Frontiers in Cellular and Infection Microbiology最新文献

{"title":"Alterations of the salivary microbiome in obstructive sleep apnea and their association with periodontitis.","authors":"Jiong Li, Jike Gao, Yunxia Ma, Wuli Li, Xiangru Chen, Zhenhua Li, Xiujun Zhang","doi":"10.3389/fcimb.2025.1642766","DOIUrl":"10.3389/fcimb.2025.1642766","url":null,"abstract":"<p><strong>Objective: </strong>Obstructive sleep apnea (OSA) and periodontitis have demonstrated epidemiological and clinical associations. This study aimed to characterize salivary microbiome alterations in patients with OSA, periodontitis, and their comorbidity (OSA+PD), and to explore potential microbial markers.</p><p><strong>Materials and methods: </strong>This cross-sectional study included 125 adults divided into four groups: healthy controls (H, n=26), patients with OSA (OSA/O, n=42), patients with periodontitis (PD/P, n=15), and patients with OSA and periodontitis (OSA+PD/OP, n=42). Participants underwent nocturnal polysomnography and comprehensive periodontal examinations. Saliva samples were collected and analyzed using 16S ribosomal DNA gene sequencing to evaluate microbial distribution and community structure across groups. Receiver operating characteristic (ROC) curves were generated for key taxa combining with clinical indicators, and the area under the curve (AUC) values were calculated to assess diagnostic relevance.</p><p><strong>Results: </strong>Oral microbial diversity was significantly altered in OSA, PD, and OSA+PD groups. Alpha diversity was reduced in all patient groups compared to healthy controls, with the periodontitis group showing the highest diversity and evenness. Beta diversity revealed that periodontitis having the strongest impact and the comorbid group exhibiting intermediate characteristics between OSA and periodontitis. Key taxa, including <i>Tannerella</i>, <i>Treponema</i>, <i>Prevotella</i>, <i>Slackia</i>, and <i>Streptococcus constellatus</i>, exhibited significant intergroup differences. BugBase phenotype analysis revealed an increased abundance of aerobic and a reduced presence of anaerobic microbial profiles in the OSA and OSA+PD groups. Additionally, <i>Rothia</i> and <i>Micrococcaceae</i> were more abundant in the OSA group, regardless of periodontal status. Receiver operating characteristic (ROC) analysis indicated that <i>Rothia</i> and <i>Parvimonas</i> reliably differentiated between OSA and OSA+PD (AUC=0.715, 0.702) and also between periodontitis and OSA+PD (<i>Rothia</i>: AUC=0.879).</p><p><strong>Conclusion: </strong>OSA is associated with distinct changes in salivary microbiota, including reduced microbial richness and altered functional profiles, which may contribute to early periodontal dysbiosis. <i>Rothia</i> has been identified as a potential microbial biomarker for OSA-related periodontitis, while <i>Rothia</i> and <i>Parvimonas</i> may play a key role in periodontitis-related OSA. However, as this is a cross-sectional study, causal relationships and the predictive value of microbial biomarkers remain to be confirmed in longitudinal studies. These results highlight the need for integrated management of OSA and periodontitis and suggest microbial profiling as a useful diagnostic tool.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1642766"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The current epidemic status and prevention and control of bovine viral diarrhea virus in yaks in China.","authors":"Zhi Li, Yuan Han, Qing Yuan, Shuqin Wang, Changjiang Chen, Shandian Gao, Ru Meng","doi":"10.3389/fcimb.2025.1647328","DOIUrl":"10.3389/fcimb.2025.1647328","url":null,"abstract":"<p><p>In China, yaks are predominantly distributed across the Qinghai-Tibet Plateau and surrounding high-altitude regions, including Tibet, Qinghai, Sichuan, Gansu, Xinjiang and Yunnan. These animals serve as multifunctional resources for local herders, providing meat, dairy, hides, and wool, while also constituting a critical component of the industrial chain in high-altitude ecosystems. Recent epidemiological studies have demonstrated an increasing trend in bovine viral diarrhea virus (BVDV) infection rates among yak populations in provinces such as Gansu, Sichuan, Tibet, and Qinghai. This review not only summarizes the epidemiological status, distribution of viral sub-genotypes, and current prevention and control in yaks across various regions, but also proposes, for the first time, a systematic \"Five-dimensional Integration\" comprehensive prevention and control model for BVDV, including vaccine breakthrough, precise monitoring, dynamic early warning, population purification, and active prevention, which will provide directed insight for the prevention and control strategies of yaks infected by BVDV.