Allergy最新文献

{"title":"An Extended Transcriptomic Landscape of Human Skin Mast Cells: Unveiling Lineage-Specific Noncoding RNA Dynamics in FcεRI Activation.","authors":"Gürkan Bal, Kristin Franke, Stefan Frischbutter, Shiva Raj Tripathi, Kayoko Yasuzawa, Jordan A Ramilowski, Michihira Tagami, Takeya Kasukawa, Michiel de Hoon, Jay W Shin, Piero Carninci, Torsten Zuberbier, Magda Babina","doi":"10.1111/all.70558","DOIUrl":"https://doi.org/10.1111/all.70558","url":null,"abstract":"<p><strong>Background: </strong>Mast cells (MCs) are crucial in allergic and inflammatory responses, including in skin disorders. However, the transcriptional landscape of resting and stimulated skin MCs and the role of long non-coding RNAs (lncRNAs) remain poorly characterized.</p><p><strong>Objective: </strong>To define FcεRI-induced gene and promoter regulation in primary mast cells from human foreskin (n = 5 cultures) and breast skin (n = 2 cultures), assess tissue-origin effects, identify novel lncRNAs, and evaluate MALAT1 function.</p><p><strong>Methods: </strong>Skin-derived MCs were stimulated by FcεRI aggregation and subjected to Low Quantity single-strand Cap Analysis of Gene Expression (LQ-ssCAGE) sequencing. MALAT1 knockdown was achieved via antisense oligonucleotides. Functional evaluations included flow cytometry, caspase-3 activity, cytokine secretome studies, and histamine release assays.</p><p><strong>Results: </strong>FcεRI cross-linking induced considerable changes, with over 5000 genes and 6400 promoters being differentially regulated, also encompassing many novel MC-enriched lncRNAs, e.g., CATG00000075668, CATG00000072979, and CATG00000048788. Selected cytokines were among the strongest responders, and their pattern was largely reproduced at the secretome level (e.g., GM-CSF, IL-5, TNF, LIF). Cell surface receptors like NTRK were likewise boosted. Moderate differences were noted between MCs from breast and foreskin, mainly affecting cytokines and tryptase isoenzymes. MALAT1 influenced the transcription of only few genes, but contributed to FcεRI-triggered histamine release.</p><p><strong>Conclusion: </strong>FcεRI aggregation triggered widespread transcriptional reprogramming in skin MCs, also encompassing novel MC-selective lncRNAs. The skin source made a modest contribution to FcεRI responsiveness, while MALAT1 fine-tuned MC function. The study shows that lncRNAs can be successfully manipulated in primary tissue MCs to further determine the function of this new class of biomolecules.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":11.3,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149339980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Changes and Their Effect on the Airway Microbiome: Implications for Airway Diseases.","authors":"Georgios Rentzos, Evangelia Fouka, Nikolaos Lazarinis, Theo Gülen, Patricia Ramos-Ramirez, Lauren Flynn, Stelios Loukides, Paraskevi Xepapadaki, Nikolaos G Papadopoulos, Anders Lindén, Apostolos Bossios","doi":"10.1111/all.70552","DOIUrl":"https://doi.org/10.1111/all.70552","url":null,"abstract":"<p><p>Obstructive airway diseases, including asthma and chronic obstructive pulmonary disease (COPD), represent the two major chronic airway inflammatory diseases. In both conditions, the airways are exposed to a variety of microbial and environmental stimuli. A healthy airway microbiome is characterized not only by microbial diversity but also by the absence of significant quantities of pathogens, and is signified by ecological stability, functional resilience, and balanced interactions with the host immune system. Although microbial diversity is often considered a marker of respiratory health, its association with disease depends on the anatomical site, host factors, and environmental exposures. Thus, microbial diversity alone should not be used as a universal marker of airway health-the occurrence of pathogens and the organ compartment are also factors that are important to consider. Disruption of this microbial homeostasis, known as dysbiosis, may involve reduced diversity of the commensal flora as well as increased quantity of key pathogens, and this condition is believed to stimulate inflammation that is associated with the progression of asthma and COPD, including exacerbations. Environmental changes, including air pollution, climate change, and urbanization, can disrupt microbial balance by reducing diversity and increasing the prevalence of pathogens. Several mechanisms contribute to this interaction, including damage to the airway epithelium, impaired mucociliary clearance, oxidative stress, and alterations in innate and adaptive immune responses. This review examines the interplay between external environmental factors and the airway microbiome, and how they affect airway obstructive diseases, asthma, and COPD to understand their immunopathology, and to improve disease prevention and treatment approaches. This is an area of great interest, and further research is required to elucidate these interactions so we can better help our patients.