J. Daviña, A. C. Gianini Aquino, O.A. Rodríguez Mata, E. Tapia-Campos, R. Barba-González, A. Honfi
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引用次数: 0
摘要
西风菊是一种观赏美洲的球茎植物，因其黄色的花萼具有美观的品质而被用作观赏花园作物。本工作的目的是通过经典染色体染色和荧光带来表征该物种。孢子体染色体数目为2n=8x=48条染色体，核型公式为20 m + 26 sm + 2 st. 8、9、11和12号染色体短臂上检测到卫星染色体，与组成型异染色质CMA+/DAPI-/0共定位。核型包括末端组成异染色质带的染色体对，包括卫星和异型簇，表明它是异位倍体。这些结果将用作监测遗传改进的工具，用于种间杂交及其后代，以及通过体外培养的生物技术程序。关键词:组成异染色质，染色体显带，球茎，植物遗传资源，核型
CHROMOSOMIC STUDIES IN ZEPHYRANTHES CITRINA BAKER (AMARYLLIDACEAE), A POLYPLOID ORNAMENTAL
Zephyranthes citrina is an ornamental American bulbous plant used as an ornamental garden crop for the aesthetic qualities of its yellow perigonium. The objective of this work was to characterize the species by classical chromosome staining and fluorochrome banding. A sporophytic chromosome number of 2n=8x=48 chromosomes was observed, being the karyotypic formula 20 m + 26 sm + 2 st. Satellites were detected in the short arm of metacentric chromosomes 8, 9, 11 and 12, which colocalized with constitutive heterochromatin CMA+/DAPI-/0 bands. The karyotype comprised chromosome pairs with terminal constitutive heterochromatin bands that included satellites and heteromorphic clusters indicating that it is an allooctoploid. These results will be used as a tool for monitoring genetic improvement, in interspecific crosses and its progenies and in biotechnological procedures by in vitro culture.
Key words: constitutive heterochromatin, chromosome banding, bulbous, plant genetic resources, karyotype
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