Allergy最新文献

{"title":"Recombinant Hypoallergenic Cat Allergy Vaccines.","authors":"Daria Trifonova, Mirela Curin, Margarete Focke-Tejkl, Zicheng Liu, Kristina Borochova, Pia Gattinger, Marianne van Hage, Hans Grönlund, Renata Kiss, Huey-Jy Huang, Walter Keller, Ksenja Riabova, Antonina Karsonova, Michael Kundi, Inna Tulaeva, Daria Fomina, Alexander Karaulov, Rudolf Valenta","doi":"10.1111/all.16542","DOIUrl":"https://doi.org/10.1111/all.16542","url":null,"abstract":"<p><strong>Background: </strong>Molecular forms of allergen-specific immunotherapy (AIT) for cat allergy are needed. Fel d 1, Fel d 4, and Fel d 7 are the most important cat allergens.</p><p><strong>Methods: </strong>IgE epitopes of Fel d 4 and Fel d 7 were mapped by blocking allergic patients' IgE binding with allergen peptide-specific antisera. Five recombinant fusion proteins (PreS-Cat 1-PreS-Cat 5) each containing hepatitis B virus (HBV)-derived PreS as an immunological carrier and non-allergenic peptides from the IgE binding sites of Fel d 1, Fel d 4, and Fel d 7 were expressed in Escherichia coli, purified, and characterized by mass spectrometry, circular dichroism (CD), and size exclusion chromatography. ImmunoCAP and basophil activation experiments demonstrated the hypoallergenic activity of PreS-Cat 1-5. The ability of PreS-Cat 1-5 to induce IgE-blocking antibodies in rabbits was compared to three licensed allergen extract-based AIT vaccines. PreS-Cat 1-5-specific IgG antibodies were tested for inhibition of allergen-specific IgE binding and specific basophil activation. T cell activation and induction of specific cytokine secretion by PreS-Cat proteins were compared with cat allergens in PBMC cultures.</p><p><strong>Results: </strong>Recombinant hypoallergenic, biochemically and structurally defined PreS-Cat 1-5 were obtained. Two subcutaneous immunizations of rabbits with PreS-Cat 1-5 induced equal (Fel d 1) or better (Fel d 4 and Fel d 7) antibodies (PreS-Cat 5 > PreS-Cat 1 > PreS-Cat 3) blocking allergic patients' IgE binding to cat allergens than six to fifteen immunizations with allergen extract-based vaccines. PreS-Cat-specific antibodies strongly inhibited specific basophil activation. PreS-Cat 5 > PreS-Cat 1 induced significantly more IL-10 in cultured PBMCs from cat allergic patients than cat allergens.</p><p><strong>Conclusions: </strong>PreS-Cat 5 and PreS-Cat 1 are highly promising molecular vaccine candidates for AIT of cat allergy, combining Fel d 1-, Fel d 4-, and Fel d 7-peptides in single PreS fusion proteins.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Adventitial Stromal Cells and Myofibroblasts Recruit Pro- and Anti-Inflammatory Immune Cells in Allergic Airway Inflammation\".","authors":"","doi":"10.1111/all.16549","DOIUrl":"https://doi.org/10.1111/all.16549","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NLRC4 Regulates Th2 Differentiation in Mice With Allergic Airway Inflammation Induced by House Dust Mite.","authors":"So-Won Pak, Woong-Il Kim, Se-Jin Lee, Junhyeong Lee, Min-Jung Park, Jong-Hwan Park, Jong-Choon Kim, Taesoo Kim, Joong-Sun Kim, Yun Hee Kim, In-Sik Shin","doi":"10.1111/all.16550","DOIUrl":"https://doi.org/10.1111/all.16550","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOP10-SCAR: A Global Pharmacovigilance Study on Medications Most Frequently Related to Severe Cutaneous Adverse Reactions.","authors":"Jaehyeong Cho, Hyesu Jo, Jaeyu Park, Jeongseon Oh, Tae Hyeon Kim, Kyeongmin Lee, Hayeon Lee, Jinyoung Jeong, Sooji Lee, Michael Miligkos, Nikolaos G Papadopoulos, Dong Keon Yon","doi":"10.1111/all.16544","DOIUrl":"https://doi.org/10.1111/all.16544","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiology of Chronic Urticaria in Cape Town, South Africa: A Review of Two Tertiary Referral Centers","authors":"Cascia Day, Mimi Deetlefs, Lovemore Mapahla, Yejin Jang, Qiqa Gusha‐Mhlekude, Sicelo Ntuli, Balican Hlungwani, Susanna Kannenberg, Emma Kruger, Ndapewa Ambondo, Willem Visser, Thuraya Isaacs, Rannakoe Lehloenya, Jonny