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Mint (Mentha spp.) Honey: Analysis of the Phenolic Profile and Antioxidant Activity. 薄荷蜂蜜:酚类成分和抗氧化活性分析。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-12-01 DOI: 10.17113/ftb.60.04.22.7703
Tomislav Pavlešić, Sanja Poljak, Dijana Mišetić Ostojić, Ivana Lučin, Christian A Reynolds, Daniela Kalafatovic, Lara Saftić Martinović
{"title":"Mint (<i>Mentha</i> spp.) Honey: Analysis of the Phenolic Profile and Antioxidant Activity.","authors":"Tomislav Pavlešić,&nbsp;Sanja Poljak,&nbsp;Dijana Mišetić Ostojić,&nbsp;Ivana Lučin,&nbsp;Christian A Reynolds,&nbsp;Daniela Kalafatovic,&nbsp;Lara Saftić Martinović","doi":"10.17113/ftb.60.04.22.7703","DOIUrl":"https://doi.org/10.17113/ftb.60.04.22.7703","url":null,"abstract":"<p><strong>Research background: </strong>The composition of honey is influenced by the botanical source and geographical area of the nectar from which it is derived. Unifloral honeys reach higher market value than multifloral honeys due to their specific aromas, which result from volatile and phenolic compounds.</p><p><strong>Experimental approach: </strong>The aim of our study is to characterize the phenolic composition of a rare unifloral variety of honey - mint (<i>Mentha</i> spp.) honey. For this purpose, we performed standard physicochemical analyses, pollen analysis, determined total phenolic and flavonoid content, analysed antioxidant activity and performed qualitative and quantitative analyses of phenolic compounds for five mint honeys.</p><p><strong>Results and conclusions: </strong>Our results indicate that mint honey samples have high phenolic content, expressed in gallic acid equivalents, from (76.7±0.6) to (90.1±1.1) mg/100 g, and flavonoid content, expressed as quercetin equivalents, from (6.7±0.6) to (12.5±0.8) mg/100 g. These honey samples also exhibit strong antioxidant activity, expressed as Trolox equivalents, from (33.6±2.8) to (51.3±1.2) mg/100 g and from (14.4±0.8) to (55.1±2.4) mg/100 g when analysed with DPPH and ABTS assays, respectively. Quantitative LC-MS/MS analysis revealed that the most abundant phenols in all samples were chrysin, apigenin and <i>p</i>-coumaric acid. Qualitative LC-MS/MS analysis identified the presence of kaempferide, diosmetin, acacetin and several caffeic acid derivatives.</p><p><strong>Novelty and scientific contribution: </strong>Our study indicates that mint honey contains unique phenolic profiles, which likely contribute to its distinctive aroma and strong antioxidant activity. A detailed description of the rare honey varieties gives beekeepers greater visibility and easier access to the demanding natural product market.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 4","pages":"509-519"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10824636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits. 初级代谢物色谱分析作为浆果种子化学分类的工具。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7505
Đurđa Krstić, Tomislav Tosti, Saša Đurović, Milica Fotirić Akšić, Boban Đorđević, Dušanka Milojković-Opsenica, Filip Andrić, Jelena Trifković
{"title":"Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits.","authors":"Đurđa Krstić,&nbsp;Tomislav Tosti,&nbsp;Saša Đurović,&nbsp;Milica Fotirić Akšić,&nbsp;Boban Đorđević,&nbsp;Dušanka Milojković-Opsenica,&nbsp;Filip Andrić,&nbsp;Jelena Trifković","doi":"10.17113/ftb.60.03.22.7505","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7505","url":null,"abstract":"<p><strong>Research background: </strong>Considering the importance of consumption of berry fruits with proven health-beneficial properties and difficulties in quality control of products of specific botanical and geographic origin, a fingerprint method was developed, based on advanced data analysis (pattern recognition, classification), in order to relate the variability of nutrients in the selected cultivars to primary metabolite profile.</p><p><strong>Experimental approach: </strong>Forty-five samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry and goji berry) were characterized according to chromatographic profiles of primary metabolites (sugars, lipids and fatty acids) obtained by three chromatographic techniques (high-performance thin-layer chromatography, gas chromatography coupled to mass spectrometry, and high-performance anion-exchange chromatography with pulsed amperometric detection).