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":11.3,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Omics Analysis Reveals Vitamin D Metabolism, Hyper-IgE Genes, and Epithelial Barrier Dysfunction in Hazelnut Allergy.","authors":"Alexander C S N Jeanrenaud, Aleix Arnau-Soler, Ahla Ghauri, Ingo Marenholz, Philipp Mertins, Matthias Ziehm, Kirsten Beyer, Margitta Worm, Young-Ae Lee","doi":"10.1111/all.70525","DOIUrl":"https://doi.org/10.1111/all.70525","url":null,"abstract":"<p><strong>Background: </strong>Hazelnut allergy is a major cause of food-induced anaphylaxis yet remains poorly defined at the molecular level. We aimed to identify molecular differences between individuals with primary hazelnut allergy and nonallergic controls by investigating a comprehensive spectrum of omics profiles in immune cells.</p><p><strong>Methods: </strong>We analysed DNA methylation, transcriptomic, and proteomic profiles in hazelnut-stimulated and unstimulated immune cells.</p><p><strong>Results: </strong>Across analyses, we identified 87 differentially methylated signatures, 125 unique differentially expressed genes, and 11 unique differentially secreted proteins associated with hazelnut allergy. DNA methylation signatures were largely preserved irrespective of antigen stimulation, indicating stable epigenetic remodelling. Key findings implicated ZNF341, associated with a rare monogenic hyper-IgE syndrome, and ARL2, both linked to STAT3-mediated IgE dysregulation. We identified a hypermethylated region overlapping TH2LCRR in the cytokine gene cluster, suggesting an epigenetic mechanism contributing to IL-5 and IL-13 upregulation. Antigen stimulation was required to reveal hazelnut-specific transcriptional and proteomic signals. Integration of these data demonstrated that IL-5 expression could distinguish both groups. Moreover, integrated analysis of all multi-omics data revealed prominent innate immune activation programs involving antimicrobial, antiviral, and cytokine-mediated inflammatory responses. Finally, we identified signals in epithelial barrier genes of the gut and skin (TRIM31, TRIM40, CDSN), activation of the vitamin D pathway (CYP27B1, IL32), and nominated additional signals (PHACTR1, MFHAS1, SPRED2, NSMCE1-DT) for follow-up.</p><p><strong>Conclusion: </strong>This study provides the first antigen-specific multi-omics characterization of hazelnut allergy, confirming coordinated alterations in type 2-immunity and uncovering novel links to monogenic hyper-IgE syndrome, innate inflammatory pathways, activation of vitamin-D pathways, and gut/skin barrier genes, yielding a catalogue of candidate biomarkers for mechanistic studies and prospective validation.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":11.3,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct Circulating Eosinophil Phenotype in Active Eosinophilic Granulomatosis With Polyangiitis","authors":"Thomas Villeneuve, Florian Bonnafous, Grégory Pugnet, Edouard Leveque, Stanislas Faguer, Sébastien De Almeida, Grégoire Prévot, Léna Andrieux, Chloé Bost, Julien Maquet, Laurent Sailler, Laurent L. Reber, Laurent Guilleminault","doi":"10.1111/all.70548","DOIUrl":"https://doi.org/10.1111/all.70548","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"256 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COP31 : Climate Change, Environmental Hazards and the Adaptation‐Mitigation Dilemma","authors":"Lorenzo Cecchi, Gennaro D'Amato, Isabella Annesi‐Maesano","doi":"10.1111/all.70556","DOIUrl":"https://doi.org/10.1111/all.70556","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"20 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COP31 : The Climate Change Burden on Women and Children–From Vulnerability to Leadership and Action","authors":"Hatice Gulen, Amanda Ellis, Sandrine Dixson‐Declève","doi":"10.1111/all.70557","DOIUrl":"https://doi.org/10.1111/all.70557","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"13 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149304408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single‐Cell Omics Profiling Reveals Enhanced Heterogeneity of Cord Blood Basophils at Birth","authors":"Jing Hui Low, Xiaohui Man, Jocelyn Ong, Anna de Beer, Sofia Papavasileiou, Srinidhi Sudharson, Seohyun Kim, Yiping Fan, Hui Hua Liew, Alicia Tay, Shihui Foo, Shanshan W. Howland, Joakim S. Dahlin, Florent Ginhoux, Jerry Kok Yen Chan, Anand Kumar Andiappan","doi":"10.1111/all.70551","DOIUrl":"https://doi.org/10.1111/all.70551","url":null,"abstract":"Background Basophils are important mediators in the pathobiology of atopic diseases, yet the heterogeneity of basophils within the perinatal environment