Allergy最新文献

{"title":"Correction to: D.J. Jackson, M.E. Wechsler, G. Brusselle, R. Buhl. Targeting the IL-5 pathway in eosinophilic asthma: A comparison of anti-IL-5 versus anti-IL-5 receptor agents. Allergy 2024;1-10. https://doi.org/10.1111/all.16346.","authors":"","doi":"10.1111/all.16541","DOIUrl":"https://doi.org/10.1111/all.16541","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow Cytometry-Assisted Analyses of Individual Human Basophils, Mast Cells and T Cells in the Diagnosis of Immediate Drug Hypersensitivity: A Review.","authors":"Jessy Elst, Christel Mertens, Michel Van Houdt, Marie-Line M van der Poorten, Athina L Van Gasse, Alessandro Toscano, Michiel Beyens, Daniel Yerly, Margo M Hagendorens, Vito Sabato, Oliver Hausmann, Didier G Ebo","doi":"10.1111/all.16548","DOIUrl":"https://doi.org/10.1111/all.16548","url":null,"abstract":"<p><p>Immediate drug hypersensitivity reactions (IDHRs) pose significant diagnostic challenges, often requiring potentially hazardous drug challenge testing (DCT). Flow cytometry-based cellular tests including the basophil activation test (BAT), the mast cell activation test (MAT) and the T cell activation test (TAT) offer promising alternatives to reduce DCT reliance. While these tests are still in development, they demonstrate potential to compete with skin tests by providing superior diagnostic performance and improved patient safety by reducing the need for DCT. Furthermore, it is encouraging that these flow cytometry-based tests are also suitable for challenging populations, such as children. Despite requiring specialised infrastructure, these tests have the potential to be cost-effective when performed in reference centres and may offer unique mechanistic insights into immediate drug hypersensitivity reactions. However, further research is needed to validate their reliability, address pharmaceutical-specific testing considerations, and potentially integrate them into clinical guidelines.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Hypoallergenic Cat Allergy Vaccines.","authors":"Daria Trifonova, Mirela Curin, Margarete Focke-Tejkl, Zicheng Liu, Kristina Borochova, Pia Gattinger, Marianne van Hage, Hans Grönlund, Renata Kiss, Huey-Jy Huang, Walter Keller, Ksenja Riabova, Antonina Karsonova, Michael Kundi, Inna Tulaeva, Daria Fomina, Alexander Karaulov, Rudolf Valenta","doi":"10.1111/all.16542","DOIUrl":"https://doi.org/10.1111/all.16542","url":null,"abstract":"<p><strong>Background: </strong>Molecular forms of allergen-specific immunotherapy (AIT) for cat allergy are needed. Fel d 1, Fel d 4, and Fel d 7 are the most important cat allergens.</p><p><strong>Methods: </strong>IgE epitopes of Fel d 4 and Fel d 7 were mapped by blocking allergic patients' IgE binding with allergen peptide-specific antisera. Five recombinant fusion proteins (PreS-Cat 1-PreS-Cat 5) each containing hepatitis B virus (HBV)-derived PreS as an immunological carrier and non-allergenic peptides from the IgE binding sites of Fel d 1, Fel d 4, and Fel d 7 were expressed in Escherichia coli, purified, and characterized by mass spectrometry, circular dichroism (CD), and size exclusion chromatography. ImmunoCAP and basophil activation experiments demonstrated the hypoallergenic activity of PreS-Cat 1-5. The ability of PreS-Cat 1-5 to induce IgE-blocking antibodies in rabbits was compared to three licensed allergen extract-based AIT vaccines. PreS-Cat 1-5-specific IgG antibodies were tested for inhibition of allergen-specific IgE binding and specific basophil activation. T cell activation and induction of specific cytokine secretion by PreS-Cat proteins were compared with cat allergens in PBMC cultures.</p><p><strong>Results: </strong>Recombinant hypoallergenic, biochemically and structurally defined PreS-Cat 1-5 were obtained. Two subcutaneous immunizations of rabbits with PreS-Cat 1-5 induced equal (Fel d 1) or better (Fel d 4 and Fel d 7) antibodies (PreS-Cat 5 > PreS-Cat 1 > PreS-Cat 3) blocking allergic patients' IgE binding to cat allergens than six to fifteen immunizations with allergen extract-based vaccines. PreS-Cat-specific antibodies strongly inhibited specific basophil activation. PreS-Cat 5 > PreS-Cat 1 induced significantly more IL-10 in cultured PBMCs from cat allergic patients than cat allergens.</p><p><strong>Conclusions: </strong>PreS-Cat 5 and PreS-Cat 1 are highly promising molecular vaccine candidates for AIT of cat allergy, combining Fel d 1-, Fel d 4-, and Fel d 7-peptides in single PreS fusion proteins.