</p><p><strong>Results and conclusions: </strong>Comprehensive analysis allowed monitoring and identification of metabolites belonging to polar lipids, mono-, di- and triacylglycerols, free fatty acids, free sterols, sterol esters, mono- to heptasaccharides and sugar alcohols. Chemical fingerprint of berry seeds showed the uniformity of primary metabolites within each fruit species, but revealed differences depending on the botanical origin. All three chromatographic methods provided a discriminative, informative and predictive metabolomics methodology, which proved to be useful for chemotaxonomic classification.</p><p><strong>Novelty and scientific contribution: </strong>A novel methodology for the identification of bioactive compounds from primary metabolites of natural products was described. The proposed untargeted metabolite profiling approach could be used in the future as a routine method for tracing of novel bioactive compounds. The knowledge of metabolite composition obtained in this study can provide a better assessment of genotypic and phenotypic differences between berry fruit species and varieties, and could contribute to the development of new breeding programs.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"406-417"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40448737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Lemon Juice Formulations Modulate In Vitro Digestive Recovery of Spinach Phytochemicals. 柠檬汁配方调节菠菜植物化学物质的体外消化恢复。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7104
Valerija Vujčić Bok, Ivana Šola, Gordana Rusak
{"title":"Lemon Juice Formulations Modulate <i>In Vitro</i> Digestive Recovery of Spinach Phytochemicals.","authors":"Valerija Vujčić Bok,&nbsp;Ivana Šola,&nbsp;Gordana Rusak","doi":"10.17113/ftb.60.03.22.7104","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7104","url":null,"abstract":"<p><strong>Research background: </strong><i>Citrus limon</i> (L.) Burm lemon juice is rich in many important natural chemical components (flavonoids, citric acid and vitamin C) and its use in traditional medicine is well known. Formulations of lemon juice with fruit polyphenols in beverages have been investigated, but there is very little information about their ability to modulate the digestive behaviour of polyphenols. The goal of this study is to determine the stability and digestive availability of spinach (<i>Spinacia oleracea</i> L.) polyphenols by adding different volume fractions of lemon juice (0, 2, 5, 10 and 20%) during <i>in vitro</i> digestion.</p><p><strong>Experimental approach: </strong>The content of polyphenols and other abundant compounds including nitrates, oxalic acid and l-ascorbic acid in spinach formulation with various volume fractions of lemon juice were measured in predigested and digested samples using <i>in vitro</i> human digestion model. Antioxidant and α-amylase inhibitory activities of spinach lemon juice formulation were also measured.</p><p><strong>Results and conclusions: </strong>The highest increases in total polyphenols, total flavonoids, total phenolic acids, oxalic acid and nitrate content were noted in predigested and almost all digested spinach samples formulated with the highest volume fraction of lemon juice. In the same sample, the content of individual compounds significantly increased after salivary \u2028(l-ascorbic acid), initial (<i>p</i>-coumaric acid) and intestinal (quercetin) phase of digestion. High bioaccessibility of polyphenols and l-ascorbic acid in all phases of digestion was observed in almost all spinach lemon juice formulations, with the exception of nitrates in gastric and intestinal phases and oxalic acid in the intestinal phase, which had moderate bioaccessibility.</p><p><strong>Novelty and scientific contribution: </strong>For the first time the stability and digestive availability of spinach polyphenols, oxalic acid, nitrates and l-ascorbic acid were tested with the addition of different volume fractions of lemon juice. The pH of lemon juice and its \u2028l-ascorbic acid content increase the stability and availability of polyphenols in spinach lemon juice formulation during <i>in vitro</i> digestion. Antioxidant and α-amylase inhibitory activities increase in dose-dependent manner after lemon juice addition. Accordingly, spinach formulated with 20% of lemon juice appears as the best source of dietary polyphenols with antioxidant and antidiabetic activities and nitrates that may be used as a functional drink.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"293-307"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methods of Microencapsulation of Vegetable Oil: Principles, Stability and Applications - A Minireview. 植物油微胶囊化方法:原理、稳定性及应用综述。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7329
