Fiziolohichnyi zhurnal最新文献_第5页

[DOXORUBICIN-INDUCED ALTERATIONS IN PRO-AND ANTIOXIDANT BALANCE AND THEIR CORRECTION BY CURCUMIN IN THE NEONATAL RAT CARDIOMYOCYTES CULTURE]. [阿霉素诱导的新生大鼠心肌细胞培养中亲、抗氧化平衡的改变及其姜黄素的纠正]。

Fiziolohichnyi zhurnal Pub Date : 2015-01-01 DOI: 10.15407/FZ61.05.090

O. Linnik, T. Drevytska, O. Gonchar, S. Chornyy, O. Kovalyov, I. Mankovska

{"title":"[DOXORUBICIN-INDUCED ALTERATIONS IN PRO-AND ANTIOXIDANT BALANCE AND THEIR CORRECTION BY CURCUMIN IN THE NEONATAL RAT CARDIOMYOCYTES CULTURE].","authors":"O. Linnik, T. Drevytska, O. Gonchar, S. Chornyy, O. Kovalyov, I. Mankovska","doi":"10.15407/FZ61.05.090","DOIUrl":"https://doi.org/10.15407/FZ61.05.090","url":null,"abstract":"It was studied the effect of doxorubicin on the HIF system and the pro-antioxidant balance of neonatal cardiomyocytes as well as the possibility of the oxidative stress correcting using curcumin. It has been revealed that the expression of mRNA HIF-1α using doxorubicin at a dose of 0.5 μM was 2.9 ± 0.8 cu, so it decreased by 20% compared to control--3.6 ± 0.7 cu (P < 0.05). The level of expression of the HIF target gene PDK-1 also significantly decreased (4 times). During the incubation with doxorubicin, the number of live cells decreased by 50.4% relative to control. And after using doxorubicin and curcumin together, the percentage of dead cells decreased by 7,7 compared to doxorubicin only. Doxorubicin intoxication led to a significant increase in the secondary products of lipid peroxidation (TBARS) in cardiomyocytes by 3.6 times and hydrogen peroxide by 64%. Prolonged incubation with doxorubicin reduced the enzymatic activity of Mn-SOD by 32%, while catalase activity increased by 72% compared to control. Adding of curcumin to the cardiomyocyte cell culture contributed to increasing of the Mn-SOD activity by 14%, catalase--by 23%. The level of TBARS increased by 1,4 times compared with the control, and the level of H2O2 increased by 20%. The joint use of doxorubicin and curcumin resulted in a significant reduction of free radical oxidation unlike effect of doxorubicin per se. Specifically, there was lessening of TBARS and H2O2 (at 56.7 and 18.4% respectively), while decreasing of the catalase hyperactivation (19%) and rising of the Mn-SOD activity (35%).","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81035464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[ENERGETIC AND ANTIOXIDANT STATUS OF RAT LIVER MITOCHONDRIA DURING HYPOXIA-REOXYGENATION OF DIFFERENT DURATION]. [不同缺氧-复氧持续时间大鼠肝脏线粒体的能量和抗氧化状态]。