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1647328"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of antibiotic choice on immune response and antibiotic resistance development in piglets experimentally infected with <i>Escherichia coli</i>.","authors":"Leon Cantas, Christopher G Fenton, Emese Bato, Ruth H Paulssen, Henning Sørum","doi":"10.3389/fcimb.2025.1627782","DOIUrl":"10.3389/fcimb.2025.1627782","url":null,"abstract":"<p><p>The rapid mobility of antibiotic resistance genes (ARGs) represents a growing global One Health concern. However, further <i>in vivo</i> studies are needed to better understand ARG dissemination in actual clinical settings. To this end, a piglet model of enteric colibacillosis with the causative bacterium carrying an R-plasmid, was used to track the expression of genes involved in the bacterial SOS response, plasmid transfer, and porcine immune responses under both effective and ineffective antibiotic treatments. Analysis of gut samples showed a significant reduction (<i>p</i> < 0.05) in the expression of R-plasmid transfer genes in groups receiving effective enrofloxacin, with or without probiotics or meloxicam. Conversely, ineffective tetracycline and sub-inhibitory enrofloxacin resulted in a significant increase (<i>p</i> < 0.05) in the expression of bacterial SOS response and R-plasmid transfer genes. Inflammatory gene expression was upregulated in the groups receiving ineffective antimicrobial treatment, whereas anti-inflammatory cytokines exhibited the opposite trend in effectively treated piglets. These findings highlight the importance of selecting the correct antibiotic and administering it at an effective dosage. The improper use of antibiotics or their administration at subinhibitory concentrations can result in high mortality/morbidity rates and accelerate the spread of ARGs.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1627782"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The role of microbial glycosylation in host-pathogen interactions.","authors":"Kelly M Fulton, Susan M Twine, Christopher W Reid","doi":"10.3389/fcimb.2025.1689780","DOIUrl":"10.3389/fcimb.2025.1689780","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1689780"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological subtypes and sampling strategies determine diagnostic sensitivity in cervical lymph node tuberculosis: a retrospective study.","authors":"Xiaoyu Liu, Xuan Wang, Yuejie Li, Qibin Liu, Chao Quan, Xiyong Dai","doi":"10.3389/fcimb.2025.1662518","DOIUrl":"10.3389/fcimb.2025.1662518","url":null,"abstract":"<p><strong>Objective: </strong>To investigate how pathological types and sampling methods affect positivity rates of five diagnostic techniques in cervical lymph node tuberculosis.</p><p><strong>Methods: </strong>We retrospectively analyzed 198 surgically confirmed cervical lymph node tuberculosis patients from Wuhan Pulmonary Hospital. Cases were stratified by pathological subtypes and collection methods. The specimens were tested using acid-fast bacillus smear microscopy, mycobacterium tuberculosis culture, quantitative polymerase chain reaction for tuberculosis DNA, simultaneous amplification and testing for tuberculosis, or GeneXpert.</p><p><strong>Results: </strong>All 198 cases showed granulomatous inflammation. Liquefactive necrosis occurred in 91.92% (182/198) of cases, with caseous necrosis in 87.88% (174/198), adjacent soft-tissue necrosis in 57.07% (113/198), and suppurative inflammation in 20.20% (40/198). Solid alterations without liquefactive necrosis (coagulative necrosis/non-necrotizing lymphadenitis) comprised 8.08% (16/198). The overall etiological positivity rate was 90.40% (179/198). GeneXpert showed highest sensitivity (90.36%), followed by tuberculosis DNA (74.24%), simultaneous amplification and testing (40.22%), Mycobacterium tuberculosis culture (16.67%), and acid-fast bacillus smear (14.72%). Among 33 culture-positive cases, 32 (96.97%) were GeneXpert positive. Rifampicin resistance detected by GeneXpert was 5.62% (10/178). In specimens with caseous necrosis, soft-tissue necrosis, or liquefactive necrosis, GeneXpert positivity significantly exceeded tuberculosis DNA (all P < 0.01). Liquefactive necrosis samples showed higher positivity than solid-change specimens for all techniques except culture (all P < 0.001). Drainage specimens yielded higher tuberculosis DNA and GeneXpert positivity than surgical resection specimens. Combining surgical and drainage specimens increased culture positivity to 26.09%.