Peter","doi":"10.1111/all.16547","DOIUrl":"https://doi.org/10.1111/all.16547","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"12 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical Distribution and Quantification of IgE Secretion in Mouse Models of House Dust Mite Allergy","authors":"Paul Haase, Angga Perima, Venkat Raman Ramnarayan, Patrick England, Bruno Iannascoli, Andre Limnander, Seblewongel Asrat, Andrea Vecchione, Jamie M. Orengo, Matthew A. Sleeman, Pierre Bruhns","doi":"10.1111/all.16546","DOIUrl":"https://doi.org/10.1111/all.16546","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"4 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta‐Analysis of PQ Grass 27600 SU Efficacy and Quality of Life From Phase III Trials","authors":"Stefan Zielen, Jonathan A. Bernstein, Oliver Pfaar, Lawrence DuBuske, Ralph Mösges, Sven Becker, Fiona Sellwood, Matthias F. Kramer, Murray Skinner, Pieter‐Jan de Kam","doi":"10.1111/all.16535","DOIUrl":"https://doi.org/10.1111/all.16535","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"2 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological In Vitro Assay for Quantification of Adjuvanted Allergoids","authors":"S. Schlünder, J. Echternach, D. Bartel, V. Mahler, M. D. Mühlebach, F. Führer","doi":"10.1111/all.16543","DOIUrl":"https://doi.org/10.1111/all.16543","url":null,"abstract":"BackgroundMany IgE‐mediated allergic disorders can be treated with allergen immunotherapy (AIT). In order to improve safety and efficacy, some AIT products contain allergen extracts which are chemically cross‐linked to generate allergoids and are adsorbed to aluminum hydroxide adjuvant. The modification and adsorption impair accessibility of the protein and quantification of the allergoid content.MethodsAn ELISA‐like assay to quantify the allergoid content in adjuvanted grass pollen allergoid AIT products (from here on called: AIT drug products; AIT‐DPs) was developed using a fluorescence detection system. The high density of the aluminum hydroxide particles enabled pelleting the antigen complexes by centrifugation. Rabbit anti‐grass pollen allergoid sera or a mouse anti‐Phl p 5 monoclonal antibody (mAb) was used as the primary antibody. Protein content of the samples was quantified by nitrogen analysis.ResultsHigh specificity of the primary antibodies was confirmed by isoelectric focusing, gel‐electrophoresis, and immunoblotting. Performance of the allergoid content assay was demonstrated in grass pollen AIT‐DPs with high specificity and low/absent cross‐reactivity with tree pollen or mite AIT‐DPs. It was used to confirm batch‐to‐batch consistency and allergoid content of distinct grass pollen AIT‐DPs. Overall, in relation to their total protein content, the allergoid content ranged between 0.8 and 2.1 relative to an in‐house reference for all grass pollen AIT‐DPs, whereas use of mAb revealed product‐specific differences in the Phl p 5 amount. Additionally, the assay detects product alteration by heat stress.ConclusionThe described assay is suitable to quantify the allergoid content and quality of allergoids in complex with aluminum hydroxide. It is suitable for animal‐free final product testing in vitro, for example, for batch release to ensure the quality of AIT‐DPs.","PeriodicalId":122,"journal":{"name":"Allergy","volume":"82 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head-To-Head Comparison of Biologic Efficacy in Asthma: What Have We Learned?","authors":"Brian J Lipworth, Robert Greig, Rory Chan, Chris RuiWen Kuo, Catherine Jackson","doi":"10.1111/all.16537","DOIUrl":"https://doi.org/10.1111/all.16537","url":null,"abstract":"<p><p>We performed an in-depth appraisal of indirect head-to-head comparisons of biologics approved for asthma, including anti-IL5/5Rα (mepolizumab, benralizumab), anti-IL4Rα (dupilumab), anti-TSLP (tezepelumab) and anti-IgE (omalizumab), which was neither a systematic review nor a meta-analysis. A crude evaluation of 95% CI's for rate ratios which excluded unity revealed