EXCLI Journal最新文献_第8页

Prevalence and impact of tobacco use disorder on in-hospital mortality in patients hospitalized with non-group 1 pulmonary hypertension: a nationwide propensity score-matched analysis, 2019. 非1类肺动脉高压住院患者中烟草使用障碍的患病率及其对院内死亡率的影响：2019年全国倾向得分匹配分析。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-11-28 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6409

Rupak Desai, Zainab Gandhi, Saher Taj Shiza, Akhil Jain, Hiren Koshiya, Bibi Alli-Ramsaroop, Agatha Olawunmi Akinsete, Eko Wilson, Pranathi Rudra, Mohan Sai Sunith Vegesna, Madiha Bari, Ankit Vyas, Bisharah Rizvi, Salim Surani

{"title":"Prevalence and impact of tobacco use disorder on in-hospital mortality in patients hospitalized with non-group 1 pulmonary hypertension: a nationwide propensity score-matched analysis, 2019.","authors":"Rupak Desai, Zainab Gandhi, Saher Taj Shiza, Akhil Jain, Hiren Koshiya, Bibi Alli-Ramsaroop, Agatha Olawunmi Akinsete, Eko Wilson, Pranathi Rudra, Mohan Sai Sunith Vegesna, Madiha Bari, Ankit Vyas, Bisharah Rizvi, Salim Surani","doi":"10.17179/excli2023-6409","DOIUrl":"10.17179/excli2023-6409","url":null,"abstract":"Numerous studies indicated that patients with tobacco use disorder (TUD) are inversely associated with mortality in what is known as the smoker's paradox. However, limited studies have been conducted on the impact of TUD on the in-hospital mortality rates of patients with secondary pulmonary hypertension (PH, Non-Group 1 PH). Using the 2019 National Inpatient Sample, we identified PH and divided it into TUD and non-TUD to compare the comorbidities and in-hospital mortality between the two after 1:1 propensity-score matching. Of 1,129,440 PH hospitalizations, 12.1 % had TUD. After matching (n=133545, each group), TUD had lower median age (62 vs. 63), higher females (49 vs. 46.6 %), blacks (25.9 vs. 25.3 %), lower household income (40.8 vs. 32.7 %), Medicaid (22.4 vs. 14.8 %), non-elective (93.5 vs. 89.8 %), rural (9.3 vs. 6.7 %), urban non-teaching (17.2 vs 15.8 %) admissions. All CV comorbidities and other substance use were higher in TUD except CHF and valvular heart disease, TUD+ cohort and lower mortality (3.3 vs. 4.2 %, OR 0.78, p<0.001), higher routine discharges (53.8 vs. 51.3 %, p<0.001) and lower total charges ($47155 vs. 51909, p<0.001) than non-TUD. Although PH patients with TUD had a higher comorbidity burden, they had lower in-hospital mortality rates along with lower total charges of hospitalization, mandating real-world data to validate these results. See also the Graphical abstract(Fig. 1).","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10776876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro screening of topical formulation excipients for epithelial toxicity in cancerous and non-cancerous cell lines. 体外筛选外用制剂辅料在癌细胞和非癌细胞系中的上皮毒性。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-11-16 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6072

Farzaneh Forouz, Yousuf Mohammed, Hamid S A Shobeiri Nejad, Michael S Roberts, Jeffrey E Grice