Fiziolohichnyi zhurnal Pub Date : 2015-01-01 DOI: 10.15407/FZ61.06.035

O. Gonchar, V. Nosar, L. V. Bratus, I. Tymchenko, N. Steshenko, I. Mankovska

{"title":"[ENERGETIC AND ANTIOXIDANT STATUS OF RAT LIVER MITOCHONDRIA DURING HYPOXIA-REOXYGENATION OF DIFFERENT DURATION].","authors":"O. Gonchar, V. Nosar, L. V. Bratus, I. Tymchenko, N. Steshenko, I. Mankovska","doi":"10.15407/FZ61.06.035","DOIUrl":"https://doi.org/10.15407/FZ61.06.035","url":null,"abstract":"Dynamics of changes in activity and protein expression of antiradical (MnSOD), glutathione-dependent (glutathione peroxidase, glutathione reductase) and NADP⁺-generated (isocitrate dehydrogenase) enzymes as well as in the energy metabolism indeces in rat liver mitochondria under hypoxia- reoxygenation of different duration (1, 3, 7 14 days) were studied. Prolonged hypoxia-reoxygenation was characterized by phase changes of the corticosterone concentration in rat blood, which corresponded to the changes in energy metabolism as well as in pro- and antioxidant balance in rat liver mitochondria. It has been shown that short-term (1 day) hypoxia-reoxygenation (5% O2 in the gas mixture) led to an increase in the blood corticosterone concentration and a significant activation of oxidative processes and energy metabolism in rat liver mitochondria, the intensity of which was reduced to 3rd day. Long- term hypoxia--reoxygenation (7-14th days) led to the gradual depletion of the organism adaptive capabilities, as evidenced by a significant decline in the blood corticosterone concentration, an increase in the content of secondary products of lipid peroxidation, an imbalance in pro- and antioxidant reactions and reduction of energy capacity in liver cells mitochondria. It has been shown that the glutathione peroxidase protein expression and enzymatic activity increased constantly during the whole experimental period and correlated positively with the level of H₂O₂. The amount of Mn-SOD protein as well as it's enzymatic activity was lower in the first seven days of experiment, and it was increased in consequent days up to the control level on 14thday. Increased activity of glutathione peroxidase, glutathione reductase and NADP+⁺dependent isocitrate dehydrogenase during prolonged hypoxia - eoxygenation indicates that glutathione- and NADPH-generating enzymes, were actively involved in the antioxidant protect.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83554615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[THE EFFECT OF DIETARY RESTRICTION DURING DEVELOPMENT OF DROSOPHILA MELANOGASTER ON THE ACTIVITY OF ANTIOXIDANT SYSTEM ENZYMES]. [黑腹果蝇发育期间饮食限制对抗氧化系统酶活性的影响]。

Fiziolohichnyi zhurnal Pub Date : 2015-01-01 DOI: 10.15407/FZ61.06.114

O. Zabuga, A. Koliada, V. Kukharskyy, A. I. Bazhynova, A. Vaiserman

{"title":"[THE EFFECT OF DIETARY RESTRICTION DURING DEVELOPMENT OF DROSOPHILA MELANOGASTER ON THE ACTIVITY OF ANTIOXIDANT SYSTEM ENZYMES].","authors":"O. Zabuga, A. Koliada, V. Kukharskyy, A. I. Bazhynova, A. Vaiserman","doi":"10.15407/FZ61.06.114","DOIUrl":"https://doi.org/10.15407/FZ61.06.114","url":null,"abstract":"In the previous study we demonstrated that dietary restriction only at the development stage of Drosophila melanogaster may impact the life span of adult flies. It was important that we didn't use qualitative (restriction of proteins or other macro- or microelements) and not a calorie restriction as well, but quantitative dietary restriction that was the proportional reduction of all food components in the larval medium. In the situations when the larvae were reared in the medium types, that contained protein and carbohydrate components in concentrations of 90-10% of food components compared to the standard one (100%), the males were characterised with the significant increase in the maximum life span. The average life span was also increased, but only in those male individuals that developed in the medium types, that contained 50% and 60% of food components compared to controls. Such an effect we haven't detected in the female flies. To study the biochemical changes associated with the physiological effects we have determined the activity of the antioxidant enzymes--superoxide dismutase (SOD) and catalase. In the male flies the 50% dietary restriction implemented during the development has led to the significant increase in a SOD and catalase activity. Also the flies of both sexes reared in the medium with the 50% of food components have been characterised with the reduction in the accumulation of glycation end products. According to these results, we suggest that the changes in the activity of antioxidant enzymes may play a role in the increase of the flies life span caused by the dietary restriction during the development.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77525277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

