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Molecular survey of certain protozoan agents that cause diarrhea in children in Sudan. 对引起苏丹儿童腹泻的某些原生动物病原体进行分子调查。
F1000Research Pub Date : 2024-10-23 eCollection Date: 2022-01-01 DOI: 10.12688/f1000research.123652.3
Mosab Adam, Hongwei Shen, Khalid-A Enan, Hao Wang, Azza B Musa Musa, Abdel R El Hussein, Isam M Khidir, Xuejun Ma
{"title":"Molecular survey of certain protozoan agents that cause diarrhea in children in Sudan.","authors":"Mosab Adam, Hongwei Shen, Khalid-A Enan, Hao Wang, Azza B Musa Musa, Abdel R El Hussein, Isam M Khidir, Xuejun Ma","doi":"10.12688/f1000research.123652.3","DOIUrl":"10.12688/f1000research.123652.3","url":null,"abstract":"<p><strong>Introduction: </strong>Diarrhea is a significant health problem in the Third World. Identification of the pathogen that causes diarrhea is vital for measures to prevent and control this disease. There are also very few reports of diarrhea in Sudan. Our study aimed to determine the Prevalence of specific protozoan pathogens ( <i>Entamoeba histolytica</i>, <i>Cryptosporidium parvum.</i>, and <i>Giardia spp</i>) in children in Khartoum, Sudan.</p><p><strong>Methods: </strong>We conducted a cross-sectional survey among children under five years of age hospitalized with acute diarrhea between April and December 2014. Diarrheal stool samples were collected, and <i>E. histolytica</i>, <i>C. parvum</i>, and <i>Giardia spp</i> were examined using multiplex real-time PCR.</p><p><strong>Results: </strong>Four hundred and thirty-seven children with acute diarrhea were included in this study; the higher prevalence of diarrhea was in the age ≤ 2 years old (403, 92.2%), >2-≤4 years (32, 7.3%), and >4-<5 years (2, 0.5%). The male-to-female ratio in this study was 1:1.7. Infection with intestinal parasite was found in 155 (35.5%) cases, and co-infection was detected in 16 (3.7%) cases. <i>Giardia spp</i> (18.8%) and <i>C. parvum</i> (15.8%) were the most frequently identified parasites, followed by <i>E. histolytica</i> (0.9). The parasite infection rate was highest and lowest in the under 2-year-old group 143 (35.5%) and the 2-4-year-old group 12 (37.5%). The infection rate was higher in boys 104 (37.7%) than in girls 51 (31.7%). The number of positive cases was higher in the rainy season (August to December) 143 (37.4%), corresponding with that in the dry Season (April to June) 12 (21.8%).</p><p><strong>Discussion: </strong>Our present study demonstrated the high prevalence of <i>Giardia spp</i> and <i>C. parvum</i> in children with diarrhea in the Khartoum region and the usefulness of the multiplex real-time method in disclosing pathogenic protozoal agents. Our result highlighted the necessity of developing intervention measurement and control strategies to deal with childhood parasitic diarrhea in this region.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"11 ","pages":"1401"},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11325136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Length-weight relationship and condition factor of Nile tilapia ( Oreochromis niloticus) fed diets supplemented with guava and star gooseberry leaf extract. 尼罗罗非鱼(Oreochromis niloticus)饲喂添加番石榴和鹅掌楸叶提取物的日粮的体长-体重关系和体况系数。
F1000Research Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.145369.1
Manoj Tukaram Kamble, Krishna Rugmini Salin, Balasaheb Ramdas Chavan, Seema Vijay Medhe, Kim D Thompson, Nopadon Pirarat
{"title":"Length-weight relationship and condition factor of Nile tilapia ( <i>Oreochromis niloticus</i>) fed diets supplemented with guava and star gooseberry leaf extract.","authors":"Manoj Tukaram Kamble, Krishna Rugmini Salin, Balasaheb Ramdas Chavan, Seema Vijay Medhe, Kim D Thompson, Nopadon Pirarat","doi":"10.12688/f1000research.145369.1","DOIUrl":"10.12688/f1000research.145369.1","url":null,"abstract":"<p><strong>Background: </strong>Nile tilapia ( <i>Oreochromis niloticus</i>) is predominant cultured species in aquaculture. However, there is a scarcity of literature regarding relationship between guava and star gooseberry leaf extract and the condition factor. Thus, the present study aims to investigate the effect of guava and star gooseberry leaf extract-supplemented diets on the specific growth rate, length-weight relationship, and condition factor of Nile tilapia.</p><p><strong>Methods: </strong>Six hundred and thirty Nile tilapia (8.7±0.4 g) were randomly distributed among twenty-one tanks (30 fish per tank) within a recirculation system. Over a 60-day period, the fish were fed diets supplemented with 5g/Kg and 10g/Kg of guava leaf extract (GLE-5, GLE-10), star gooseberry leaf extract (SGLE-5, SGLE-10), and a mixture of both (MxLE-5, MXLE-10). Subsequently, specific growth rate, length-weight relationship, and condition factor were determined.