 2023-07-01 DOI: 10.35407/bag.2023.34.01.04

J. Kramar, M. Palmitelli, M. De Campos-Nebel, M. González-Cid

{"title":"ALT-EJ GENERA REARREGLOS CROMOSÓMICOS EN RESPUESTA A ETOPÓSIDO EN CÉLULAS HUMANAS CON LOS PRINCIPALES SISTEMAS DE REPARACIÓN DE RUPTURAS DE DOBLE CADENA COMPROMETIDOS","authors":"J. Kramar, M. Palmitelli, M. De Campos-Nebel, M. González-Cid","doi":"10.35407/bag.2023.34.01.04","DOIUrl":"https://doi.org/10.35407/bag.2023.34.01.04","url":null,"abstract":"The antitumor drug Etoposide (ETO) induces DNA double-strand breaks (DSB) and is associated with the development of secondary neoplasms in treated patients. DSB are repaired by two main mechanisms, homologous recombination (HR) and classical non-homologous end joining (c-NHEJ). When HR and c-NHEJ are defective, DSB are repaired by the PARP-1-dependent alternative end-joining (alt-EJ) pathway. The involvement of alt-EJ in the progression of DSB induced by ETO in the G2 phase of human cells was analyzed. HeLa cells deficient in HR (cohesin RAD21 inhibition, HeLa RAD21kd) and their non-silencing control (HeLa NS) were established. Cells were treated with ETO in the presence of a chemical inhibitor of DNA-PKcs (DNA-PKi, c-NHEJ). In both cell lines, ETO-induced DSB (γH2AX+) in G2 phase were increased compared to their controls. The incorrect repair of DSB in DNA-PKcs- and RAD21-deficient cells caused a synergistic augment in chromatid exchanges and dicentric chromosomes in the first and second metaphase, respectively. In contrast, the frequency of dicentric chromosomes was reduced in PARP-1-deficient cells (HeLa PARP-1kd) following ETO treatment. In HeLa RAD21kd binucleated cells, DNA-PKi/ETO increased the percentage of cells with ≥20 γH2AX foci in the G1-postmitotic phase and of micronuclei at 96 h. A greater accumulation in G2/M was observed in HeLa NS treated with DNA-PKi/ETO compared with HeLa RAD21kd at 8 h. The cell cycle restarted in HeLa NS at 16 h; however, the G2/M accumulation was maintained in HeLa RAD21kd. Chromosomal rearrangements obtained when DNA-PKcs and RAD21 were absent and their decrease in HeLa PARP-1kd cells suggest that alt-EJ contributes to their formation.\u0000\u0000Key words: chromosomal aberrations, cell cycle, cohesin, double-strand breaks, DNA repair pathways","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125508913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HISTORY AND PROSPECTS OF FODDER CORN BREEDING IN ARGENTINA 阿根廷饲料玉米育种的历史与展望

Journal of Basic and Applied Genetics Pub Date : 2023-07-01 DOI: 10.35407/bag.2023.34.01.06

P. Rimieri

{"title":"HISTORY AND PROSPECTS OF FODDER CORN BREEDING IN ARGENTINA","authors":"P. Rimieri","doi":"10.35407/bag.2023.34.01.06","DOIUrl":"https://doi.org/10.35407/bag.2023.34.01.06","url":null,"abstract":"Corn is used to massively produce food for humans and domestic animals with grains of various taxonomic entities or races. For domestic ruminants, the whole plant is also used as forage. In Argentina, both corn grain and whole-plant silage are used for beef and dairy cattle production. This paper aimed to develop the history and perspectives of corn grown for grain and silage, focusing on plant breeding. The importance of corn fodder in its two variants (grain and whole-plant silage) has varied over time. We emphasize herein the importance of the specific genetic breeding of corn used for whole-plant silage to achieve higher yield potential and nutritional value than grain hybrids. The four population genetic structures used over time as cultivars, which determined the evolution of the technological process of selection and breeding, were analyzed. Based on the research carried out, seed companies have incorporated our protocols into their development and breeding programs. The contribution of selection and breeding in Argentina was effective in transforming the corn plant into ruminant feed, and this will increase with the development of specific silage hybrids.\u0000\u0000Key words: cultivars, corn fodder, plant breeding","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116408517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CLONAL DETECTION OF Streptococcus agalactiae Lehmann AND Neumann PARENTAL STRAINS BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA 随机扩增多态DNA检测无乳链球菌莱曼和诺伊曼亲本菌株

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.04

Cortese, I.J., Novosak M.G., Oviedo P.N., Cannistraci Giolito R.E., M. Laczeski

{"title":"CLONAL DETECTION OF Streptococcus agalactiae Lehmann AND Neumann PARENTAL STRAINS BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA","authors":"Cortese, I.J., Novosak M.G., Oviedo P.N., Cannistraci Giolito R.E., M. Laczeski","doi":"10.35407/bag.2022.33.02.04","DOIUrl":"https://doi.org/10.35407/bag.2022.33.02.04","url":null,"abstract":"Streptococcus agalactiae (GBS) causes invasive infections in newborns, being the most frequent the maternal transmission. Epidemiological studies use molecular techniques that assess genetic diversity, including random amplification of polymorphic DNA (RAPD) that is found to be accessible, sensitive and uses arbitrary primers to amplify polymorphic segments of DNA by PCR. The objective was to determine the clonal relationship between GBS strains recovered from mothers and their respective newborns. Four pairs of GBS isolates obtained from vaginal-rectal swabs of mothers and blood cultures of their newborns were studied with RAPD. Primers OPS11, OPB17 and OPB18 were used to select one with the ability to discriminate between non-genetically related strains. The Hunter-Gaston formula that establishes the discrimination index (D) was used; when D>0.90, it is considered that the isolates belong to different clones. The amplification profiles for the eight isolates, using each primer independently, allowed to calculate a D=1 for OPS11, and D=0.84 for OPB17 and OPB18. Therefore, OPS11 was selected for the study of the clonal relationship of the isolates, and similar amplification profiles were found by RAPD for each mother-newborn pair of GBS isolates. Different amplification profiles were observed between pairs of mother-newborn strains, which reveals the discrimination between unrelated strains, confirmed by pulsating field electrophoresis (PFGE). These results indicated vertical transmission for each studied case and robustness of the OPS11 primer. Appropriate conditions of the RAPD trial were found, which is useful for epidemiological studies.\u0000\u0000Key words: Streptococcus agalactiae, neonatal disease, molecular epidemiology, RAPD technique, vertical transmission","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128132920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMUNICACIONES LIBRES 通信自由