Luana Carvalho da Silva, Rachel Menezes Castelo, Huai N Cheng, Atanu Biswas, Roselayne Ferro Furtado, Carlucio Roberto Alves
{"title":"Methods of Microencapsulation of Vegetable Oil: Principles, Stability and Applications - A Minireview.","authors":"Luana Carvalho da Silva,&nbsp;Rachel Menezes Castelo,&nbsp;Huai N Cheng,&nbsp;Atanu Biswas,&nbsp;Roselayne Ferro Furtado,&nbsp;Carlucio Roberto Alves","doi":"10.17113/ftb.60.03.22.7329","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7329","url":null,"abstract":"<p><p>In addition to being used in food, fuel and lubricants, vegetable oils are promising in many other applications such as food additives, nutritional supplements, cosmetics and biomedicine; however, their low oxidative stability can limit their use. Microencapsulation is a well-established method for the preservation of oil against degradation, controlled release of active ingredients, protection against external factors during storage, and enhanced durability. In this article, microencapsulation methods for vegetable oil are reviewed, including physical methods (spray-drying and freeze-drying), physicochemical methods (complex coacervation, ionic gelation and electrostatic layer-by-layer deposition), and chemical methods (interfacial/<i>in situ</i> polymerization). This article also provides information on the principles, parameters, advantages, disadvantages and applications of these methods.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"308-320"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Microencapsulation on Chemical Composition and Antimicrobial, Antioxidant and Cytotoxic Properties of Lemongrass (Cymbopogon flexuosus) Essential Oil. 微胶囊化对柠檬草精油化学成分及抑菌、抗氧化和细胞毒性的影响
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7470
Anely Maciel de Melo, Rafaela Cristina Turola Barbi, Francisco Lucas Chaves Almeida, Weysser Felipe Cândido de Souza, Atacy Maciel de Melo Cavalcante, Hugo Junior Barboza de Souza, Diego Alvarenga Botrel, Soraia Vilela Borges, Roberto Germano Costa, Max Rocha Quirino, Solange de Sousa
{"title":"Effect of Microencapsulation on Chemical Composition and Antimicrobial, Antioxidant and Cytotoxic Properties of Lemongrass (<i>Cymbopogon flexuosus</i>) Essential Oil.","authors":"Anely Maciel de Melo,&nbsp;Rafaela Cristina Turola Barbi,&nbsp;Francisco Lucas Chaves Almeida,&nbsp;Weysser Felipe Cândido de Souza,&nbsp;Atacy Maciel de Melo Cavalcante,&nbsp;Hugo Junior Barboza de Souza,&nbsp;Diego Alvarenga Botrel,&nbsp;Soraia Vilela Borges,&nbsp;Roberto Germano Costa,&nbsp;Max Rocha Quirino,&nbsp;Solange de Sousa","doi":"10.17113/ftb.60.03.22.7470","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7470","url":null,"abstract":"SUMMARY Research background Lemongrass (Cymbopogon flexuosus) essential oil exhibits antimicrobial and antioxidant properties due to the presence of α-citral and β-citral. Essential oils are susceptible to volatilization and oxidation when applied to food matrices. Therefore, a barrier is needed to protect this material. The present study aims to produce microparticles containing lemongrass essential oil, with gum arabic and maltodextrin using spray drying technology. Experimental approach Lemongrass essential oil was extracted by the hydrodistillation method and later microencapsulated with different wall materials. Free and microencapsulated lemongrass essential oil was evaluated for the cytotoxic activity (using Artemia salina as test sample), chemical composition (GC-MS), encapsulation efficiency, antioxidant activity (DPPH, ABTS and FRAP), antimicrobial activity and minimum inhibitory concentration. Results and conclusions The lethal concentration (LC50) of lemongrass essential oil in the cytotoxic test was 8.43 μg/mL against Artemia salina; a high activity that can be associated with the presence of α-citral (~33%) and β-citral (~21%) in the samples, since these were the main compounds with bioactive properties. The highest value of microencapsulation efficiency (88.11%) was obtained when only gum arabic was used as wall material. In general, the microparticles showed satisfactory antioxidant activity (expressed as Trolox equivalents, between 348.66 and 2042.30 µmol/100 g) and bactericidal effect in vitro against Gram-positive and Gram-negative microorganisms. In conclusion, the microencapsulated lemongrass essential