[NADPH-DIAPHORASE REACTIVITY IN THE VENTRAL HORN OF THE FELINE SPINAL CORD DURING ACUTE MUSCLE INFLAMMATION]. [急性肌肉炎症期间猫脊髓前角nadph-diaphorase的反应性]。

Fiziolohichnyi zhurnal Pub Date : 2015-01-01 DOI: 10.15407/FZ61.06.046

Ye. P. Man’kovskaya, A. V. Maznychenko, N. O. Pil'kevych, V. O. Maisky, O. V. Vlasenko, O. Dovgan

{"title":"[NADPH-DIAPHORASE REACTIVITY IN THE VENTRAL HORN OF THE FELINE SPINAL CORD DURING ACUTE MUSCLE INFLAMMATION].","authors":"Ye. P. Man’kovskaya, A. V. Maznychenko, N. O. Pil'kevych, V. O. Maisky, O. V. Vlasenko, O. Dovgan","doi":"10.15407/FZ61.06.046","DOIUrl":"https://doi.org/10.15407/FZ61.06.046","url":null,"abstract":"The aim of this research was to reveal the changes in the NADPH-d reactivity in the lumbal spinal cord (L6/L7) of cats with unilateral acute myositis of the mm. gastrocnemius-soleus after intramuscular injections of carrageenan. The effect of unilateral muscle inflammation was expressed in a significant increase in the number of NADPH-d-reactive neurons in ipsilateral and contralateral intermediate (lamina VII; 17.62 ± 2.7 and 20.67 ± 13.3) and medial (lamina VIII; 7.3 ± 1.9 and 6.0 ± 2.1 respectively) zones of the ventral horns. However, a clear decline of the reactive cells was recorded on the ipsilateral side within the area around the central canal (lamina X). An increase in the NADPH-d reactivity within the ventral horns on both sides on the spinal cord and the induction of such reactivity (contralaterally) in large multipolar neurons localized in the dorsal part of the intermediate zone were revealed in cats with unilateral acute muscle inflammation. It is hypothesized, that during acute myositis, plastic changes in different layers of the dorsal and ventral horns activate the processes of disinhibition due to an increase in the number of NOS-containing/NADPH-d-reactive neurons in the spinal gray matter.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81147455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[THYMIC HORMONES, ANTIOXIDANT ENZYMES AND NEUROGENESIS OF BULBUS OLFACTORIUS IN RATS WITH PARKINSONISM: THE EFFECT OF MELATONIN]. [帕金森大鼠胸腺激素、抗氧化酶和嗅球神经发生:褪黑素的影响]。

Fiziolohichnyi zhurnal Pub Date : 2015-01-01 DOI: 10.15407/FZ61.05.035

I. Labunets, Talanov Sa, Vasilyev Rg, A. Rodnichenko, N. Utko, Kyzminova Ia, Kopjak Bs, Podjachenko Ev, V. Sagach, G. Butenko

{"title":"[THYMIC HORMONES, ANTIOXIDANT ENZYMES AND NEUROGENESIS OF BULBUS OLFACTORIUS IN RATS WITH PARKINSONISM: THE EFFECT OF MELATONIN].","authors":"I. Labunets, Talanov Sa, Vasilyev Rg, A. Rodnichenko, N. Utko, Kyzminova Ia, Kopjak Bs, Podjachenko Ev, V. Sagach, G. Butenko","doi":"10.15407/FZ61.05.035","DOIUrl":"https://doi.org/10.15407/FZ61.05.035","url":null,"abstract":"The adult rats received both neurotoxin 6-hidroxidophamine and neurotoxin and melatonin. It was investigated a link between the disturbances of the brain antioxidant enzymes activity and thymic endocrine function, as possible pathogenic factors of parkinsonism, with changes in the number of neural stem cells (NSC) in the bulbus olfactorius. Rats with motor asymmetry in the apomorphine test and significant damage of the dopaminergic neurons in the-substantia nigra have decreased levels of superoxide dismutase, catalase and glutathione peroxidase activities in striatum (1.3-1.4 times) and blood thymulin content (8 times) compared to control group. On the contrary, examined indices were not changed in rats without motor asymmetry and correspondingly partly damaged neurons. The number of nestin(+)-cells in the bulbus olfactorius of rats without motor asymmetry increased from 91.2% to 99.3% and remained unchanged after melatonin administration course (10 mg/kg during 18 days). Melatonin administration resulted in the decrease in the number of nestin(+)-cells along with significant elevation of the decreased antioxidant enzymes activity and blood thymulin content in rats with circulatory movements. Possibilities of the enhancement of NSC differentiation in bulbus olfactorius into neuronal direction in such animals has been discussed. The conclusion about the potential use of melatonin as a neuroprotector in parkinsonism therapy has been made.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83272984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Phase changes of energy metabolism during adaptation to immobilization stress]. [适应固定应激过程中能量代谢的相位变化]。

