Folia neuropathologica最新文献

{"title":"Cytidine does not affect acute toxicity of intravenously administered choline.","authors":"Kamil Synoradzki, Maciej Swiatkiewicz, Paweł Grieb","doi":"10.5114/fn.2024.140501","DOIUrl":"https://doi.org/10.5114/fn.2024.140501","url":null,"abstract":"<p><p>Cytidine-5'-diphosphocholine (CDP-choline) is a key precursor for the intracellular synthesis of phosphatidylcholine and other phospholipids. Following either intravenous or oral application citicoline (CDP-choline of exogenous origin) undergoes quick decomposition to cytidine and choline, and for this reason it is frequently considered a prodrug. However, upon acute intravenous application in mice citicoline is, on a molar basis, 20 times less toxic than choline. To find out whether cytidine may attenuate toxicity of choline, in the present experiments we compared maximum tolerated doses of single intravenous injections of choline and equimolar mixture of choline and cytidine. We assumed that, if after oral intake a substantial part of citicoline is catabolised already in the intestine and its catabolites enter blood separately, intravenously applied equimolar mixture of cytidine and choline will be markedly less toxic than an equivalent molar dose of choline. However, the maximum tolerated single doses determined in our experiment for choline and equimolar mixture of choline and cytidine were similar. These data suggest that citicoline taken orally is not significantly decomposed in the intestinal lumen, but absorbed to blood as the intact molecule.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 2","pages":"120-126"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitor of bromodomain and extraterminal domain proteins decreases transcription of Cd33 in the brain of mice subjected to systemic inflammation; a promising strategy for neuroprotection.","authors":"Grzegorz A Czapski, Marta Matuszewska, Magdalena Cieślik, Joanna B Strosznajder","doi":"10.5114/fn.2024.138140","DOIUrl":"10.5114/fn.2024.138140","url":null,"abstract":"<p><p>The neuroinflammation is a crucial component of virtually all neurodegenerative disorders, including Alzheimer's disease (AD). The bacterial lipopolysaccharide (LPS), a potent activator of the innate immune system, was suggested to influence or even trigger the neuropathological alterations in AD. LPS-induced neuroinflammation involves changes in transcription of several genes, thus controlling these molecular processes may be a potentially efficient strategy to attenuate the progression of AD. Since genome-wide association studies showed that the majority of AD-related genetic risk factors (AD-GRF) are connected to the immune system, our aim was to identify AD-GRF affected in the hippocampus by LPS-induced systemic inflammatory response (SIR). Moreover, we analysed the role of bromodomain and extraterminal domain (BET) proteins, the readers of the acetylation code, in controlling the transcription of selected AD-GRF in the brain during neuroinflammation. In our study, we used a mouse model of LPS-induced SIR and mouse microglial BV2 cells. JQ1 was used as an inhibitor of BET proteins. The level of mRNA was analysed using microarrays and qPCR. Our data demonstrated that among the established AD-GRF, only the expression of Cd33 was significantly upregulated in the hippocampus during SIR. In parallel, we observed an increase in the expression of Brd4, a BET family member. JQ1 prevented an LPS-evoked increase in Cd33 expression in the hippocampus of mice. Moreover, JQ1 reduced Cd33 expression in BV2 microglial cells stimulated with blood serum from LPS-treated mice. Our study suggests that LPS-evoked SIR may increase Cd33 gene expression in the brain, and inhibition of BET proteins through suppression of Cd33 expression could be a promising strategy in prevention or in slowing down the progression of neuroinflammation and may potentially affect the pathomechanism of AD.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 2","pages":"127-135"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of serum miR-17-92 cluster in ischemic stroke.","authors":"Lihua Dong, Yuanshen Ye, Guiyuan Huang, Hongmiao Tao","doi":"10.5114/fn.2024.138680","DOIUrl":"10.5114/fn.2024.138680","url":null,"abstract":"<p><strong>Introduction: </strong>Ischemic stroke (IS) is a prevalent disease that poses a significant threat to human life and is responsible for a substantial financial burden. Research has established the crucial role of the miR-17-92 cluster in lung cancer, cardiovascular diseases, and traumatic brain injury. Despite this, few research studies had fully detected the potential of the miR-17-92 cluster as a novel circulating marker for diagnosing IS.</p><p><strong>Material and methods: </strong>miR-17-92 cluster expression in IS was investigated using GSE117064 dataset via bioinformatics analysis. Moreover, qRT-PCR was conducted to further verify miR-17-92 cluster expression in 58 IS individuals and 50 healthy controls (HCs). These cluster members were examined regarding their potential for detecting and diagnosing IS using the ROC method.