</p><p><strong>Conclusion: </strong>Etiological positivity rates in cervical lymph node tuberculosis correlate with pathological features. Maximizing liquefactive necrosis sampling for the GeneXpert assay and combining different sampling techniques (such as, surgical resection, incision and drainage, needle biopsy) for etiological detection enhances diagnostic accuracy.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1662518"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: The impact of pharmacokinetics and pharmacodynamics on antimicrobial stewardship.","authors":"Zeynep Ture, Emine Alp Meşe, Joana Alves, Claire Roger, Rony M Zeenny","doi":"10.3389/fcimb.2025.1684355","DOIUrl":"10.3389/fcimb.2025.1684355","url":null,"abstract":"","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1684355"},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between gut microbiota and anesthesia: mechanism exploration and translation challenges focusing on the gut-brain-liver axis.","authors":"Ruolan Zhang, Li Li, Gaojie Yu, Yang Li, Kexi Wei, Liang Lin, Yifeng Ye","doi":"10.3389/fcimb.2025.1626585","DOIUrl":"10.3389/fcimb.2025.1626585","url":null,"abstract":"<p><p>As a core participant in human metabolism, immunity, and neural regulation, the gut microbiota has been demonstrated to be closely related to anesthesia drug metabolism and perioperative complications in recent years. Via the bidirectional interaction between the gut-brain axis and gut-liver axis, the gut microbiota and its metabolites can regulate central nervous system inflammation, liver drug-metabolizing enzyme activity, and the clearance efficiency of anesthetic drugs. Moreover, anesthetic drugs can significantly reshape the gut microbiota structure by altering intestinal barrier function, inhibiting beneficial bacterial proliferation, or inducing bile acid metabolism disorders, thereby resulting in a vicious cycle of neuroinflammation and metabolic abnormalities. Microbiota-targeted intervention strategies have demonstrated potential in alleviating anesthesia-related complications in response to this interactive network; however, their clinical translation is still limited by incomplete understanding of the underlying mechanisms, individual heterogeneity, and safety challenges. In the future, it will be necessary to integrate multiomics technologies for analyzing the tripartite interaction network of microorganisms, hosts, and drugs, as well as for promoting standardized clinical research, in order to develop individualized anesthesia management plans based on gut microbiota regulation; these initiatives can result in improvements in perioperative safety and patient prognosis.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1626585"},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of pan-azole resistance in <i>Cryptococcus neoformans</i> by agrochemical azole uniconazole through upregulation of efflux and chromosomal disomies.","authors":"Maoji Zhang, Weihua Ma, Jing Wang, Feng Yang, Jingjing Zhong, Yi Xu","doi":"10.3389/fcimb.2025.1664896","DOIUrl":"10.3389/fcimb.2025.1664896","url":null,"abstract":"<p><p><i>Cryptococcus neoformans</i> is a fungus naturally found in the environment, particularly in soil, bird droppings, and trees. Cryptococcosis, caused by <i>Cryptococcus</i> spp., primarily <i>C. neoformans</i> and <i>C. gattii</i>, poses a significant threat to human health. Agrochemicals are widely used worldwide, and most applied agrochemicals are dispersed into the environment, which can have direct and indirect effects on human health. This study investigates the impact of the plant growth regulator uniconazole (UCZ) on antifungal resistance in <i>C. neoformans</i> using the H99 laboratory strain. We found UCZ to have potent antifungal activity, and exposure to UCZ induced genomic alterations, resulting in cross-resistance to both agricultural and medical azoles. The adaptors showed altered gene expressions across the genome, including efflux genes, as well as increased efflux pump activity. Deletion and overexpression of <i>AFR1</i> demonstrated its role in mediating resistance to azoles, with unexpected effects on amphotericin B sensitivity. These findings underscore the significant impact of agricultural agrochemicals on antifungal resistance development and the importance of considering environmental exposures in resistance management strategies.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1664896"},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum ferritin as a predictive biomarker for PEG IFNα-2b efficacy in chronic hepatitis B treatment.","authors":"Meiya Chen, Chenhao Wang, Miqiang Lin, Shiran Cai","doi":"10.3389/fcimb.2025.1603286","DOIUrl":"10.3389/fcimb.2025.1603286","url":null,"abstract":"<p><strong>Background: </strong>Chronic hepatitis B (CHB) caused by HBV infection leads to persistent liver inflammation. PEG IFNα-2b shows higher functional cure rates than oral antivirals, but identifying optimal candidates remains challenging. This study evaluates serum ferritin as a predictive biomarker for PEG IFNα-2b efficacy.