栽培和野生石榴可食用和不可食用部位的多酚成分、抗氧化和抗增殖活性

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mirjana Milošević, Jelena Vulić, Zoran Kukrić, Biljana Lazić, Dragana Četojević-Simin, Jasna Čanadanović-Brunet
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引用次数: 0

摘要

研究背景。本研究的目的是测定和比较栽培和野生石榴果实的果皮、假皮和膜的汁液和提取物的抗氧化和抗增殖活性。实验方法。分光光度法测定了总酚、总黄酮、总黄酮醇、总黄酮-3-醇和总花青素的含量。用高效液相色谱法测定各酚类物质含量。采用DPPH和ABTS法检测其抗氧化活性和羟自由基中和活性,采用硫代丹胺B (SRB)法检测其体外抗增殖活性。结果和结论。总酚含量以野生石榴皮提取物(WPPE)最高,为340.92 mg QE/g (p<0.05),总黄酮含量以栽培石榴皮提取物(CPPE)最高,为31.84 mg QE/g (p<0.05)。WPPE样品对游离DPPH和ABTS自由基的抗氧化活性最高。CPPE和CPME(分别为石榴皮和石榴膜提取物)对羟基自由基的抑制作用几乎相同,且效果最强(分别为41.24 μg/mL和41.23 μg/mL)。与所有其他样品相比,WPPE样品对所有测试的肿瘤细胞系的生长抑制作用最强。创新和科学贡献。本研究对栽培和野生石榴不同部位的生物活性进行了测定和比较。在现有文献中,仅对石榴果实部分部位的抗氧化和抗增殖活性进行了研究。在这项工作中,研究了石榴果实的所有部分,包括石榴的膜,这在其他作品中很少分析。此外,野生石榴在以前的研究中较少被调查。未来的研究应集中在获得的石榴样品的体内研究上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polyphenolic Composition, Antioxidant and Antiproliferative Activity of Edible and Inedible Parts of Cultivated and Wild Growing Pomegranate (Punica granatum L.)
Research background. The aim of this study was to determine and compare the antioxidant and antiproliferative activities of juices and extracts of the peel, aril and membrane of the cultivated and wild growing pomegranate fruits. Experimental approach. The contents of total phenols, total flavonoids, total flavonols, total flavan-3-ols, and total anthocyanins were determined spectrophotometrically. Individual phenolics were quantified by HPLC. Antioxidant activity was detected by DPPH and ABTS test, and neutralization of hydroxyl radical, while in vitro antiproliferative activity by sulforhodamine B (SRB) assay. Results and conclusions. Total phenols were statistically the highest in wild pomegranate peel extract (WPPE) and amounted 340.92 mg GAE/g (p<0.05), while the content of total flavonoids was the highest in cultivated pomegranate peel extract (CPPE) and amounted 31.84 mg QE/g (p<0.05). The WPPE sample showed the highest antioxidant activity with respect to free DPPH and ABTS radicals. The CPPE and CPME (cultivated pomegranate peel and membrane extracts, respectively) samples showed almost identical and the strongest effect on hydroxyl-radical inhibition (41.24 μg/mL and 41.23 μg/mL, respectively). The WPPE sample showed the strongest effect on the growth inhibition of all tested tumor cell lines compared to all other samples. Novelty and scientific contribution. In this study, bioactivity of different parts of cultivated and wild pomegranates were determined and compared. In the available literature, the individual antioxidant and antiproliferative activity of only some parts of pomegranate fruit were studied. In this work, all parts of the pomegranate fruit were investigated, including the membrane of the pomegranate, which was barely analyzed in other works. Also, wild pomegranate is less investigated in previous studies. Future research should be focused on in vivo studies of obtained pomegranate samples.
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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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