</p><p><strong>Results: </strong>The expression level of serum miR-20a-5p, miR-19a-3p, miR-18a-5p, and miR-19b-3p was considerably lowered in IS in contrast with HC in both the GSE117064 cohort and clinical cohort. Moreover, these four miRNAs had a fair performance in IS detection. Thereafter, a diagnostic model based on these aforementioned four miRNAs was developed by logistic regression, which had an AUC of 0.974 in the ROC curve. This diagnostic module was verified using the GSE117064 dataset. Further analysis demonstrated an increasing level of the aforementioned miRNAs in day-7 IS patients compared with day-1 IS patients.</p><p><strong>Conclusions: </strong>This research verified the downregulation of the miR-17-92 cluster in IS. This diagnostic model enrolling four cluster members may be a promising biomarker for IS detection.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 2","pages":"206-214"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An enriched environment promotes cognitive recovery and cerebral blood flow in aged mice under sevoflurane anaesthesia.","authors":"Wenfeng Gao, Wenji Xie, Wenqin Xie, Changcheng Jiang, Zhenming Kang, Naizhen Liu","doi":"10.5114/fn.2024.136017","DOIUrl":"10.5114/fn.2024.136017","url":null,"abstract":"<p><p>Sevoflurane is an inhalation anaesthetic agent widely used in clinical settings. Despite good surgical outcomes using sevoflurane, patients frequently develop postoperative cognitive dysfunction (POCD). An enriched environment (EE), as a rehabilitation technique, could provide objects and tools to facilitate neuromotor and visual stimuli and brain activity, and is reported to improve cognitive functions. We aim to investigate the impairments of sevoflurane inhalation on cognitive function in mice and determine the benefits of EE in ameliorating POCD. Eighteen-month-old mice were exposed to sevoflurane inhalation for 2 h and then placed in standard environment (SE) or EE cages. The mice without sevoflurane exposure in standard or EE cages were used as controls. The behavioural tests include Morris water maze, Y maze and novel object recognition. Magnetic resonance imaging (MRI) was used to determine the blood circulation in the brains. The proangiogenic factors (CD31, angiopoietin-1, vascular endothelial growth factor, and N-cadherin) and neurotrophic (brain-derived neurotrophic factor, post-synaptic density protein 95) expression in hippocampus of aged mice were evaluated by Western blotting and RT-PCR analysis. Sevoflurane-exposed mice demonstrated reduced performance in learning, memory and spatial memory tests. Enriched environment improved the behavioural performance of sevoflurane-exposed animals. Sevoflurane exposure reduced the blood flow in the brains, and these effects were ameliorated by EE habitation. The EE also promoted the expression of angiogenic and neurotropic factors in sevoflurane-exposed animals. In summary, EE is effective in ameliorating the side-effects of sevoflurane exposure in aged mice.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":" ","pages":"312-320"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZDHHC16 promoted neurocyte ferroptosis by suppression of CREB in a cerebral apoplexy model.","authors":"Dongmei Xu, Hua Liu, Xiaoyu Deng, Jifan Fu","doi":"10.5114/fn.2024.145878","DOIUrl":"https://doi.org/10.5114/fn.2024.145878","url":null,"abstract":"<p><strong>Introduction: </strong>The present study explored the effects and possible mechanisms of ZDHHC16 in a model of cerebral apoplexy (CA).</p><p><strong>Material and methods: </strong>Patients with CA were collected from our hospital. Mice were used to establish an middle cerebral artery occlusion (MCAO) model.</p><p><strong>Results: </strong>ZDHHC16 levels in patients with CA were up-regulated. ZDHHC16 up-regulation promoted inflammation and accelerated mitochondrial damage in the in vitro model. ZDHHC16 gene up-regulation promoted ferroptosis of neurocytes. The inhibition of ZDHHC16 prevented cerebral apoplexy in the mouse model. ZDHHC16 up-regulation suppressed CREB through interlinkage of CREB by promoting CREB ubiquitination. CREB agonists inhibited the effects of ZDHHC16 up-regulation in the in vitro model. CREB inhibitor inhibited the effects of ZDHHC16 down-regulation in the in vitro model.</p><p><strong>Conclusions: </strong>We conclude that ZDHHC16 promoted ferroptosis and inflammation in a model of CA through the suppression of CREB. The findings might be of benefit in the treatment of CA or other nervous system diseases.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 4","pages":"386-395"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report of glioma with cerebral hemorrhage as the first symptom.","authors":"Tao Xie, Xue-Xin He, Qian Sun, Zhuo-Feng Mao, Xiao-Peng Wang","doi":"10.5114/fn.2024.145177","DOIUrl":"https://doi.org/10.5114/fn.2024.145177","url":null,"abstract":"<p><strong>Introduction: </strong>High-grade gliomas are the most common and most lethal primary cancers of the central nervous system. Glioma patients with initial symptoms of cerebral hemorrhage are prone to clinical misdiagnosis and delayed diagnosis.