</p><p><strong>Results: </strong>After 60 days, the specific growth rate was significantly higher in all the GLE, SGLE, and MxLE groups compared to the control group. The final lengths and weights differed significantly both in the control group and all the GLE, SGLE, and MxLE groups. The analysis of the regression equation indicated a positive correlation (r = 0.970, 0.977, 0.976, 0.974, 0.974, 0.974, and 0.969) between the length and weight of Nile tilapia in the control group and in all the GLE, SGLE, and MxLE groups. The regression exponent \" <i>b</i>\" values in all the GLE, SGLE, and MxLE groups were >3, indicating a positive allometric growth pattern in Nile tilapia compared to the control ( <i>b</i>=2.866), which exhibited a negative allometry. The final condition factor values did not differ significantly in either the control group or any of the plant extract groups.</p><p><strong>Conclusions: </strong>Nile tilapia exhibited positive allometric growth patterns and maintained good health when fed with GLE, SGLE, and MxLE groups. Therefore, these plant extracts demonstrate suitability for commercial Nile tilapia production.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"540"},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TOP2DFVT: An Efficient Matlab Implementation for Topology Optimization based on the Finite-Volume Theory. TOP2DFVT:基于有限体积理论的拓扑优化的高效 Matlab 实现。
F1000Research Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.150945.2
Marcelo Araujo, Arnaldo Santos Júnior, Romildo Escarpini Filho, Eduardo Lages, Marcio Cavalcante
{"title":"TOP2DFVT: An Efficient Matlab Implementation for Topology Optimization based on the Finite-Volume Theory.","authors":"Marcelo Araujo, Arnaldo Santos Júnior, Romildo Escarpini Filho, Eduardo Lages, Marcio Cavalcante","doi":"10.12688/f1000research.150945.2","DOIUrl":"https://doi.org/10.12688/f1000research.150945.2","url":null,"abstract":"<p><p>The finite-volume theory has shown to be numerically efficient and stable for topology optimization of continuum elastic structures. The significant features of this numerical technique are the local satisfaction of equilibrium equations and the employment of compatibility conditions along edges in a surface-averaged sense. These are essential properties to adequately mitigate some numerical instabilities in the gradient version of topology optimization algorithms, such as checkerboard, mesh dependence, and local minima issues. Several computational tools have been proposed for topology optimization employing analysis domains discretized with essential features for finite-element approaches. However, this is the first contribution to offer a platform to generate optimized topologies by employing a Matlab code based on the finite-volume theory for compliance minimization problems. The Top2DFVT provides a platform to perform 2D topology optimization of structures in Matlab, from domain initialization for structured meshes to data post-processing. This contribution represents a significant advancement over earlier publications on topology optimization based on the finite-volume theory, which needed more efficient computational tools. Moreover, the Top2DFVT algorithm incorporates SIMP and RAMP material interpolation schemes alongside sensitivity and density filtering techniques, culminating in a notably enhanced optimization tool. The application of this algorithm to various illustrative cases confirms its efficacy and underscores its potential for advancing the field of structural optimization.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"805"},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The development of a roadmap for Health Technology Assessment implementation in Moldova. 制定摩尔多瓦卫生技术评估实施路线图。
F1000Research Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.146025.2
Gavin Surgey, Ghenadie Curocichin, Maria Garabajiu, Tanja Novakovic, Adrian Pana, Wija Oortwijn
{"title":"The development of a roadmap for Health Technology Assessment implementation in Moldova.","authors":"Gavin Surgey, Ghenadie Curocichin, Maria Garabajiu, Tanja Novakovic, Adrian Pana, Wija Oortwijn","doi":"10.12688/f1000research.146025.2","DOIUrl":"10.12688/f1000research.146025.2","url":null,"abstract":"<p><strong>Background: </strong>Moldova strives for universal health coverage (UHC) and considers health technology assessment (HTA) an important policy instrument to inform the choice of services that should be covered to progressively realize UHC. It plays a key role in determining which technologies are coved, considering various dimensions often including cost-effectiveness, budget impact, and feasibility. This paper reports on work undertaken to develop a roadmap for HTA implementation, using evidence-informed deliberative processes (EDPs) as the guiding framework.</p><p><strong>Methods: </strong>Between 2020 and 2022, we undertook several activities that informed the roadmap. We conducted a needs assessment and an assessment of European HTA best practices using a combination of desk research, interviews and surveys. We then conducted a document review of six selected HTA systems, complimented by expert interviews from three neighbouring countries.