oil is a promising functional additive in the food and pharmaceutical industries. Novelty and scientific contribution This study shows that microparticles containing lemongrass essential oil can be prepared using gum arabic and maltodextrin as wall materials by spray drying, resulting in high microencapsulation efficiency. The drying process maintained the antimicrobial and antioxidant properties of the essential oil. Therefore, the microencapsulated lemongrass essential oil is considered a natural, functional and promising additive in the food industry. Its antimicrobial action can increase the shelf life of fresh and semi-fresh products such as cheese, yogurts and meat products. In addition, its antioxidant action can delay the lipid and protein oxidation in food products.","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"386-395"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Fruit Extract Derived from a Mixture of Noni, Pineapple and Mango Capable of Coagulating Milk and Producing Curd with Antidiabetic Activities. 从诺丽、菠萝和芒果混合物中提取的具有凝乳和凝乳抗糖尿病活性的水果提取物。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7456
Jaya Vejayan, Rupbansraaj Bathmanathan, Sharifah Aminah Tuan Said, Srikumar Chakravarthi, Halijah Ibrahim
{"title":"Fruit Extract Derived from a Mixture of Noni, Pineapple and Mango Capable of Coagulating Milk and Producing Curd with Antidiabetic Activities.","authors":"Jaya Vejayan, Rupbansraaj Bathmanathan, Sharifah Aminah Tuan Said, Srikumar Chakravarthi, Halijah Ibrahim","doi":"10.17113/ftb.60.03.22.7456","DOIUrl":"10.17113/ftb.60.03.22.7456","url":null,"abstract":"<p><strong>Research background: </strong><i>Morinda citrifolia</i> L. (noni), <i>Ananas comosus</i> L. cv. Sarawak (pineapple) and <i>Mangifera indica</i> L. cv. Apple (mango) represent fruits capable of coagulating milk and forming a curd. Plant-derived milk coagulants have antidiabetic phytochemicals that enrich the curd. Hence this work evaluates the dual benefits of the fruits in coagulating milk and the antidiabetic activities found in the obtained curd.</p><p><strong>Experimental approach: </strong>The three fruits were mixed to form a supercoagulant (a milk coagulant mixture of the extracts at a ratio of 1:1:1), and the milk coagulation time was measured. The milk was coagulated by the supercoagulant, and thus fortified curd was tested for its ability to inhibit α-glucosidase and α-amylase activities. Then, the fortified curd was fed daily to streptozotocin-induced diabetic rats and their biochemical markers such as blood glucose level, aspartate aminotransferase, alanine transaminase, <i>etc.</i> as well as histopathology of their liver and kidney tissues were compared with the untreated diabetic rats and normal rats.</p><p><strong>Results and conclusion: </strong>The supercoagulant had a milk coagulation time of (28±3) s at a 50 mg/mL concentration. Its fortified curd inhibited α-glucosidase and α-amylase activities, with IC<sub>50</sub> values of (4.04±0.03) and (3.42±0.02) mg/mL, respectively. The average mass of the streptozotocin-induced diabetic rats fed daily with curd formed by the supercoagulant was (201±10) g on day 20 compared to diabetic control rats with (149±16) g. The blood glucose concentration for rats treated with the supercoagulant after fasting was (15±1) mmol/L compared to the diabetic control rats ((26±2) mmol/L). Blood tests on the treated rats showed aspartate aminotransferase, alanine transaminase, γ-glutamyl transferase and alkaline phosphatase (liver function tests) amounts of (214±78), (91±13), 3 and (510±138) U/L, respectively, while the total protein and renal function tests showed the concentrations of albumin, globulin, urea and creatinine of (37±2) g/L, (30±2) g/L, (11±1) mmol/L and (42±3) µmol/L, respectively. These concentrations were found to be similar to those of the normal rats on day 20. Furthermore, a histopathological study performed on the liver and kidney of the rats found no apparent damage.</p><p><strong>Novelty and scientific contribution: </strong>This supercoagulant derived from a mixture of fruits is able to coagulate milk rapidly, and its curd is fortified with safe antidiabetic agents. The supercoagulant is potentially useful in producing functional dairy food to prevent diabetes or as a supplement for diabetics to control their blood sugar. Such products are capable of replacing dairy products derived from animal enzymes and provide consumers with additional functional dairy products.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"375-385"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzymatic Treatment of Spent Green Tea Leaves and Their Use in High-Fibre Cookie Production. 废绿茶的酶处理及其在高纤维饼干生产中的应用。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7474
