Experimental Dermatology最新文献

{"title":"Comorbidities associated with discoid lupus erythematosus: A case–control study in the All of Us Research Program","authors":"Mihir K. Patil, Carlos E. Salazar, Krithika Nayudu, Thomas Z. Rohan, Vinod E. Nambudiri","doi":"10.1111/exd.15162","DOIUrl":"https://doi.org/10.1111/exd.15162","url":null,"abstract":"","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis reveals a lower ‘UV signature’ in vitiligo skin compared to non-melanoma skin cancers","authors":"Apeksha Yadav, Nida Parveen, Namratha Puttur, Aayush Gupta, Archana Singh","doi":"10.1111/exd.15164","DOIUrl":"https://doi.org/10.1111/exd.15164","url":null,"abstract":"","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic profiling of psoriasis vulgaris and palmoplantar pustulosis","authors":"Yujin Lee,&nbsp;Seong Rae Kim,&nbsp;Mu Seong Ban,&nbsp;Howard Lee,&nbsp;Joo-Youn Cho,&nbsp;Seong Jin Jo","doi":"10.1111/exd.15159","DOIUrl":"https://doi.org/10.1111/exd.15159","url":null,"abstract":"<p>Psoriasis is a chronic inflammatory skin disorder with various subtypes, including psoriasis vulgaris (PV) and palmoplantar pustulosis (PPP). Metabolomics studies have provided insights into psoriasis pathogenesis. However, research on metabolomic alterations in PV and PPP patients is limited. We aimed to explore and compare the metabolic profiles of patients with PV and PPP to those of healthy volunteers (HVs). A single-centre retrospective cohort was constructed, comprising Korean patients with psoriasis and HVs matched by age and sex. Clinical information including demographics, disease severity, and comorbidities were collected. Plasma samples were subjected to targeted metabolic analysis using an Absolute IDQ®p180 kit, which quantified 188 metabolites, including amino acids and carnitines. Statistical significance was assessed using an independent <i>t</i>-test and chi-square test, with <i>p</i>-values adjusted by the Benjamini–Hochberg procedure. Pathway analyses were employed to gain a comprehensive understanding of the metabolite profile. This study included 93 patients (73 PV and 20 PPP) and an equal number of HVs. PV patients showed increased levels of sarcosine, serotonin, propionylcarnitine, proline, aspartic acid, tyrosine, taurine, spermine and ornithine, but exhibited a decreased level of acetylcarnitine than matched HVs. Notably, sarcosine levels were significantly elevated in PPP patients. Furthermore, the sarcosine/glycine ratio was significantly higher in both PV and PPP patients than in HVs. Pathway analysis showed significant increases in metabolites involved in amino acid metabolism and the urea cycle in PV patients. In conclusion, this study demonstrated distinct metabolic profiles in PV and PPP patients compared to HVs, suggesting sarcosine as a potential biomarker for psoriasis.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic optical coherence tomography unveils subclinical, vascular differences across actinic keratosis grades I–III","authors":"Gabriella Fredman,&nbsp;Christine Sofie Krohn Fuchs,&nbsp;Emily Wenande,&nbsp;Peter A. Philipsen,&nbsp;Gavrielle R. Untracht,&nbsp;Flemming Andersen,&nbsp;Peter Bjerring,&nbsp;Stine R. Wiegell,&nbsp;Merete Haedersdal","doi":"10.1111/exd.15153","DOIUrl":"10.1111/exd.15153","url":null,"abstract":"<p>Actinic keratosis (AK) classification relies on clinical characteristics limited to the skin's surface. Incorporating sub-surface evaluation may improve the link between clinical classification and the underlying pathology. We aimed to apply dynamic optical coherence tomography (D-OCT) to characterize microvessels in AK I-III and photodamaged (PD) skin, thereby exploring its utility in enhancing clinical and dermatoscopic AK evaluation. This explorative study assessed AK I-III and PD on face or scalp. AK were graded according to the Olsen scheme before assessment with dermatoscopy and D-OCT. On D-OCT, vessel shapes, −pattern and -direction were qualitatively evaluated at predefined depths, while density and diameter were quantified. D-OCT's ability to differentiate between AK grades was compared with dermatoscopy. Forty-seven patients with AK I-III (<i>n</i> = 207) and PD (<i>n</i> = 87) were included. Qualitative D-OCT evaluation revealed vascular differences between AK grades and PD, particularly at a depth of 300 μm. The arrangement of vessel shapes around follicles differentiated AK II from PD (OR = 4.75, <i>p</i> &lt; 0.001). Vessel patterns varied among AK grades and PD, showing structured patterns in AK I and PD, non-specific in AK II (OR = 2.16,<i>p</i> = 0.03) and mottled in AK III (OR = 29.94, <i>p</i> &lt; 0.001). Vessel direction changed in AK II-III, with central vessel accentuation and radiating vessels appearing most frequently in AK III. Quantified vessel density was higher in AK I-II than PD (<i>p</i> ≤ 0.025), whereas diameter remained constant. D-OCT combined with dermatoscopy enabled precise differentiation of AK III versus AK I (AUC = 0.908) and II (AUC = 0.833). The qualitative and quantitative evaluation of vessels on D-OCT consistently showed increased vascularization and vessel disorganization in AK lesions of higher grades.