</p><p><strong>Methods: </strong>Serum ferritin, HBsAg and transaminase were monitored in CHB patients treated with PEG IFN α-2b at baseline (0w), 12w and 24w. Patients were divided into the effective group (HBsAg reduction ≥1 log10 IU/mL or clearance) and the ineffective group (HBsAg reduction <1 log10 IU/mL) at 24 weeks. Similarly, for the results at 48 weeks, the patients were divided into the clearance group (achieving HBsAg clearance) and the non-clearance group (not achieving HBsAg clearance). To analyze the correlation between serum ferritin and the decrease of HBsAg, a multivariate logistic regression model was constructed. Potential confounding factors such as age, gender, HBeAg and HBV DNA status, ALT, AST, and iron metabolism indicators (FE, TIBC, TS) were included to evaluate the independent predictive effect of ferritin levels on the therapeutic effectiveness, calculate the corrected OR value and 95% CI. The predictive effect was evaluated by the ROC curve.</p><p><strong>Results: </strong>Among patients with chronic hepatitis B (CHB) who completed the PEG IFNα-2b course of treatment, the serum ferritin levels of all patients showed an increasing trend at baseline and during the treatment process, and the serum ferritin level in the effective treatment group (HBsAg reduction ≥1 log10 IU/mL or clearance) was significantly higher than that in the ineffective treatment group. It was confirmed by Spearman's rank correlation analysis that the serum ferritin level was positively correlated with the decrease in HBsAg. Further analysis through the multivariate logistic regression model revealed that serum ferritin remained an independent predictor of the antiviral efficacy of PEG IFNα-2b. ROC curve analysis indicated that serum ferritin had a high predictive value for the antiviral efficacy of PEG IFNα-2b.</p><p><strong>Conclusion: </strong>The level of serum ferritin is closely related to the anti-hepatitis B virus efficacy of PEG IFNα-2b. It has great potential as a reliable and economical biomarker to guide treatment and optimize treatment strategies.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1603286"},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caspofungin paradoxical growth in <i>Candida albicans</i> requires stress pathway activation and promotes unstable echinocandin resistance mediated by aneuploidy.","authors":"Ying Wei, Jing Wang, Nan Tang, Ziwei Lin, Wenhui Li, Yi Xu, Liangsheng Guo","doi":"10.3389/fcimb.2025.1618815","DOIUrl":"10.3389/fcimb.2025.1618815","url":null,"abstract":"<p><p>Paradoxical growth (PG) is a counterintuitive phenomenon in which otherwise susceptible fungal cells resume proliferation at supra-MIC concentrations of echinocandins, thereby undermining the efficacy of these frontline antifungals. Despite its clinical significance, the genetic basis of PG remains poorly understood. Here, we systematically dissect the roles of key stress response pathways-Hsp90, PKC, calcineurin, and TOR-in mediating caspofungin (CSP)-induced PG in <i>Candida albicans</i> and uncover a novel genetic mechanism involving segmental aneuploidy. Disruption of these pathways via pharmacological inhibition or targeted gene deletion abolished PG, confirming their essential roles in mediating adaptive stress responses. Whole-genome sequencing of CSP-tolerant isolates revealed a recurrent segmental monosomy on the right arm of Chromosome R (SegChrRx1). Phenotypic reversion analyses demonstrated that CSP resistance is reversible and directly linked to the presence of this aneuploidy. Transcriptomic profiling of SegChrRx1 strains showed broad transcriptional remodeling, including upregulation of <i>GSC1</i> (encoding β-1,3-glucan synthase), <i>CHS3</i> and <i>CHS4</i> (chitin synthases), and key regulators of the PKC and calcineurin pathways, alongside downregulation of dosage-sensitive genes whose deletion enhances CSP resistance. Collectively, our findings reveal a dual mechanism of PG: activation of stress response pathways confers initial survival, while segmental aneuploidy enables reversible transcriptional reprogramming that promotes drug resistance. This study establishes segmental aneuploidy as a dynamic and previously underappreciated mechanism of echinocandin adaptation in <i>C. albicans</i>, with important implications for antifungal therapy.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1618815"},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}