Ngoc Doan Trang Nguyen, Thi Thuy Huong Phan, Thi Thu Tra Tran, Nu Minh Nguyet Ton, Dinh Le Tam Vo, Van Viet Man Le
{"title":"Enzymatic Treatment of Spent Green Tea Leaves and Their Use in High-Fibre Cookie Production.","authors":"Ngoc Doan Trang Nguyen,&nbsp;Thi Thuy Huong Phan,&nbsp;Thi Thu Tra Tran,&nbsp;Nu Minh Nguyet Ton,&nbsp;Dinh Le Tam Vo,&nbsp;Van Viet Man Le","doi":"10.17113/ftb.60.03.22.7474","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7474","url":null,"abstract":"<p><strong>Research background: </strong>By-products of food industry have been studied as sources of high fibre and antioxidant ingredients for healthy food products, because of their economic and environmental benefits. However, the soluble dietary fibre content of these materials is usually lower than the recommended value that is claimed to bring positive health effects. Enzymatic treatment could be an efficient method for modifying insoluble and soluble dietary fibre contents of these materials. The purpose of this study is to investigate the effects of enzymatic treatment conditions on soluble, insoluble and total dietary fibre mass fractions in spent green tea leaves, and evaluate the quality of dough and cookies when different mass fractions of untreated and treated leaves were added to the recipe.</p><p><strong>Experimental approach: </strong>The mass fractions of soluble, insoluble and total dietary fibre in spent tea leaf powder was evaluated after the leaves were treated with cellulase amount of 0-25 U/g for 0 to 2 h. Wheat flour was replaced by untreated and treated spent tea leaf powder at 0, 10, 20, 30 and 40% in cookie formulation. Textural properties of dough, proximate composition, physical properties and overall acceptability of cookies were analysed.</p><p><strong>Results and conclusions: </strong>The appropriate conditions for enzymatic treatment were enzyme loading of 20 U/g and biocatalytic time of 1.5 h, under which the mass fraction of soluble dietary fibre in spent tea leaves increased by 144.5% compared to that of the control sample. The addition of spent tea leaves led to the increase in dough hardness. Increase in the spent tea leaf amount also enhanced fibre mass fraction, antioxidant activity and hardness of cookies but reduced their overall acceptability. Moreover, the enzymatic treatment of spent tea leaves improved the soluble to total dietary fibre ratio of the cookies, which influenced their textural properties and health benefits. The cookies with added 20% untreated or treated spent tea leaves were overall accepted by the panel.</p><p><strong>Novelty and scientific contribution: </strong>For the first time, spent tea leaves have been treated with enzymes to improve their soluble to total dietary fibre ratio. The treated spent tea leaves are a new promising high-fibre antioxidant ingredient for cookie preparation.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"396-405"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Free Radical Scavenging, Redox Balance and Wound Healing Activity of Bioactive Peptides Derived from Proteinase K-Assisted Hydrolysis of Hypophthalmichthys molitrix Skin Collagen. 蛋白酶k辅助水解小眼鱼皮肤胶原蛋白生物活性肽的自由基清除、氧化还原平衡和伤口愈合活性
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7107
Daniela Ilie, Andreea Iosageanu, Oana Craciunescu, Ana-Maria Seciu-Grama, Catalina Sanda, Florin Oancea
{"title":"Free Radical Scavenging, Redox Balance and Wound Healing Activity of Bioactive Peptides Derived from Proteinase K-Assisted Hydrolysis of <i>Hypophthalmichthys molitrix</i> Skin Collagen.","authors":"Daniela Ilie,&nbsp;Andreea Iosageanu,&nbsp;Oana Craciunescu,&nbsp;Ana-Maria Seciu-Grama,&nbsp;Catalina Sanda,&nbsp;Florin Oancea","doi":"10.17113/ftb.60.03.22.7107","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7107","url":null,"abstract":"<p><strong>Research background: </strong>Various protocols for enzymatic hydrolysis of fish by-products are increasingly tested to ensure value-added products with functional and biological properties important for food, cosmetic and medical applications. In addition, they attempt to minimize waste from industrial processing and environmental requirements. This study aims to establish an efficient protocol based on two-step enzymatic hydrolysis of freshwater fish skin and to evaluate the effect of resulting bioactive peptides on free radical scavenging, redox balance and regulation of fibroblast proliferation and migration.