Fiziolohichnyi zhurnal Pub Date : 2014-10-10 DOI: 10.15407/fz60.05.023

V. Portnichenko, V. Nosar, O. Honchar, H. V. Opanasenko, I. D. Hlazyrin, I. M. Man'kovs'ka

{"title":"[Phase changes of energy metabolism during adaptation to immobilization stress].","authors":"V. Portnichenko, V. Nosar, O. Honchar, H. V. Opanasenko, I. D. Hlazyrin, I. M. Man'kovs'ka","doi":"10.15407/fz60.05.023","DOIUrl":"https://doi.org/10.15407/fz60.05.023","url":null,"abstract":"In stress, it was showed the organ and tissue changes associated with damage by lipid peroxides, and the disrupted barrier function. As a consequence, it was to lead to a syndrome of \"stress-induced lung\" and violation of oxygen delivery to the tissues and hypoxia. Purpose of the study was to investigate the dynamics of changes in gas exchange, blood glucose, body temperature, oxidant and antioxidant system activity, as well as mitochondrial respiration by Chance under the influence of chronic stress (6-hour immobilization daily for 3 weeks). It was identified 4 phase changes of energy metabolism in the dynamics of chronic stress. In the first phase, hypomethabolic, instability oxidative metabolism, decreased oxidation of NAD-dependent substrates, significant elevation of FAD-dependent substrates oxidation and low MRU were found. The activity of superoxide dismutase (MnSOD) was increased; it was occurred on a background low activity of glutathione peroxidase, and of misbalanced antioxidant system. After seven immobilizations, second phase-shift in energy metabolism, was observed, and then the third phase (hypermetabolic) started. It was characterized by gradual increase in oxidative metabolism, the restoration of oxidation of NAD-dependent substrates, MRU, as well as optimizing balance of oxidant and antioxidant systems. The fourth phase was started after 15 immobilizations, and characterized by the development of adaptive reactions expressed in increased tolerance of energy metabolism to the impact of immobilization. The results are correlated with changes in the dynamics of blood corticosterone. Thus, it was found the phase character of the energy metabolism rebuilding during the chronic stress.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89574802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The effect of mild hypobaric hypoxia regime on expression of factor induced by hypoxia in the rat neocortex]. [轻度低压缺氧对大鼠新皮层缺氧诱导因子表达的影响]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-15 DOI: 10.15407/FZ59.06.111

M. Sidorova, E. Rybnikova, A. Churilova, V. Portnichenko, M. O. Sanoĭlov

引用次数: 4

[Pattern of neuronal expression of transcription factors NF-kappaB under different modes of hypobaric hypoxia]. [不同低压缺氧模式下神经元转录因子NF-kappaB的表达规律]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-15 DOI: 10.15407/FZ59.06.132

