{"title":"Detection of Etomidate and Etomidate Acid in Urine Using HPLC-MS/MS Method.","authors":"Tian-Fu He, Huan-Hui Zhu, Yuan-Yuan Tian, Yin-Shuang Jin, Xian-Wen Lin, Song-Cai Wang","doi":"10.12116/j.issn.1004-5619.2023.330702","DOIUrl":"https://doi.org/10.12116/j.issn.1004-5619.2023.330702","url":null,"abstract":"<p><strong>Objectives: </strong>To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the detection of etomidate and etomidate acid in urine samples.</p><p><strong>Methods: </strong>Protein in the urine samples was precipitated by adding acetonitrile, and the supernatant was obtained after centrifugation and filtered. The supernatant was separated on a C<sub>18</sub> column with a mobile phase consisting of 0.1% formic acid solution and acetonitrile at a flow rate of 0.4 mL/min. The detection was performed in positive electrospray ionization (ESI) and multiple reaction monitoring (MRM) modes. The method was validated for selectivity, linearity and limit of detection (LOD), and applied to a case of etomidate poisoning death.</p><p><strong>Results: </strong>The LOD of etomidate and etomidate acid were 0.2 and 0.5 ng/mL, respectively, and the limit of quantitation (LOQ) were 0.5 and 1.0 ng/mL, respectively. Good linear relationship was observed within the linear range (<i>r</i>>0.995 0). At three concentration levels (0.5, 5, 50 ng/mL for etomidate and 1, 10, 100 ng/mL for etomidate acid), the matrix effect was within the range of 5.42% to 18.47%, the extraction recovery rate was greater than 84.25% and the stability was greater than 88.23%. The accuracy, precision and dilution reliability all met the experimental requirements. Etomidate and etomidate acid were successfully detected with the concentrations of 8.82 and 27.88 μg/mL in the urine of a deceased individual who had consumed excessive etomidate.</p><p><strong>Conclusions: </strong>The method has simple pretreatment, high sensitivity and wide linear range, which can be applied to the detection of etomidate and etomidate acid in urine samples in forensic science.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"40 5","pages":"454-460"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument.","authors":"Jun-Ping Han, Ying-Xi Wang, Chao Liu, Feng Liu, Jin-Rong Guo, Wei Zhu, Guang-Feng Tang, Cai-Xia Li, Xin Wang, Zhang-Ping Jiao","doi":"10.12116/j.issn.1004-5619.2023.530601","DOIUrl":"https://doi.org/10.12116/j.issn.1004-5619.2023.530601","url":null,"abstract":"<p><strong>Objectives: </strong>To verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument (hereinafter referred to as Quick TargSeq DNA detection instrument) and evaluate its forensic application prospects.</p><p><strong>Methods: </strong>A total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security (4 individuals, 20 samples) and five participating institutions (54 samples) were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges. The results were compared with the known genotypes to calculate the samples' detection rate and genotyping accuracy.</p><p><strong>Results: </strong>The results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes. The detection rate of the 74 samples was 100%, and the genotyping accuracy was 97.33%.</p><p><strong>Conclusions: </strong>The Quick TargSeq DNA detection instrument has good stability and high genotyping accuracy.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"40 5","pages":"461-467"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
法医学杂志Pub Date : 2024-10-25DOI: 10.12116/j.issn.1004-5619.2023.430306
法医学杂志Pub Date : 2024-10-25DOI: 10.12116/j.issn.1004-5619.2023.431208
Qi-Rui Han, Wen-Ji Zhang, Hao-Yang Li, Ying-Chao Luo
{"title":"Current Status and Prospects of Bloodstain Age Estimation Technology.","authors":"Qi-Rui Han, Wen-Ji Zhang, Hao-Yang Li, Ying-Chao Luo","doi":"10.12116/j.issn.1004-5619.2023.431208","DOIUrl":"https://doi.org/10.12116/j.issn.1004-5619.2023.431208","url":null,"abstract":"<p><p>Bloodstains are important biological evidence at violent crime scenes and contain rich information about the crime. The relatively accurate estimation of bloodstain age is of great significance for estimating the time of the crime, confirming or excluding the association of relevant persons (objects) with the case. In recent years, researchers at home and abroad have conducted extensive studies on the bloodstain age estimation from the aspects of bloodstain image and biochemical characteristics. This paper reviews the methods of bloodstain age estimation in the past two decades, discusses the obstacles of these methods in investigative practice, and provides an outlook on the development of bloodstain age estimation technology.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"40 5","pages":"468-475"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
法医学杂志Pub Date : 2024-10-25DOI: 10.12116/j.issn.1004-5619.2024.440306
法医学杂志Pub Date : 2024-08-25DOI: 10.12116/j.issn.1004-5619.2023.531208
Ming-Hui Jin, Ya Li, Shao-Hua Yi
{"title":"Research Progress on the Application of Human Body Surface Microbiome to Forensic Individual Identification.","authors":"Ming-Hui Jin, Ya Li, Shao-Hua Yi","doi":"10.12116/j.issn.1004-5619.2023.531208","DOIUrl":"https://doi.org/10.12116/j.issn.1004-5619.2023.531208","url":null,"abstract":"<p><p>Skin and hair are the outermost interfaces between the human body and the external environment with many microorganisms distributed. These body surface microorganisms have individual specificity and spatial temporal stability and can be transferred to the surface of other objects through contact, and can be easily found at the crime scene, which helps to construct the connection between the suspect and the crime scene. When the sample is degraded or the DNA quantity is too low to obtain sufficient human DNA evidence, the highly amplified copies of body surface microbiome analysis can be used as an adjunct to human DNA typing without affecting DNA extraction and typing, and provide useful information for narrowing the range of suspects. This paper reviews the forensic characteristics and analytical methods of body surface microbiome, the research progress on the application of body surface microbiome to forensic individual identification, and puts forward the challenges of applying body surface microbiome to forensic practice.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"40 4","pages":"379-386"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
法医学杂志Pub Date : 2024-08-25DOI: 10.12116/j.issn.1004-5619.2024.440303
Jian-Feng Wang, Chen-Teng Yang, Guo-Zhong Zhang, Bin Cong
{"title":"Advances in the Study of Cerebrocardiac Syndrome and Its Forensic Significance.","authors":"Jian-Feng Wang, Chen-Teng Yang, Guo-Zhong Zhang, Bin Cong","doi":"10.12116/j.issn.1004-5619.2024.440303","DOIUrl":"https://doi.org/10.12116/j.issn.1004-5619.2024.440303","url":null,"abstract":"<p><p>Cerebrocardiac syndrome refers to secondary cardiac damage caused by various craniocerebral injury lesions, which can exacerbate existing heart disease. In the practice of forensic pathology, cerebral-cardiac interaction is often ignored in cases of death with a short time after craniocerebral injury, especially those with underlying heart disease. This article reviews the pathogenesis of cerebrocardiac syndrome by summarizing recent research results of cerebrocardiac syndrome at home and abroad and discusses its significance in the field of forensic medicine. The aim is to provide assistance for forensic medicine practice of cerebrocardiac syndrome.</p>","PeriodicalId":12317,"journal":{"name":"法医学杂志","volume":"40 4","pages":"372-378"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
法医学杂志Pub Date : 2024-08-25DOI: 10.12116/j.issn.1004-5619.2022.420406
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