</p>","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Adventitial Stromal Cells and Myofibroblasts Recruit Pro- and Anti-Inflammatory Immune Cells in Allergic Airway Inflammation\".","authors":"","doi":"10.1111/all.16549","DOIUrl":"https://doi.org/10.1111/all.16549","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NLRC4 Regulates Th2 Differentiation in Mice With Allergic Airway Inflammation Induced by House Dust Mite.","authors":"So-Won Pak, Woong-Il Kim, Se-Jin Lee, Junhyeong Lee, Min-Jung Park, Jong-Hwan Park, Jong-Choon Kim, Taesoo Kim, Joong-Sun Kim, Yun Hee Kim, In-Sik Shin","doi":"10.1111/all.16550","DOIUrl":"https://doi.org/10.1111/all.16550","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOP10-SCAR: A Global Pharmacovigilance Study on Medications Most Frequently Related to Severe Cutaneous Adverse Reactions.","authors":"Jaehyeong Cho, Hyesu Jo, Jaeyu Park, Jeongseon Oh, Tae Hyeon Kim, Kyeongmin Lee, Hayeon Lee, Jinyoung Jeong, Sooji Lee, Michael Miligkos, Nikolaos G Papadopoulos, Dong Keon Yon","doi":"10.1111/all.16544","DOIUrl":"https://doi.org/10.1111/all.16544","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":" ","pages":""},"PeriodicalIF":12.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiology of Chronic Urticaria in Cape Town, South Africa: A Review of Two Tertiary Referral Centers","authors":"Cascia Day, Mimi Deetlefs, Lovemore Mapahla, Yejin Jang, Qiqa Gusha‐Mhlekude, Sicelo Ntuli, Balican Hlungwani, Susanna Kannenberg, Emma Kruger, Ndapewa Ambondo, Willem Visser, Thuraya Isaacs, Rannakoe Lehloenya, Jonny Peter","doi":"10.1111/all.16547","DOIUrl":"https://doi.org/10.1111/all.16547","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"12 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical Distribution and Quantification of IgE Secretion in Mouse Models of House Dust Mite Allergy","authors":"Paul Haase, Angga Perima, Venkat Raman Ramnarayan, Patrick England, Bruno Iannascoli, Andre Limnander, Seblewongel Asrat, Andrea Vecchione, Jamie M. Orengo, Matthew A. Sleeman, Pierre Bruhns","doi":"10.1111/all.16546","DOIUrl":"https://doi.org/10.1111/all.16546","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"4 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta‐Analysis of PQ Grass 27600 SU Efficacy and Quality of Life From Phase III Trials","authors":"Stefan Zielen, Jonathan A. Bernstein, Oliver Pfaar, Lawrence DuBuske, Ralph Mösges, Sven Becker, Fiona Sellwood, Matthias F. Kramer, Murray Skinner, Pieter‐Jan de Kam","doi":"10.1111/all.16535","DOIUrl":"https://doi.org/10.1111/all.16535","url":null,"abstract":"","PeriodicalId":122,"journal":{"name":"Allergy","volume":"2 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological In Vitro Assay for Quantification of Adjuvanted Allergoids","authors":"S. Schlünder, J. Echternach, D. Bartel, V. Mahler, M. D. Mühlebach, F. Führer","doi":"10.1111/all.16543","DOIUrl":"https://doi.org/10.1111/all.16543","url":null,"abstract":"BackgroundMany IgE‐mediated allergic disorders can be treated with allergen immunotherapy (AIT). In order to improve safety and efficacy, some AIT products contain allergen extracts which are chemically cross‐linked to generate allergoids and are adsorbed to aluminum hydroxide adjuvant. The modification and adsorption impair accessibility of the protein and quantification of the allergoid content.MethodsAn ELISA‐like assay to quantify the allergoid content in adjuvanted grass pollen allergoid AIT products (from here on called: AIT drug products; AIT‐DPs) was developed using a fluorescence detection system. The high density of the aluminum hydroxide particles enabled pelleting the antigen complexes by centrifugation. Rabbit anti‐grass pollen allergoid sera or a mouse anti‐Phl p 5 monoclonal antibody (mAb) was used as the primary antibody. Protein content of the samples was quantified by nitrogen analysis.ResultsHigh specificity of the primary antibodies was confirmed by isoelectric focusing, gel‐electrophoresis, and immunoblotting. Performance of the allergoid content assay was demonstrated in grass pollen AIT‐DPs with high specificity and low/absent cross‐reactivity with tree pollen or mite AIT‐DPs. It was used to confirm batch‐to‐batch consistency and allergoid content of distinct grass pollen AIT‐DPs. Overall, in relation to their total protein content, the allergoid content ranged between 0.8 and 2.1 relative to an in‐house reference for all grass pollen AIT‐DPs, whereas use of mAb revealed product‐specific differences in the Phl p 5 amount. Additionally, the assay detects product alteration by heat stress.ConclusionThe described assay is suitable to quantify the allergoid content and quality of allergoids in complex with aluminum hydroxide. It is suitable for animal‐free final product testing in vitro, for example, for batch release to ensure the quality of AIT‐DPs.","PeriodicalId":122,"journal":{"name":"Allergy","volume":"82 1","pages":""},"PeriodicalIF":12.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}