</p><p><strong>Results: </strong>The roadmap provided a suggested HTA process, which included instructions on how to execute the different steps of the EDP framework to enhance the legitimacy of HTA-informed decision-making. The roadmap encompasses several aspects such as how to organize stakeholder involvement, how to identify and operationalize decision criteria, and how to make the decision process transparent. Guidance was given to the country on establishing a legal framework for HTA; a communication strategy; strengthening capacity and leveraging HTA expertise. The country was also given tailored advice on the positioning of the HTA-agency, first as an entity within the Ministry of Health or the National Agency of Public Health but with the long-term vision for it to be independent of any state institution.</p><p><strong>Conclusions: </strong>Despite several challenges, including a partial disruption because of the Covid-19 pandemic, the development of the roadmap for HTA implementation was completed and agreed upon by the Ministry of Health in Moldova. This work has helped motivate and support the country in HTA institutionalization.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"518"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Case Report: Placental site trophoblastic tumor revealed by a clinical pelvic abscess. 病例报告:临床盆腔脓肿揭示的胎盘部位滋养细胞肿瘤
F1000Research Pub Date : 2024-10-21 eCollection Date: 2022-01-01 DOI: 10.12688/f1000research.75075.3
Souayeh Nesrine, Hajer Bettaieb, Wael Mbarki, Ben Brahim Ehsen, Helal Imen, Ben Nasr Mehdi, Oueslati Hedhili, Hsayaoui Najeh, Chaouki Mbarki
{"title":"Case Report: Placental site trophoblastic tumor revealed by a clinical pelvic abscess.","authors":"Souayeh Nesrine, Hajer Bettaieb, Wael Mbarki, Ben Brahim Ehsen, Helal Imen, Ben Nasr Mehdi, Oueslati Hedhili, Hsayaoui Najeh, Chaouki Mbarki","doi":"10.12688/f1000research.75075.3","DOIUrl":"https://doi.org/10.12688/f1000research.75075.3","url":null,"abstract":"<p><p>We report an uncommon clinical presentation of a placental site trophoblastic tumor. The patient presented initially with abdominal pain with, fever, bleeding and pelvic mass on ultrasonography leading to the wrong diagnosis of a pelvic abscess. Dilation and curettage were performed and pathological examination confirmed the diagnosis of placental site trophoblastic tumor. Therefore, she underwent abdominal hysterectomy. Four years after surgery, the patient is still disease free. Gestational trophoblastic diseases should be considered in every patient presenting abnormal uterine bleeding after delivery or pregnancy loss despite the associated symptoms being very unusual.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"11 ","pages":"129"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical rationale for dietary lutein supplementation in long COVID and mRNA vaccine injury syndromes. 在长 COVID 和 mRNA 疫苗损伤综合征中补充叶黄素的临床依据。
F1000Research Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.143517.3
Anthony M Kyriakopoulos, Greg Nigh, Peter A McCullough, Stephanie Seneff
{"title":"Clinical rationale for dietary lutein supplementation in long COVID and mRNA vaccine injury syndromes.","authors":"Anthony M Kyriakopoulos, Greg Nigh, Peter A McCullough, Stephanie Seneff","doi":"10.12688/f1000research.143517.3","DOIUrl":"https://doi.org/10.12688/f1000research.143517.3","url":null,"abstract":"<p><p>Lutein, a plant-derived xanthophyl-carotenoid, is an exceptional antioxidant and anti-inflammatory constituent found in food. High dietary intake of lutein is beneficial against eye disease, improves cardiometabolic health, protects from neurodegenerative diseases, and is beneficial for liver, kidney, and respiratory health. Lutein protects against oxidative and nitrosative stress, both of which play a major role in long COVID and mRNA vaccination injury syndromes. Lutein is an important natural agent for therapeutic use against oxidative and nitrosative stress in chronic illnesses such as cardiovascular and neurodegenerative diseases and cancer. It can also potentially inhibit spike protein-induced inflammation. Rich dietary supplementation of lutein, naturally derived in non-biodegradable Extra Virgin Olive Oil (EVOO), can most optimally be used against oxidative and nitrosative stress during post-COVID and mRNA vaccination injury syndromes. Due to its high oleic acid (OA) content, EVOO supports optimal absorption of dietary lutein. The main molecular pathways by which the SARS-CoV-2 spike protein induces pathology, nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) and activated protein (AP)-1, can be suppressed by lutein. Synergy with other natural compounds for spike protein detoxification is likely.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"191"},"PeriodicalIF":0.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real Customization or Just Marketing: Are Customized Versions of Generative AI Useful? 真正的定制还是纯粹的营销?生成式人工智能的定制版本有用吗?