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfuric acid-induced skin neoplasms in immunocompetent mice","authors":"Oumaima Chgari,&nbsp;Farida Marnissi,&nbsp;Fatimazahra Moukhfi,&nbsp;Martin Ndayambaje,&nbsp;Hicham Wahnou,&nbsp;Youness Limami,&nbsp;Mehdi Karkouri,&nbsp;Mounia Oudghiri","doi":"10.1111/exd.15156","DOIUrl":"10.1111/exd.15156","url":null,"abstract":"<p>This study investigates the carcinogenic potential of chronic dermal exposure (16 weeks) to sulfuric acid (SA) in immunocompetent mice. Clinical assessments, histopathological analyses, immunohistochemical analyses and biochemical assays were conducted to evaluate skin irritation, oxidative stress biomarkers and the potential carcinogenic effect of SA. Results indicated that prolonged exposure to SA leads to various alterations in skin structure, notably inflammation, preneoplastic and neoplastic proliferation in hair follicles, as well as hyperkeratosis and acanthosis, resulting in an increased epidermal thickness of 98.50 ± 21.6 μm. Immunohistochemistry analysis further corroborates these observations, showcasing elevated nuclear expression of p53 and Ki-67, with a significant mitotic index of (57.5% ± 2.5%). Moreover, biochemical analyses demonstrate that SA induces lipid peroxidation in the skin, evidenced by a high level of Malondialdehyde and a consequential reduction in catalase activity. These findings suggest that prolonged exposure to SA can induce skin neoplasms, highlighting the need for stringent safety measures in environments where SA is frequently used. This study underscores the potential occupational health risks associated with SA exposure.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Irisin promotes hair growth and hair cycle transition by activating the GSK-3β/β-catenin pathway","authors":"Yujin Kim,&nbsp;Jung Min Lee,&nbsp;You Na Jang,&nbsp;A. Yeon Park,&nbsp;Su-Young Kim,&nbsp;Beom Joon Kim,&nbsp;Jung Ok Lee","doi":"10.1111/exd.15155","DOIUrl":"10.1111/exd.15155","url":null,"abstract":"<p>Hair loss affects men and women of all ages. Myokines, which are mainly secreted by skeletal muscles during exercise, have numerous health benefits. VEGF, IGF-1, FGF and irisin are reprehensive myokines. Although VEGF, IGF-1 and FGF are positively associated with hair growth, few studies have researched the effects of irisin on hair growth. Here, we investigated whether irisin promotes hair growth using in vitro, ex vivo and in vivo patch assays, as well as mouse models. We show that irisin increases proliferation, alkaline phosphatase (ALP) activity and mitochondrial membrane potential in human dermal papilla cells (hDPCs). Irisin activated the Wnt/β-catenin signalling pathway, thereby upregulating Wnt5a, Wnt10b and LEF-1, which play an important role in hair growth. Moreover, irisin enhanced human hair shaft elongation. In vivo, patch assays revealed that irisin promotes the generation of new hair follicles, accelerates entry into the anagen phase, and significantly increases hair growth in C57BL/6 mice. However, XAV939, a Wnt/β-catenin signalling inhibitor, suppressed the irisin-mediated increase in hair shaft and hair growth. These results indicate that irisin increases hair growth via the Wnt/β-catenin pathway and highlight its therapeutic potential in hair loss treatment.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of S100 proteins and their functions in skin homeostasis, interface dermatitis conditions and other skin pathologies","authors":"Warda Abdi,&nbsp;Andrew Romasco,&nbsp;Dany Alkurdi,&nbsp;Elise Santacruz,&nbsp;Isabel Okinedo,&nbsp;Yuying Zhang,&nbsp;Shriya Kannan,&nbsp;Saeed Shakiba,&nbsp;Jillian M. Richmond","doi":"10.1111/exd.15158","DOIUrl":"10.1111/exd.15158","url":null,"abstract":"<p>S100 proteins comprise a family of structurally related proteins that are calcium-sensitive. S100 proteins have been found to play various roles in regulation of cell apoptosis, cell proliferation and differentiation, cell migration and invasion, energy metabolism, calcium homeostasis, protein phosphorylation, anti-microbial activity and inflammation in a variety of cell types. While the specific function of many S100 proteins remains unknown, some of the S100 proteins serve as disease biomarkers as well as possible therapeutic targets in skin diseases. Interface dermatitis (ID) is a histopathological term that covers many different skin conditions including cutaneous lupus erythematosus, lichen planus, and dermatomyositis. These pathologies share similar histological features, which include basal cell vacuolization and lymphocytic infiltration at the dermal-epidermal junction. In this review, we summarize how the S100 protein family contributes to both homeostatic and inflammatory processes in the skin. We also highlight the role of S100 proteins in neuronal signalling, describing how this might contribute to neuroimmune interactions in ID and other skin pathologies. Last, we discuss what is known about the S100 family proteins as both biomarkers and potential treatment targets in specific pathologies.