M. Samoilov, A. Churilova, T. Glushchenko, K. Baranova

{"title":"[Pattern of neuronal expression of transcription factors NF-kappaB under different modes of hypobaric hypoxia].","authors":"M. Samoilov, A. Churilova, T. Glushchenko, K. Baranova","doi":"10.15407/FZ59.06.132","DOIUrl":"https://doi.org/10.15407/FZ59.06.132","url":null,"abstract":"Transcription factor NF-kappaB plays a pivotal role in mechanisms of brain neuron survival and degeneration under injurious stimuli, first of all different types of hypoxia. In the present work, using quantitative immunohystochemistry, we provide analysis of expression of different subunits of NF-kappaB (p65 and c-Rel) in the rat neocortex in response to severe injurious hypobaric hypoxia (HH) or after a single or multiple sessions of mild protective HH. Severe hypoxia (SH), resulting in loss of brain neurons, has no effect on the level of expression of p65 but suppresses expression of c-Rel. Multiple (but not single one) trials of preconditioning using mild HH which reduce neuronal damage promote p65 expression and prevent suppression of c-Rel level after SH. Triple session of mild HH itself when applied as a preconditioning stimulus upregulate expression of both subunits, while single administration or sixfold trials has no effect on the level of immunoreactivity of both subunits. The revealed peculiarities of the expression of p65 and c-Rcl implies that these subunits of NF-kappaB appear to contribute to the mechanisms of brain tolerance to SH.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74263592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic predisposition to essential hypertension in children: analysis of 17 single nucleotide polymorphisms. 儿童原发性高血压的遗传易感性:17个单核苷酸多态性分析。

Fiziolohichnyi zhurnal Pub Date : 2014-01-15 DOI: 10.1615/INTJPHYSPATHOPHYS.V5.I4.40

S. Goncharov, V. Gurianova, D. Stroy, T. Drevytska, S. P. Kaplinskii, E. A. Nastenko, M. Litvinenko, R. V. Terletskiy, M. Khaitovych, O. Moibenko, V. Dosenko

引用次数: 3

[Involvement of transcriptional factor induced by hypoxia in the neuronal mechanisms of adaptation to psychoemotional and hypoxic stress]. [缺氧诱导的转录因子参与神经元对心理和缺氧应激的适应机制]。

Fiziolohichnyi zhurnal Pub Date : 2014-01-15 DOI: 10.15407/FZ59.06.088

E. Rybnikova, K. Baranova, T. Glushchenko, O. V. Vetrovoi, M. Sidorova, V. Portnichenko

{"title":"[Involvement of transcriptional factor induced by hypoxia in the neuronal mechanisms of adaptation to psychoemotional and hypoxic stress].","authors":"E. Rybnikova, K. Baranova, T. Glushchenko, O. V. Vetrovoi, M. Sidorova, V. Portnichenko","doi":"10.15407/FZ59.06.088","DOIUrl":"https://doi.org/10.15407/FZ59.06.088","url":null,"abstract":"Using quantitative immunohistochemistry, neuronal expression of alpha-subunit of the transcriptional factor HIF-1 in hippocampus and neocortex of rats in response to pathogenic psychoemotional (model of posttraumatic stress disorder, PTSD) and hypoxic (severe hypobaric hypoxia, 180 Torr, 3 h), as well as to neuroprotective exposures to hypoxic pre- and postconditioning has been studied. Elongated overexpression of HIF-1alpha in hippocampus and neocortex of rats in response to the psychoemotional stress in PTSD paradigm, but not hypoxic stress, has been observed. Hypoxic pre- and postconditioning with mild hypobaric hypoxia (360 Torr, 2 h, 3 trials spaced at 24 h), those induced adaptation to the psychoemotional stress, abolished the elongated HIF-1alpha overexpression. Hypoxic postconditioning which improved structure and functional rehabilitation following severe hypoxic stress up-regulated HIF-1alpha expression in the brain neurons of rats survived severe hypoxia. The findings indicate that transcription factor HIF-1 is particularly involved in the processes of adaptation/ maladaptation to the action of injurious stresses, but its role depends upon the nature of stressor.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77483628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5