EXCLI Journal最新文献_第9页

Recent advances, challenges and updates on the development of therapeutics for malaria. 疟疾治疗药物开发的最新进展、挑战和更新。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-05-06 eCollection Date: 2024-01-01 DOI: 10.17179/excli2023-6856

Rimmy Nandal, Davinder Kumar, Navidha Aggarwal, Virender Kumar, Balasubramanian Narasimhan, Rakesh Kumar Marwaha, Prabodh Chander Sharma, Surender Kumar, Nitin Bansal, Hitesh Chopra, Aakash Deep

{"title":"Recent advances, challenges and updates on the development of therapeutics for malaria.","authors":"Rimmy Nandal, Davinder Kumar, Navidha Aggarwal, Virender Kumar, Balasubramanian Narasimhan, Rakesh Kumar Marwaha, Prabodh Chander Sharma, Surender Kumar, Nitin Bansal, Hitesh Chopra, Aakash Deep","doi":"10.17179/excli2023-6856","DOIUrl":"10.17179/excli2023-6856","url":null,"abstract":"Malaria has developed as a serious worldwide health issue as a result of the introduction of resistant Plasmodium species strains. Because of the common chemo resistance to most of the existing drugs on the market, it poses a severe health problem and significant obstacles in drug research. Malaria treatment has evolved during the last two decades in response to Plasmodium falciparum drug sensitivity and a return of the disease in tropical areas. Plasmodium falciparum is now highly resistant to the majority of antimalarial drugs. The parasite resistance drew focus to developing novel antimalarials to combat parasite resistance. The requirement for many novel antimalarial drugs in the future year necessitates adopting various drug development methodologies. Different innovative strategies for discovering antimalarial drugs are now being examined here. This review is primarily concerned with the description of newly synthesized antimalarial compounds, i.e. Tafenoquine, Cipargamin, Ferroquine, Artefenomel, DSM265, MMV390048 designed to improve the activity of pure antimalarial enantiomers. In this review, we selected the representative malarial drugs in clinical trials, classified them with detailed targets according to their action, discussed the relationship within the human trials, and generated a summative discussion with prospective expectations.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"672-713"},"PeriodicalIF":4.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of microbial transglutaminase by Streptoverticillium cinnamoneum KKP 1658. 肉桂链霉菌（Streptoverticillium cinnamoneum KKP 1658）产生微生物转谷氨酰胺酶。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-05-03 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7033

Vitaliy Kolotylo, Kamil Piwowarek, Marek Kieliszek

引用次数: 0

Exosomes derived from colorectal cancer cells take part in activation of stromal fibroblasts through regulating PHLPP isoforms. 来自结直肠癌细胞的外泌体通过调节 PHLPP 同工酶参与基质成纤维细胞的激活。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-05-02 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-6926

Fatemeh Khaloozadeh, Ehsan Razmara, Fatemeh Asgharpour-Babayian, Alireza Fallah, Reihaneh Ramezani, Fatemeh Rouhollah, Sadegh Babashah