{"title":"In vitro screening of topical formulation excipients for epithelial toxicity in cancerous and non-cancerous cell lines.","authors":"Farzaneh Forouz, Yousuf Mohammed, Hamid S A Shobeiri Nejad, Michael S Roberts, Jeffrey E Grice","doi":"10.17179/excli2023-6072","DOIUrl":"10.17179/excli2023-6072","url":null,"abstract":"Chemical excipients used in topical formulations may be toxic to living skin cells. Here, we compared the in vitro toxicity of some common solubilizing excipients against human melanoma cells, human keratinocytes (HaCaT) and primary skin fibroblasts (FB) as examples of cancerous, immortalized and primary human skin cells, often used as experimental models representative of in vivo conditions. Two distinct endpoint assays (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet (CV)) were used. The mechanism of cell death after excipient exposure was assessed through Reactive Oxygen Species (ROS) production, cell membrane integrity and cell cycle progression. Results showed that the surfactants, Labrasol®, Labrafil® and Transcutol®, were less toxic than Triton X-100 (a model irritant) in all cell types whereas the oil, Labrafac®, was non-toxic. The human melanoma WM164 cell line showed the greatest sensitivity toward cytotoxicity after chemical exposure, while the other cell lines were more resistant. The relative excipient cytotoxicity responses observed in the MTT and CV assays were comparable and similar trends were seen in their estimated 50 % inhibitory concentration (IC50) values. DNA fragmentation by flow cytometry after exposing the cells to IC50 concentrations of the excipients showed negligible apoptotic populations. ROS production was increased in all cell types after toxic exposure; however, ROS elevation did not lead to apoptosis. The toxicity profiles of each excipient are not only relevant to their use in formulating safe topical products but also in the potential synergistic efficacy in the topical treatment of melanoma.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10776879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment analysis and chromosomal distribution of gout susceptible loci identified by genome-wide association studies. 全基因组关联研究确定的痛风易感位点的富集分析和染色体分布。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-11-14 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6481

Mostafa Saadat

{"title":"Enrichment analysis and chromosomal distribution of gout susceptible loci identified by genome-wide association studies.","authors":"Mostafa Saadat","doi":"10.17179/excli2023-6481","DOIUrl":"10.17179/excli2023-6481","url":null,"abstract":"Gout is an inherited and common inflammatory arthritic disease. Many researchers will identify polymorphic loci of gout susceptibility by conducting genome-wide association studies (GWAS). In the present study, the enrichment analysis and chromosomal distribution were performed using predicted polymorphic loci associated with gout risk. The polymorphic loci associated to gout were obtained from the GWAS database. Overall, this database contains 64,806 gout patients and 2,856,174 controls. Gene ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed by using the Enrichr online server. A total of 110 common polymorphic protein-coding loci associated with gout risk were identified and included in the analysis. The results of the KEGG analysis showed that the gout-associated loci were mainly related to ABC transporters, endocrine and other factor-regulated calcium reabsorption, and gastric acid secretion pathways. The gene ontology analysis showed that the biological processes of the gout-associated loci were vascular transport, transport across the blood-brain barrier, positive regulation of transporter activity, and positive regulation of transcription by RNA polymerase II. The top cellular component was the external side of the apical plasma membrane. Statistical analysis revealed that the human chromosome segments 1q22, 4p16.1, 6p21.1-p21.2, 11q13.1-q13.2, 12q13.11-q13.3, and 12q24.1 had significantly bearing higher numbers of gout susceptibility loci.","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10776878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and molecular characterization of lopinavir and ivermectin as breast cancer resistance protein (BCRP/ABCG2) inhibitors. 作为乳腺癌抗性蛋白（BCRP/ABCG2）抑制剂的洛匹那韦和依维菌素的结构和分子特征。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-11-14 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6427

Julia de Paula Dutra, Gustavo Scheiffer, Thales Kronenberger, Lucas Julian Cruz Gomes, Isadora Zanzarini, Kelly Karoline Dos Santos, Arun K Tonduru, Antti Poso, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Glaucio Valdameri, Vivian Rotuno Moure