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.01.suppl.02

André DE Oliveira Baldoni, Anna Paula, DE SÁ Borges, Leonardo Régis Leira Pereira

引用次数: 0

KNOWLEDGE ABOUT GENETICS AND TRUST IN GENETIC TESTING IN A MID-SIZE CITY IN ARGENTINA 阿根廷一个中型城市的遗传学知识和对基因检测的信任

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.03

M. Mendoza, B. Mazza, G. S. Cabana, L. Smith, F. Di Fabio Rocca, H. Delfino, C. Martínez

{"title":"KNOWLEDGE ABOUT GENETICS AND TRUST IN GENETIC TESTING IN A MID-SIZE CITY IN ARGENTINA","authors":"M. Mendoza, B. Mazza, G. S. Cabana, L. Smith, F. Di Fabio Rocca, H. Delfino, C. Martínez","doi":"10.35407/bag.2022.33.02.03","DOIUrl":"https://doi.org/10.35407/bag.2022.33.02.03","url":null,"abstract":"Public attitudes about genetics appear to depend on the local context. We analyzed survey responses obtained in 2015 from 293 residents of Luján, a city in the province of Buenos Aires, Argentina, who self-assessed their knowledge about genetics and their trust in genetic tests. The survey integrated a larger research project for which consenting adult participants shared demographic and genealogical information and provided saliva samples for genetic ancestry analyses. Participants reported little knowledge but high trust in genetic testing when questioned about knowledge and trust. Well-known media stories of DNA-based forensic genetic investigations to identify the victims of state repression during the military dictatorship may have contributed to the high self-assessment of their genetic knowledge expressed by some participants, regardless of educational attainment. Our analysis provides information that could be used as a baseline to begin unraveling the current level of public trust in genetics in a region of the Global South where genetic testing has become widespread, but people’s knowledge of and trust in genetics remain poorly studied.\u0000\u0000Key words: genetic tests, knowledge, public attitudes, trust","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132565497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IMPACT OF GENETIC ANCESTRY ON THE DISTRIBUTION OF INTERFERON-λ4 RS12979860 POLYMORPHISM IN A GLOBAL POPULATION OF BUENOS AIRES, ARGENTINA 遗传祖先对阿根廷布宜诺斯艾利斯全球人群干扰素-λ4 rs12979860多态性分布的影响

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.02

F. Mansilla, S. Avena, C. Dejean, C. Turco, A. Capozzo

{"title":"IMPACT OF GENETIC ANCESTRY ON THE DISTRIBUTION OF INTERFERON-λ4 RS12979860 POLYMORPHISM IN A GLOBAL POPULATION OF BUENOS AIRES, ARGENTINA","authors":"F. Mansilla, S. Avena, C. Dejean, C. Turco, A. Capozzo","doi":"10.35407/bag.2022.33.02.02","DOIUrl":"https://doi.org/10.35407/bag.2022.33.02.02","url":null,"abstract":"Human interferon-λ4 is a cytokine involved in early stages of antiviral responses. Strikingly, some allelic variants with diminished antiviral activity reduce the susceptibility to viral infections, thus they would have suffered a positive selection pressure throughout the evolutionary history of the genus Homo. An intronic variant within the IFNλ4 locus (rs12979860, T˃C) emerged as one of the main gene determinants of the response to HCV and other viruses. The rs12979860-C allele has a differential frequency in African, European and Native American populations, though South American data are scarce. Here we characterize for the first time the distribution of rs12979860 genotypes in a sample of the global population of Buenos Aires, Argentina, assessing its association with European, Native American and African parental components. The rs12979860 genotypes were determined by PCR-RFLP in DNA samples from donors of a blood banks of Buenos Aires (n=96), whose genetic individual ancestry (European, African or Native American) had been previously determined using molecular markers. The distribution of rs12979860-CC, CT and TT was 29.17%, 50.0% and 20.83%, respectively. A significant increase in the frequency of CC among donors with a strong European contribution and a greater impact of the Native American component among donors carrying the T allele were observed. Native American and European components were associated to the rs12979860 distribution in a sample of the global population of Buenos Aires, while no differences were directly attributable to the African ancestry. Considering interferon´s key role in antiviral responses, our results may contribute to both bioanthropological and immunogenetic studies associated with infectious diseases.\u0000\u0000Key words: ancestry, Buenos Aires, IFNλ4 polymorphism, rs12979860 distribution.","PeriodicalId":124759,"journal":{"name":"Journal of Basic and Applied Genetics","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127065427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SELECCIÓN CONTRA DISPLASIA DE CADERA CANINA EN EL OVEJERO ALEMÁN 德国牧羊犬对犬髋关节发育不良的选择

Journal of Basic and Applied Genetics Pub Date : 2022-12-01 DOI: 10.35407/bag.2022.33.02.05

M. Poverene

引用次数: 0