greater overall reductions in annualised exacerbations with dupilumab versus either mepolizumab or benralizumab and also with tezepelumab versus benralizumab. Furthermore in patients with eosinophils ≥ 300/μL exacerbation rates were lower for tezepelumab, dupilumab and mepolizumab versus benralizumab; and with eosinophils< 150/μL for tezepelumab versus dupilumab. For lung function, no overall differences in FEV1 response were observed between drugs where there was considerable heterogeneity of overlapping 95% CI's. Dupilumab was superior to benralizumab for oscillometry-derived peripheral lung resistance and compliance, as well as for attenuation of mannitol airway hyperresponsiveness. There were no differences in asthma control or quality of life scores where the effect sizes were small, along with wide overlaps in 95% CI's. There is an unmet need for prospective pragmatic randomised controlled trials to directly compare biologics, especially to assess clinical remission in both type 2 high and low asthma patients. Real-life studies might also evaluate complete remission with different biologics to include outcomes such as inhaled corticosteroid sparing, small airways dysfunction using oscillometry, abolition of airway hyperresponsiveness and to assess mucus plugging and remodelling as wall thickening with imaging.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, Epidemiological, Virological Characteristics and Outcomes of 286 Patients Infected With Monkeypox Virus in China.","authors":"Hankun Hu, Yang Zheng, Lianguo Ruan, Yamin Liu, Yeqing Tong, Yaokai Chen, Ke Liang, Li Zhou, Wu Chen, Yixin Hu, Wei Song, Feng Lv, Yaodong Ping, Kaiwen Fang, Nan Zhang, Hongxia Wei, Cezmi A Akdis, Ping Ma, Yadong Gao","doi":"10.1111/all.16540","DOIUrl":"https://doi.org/10.1111/all.16540","url":null,"abstract":"<p><strong>Background: </strong>Monkeypox virus (mpox) outbreak since 2022 has already constituted a public health emergency of international concern. A comprehensive study on epidemiological features, symptoms and signs, complications, sequelae, and clinical outcomes has been lacking. Accordingly, we performed a large-scale multicenter study to comprehensively summarize the clinical, epidemiological, behavioral, laboratory, virological characteristics, and treatment-related outcomes of mpox in China.</p><p><strong>Method: </strong>In this multicenter hospital-based retrospective study, demographic information, clinical characteristics, laboratory results, hospitalization records, prescribed medications, and clinical outcomes were extracted from the electronic medical records of 286 confirmed mpox cases from five regions of China.</p><p><strong>Result: </strong>An approximately 1:1 ratio of HIV-positive (52.1%) and HIV-negative was found, with an overall median age of 32 years (IQR 27-37). Most patients were male (99.3%), lived in urban areas (86.0%), single (72.6%), and had a college degree or higher (62.0%). Men having sex with men (MSM) was the predominant sexual orientation (83.0%), and sexual contact was the most likely mode of exposure (85.4%). 17 (9.6%) patients reported a history of smallpox vaccination. Hundered and seven patients had at least one co-morbid other sexually transmitted infection. Fourteen patients had a self-reported history of allergy. Significant differences were found between HIV-negative and HIV-positive mpox in the proportion of MSM, anal and perianal pain, levels of AST, CRP, CD4+ T-cell, CD8+ T-cell, NK-cell levels, and clinical outcomes.</p><p><strong>Conclusion: </strong>MSM individuals with an interquartile age range of 27-37 years, particularly from coastal or developed regions of China, were identified as \"main affected population\" for mpox prevention and control. HIV infection may contribute to more severe mpox manifestations, characterized by elevated AST and CRP levels, reduced CD4 + T-cell and NK-cell counts, and unfavorable clinical outcomes with increased mortality.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}