</p><p><strong>Case summary: </strong>This paper reports the clinical data of a 40-year-old man with glioma who initially presented with cerebral hemorrhage. Cerebral computed tomography (CT) revealed left parietal cerebral hemorrhage, while contrast-enhanced magnetic resonance imaging (MRI) revealed abnormal enhancement of the left frontoparietal junction, indicating internal bleeding of metastatic tumor. Pathological examination confirmed a high-grade glioma, with immunohistochemistry indicating positive glial fibrillary acidic protein (GFAP) and 40% Ki-67 positive labeling. Consequently, the patient received a final diagnosis of glioma (WHO grade IV).</p><p><strong>Conclusions: </strong>We report an interesting case in which glioma initially presented with cerebral hemorrhage. Therefore, gliomas should be considered as a possible cause of cerebral hemorrhage in patients without risk factors for hemorrhage.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 4","pages":"456-459"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of gliomas based on genome-wide DNA methylation profiles and immunohistochemical analysis: a short study.","authors":"Olga Taryma-Leśniak, Kamil Buczkowski, Tomasz Klepinowski, Maciej Jankowski, Szymon Retfiński, Bartłomiej Sagan, Leszek Sagan, Ewa Szutowicz, Kacper Winiarski, Marta Masztalewicz, Przemysław Nowacki, Marta Sobalska-Kwapis, Dominik Strapagiel, Wojciech Kloc, Beata Lipska-Ziętkiewicz, Ewa Iżycka-Świeszewska, Tomasz K Wojdacz","doi":"10.5114/fn.2024.147538","DOIUrl":"https://doi.org/10.5114/fn.2024.147538","url":null,"abstract":"<p><p>Methylation profiling is one of the diagnostic methods included in the 2021 World Health Organization Classification of Central Nervous System Tumors (WHO CNS5). In the present short study, we performed methylation analysis of 16 archival diffuse gliomas, including seven glioblastomas (GB) with long survival and three GB with early disease presentation. The formalin-fixed paraffin-embedded (FFPE) and freshly frozen (FF) samples were analyzed using Infinium Methylation-EPIC microarrays. The diagnosis was changed in two cases, and two other cases were classified as control tissue due to the low content of neoplastic cells in examined frozen samples. No differences were found between the usefulness of FF and FFPE material for methylation profiling. Copy number variation analysis and comparative immunohistochemical and molecular studies were also performed. The MGMT promoter methylation assessment was consistent between the micro-array and methylation-sensitive high-resolution melting (MS-HRM) testing. The performed analyses did not supply new data according to the unexpected clinical course of the selected cases. Immunohistochemical evaluation with p16 and PTEN antibodies was consistent with the CDKN2A and PTEN status in most cases. In conclusion, the classification of gliomas based on genome-wide methylation profiles increases the precision of histopathological diagnosis but has limitations, and pathological and clinical consistency is always necessary. Furthermore, immunohistochemical surrogates of molecular alterations are suitable and widely available diagnostic tools.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 4","pages":"362-375"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimizing hemorrhagic and perioperative complications in deep brain stimulation surgery in a series of 191 patients operated on over 4 years.","authors":"Michał Sobstyl, Angelika Stapińska-Syniec, Wiktor Paskal","doi":"10.5114/fn.2024.143649","DOIUrl":"https://doi.org/10.5114/fn.2024.143649","url":null,"abstract":"<p><strong>Introduction: </strong>Intracranial hemorrhage (ICH) in functional neurosurgery is a relatively rare but serious complication. One of the possible risk factors related to ICH is the number of trajectories made for microelectrode recording (MER). Authors who solely rely on macrostimulation using macroelectrodes argue that the incidence of ICH is much lower while maintaining good clinical efficacy of deep brain stimulation (DBS). The present study aimed to assess the incidence of ICH in DBS procedures by reducing to the minimum the number of brain passes and the diameter of guiding cannulas. For this reason, we used one MER guiding cannula exclusively for track making with subsequent macrostimulation done through the implanted DBS electrode.</p><p><strong>Material and methods: </strong>All DBS procedures performed between January 2018 and January 2022 in the Department of Neurosurgery of the Institute of Psychiatry and Neurology in Warsaw were analyzed for possible ICH and other perioperative complications. The DBS lead was implanted by an MR image-guided and intraprocedural CT-verified approach. No MER was done.</p><p><strong>Results: </strong>During four years 191 patients underwent 267 DBS lead implantations in 252 stereotactic procedures. ICH occurred in 2 patients. Both were symptomatic. Adverse symptoms resolved within a week. Two DBS leads required replacement. There was 1 case of hematoma at the implantable pulse generator (IPG) site and 1 case of pneumothorax due to tunneling of the extension.