{"title":"Exosomes derived from colorectal cancer cells take part in activation of stromal fibroblasts through regulating PHLPP isoforms.","authors":"Fatemeh Khaloozadeh, Ehsan Razmara, Fatemeh Asgharpour-Babayian, Alireza Fallah, Reihaneh Ramezani, Fatemeh Rouhollah, Sadegh Babashah","doi":"10.17179/excli2024-6926","DOIUrl":"10.17179/excli2024-6926","url":null,"abstract":"Given that tumor cells primarily instigate systemic changes through exosome secretion, our study delved into the role of colorectal cancer (CRC)-secreted exosomal miR-224 in stromal reprogramming and its impact on endothelial cell angiogenesis. Furthermore, we assessed the potential clinical significance of a specific signature of circulating serum-derived miRNAs, serving as a non-invasive biomarker for CRC diagnosis. Circulating serum-derived miR-103a-3p, miR-135b-5p, miR-182-5p, and miR-224-5p were significantly up-regulated, while miR-215-5p, and miR-455-5p showed a significant down-regulation in CRC patients than in healthy individuals. Our findings indicated that the expressions of CAF-specific markers (α-SMA and FAP) and CAF-derived cytokines (IL-6, and SDF-1) were induced in fibroblasts stimulated with SW480 CRC exosomes, partly due to Akt activation. As a plausible mechanism, exosomal transfer of miR-224 from SW40 CRC cells may activate stromal fibroblasts, which in turn, may promote endothelial cell sprouting. The study identified PHLPP1 and PHLPP2 as direct targets of miR-224 and demonstrated that CRC-secreted exosomal miR-224 activates Akt signaling by regulating PHLPP1/2 in activated fibroblasts, thereby affecting the stromal cell proliferation and migration. This study established a panel of six-circulating serum-derived miRNAs as a non-invasive biomarker for CRC diagnosis. Also, we proposed a supporting model in which CRC-secreted exosomal miR-224 takes part in the stromal reprogramming to CAFs partly through regulating Akt signaling. This may affect the malignant biological behavior of activated stromal cells and thereby elicit a vascular response within the microenvironment of CRC cells. See also the graphical abstract(Fig. 1).","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"634-654"},"PeriodicalIF":4.6,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital blindness: the hidden cost of excessive screen time. 数字盲症：屏幕时间过长的隐性代价。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7041

Nikhil Aggarwal, Noor Us Saba

引用次数: 0

The influence of gut microbiota on the progression of Type 2 Diabetes: a new perspective for treatment and prevention. 肠道微生物群对 2 型糖尿病进展的影响：治疗和预防的新视角。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7036

Lysandro P Borges, Pamela C de Jesus, Jessiane B de Souza, Deise M R R Silva, Pedro H M Moura, Ronaldy S Santos, Marina Dos S Barreto, Adriana G Guimarães, Lucas A da M Santana, Otavio Cabral-Marques, Eloia E D Silva

引用次数: 0

The offspring sex ratio at birth in one of the largest human harems. 人类最大后宫之一的后代出生性别比。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-29 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7020

Mostafa Saadat

引用次数: 0

Paraffin embedding of the whole human cerebral hemisphere to assess arterial distribution territories. 对整个人类大脑半球进行石蜡包埋，以评估动脉分布区域。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-29 eCollection Date: 2024-01-01 DOI: 10.17179/excli2023-6601

Mykyta Smirnov, Frédéric Andersson, Laurent Barantin, Igor Lima Maldonado, Christophe Destrieux

{"title":"Paraffin embedding of the whole human cerebral hemisphere to assess arterial distribution territories.","authors":"Mykyta Smirnov, Frédéric Andersson, Laurent Barantin, Igor Lima Maldonado, Christophe Destrieux","doi":"10.17179/excli2023-6601","DOIUrl":"10.17179/excli2023-6601","url":null,"abstract":"Commonly used to decode the human brain's structural complexity, ex vivo dissection focuses on a given structure or region but cannot depict the whole brain organization (for example, its arterial distribution territories). Where dissection reaches its limit, the combination of tissue sectioning and 3D reconstruction may provide a volume for the assessment of structures from any view angle, following them dynamically to understand their spatial relationships. However, to produce sections, standard histological tissue processing protocols for paraffin embedding cannot be applied to a cerebral hemisphere as the latter is extensively larger than the conventional specimens. This paper presents a protocol for paraffin embedding of the whole human cerebral hemisphere and a method to reconstruct 3D volumes from serially sectioned and photographed paraffin blocks containing embedded hemispheres. Seven ex vivo whole human cerebral hemispheres were included, two were serially sectioned. Main cerebral arteries were injected with colored media to label arterial territories. A detailed description of every step, from tissue processing to image acquisition of cut blockfaces and volume reconstruction, is provided. Tissue processing and section cutting were reproducible, and the former provided complete and homogeneous paraffin wax impregnation. 3D visualization of the reconstructed whole human cerebral hemisphere successfully showed the distribution territories of the main cerebral arteries. In addition, we discuss the challenges we faced and overcame while developing the presented method and highlight its originality.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"612-623"},"PeriodicalIF":4.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferroptosis and circular RNAs: new horizons in cancer therapy. 铁突变和环状 RNA：癌症治疗的新视野。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-25 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7005