{"title":"Structural and molecular characterization of lopinavir and ivermectin as breast cancer resistance protein (BCRP/ABCG2) inhibitors.","authors":"Julia de Paula Dutra, Gustavo Scheiffer, Thales Kronenberger, Lucas Julian Cruz Gomes, Isadora Zanzarini, Kelly Karoline Dos Santos, Arun K Tonduru, Antti Poso, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Glaucio Valdameri, Vivian Rotuno Moure","doi":"10.17179/excli2023-6427","DOIUrl":"10.17179/excli2023-6427","url":null,"abstract":"A current clinical challenge in cancer is multidrug resistance (MDR) mediated by ABC transporters. Breast cancer resistance protein (BCRP) or ABCG2 transporter is one of the most important ABC transporters implicated in MDR and the use of inhibitors is a promising approach to overcome the resistance in cancer. This study aimed to characterize the molecular mechanism of ABCG2 inhibitors identified by a repurposing drug strategy using antiviral, anti-inflammatory and antiparasitic agents. Lopinavir and ivermectin can be considered as pan-inhibitors of ABC transporters, since both compounds inhibited ABCG2, P-glycoprotein and MRP1. They inhibited ABCG2 activity showing IC50 values of 25.5 and 23.4 µM, respectively. These drugs were highly cytotoxic and not transported by ABCG2. Additionally, these drugs increased the 5D3 antibody binding and did not affect the mRNA and protein expression levels. Cell-based analysis of the type of inhibition suggested a non-competitive inhibition, which was further corroborated by in silico approaches of molecular docking and molecular dynamics simulations. These results showed an overlap of the lopinavir and ivermectin binding sites on ABCG2, mainly interacting with E446 residue. However, the substrate mitoxantrone occupies a different site, binding to the F436 region, closer to the L554/L555 plug. In conclusion, these results revealed the mechanistic basis of lopinavir and ivermectin interaction with ABCG2. See also the Graphical abstract(Fig. 1).","PeriodicalId":12247,"journal":{"name":"EXCLI Journal","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10776880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of hepatic effects of okadaic acid in HepaRG human liver cells. 在 HepaRG 人肝细胞中分析 okadaic 酸对肝脏影响的蛋白质组学。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-31 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6458

Leonie T D Wuerger, Greta Birkholz, Axel Oberemm, Holger Sieg, Albert Braeuning

引用次数: 0

First case of Lucio's phenomenon in a lepromatous leprosy patient following COVID-19 viral vector vaccine. 首例接种 COVID-19 病毒载体疫苗的麻风病人出现卢西奥现象。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-30 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6661

Roberto Fernandes Soares-Neto, Geyse Maria Lima da Piedade, Priscila Soares Pereira, Rebeca Yasmin Ribeiro Vieira, Jerocilio Maciel de Oliveira-Júnior, Thalyta Porto Fraga, Martha Débora Lira Tenório, Pedro Dantas Oliveira, Paulo Ricardo Martins-Filho

引用次数: 0

Virtual ergonomics of PPE systems - a standardization perspective. 个人防护设备系统的虚拟人体工程学--标准化视角。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-27 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6727

Peter Bröde, Edith Classen, Jean Léonard, Ronald Heus, Kalev Kuklane

引用次数: 0

Is polio making a comeback? The cost of vaccine hesitancy and the disparity in vaccine coverage. 小儿麻痹症是否卷土重来？疫苗犹豫不决的代价和疫苗覆盖率的差异。

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-26 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6594

Thalles Guilarducci Costa, Rizia Rocha Silva, João Victor Rosa de Freitas, Rodrigo Luiz Vancini, Marilia Santos Andrade, Claudio Andre Barbosa de Lira

引用次数: 0

Anti-inflammatory and anti-fibrotic effects of berberine-loaded liquid crystalline nanoparticles. 负载小檗碱的液晶纳米粒子的抗炎和抗纤维化作用

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-26 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6467

Amlan Chakraborty, Keshav Raj Paudel, Chao Wang, Gabriele De Rubis, Dinesh Kumar Chellappan, Philip Michael Hansbro, Chrishan S Samuel, Kamal Dua

引用次数: 0

How different viruses perturb host cellular machinery via short linear motifs. 不同病毒如何通过短线性图案扰乱宿主细胞机制

IF 4.6 3区生物学

EXCLI Journal Pub Date : 2023-10-26 eCollection Date: 2023-01-01 DOI: 10.17179/excli2023-6328

Sobia Idrees, Keshav Raj Paudel, Tayyaba Sadaf, Philip M Hansbro

引用次数: 0