</p><p><strong>Conclusions: </strong>Our surgical technique has a low incidence of ICH. Symptomatic ICH affected 2 patients. The other perioperative complications mentioned above required repeated surgery or conservative treatment. No patient suffered from permanent deficits.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 3","pages":"249-258"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rb1 attenuates mouse cerebral ischemia/reperfusion induced neurological impairments through modulation of microglial polarization.","authors":"Cong Yu, Yisong Zhang, Yijun Guo, Zhiyang Shen, Keqin Li, Wei Chen, Dabin Ren","doi":"10.5114/fn.2024.134300","DOIUrl":"https://doi.org/10.5114/fn.2024.134300","url":null,"abstract":"<p><p>Cerebral ischemia/reperfusion causes high disability, recurrence, and mortality. Ischemic stroke is a powerful stimulus that triggers significant microglia activation. Ginsenoside Rb1 (GS-Rb1) has been demonstrated to have neuroprotective effects in the central nervous system. In this study, the effects of GS-Rb1 against cerebral ischemia/reperfusion were explored. A mouse model of middle cerebral artery occlusion (MCAO) was used to mimic the cerebral ischemia/reperfusion. Mice in MCAO + GS-Rb1 groups received 5, 10, or 20 mg/kg GS-Rb1 through intraperitoneal injection. Modified neurological severity scoring (mNSS) showed neurological function, while the open field test tested the anxiety-like behaviors. Cognitive impairment was evaluated by Morris water maze. Protein levels were evaluated by ELISA and Western blot and mRNA levels were analyzed by qRT-PCR. When compared to the MCAO mice, mice in the MCAO + GS-Rb1 group had significantly lower mNSS scores and less brain water content. GS-Rb1 alleviated both cognitive impairment and anxiety and inhibited microglial activation in the cerebral ischemia/reperfusion model. GS-Rb1 enhanced M2-type microglia polarization while inhibiting M1-type microglia polarization. In summary, we observed that GS-Rb1 had neuro-protective effects in a cerebral ischemia/reperfusion mouse model through regulating the microglia polarization.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 2","pages":"215-222"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis and systematic review of the association between cortisol and the beginning of depression symptoms in adolescents and young adults.","authors":"Wenling Li, Zheng Huang, Ruqi Zhang, Lan Chi, Mengling Wang, Yun Zhang, Jianying Li","doi":"10.5114/fn.2024.142693","DOIUrl":"https://doi.org/10.5114/fn.2024.142693","url":null,"abstract":"<p><strong>Introduction: </strong>The incidence of major depressive disorder (MDD) in adults has been associated with dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis, leading to elevated secretion of cortisol. However, limited research has been done globally on how the dysregulated HPA axis and changed cortisol levels relate to depression in adolescents and young adults. The objective of this research was to conduct a systematic review and meta-analysis of the association between cortisol level, a marker of HPA axis activity, and depression in adolescents and young adults.</p><p><strong>Material and methods: </strong>A systematic search was conducted using four electronic databases (PubMed, EMBASE, Scopus, and Cochrane Library) to select publications published in peer-reviewed journals written in English. The odds ratio (OR) and standard mean difference (SMD) were calculated, along with their 95% confidence intervals. We assessed heterogeneity using Cochrane c2 and I2 statistics and the appropriate P-value. The analysis used RevMan 5.3.</p><p><strong>Results: </strong>The current meta-analysis included 10 cross-sectional studies with a total of 1301 adolescents and young adults across various age cohorts, with 629 depressed and 672 non-depressed individuals. It was found that the likelihood of depression is higher among the included adolescents and young adults, with an OR of 1.62 (95% CI: 0.44-5.92), and depressed individuals have significantly higher cortisol levels (SMD of 0.87 (95% CI 0.43-1.31) and cortisol stress response SMD of 0.68 (95% CI 0.31-1.05)). Furthermore, there was a strong positive linear association ( r = 0.82) between morning and afternoon cortisol levels in adolescents and young adults and depression scores.</p><p><strong>Conclusions: </strong>The results of our study indicate that an increase in both morning and afternoon cortisol levels is associated with the development of depression in adolescence and young adults. Research has indicated that an increased level of cortisol is considered a risk factor for depression during adolescence and a linear correlation exists between cortisol levels and depression scores.</p>","PeriodicalId":12370,"journal":{"name":"Folia neuropathologica","volume":"62 4","pages":"335-347"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}