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pro-inflammatory activity of Cutibacterium acnes phylotype IA1 and extracellular vesicles: An in vitro study","authors":"Caroline T. Cheung,&nbsp;Ugo Lancien,&nbsp;Stéphane Corvec,&nbsp;Valérie Mengeaud,&nbsp;Céline Mias,&nbsp;Joëlle Véziers,&nbsp;Amir Khammari,&nbsp;Brigitte Dréno","doi":"10.1111/exd.15150","DOIUrl":"10.1111/exd.15150","url":null,"abstract":"<p>Acne is a chronic inflammatory skin condition that involves <i>Cutibacterium acnes</i> (<i>C. acnes</i>), which is classified into six main phylotypes (IA₁, IA₂, IB, IC, II and III). Acne development is associated with loss of <i>C. acnes</i> phylotype diversity, characterised by overgrowth of phylotype IA₁ relative to other phylotypes. It was also shown that purified extracellular vesicles (EVs) secreted by <i>C. acnes</i> can induce an acne-like inflammatory response in skin models. We aimed to determine if the inflammatory profile of EVs secreted by <i>C. acnes</i> phylotype IA₁ from an inflammatory acne lesion was different from <i>C. acnes</i> phylotype IA₁ from normal skin, thus playing a direct role in the severity of inflammation. EVs were produced in vitro after culture of two clinical strains of <i>C. acnes</i> phylotype IA₁, T5 from normal human skin and A47 from an inflammatory acne lesion, and then incubated with either human immortalised keratinocytes, HaCaT cells, or skin explants obtained from abdominoplasty. Subsequently, quantitative PCR (qPCR) was performed for human β-defensin 2 (hBD2), cathelicidin (LL-37), interleukin (IL)-1β, IL-6, IL-8, IL-17α and IL-36γ, and ELISA for IL-6, IL-8 and IL-17α. We found that EVs produced in vitro by <i>C. acnes</i> derived from inflammatory acne lesions significantly increased the pro-inflammatory cytokines and anti-microbial peptides at both transcriptional and protein levels compared with EVs derived from normal human skin. We show for the first time that <i>C. acnes</i> EVs from inflammatory acne play a crucial role in acne-associated inflammation in vitro and that <i>C. acnes</i> phylotype IA₁ collected from inflammatory acne lesion and normal skin produce different EVs and inflammatory profiles in vitro.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone modification in psoriasis: Molecular mechanisms and potential therapeutic targets","authors":"Ruifeng Liu,&nbsp;Luyao Zhang,&nbsp;Kaiming Zhang","doi":"10.1111/exd.15151","DOIUrl":"10.1111/exd.15151","url":null,"abstract":"<p>Psoriasis is an immune-mediated, inflammatory disease. Genetic and environmental elements are involved in the nosogenesis of this illness. Epigenetic inheritance serves as the connection between genetic and environmental factors. Histone modification, an epigenetic regulatory mechanism, is implicated in the development of numerous diseases. The basic function of histone modification is to regulate cellular functions by modifying gene expression. Modulation of histone modification, such as regulation of enzymes pertinent to histone modification, can be an alternative approach for treating some diseases, including psoriasis. Herein, we reviewed the regulatory mechanisms and biological effects of histone modifications and their roles in the pathogenesis of psoriasis.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple tape-stripping method for highly reliable and quantitative analysis of skin microbiome","authors":"Naoki Soga,&nbsp;Risa Nagura,&nbsp;Risa Nakamura,&nbsp;Katsunori Kohda,&nbsp;Yumi Motoyama,&nbsp;Masakazu Ito,&nbsp;Ichiro Nakagawa,&nbsp;Saeko Nakajima,&nbsp;Akinori Ikeuchi","doi":"10.1111/exd.15154","DOIUrl":"10.1111/exd.15154","url":null,"abstract":"<p>The composition of human skin microbiome profoundly impacts host skin health and disease. However, the relationship between skin homeostasis or the development of skin diseases and daily changes in skin microbial composition is poorly understood. Longitudinal samplings at more frequent intervals would address this issue, while conventional sampling methods have technical difficulties, leading to limitations in sampling opportunities. Here, we developed a simple and stable tape-stripping method regardless of the operator's skill. Our method enables skin microbial sampling within 30 seconds and taking multiple skin microbial samples from the same body site. The amount of microbial DNA among multiple sampling sites could be measured within 13.5%. The sequencing results of multiple sampling showed high consistency, Pearson's correlation coefficient between multiple samples of 0.98. Furthermore, these results were comparable to those collected by the conventional swabbing method. These results demonstrate that our tape-stripping method enables simple microbiome collection and highly reliable quantitative skin microbiome analysis. These features of our method would lead to a further understanding of skin disease development or diagnosis of skin conditions in clinical research by increasing the opportunities for microbial sampling.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}