</p><p><strong>Experimental approach: </strong>Pepsin-soluble collagen extracted from silver carp (<i>Hypophthalmichthys molitrix</i>) skin was hydrolyzed by proteinase K at specific sites under controlled conditions. The molecular mass of ultrafiltration permeate was determined by gradient electrophoresis and gel filtration chromatography. The biological activity of intermediate and small size bioactive peptides was evaluated in experimental models <i>in vitro</i> mimicking oxidative stress and skin wound conditions.</p><p><strong>Results and conclusions: </strong>Extracted fish collagen was hydrolysed using proteinase K, the most efficient enzyme for the cleavage of the primary structure of the molecule, as previously found <i>in silico</i>. Established optimal conditions increased the enzyme specificity and the process yield. Bioactive peptides exerted significantly higher scavenging activity on free stable radicals and hydroxyl radicals often found <i>in vivo</i>, compared to fish collagen. They stimulated fibroblast metabolism in a dose-dependent manner and up-regulated cell migration in a scratch wound model. Pretreatment of fibroblasts with induced oxidative stress using optimal concentrations of fish peptides prevented the increase of reactive oxygen species production. In conclusion, bioactive peptides from carp skin demonstrated valuable properties of maintaining redox balance and skin wound healing process improvement, which indicated further potential applications in the development of pharmaceutical and nutraceutical formulations.</p><p><strong>Novelty and scientific contribution: </strong>In this study the enzymatic hydrolysis was applied to isolated protein, in contrast to previous studies using waste tissue with variable composition. Recovered bioactive peptides acted not only as antioxidant agents, but also as regulators of oxidative stress and wound healing processes in skin cell models. Their nutritional and cosmetic application is recommended in novel formulations fighting skin ageing phenomena.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"281-292"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Novel Vigna subterranea (L.) Verdc Soluble Dietary Fibre-Starch Nanocomposite: Functional and Antioxidant Characteristics. 新型 Vigna subterranea (L.) Verdc 可溶性膳食纤维-淀粉纳米复合材料:功能和抗氧化特性。
IF 2.3 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7409
Yvonne Maphosa, Victoria Adaora Jideani, Daniel Imwansi Ikhu-Omoregbe
{"title":"Novel <i>Vigna subterranea</i> (L.) Verdc Soluble Dietary Fibre<i>-</i>Starch Nanocomposite: Functional and Antioxidant Characteristics.","authors":"Yvonne Maphosa, Victoria Adaora Jideani, Daniel Imwansi Ikhu-Omoregbe","doi":"10.17113/ftb.60.03.22.7409","DOIUrl":"10.17113/ftb.60.03.22.7409","url":null,"abstract":"<p><strong>Research background: </strong>Bambara groundnut (<i>Vigna subterranea</i> (L.) Verdc.) is a great source of soluble dietary fibre and starch. Bambara groundnut soluble dietary fibre is rich in bioactive compounds, namely, uronic acids (11.8%) and hydrolysable polyphenols (expressed as gallic acid equivalents (GAE) 20 mg/g), with crucial physiological and functional benefits. The industrial use of native starch is limited because of the inherent undesirable attributes that render it unstable. The aim of this study is to characterise the antioxidant, functional and physicochemical properties of Bambara groundnut starch-soluble dietary fibre nanocomposite (hereafter groundnut nanocomposite).</p><p><strong>Experimental approach: </strong>The pasting properties by rapid visco analysis, chemical composition, hydration properties, oil-binding capacity, emulsifying activity index (EAI), emulsion stability index (ESI) and antioxidant properties of Bambara groundnut starch, soluble fibre and nanocomposite were studied.