F1000Research Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.153129.2
Eduardo C Garrido-Merchán, Jose Luis Arroyo-Barrigüete, Francisco Borrás-Pala, Leandro Escobar-Torres, Carlos Martínez de Ibarreta, Jose María Ortíz-Lozano, Antonio Rua-Vieites
{"title":"Real Customization or Just Marketing: Are Customized Versions of Generative AI Useful?","authors":"Eduardo C Garrido-Merchán, Jose Luis Arroyo-Barrigüete, Francisco Borrás-Pala, Leandro Escobar-Torres, Carlos Martínez de Ibarreta, Jose María Ortíz-Lozano, Antonio Rua-Vieites","doi":"10.12688/f1000research.153129.2","DOIUrl":"10.12688/f1000research.153129.2","url":null,"abstract":"<p><strong>Background: </strong>Large Language Models (LLMs), as in the case of OpenAI <sup>TM</sup> ChatGPT-4 <sup>TM</sup> Turbo, are revolutionizing several industries, including higher education. In this context, LLMs can be personalised through customization process to meet the student demands on every particular subject, like statistics. Recently, OpenAI launched the possibility of customizing their model with a natural language web interface, enabling the creation of customised GPT versions deliberately conditioned to meet the demands of a specific task.</p><p><strong>Methods: </strong>This preliminary research aims to assess the potential of the customised GPTs. After developing a Business Statistics Virtual Professor (BSVP), tailored for students at the Universidad Pontificia Comillas, its behaviour was evaluated and compared with that of ChatGPT-4 Turbo. Firstly, each professor collected 15-30 genuine student questions from \"Statistics and Probability\" and \"Business Statistics\" courses across seven degrees, primarily from second-year courses. These questions, often ambiguous and imprecise, were posed to ChatGPT-4 Turbo and BSVP, with their initial responses recorded without follow-ups. In the third stage, professors blindly evaluated the responses on a 0-10 scale, considering quality, depth, and personalization. Finally, a statistical comparison of the systems' performance was conducted.</p><p><strong>Results: </strong>The results lead to several conclusions. Firstly, a substantial modification in the style of communication was observed. Following the instructions it was trained with, BSVP responded in a more relatable and friendly tone, even incorporating a few minor jokes. Secondly, when explicitly asked for something like, \"I would like to practice a programming exercise similar to those in R practice 4,\" BSVP could provide a far superior response. Lastly, regarding overall performance, quality, depth, and alignment with the specific content of the course, no statistically significant differences were observed in the responses between BSVP and ChatGPT-4 Turbo.</p><p><strong>Conclusions: </strong>It appears that customised assistants trained with prompts present advantages as virtual aids for students, yet they do not constitute a substantial improvement over ChatGPT-4 Turbo.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"791"},"PeriodicalIF":0.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The first report on detecting SARS-CoV-2 inside bacteria of  the human gut microbiome: A case series on asymptomatic family members and a child with COVID-19. 首次在人体肠道微生物群细菌中检测到SARS-CoV-2:无症状家庭成员和COVID-19儿童病例系列报道
F1000Research Pub Date : 2024-10-16 eCollection Date: 2022-01-01 DOI: 10.12688/f1000research.77421.2
Carlo Brogna, Simone Cristoni, Mauro Petrillo, Domenico Rocco Bisaccia, Francesco Lauritano, Luigi Montano, Marina Prisco, Marina Piscopo
{"title":"The first report on detecting SARS-CoV-2 inside bacteria of  the human gut microbiome: A case series on asymptomatic family members and a child with COVID-19.","authors":"Carlo Brogna, Simone Cristoni, Mauro Petrillo, Domenico Rocco Bisaccia, Francesco Lauritano, Luigi Montano, Marina Prisco, Marina Piscopo","doi":"10.12688/f1000research.77421.2","DOIUrl":"10.12688/f1000research.77421.2","url":null,"abstract":"<p><p>Many studies report the importance of using feces as source sample for detecting SARS-CoV-2 in patients with COVID-19 symptoms but who are negative to oropharyngeal/ nasopharyngeal tests. Here, we report the case of