remains incompletely understood. In our study, we aimed to perform comprehensive profiling of paired maternal and cord blood basophils using a single‐cell omics approach to look into their distinct transcriptional signatures. Methods Basophils were isolated from four paired maternal‐cord blood samples at the time of delivery, including one mother who delivered monozygotic twins. Basophil characterization of maternal blood (MB) and cord blood (CB) was analyzed in flow cytometry. Single cell RNA sequencing (scRNA‐seq) was performed to further examine the basophils composition and heterogeneity in MB and CB. Results We observed that CB basophils have lower expression of FCER1A, and no cell‐bound or circulating IgE levels as compared to MB. Our scRNA‐seq data show that MB and CB basophils are different in both transcriptomic and functional profiles. CB basophils exhibit greater heterogeneity than MB. Low interindividual variability between twin CB basophil samples confirmed that the transcriptomic difference between MB and CB basophils is biologically meaningful. Trajectory pseudotime analysis revealed that CB basophils have an earlier pseudotime, potentially indicating a more naïve state. In addition, we demonstrated that CB basophils exhibit distinct functional capacities. Conclusion Our data identified protein and gene signatures in MB and CB basophils. We demonstrated that CB has greater heterogeneity than MB basophils, thereby highlighting the relevance of single cell profiling of basophils in the context of the maternal‐fetal interface.","PeriodicalId":122,"journal":{"name":"Allergy","volume":"18 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MRGPRX2 Antagonism in Chronic Spontaneous Urticaria: Phase 2 Randomized CALM ‐ CSU Study Results","authors":"Martin Metz, Manuel P. Pereira, Ana Giménez‐Arnau, Qi Yan, Christian Weyer, Terri Kim","doi":"10.1111/all.70547","DOIUrl":"https://doi.org/10.1111/all.70547","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"5 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the G20 to COP31 : Integrating Health Protection, Resilience, and Health Equity as a Key Measure of Climate Action","authors":"Ruby Pawankar, Kari C. Nadeau, Cezmi A. Akdis","doi":"10.1111/all.70550","DOIUrl":"https://doi.org/10.1111/all.70550","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"5 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149293018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgE ‐Binding Components in Fungi and Parasites: From Infection Severity to Allergy and Allergy Protection: An EAACI Position Paper","authors":"Uta Jappe, Marua Abu Risha, Ioana Agache, Claus Bachert, Barbara Bröker, Riccardo Castagnoli, Gabriele Gadermaier, Richard E. Goodman, Amir Guemari, Christiane Hilger, Alain Jacquet, Sabine Kespohl, Ludger Klimek, Annette Kuehn, Jonas Lidholm, Vera Mahler, Markus Ollert, Thomas A. Platts‐Mills, Monika Raulf, Peter Schmid‐Grendelmeier, Frieder Schmiedeke, Marianne van Hage, Joana Vitte, Josefina Zakzuk, Anna Pomés","doi":"10.1111/all.70543","DOIUrl":"https://doi.org/10.1111/all.70543","url":null,"abstract":"Human exposure to fungi and parasites (helminths, protozoa and ticks) can lead to the development and/or exacerbation of allergic/atopic diseases due to the production of IgE antibodies to components from these organisms. However, some of these IgE‐binding components are not currently listed in the official Allergen Nomenclature Database of the World Health Organization/International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub‐Committee. The aim of the Task Force (TF) BACALL (including members from this Sub‐Committee and four additional groups/committees) was to identify in the literature (PubMed up to October 2025) potential allergens from fungi and parasites and their reported clinical relevance, for possible future inclusion in the WHO/IUIS Allergen Nomenclature Database according to Sub‐Committee criteria. Forty‐nine IgE‐binding proteins from fungi and parasites were identified as potential allergen candidates. The IgE‐binding oligosaccharide α‐Gal was excluded because it is not proteinic, although it is associated with allergic reactions and anaphylaxis. Exposure to helminths outside the context of infection, such as ingestion of <jats:italic>Anisakis</jats:italic> ‐infected fish or vaccination against hookworms, can also elicit allergic reactions. The TF recommends that non‐conventional routes of exposure be considered in the identification of new allergens, and that future studies assess the clinical relevance of new allergens identified by the TF in larger populations.","PeriodicalId":122,"journal":{"name":"Allergy","volume":"15 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2026-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149281484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}