Asif Ahmad Bhat, Neelima Kukreti, Muhammad Afzal, Ahsas Goyal, Riya Thapa, Haider Ali, Moyad Shahwan, Waleed Hassan Almalki, Imran Kazmi, Sami I Alzarea, Sachin Kumar Singh, Kamal Dua, Gaurav Gupta

{"title":"Ferroptosis and circular RNAs: new horizons in cancer therapy.","authors":"Asif Ahmad Bhat, Neelima Kukreti, Muhammad Afzal, Ahsas Goyal, Riya Thapa, Haider Ali, Moyad Shahwan, Waleed Hassan Almalki, Imran Kazmi, Sami I Alzarea, Sachin Kumar Singh, Kamal Dua, Gaurav Gupta","doi":"10.17179/excli2024-7005","DOIUrl":"10.17179/excli2024-7005","url":null,"abstract":"Cancer poses intricate challenges to treatment due to its complexity and diversity. Ferroptosis and circular RNAs (circRNAs) are emerging as innovative therapeutic avenues amid the evolving landscape of cancer therapy. Extensive investigations into circRNAs reveal their diverse roles, ranging from molecular regulators to pivotal influencers of ferroptosis in cancer cell lines. The results underscore the significance of circRNAs in modulating molecular pathways that impact crucial aspects of cancer development, including cell survival, proliferation, and metastasis. A detailed analysis delineates these pathways, shedding light on the molecular mechanisms through which circRNAs influence ferroptosis. Building upon recent experimental findings, the study evaluates the therapeutic potential of targeting circRNAs to induce ferroptosis. By identifying specific circRNAs associated with the etiology of cancer, this analysis paves the way for the development of targeted therapeutics that exploit vulnerabilities in cancer cells. This review consolidates the existing understanding of ferroptosis and circRNAs, emphasizing their role in cancer therapy and providing impetus for ongoing research in this dynamic field. See also the graphical abstract(Fig. 1).","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"570-599"},"PeriodicalIF":4.6,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The therapeutic potential of angiotensin-converting enzyme inhibitor enalapril to ameliorate muscle atrophy in a murine model. 血管紧张素转换酶抑制剂依那普利改善小鼠模型肌肉萎缩的治疗潜力。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-25 eCollection Date: 2024-01-01 DOI: 10.17179/excli2023-6822

Sima Seifi, Seyedeh Elnaz Nazari, Amir Avan, Nima Khalili-Tanha, Fereshteh Asgharzadeh, Fatemeh Babaei, Ghazaleh Khalili-Tanha, Seyyedeh Zahra Asghari, Mahdieh Darroudi, Gordon A Ferns, Abdoljalal Marjani, Majid Khazaei