an asymptomatic child whose family members had negative results with the rapid antigen nasopharyngeal swab tests. The 21-month-old child presented with fever, diarrhea, bilateral conjunctivitis, and conspicuous lacrimation. In this study, analysis for the presence of SARS-CoV-2 in fecal samples by using Luminex technology allowed accurate detection of the presence of the viral RNA in the feces of the child and of all her relatives, which thus resulted to be positive but asymptomatic. It is the first time that SARS-CoV-2- is observed inside the bacteria of the human gut microbiome and outside a matrix resembling extracellular bacterial lysates, in agreement with a bacteriophage mechanism with the images obtained by transmission electron microscopy (TEM), post-embedding immunogold, and by fluorescence microscope. In addition to the typical observations of respiratory symptoms, accurate evaluation of clinical gastrointestinal and neurological symptoms, combined with efficient highly sensitive molecular testing on feces, represent an efficient approach for detecting SARS-CoV-2, and for providing the correct therapy in challenging COVID-19 cases, like the one here reported.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":" ","pages":"135"},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43595958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A validated and standardized pseudotyped microneutralization assay as a safe and powerful tool to measure LASSA virus neutralising antibodies for vaccine development and comparison. 经过验证和标准化的假定型微中和试验,作为测量 LASSA 病毒中和抗体的安全而强大的工具,用于疫苗开发和比较。
F1000Research Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.12688/f1000research.149578.2
Roberta Antonelli, Vittoria Forconi, Eleonora Molesti, Claudia Semplici, Pietro Piu, Maria Altamura, Francesca Dapporto, Nigel Temperton, Emanuele Montomoli, Alessandro Manenti
{"title":"A validated and standardized pseudotyped microneutralization assay as a safe and powerful tool to measure LASSA virus neutralising antibodies for vaccine development and comparison.","authors":"Roberta Antonelli, Vittoria Forconi, Eleonora Molesti, Claudia Semplici, Pietro Piu, Maria Altamura, Francesca Dapporto, Nigel Temperton, Emanuele Montomoli, Alessandro Manenti","doi":"10.12688/f1000research.149578.2","DOIUrl":"https://doi.org/10.12688/f1000research.149578.2","url":null,"abstract":"<p><strong>Background: </strong>Over the past few decades, World Health Organization (WHO) has made massive efforts to promote the development of a vaccine against Lassa virus (LASV), one of the top ten priority pathogens for research and development under the WHO R&D Blueprint for Emerging Infections. To date, several vaccines are at different stages of development. In this scenario, a validated and standardised assay to measure LASV neutralising antibodies is urgently needed for vaccine development and comparison.</p><p><strong>Methods: </strong>The neutralisation assay remains the gold standard for determining antibody efficacy. Here we have proposed a safe and validated pseudotyped neutralisation assay for LASV, taking advantage of the development of the first WHO International Standard and Reference Panel for Anti-Lassa Fever (NIBSC code 21/332).</p><p><strong>Results and conclusions: </strong>The proposed results demonstrate that the pseudotyped luciferase neutralisation assay is a specific serological test for the measurement of LASV neutralising antibodies without cross-reacting with standard sera specific for heterologous viral infections. In addition, the assay is accurate, precise, and linear according to criteria and statistical analyses defined and accepted by international guidelines.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"534"},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Linkages between service recovery system and customer justice perceptions: A multi-level model of employee service recovery performance. 服务恢复系统与客户正义感之间的联系:员工服务恢复绩效的多层次模型。
F1000Research Pub Date : 2024-10-14 eCollection Date: 2023-01-01 DOI: 10.12688/f1000research.135103.2
Abhishek S Rao, Yogesh Pai P, Lakshminarayanan Sethumadhavan
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