</p><p><strong>Results and conclusions: </strong>The Bambara groundnut soluble dietary fibre and nanocomposite did not exhibit typical pasting properties. The nanocomposite had high mass fraction of carbohydrates (78.7%) and proteins (7.0%), low mass fraction of fat (0.8%) and had a considerable mass fraction of ash (4.9%). The Bambara groundnut starch, soluble dietary fibre and nanocomposite showed significant (p<0.001) differences in solubility. Their EAI values were 23.2, 85.7 and 90.6%, respectively, and the ESI values were 23.3, 87.1 and 87.5%, respectively. The three biopolymers differed significantly (p<0.001) in all colour characteristics: lightness (<i>L</i>*), redness/greenness (<i>a</i>*), yellowness/blueness (<i>b</i>*), chroma and hue angles. Their polyphenolic mass fraction, expressed as GAE, was 0.10, 6.6 and 0.46 mg/g, respectively, and their ferric reducing antioxidant power values, expressed as ascorbic acid equivalents (AAE), were 1.2, 4.8 and 1.4 μmol/g, respectively. The phenolic compounds (in mg/g): chlorogenic acid 18, monocrotaline 20, luteolin 7-O-(6''-malonylglucoside) 4 and casuarine 6-α-d-glucoside 27 were present in the dietary fibre but absent from the starch and nanocomposite. The Bambara groundnut nanocomposite possesses desirable physicochemical and antioxidant properties, making it suitable as an ingredient in various food systems.</p><p><strong>Novelty and scientific contribution: </strong>Nanocomposites have the potential to revolutionise the food industry but their study as food ingredients is very limited. Furthermore, nothing is known about the physicochemical, functional and antioxidant characteristics of Bambara groundnut nanocomposite, thus investigating these properties will address this knowledge gap.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"361-374"},"PeriodicalIF":2.3,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality Factors of Commercial Snail Fillets as Affected by Species. 商品螺片品质因子受品种影响。
IF 2.4 4区 农林科学
Food Technology and Biotechnology Pub Date : 2022-09-01 DOI: 10.17113/ftb.60.03.22.7403
Efkarpia Kougiagka, Chrysoula Apostologamvrou, Persephoni Giannouli, Marianthi Hatziioannou
{"title":"Quality Factors of Commercial Snail Fillets as Affected by Species.","authors":"Efkarpia Kougiagka,&nbsp;Chrysoula Apostologamvrou,&nbsp;Persephoni Giannouli,&nbsp;Marianthi Hatziioannou","doi":"10.17113/ftb.60.03.22.7403","DOIUrl":"https://doi.org/10.17113/ftb.60.03.22.7403","url":null,"abstract":"<p><strong>Research background: </strong>This study fulfils a need for investigation of a quality profile of snail fillets. Edible snails are a famous food product consumed worldwide and treated as delicacy. Nutritional value, colour and textural properties, such as hardness, are critical factors that impact consumer acceptance of the product. Hardness of snail meat is affected by its native original microstructure.</p><p><strong>Experimental approach: </strong>Fresh snails of the farmed species <i>Cornu aspersum maximum,</i> wild and farmed <i>Cornu aspersum aspersum</i> and wild <i>Helix lucorum</i> were used in order to investigate the qualitative profile of snail meat. Proximate composition, hardness and colour measurements were conducted on fillets of all species. The histological structure of the fillets of <i>Cornu aspersum maximum</i> was examined.</p><p><strong>Results and conclusions: </strong>Quality parameters of snail fillets were studied. A novel method of hardness analysis was proposed where the cylindrical part of snail fillets from the mid-posterior region with specific geometry 6 mm diameter and 6 mm height was used. The suitability of the mid-posterior region was enhanced by the uniform structure confirmed by the histological analysis. <i>Helix lucorum</i> snail fillet had the highest energy content and the highest hardness but the lowest carbohydrate content. The species <i>Cornu aspersum maximum</i> was evaluated with the highest values of <i>a*</i> (redness), <i>b*</i> (yellowness) and <i>C</i>* (chroma) compared to other species. Parameter <i>L*</i> (lightness) of wild snail fillets was lower than of the farmed ones due to age, diet, farming or environmental conditions, but it could also be related to snail carbohydrate content.</p><p><strong>Novelty and scientific contribution: </strong>This study yielded notable results on qualitative characteristics of snail fillets as food and important information is given on its meat properties. Furthermore, a novel methodology of hardness is provided in order to minimize natural, breeding and environmental influences. Finally, the research outcomes could lead to proper handling methods for further fabrication of snail meat.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":"60 3","pages":"330-337"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40448736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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