{"title":"The therapeutic potential of angiotensin-converting enzyme inhibitor enalapril to ameliorate muscle atrophy in a murine model.","authors":"Sima Seifi, Seyedeh Elnaz Nazari, Amir Avan, Nima Khalili-Tanha, Fereshteh Asgharzadeh, Fatemeh Babaei, Ghazaleh Khalili-Tanha, Seyyedeh Zahra Asghari, Mahdieh Darroudi, Gordon A Ferns, Abdoljalal Marjani, Majid Khazaei","doi":"10.17179/excli2023-6822","DOIUrl":"10.17179/excli2023-6822","url":null,"abstract":"Muscle atrophy due to limb immobilization and inactivity is a common consequence of many diseases and treatment processes. One of the systems activated in inflammatory conditions is the renin-angiotensin system (RAS). The present study was conducted with the aim of investigating the effects of one of the angiotensin-converting enzyme (ACE) inhibitors, enalapril, on improving muscle atrophy caused by immobility. The study was conducted in three groups: a control, an atrophy, and an atrophy group treated with enalapril on Balb/c mice. After tying a splint to cause atrophy in one of the legs, daily treatment with enalapril intraperitoneally (dissolved in DMSO) at a dose of 10 mg/kg/day was done for 7 days. On the eighth day, the splint was opened and half of the mice were evaluated. Then, in the recovery phase, treatment with enalapril was continued in the remaining mice for 10 days without a splint. At the end of each phase, the mice were examined for the muscle strength of the lower limb muscles, and histological and biochemical analyses were subsequently carried out. The tissue level of the oxidative stress index MDA was evaluated, which showed a significantly lower level in the enalapril group compared to the atrophy group (*P<0.1). Also, inflammatory factors in the enalapril group showed a decrease compared to the atrophy group. The strength of four limbs in the mice of the treatment group (-18.36 ± 1.70 %) was significantly higher than that of the atrophy group (-30.33 ± 3 %) at the end of the atrophy phase and also after 10 days of recovery. The results suggest that the use of enalapril that reduces the activation of angiotensin II-dependent pro-oxidant and pro-inflammatory pathways may improve the functional disorder and muscle necrosis in the murine model of muscle atrophy.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"600-611"},"PeriodicalIF":4.6,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial fatty acid beta-oxidation: a possible therapeutic target for skeletal muscle lipotoxicity in peripheral artery disease myopathy. 线粒体脂肪酸 beta-氧化：外周动脉疾病肌病中骨骼肌脂肪毒性的可能治疗靶点。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2024-04-23 eCollection Date: 2024-01-01 DOI: 10.17179/excli2024-7004

Cassandra E Bradley, Emma Fletcher, Trevor Wilkinson, Andrew Ring, Lucas Ferrer, Dimitrios Miserlis, Pal Pacher, Panagiotis Koutakis

{"title":"Mitochondrial fatty acid beta-oxidation: a possible therapeutic target for skeletal muscle lipotoxicity in peripheral artery disease myopathy.","authors":"Cassandra E Bradley, Emma Fletcher, Trevor Wilkinson, Andrew Ring, Lucas Ferrer, Dimitrios Miserlis, Pal Pacher, Panagiotis Koutakis","doi":"10.17179/excli2024-7004","DOIUrl":"10.17179/excli2024-7004","url":null,"abstract":"Peripheral artery disease (PAD) is an atherosclerotic disease impacting over 200 million individuals and the prevalence increases with age. PAD occurs when plaque builds up within the peripheral arteries, leading to reduced blood flow and oxygen supply to the outer extremities. Individuals who experience PAD suffer from ischemia, which is typically accompanied by significant damage to skeletal muscles. Additionally, this tissue damage affects mitochondria, causing them to become dysregulated and dysfunctional, resulting in decreased metabolic rates. As there is no known cure for PAD, researchers are exploring potential therapeutic targets by examining coexisting cardiovascular conditions and metabolic risk factors, such as the aging process. Among these comorbidities, type-two diabetes mellitus and obesity are particularly common in PAD cases. These conditions, along with aging itself, are associated with an elevated accumulation of ectopic lipids within skeletal muscles, similar to what is observed in PAD. Researchers have attempted to reduce excess lipid accumulation by increasing the rate of fatty acid beta oxidation. Manipulating acetyl coenzyme A carboxylase 2, a key regulatory protein of fatty acid beta oxidation, has been the primary focus of such research. When acetyl coenzyme A carboxylase 2 is inhibited, it interrupts the conversion of acetyl-CoA into malonyl-CoA, resulting in an increase in the rate of fatty acid beta oxidation. By utilizing samples from PAD patients and applying the pharmacological strategies developed for acetyl coenzyme A carboxylase 2 in diabetes and obesity to PAD, a potential new therapeutic avenue may emerge, offering hope for improved quality of life for individuals suffering from PAD.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":"23 ","pages":"523-533"},"PeriodicalIF":4.6,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0