FEMS immunology and medical microbiology最新文献_第6页

Inhibition of extracellular signal-regulated kinase 1/2 augments nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells 抑制细胞外信号调节激酶1/2可增加脂多糖刺激的RAW264.7巨噬细胞中一氧化氮的产生

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.03.012

Naoki Koide, Hiroyasu Ito, Mya Mya Mu, Tsuyoshi Sugiyama, Ferdaus Hassan, Shamima Islam, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

{"title":"Inhibition of extracellular signal-regulated kinase 1/2 augments nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells","authors":"Naoki Koide, Hiroyasu Ito, Mya Mya Mu, Tsuyoshi Sugiyama, Ferdaus Hassan, Shamima Islam, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi","doi":"10.1016/j.femsim.2005.03.012","DOIUrl":"10.1016/j.femsim.2005.03.012","url":null,"abstract":"<div>The present study was conducted to determine effects of U0126, a specific inhibitor of mitogen-activated kinase kinase 1/2, on production of nitric oxide (NO) in RAW264.7 macrophage cells. U0126 significantly enhanced NO production in lipopolysaccharide (LPS) but not CpG DNA or interferon-γ-stimulated RAW264.7 cells. In contrast, U0124, a negative control for U0126, did not affect LPS-induced NO production. Further, a series of inhibitors of p38, phosphatidyl-inositol 3-kinase and Janus tyrosine kinase rather caused suppression in LPS-stimulated RAW264.7 cells. U0126 was found to definitely inhibit phosphorylation of extracellular signal-regulated kinase (Erk) 1/2 and augment the levels of inducible type of NO synthase. Antisense oligonucleotides of Erk1/2 also augmented LPS-induced NO production. Inactivation of Erk1/2 by U0126 furthermore inhibited LPS-induced activating protein-1 activation, but not nuclear factor-κB activation. The results suggest that Erk1/2 might negatively regulate NO production in LPS-stimulated RAW264.7 cells.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 2","pages":"Pages 213-219"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25218425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

书评

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.06.002

Alex van Belkum

引用次数: 0

The Shigella flexneri serotype Y vaccine candidate SFL124 originated from a serotype 2a background 福氏志贺氏菌Y血清型候选疫苗SFL124起源于血清型2a背景

FEMS immunology and medical microbiology Pub Date : 2005-08-01 DOI: 10.1016/j.femsim.2005.05.002

Fleur Roberts, Amy V. Jennison, Naresh K. Verma

引用次数: 8

Molecular heterogeneity in Yersinia enterocolitica and ‘Y. enterocolitica-like’ species – Implications for epidemiology, typing and taxonomy 小肠结肠炎耶尔森菌和Y型耶尔森菌的分子异质性。肠结肠炎样物种——流行病学、分型和分类学意义

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.03.006

Jugsharan S. Virdi, Pooja Sachdeva

{"title":"Molecular heterogeneity in Yersinia enterocolitica and ‘Y. enterocolitica-like’ species – Implications for epidemiology, typing and taxonomy","authors":"Jugsharan S. Virdi, Pooja Sachdeva","doi":"10.1016/j.femsim.2005.03.006","DOIUrl":"10.1016/j.femsim.2005.03.006","url":null,"abstract":"<div>Yersinia enterocolitica is an extremely heterogeneous species. Serotyping and biotyping have been used extensively, in the past, to study its heterogeneity and epidemiology. Application of methods like ribotyping, pulsed-field gel electrophoresis and a host of other genomic techniques have further revealed molecular heterogeneity in this species. Furthermore, these methods may be used effectively to supplement serotyping and biotyping schema for studying epidemiology of Y. enterocolitica. This is evident from the ability of some of these methods to subtype strains belonging to serogroups O:3, O:9 and O:8 – which are most commonly encountered in human Yersiniosis. Multilocus enzyme electrophoresis and nucleotide sequencing have reiterated the taxonomic relationships of this organism. However there is paucity of information about the molecular heterogeneity of ‘Y. enterocolitica-like’ species, which need to be addressed in the future. Also, newer techniques such as amplified fragment length polymorphism, VNTR-based typing and multilocus sequence typing should be applied to further understand epidemiology, population structure and evolutionary genetics of Y. enterocolitica and ‘Y. enterocolitica-like’ species.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25160264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Identification of glyceraldehyde-3-phosphate dehydrogenase of epithelial cells as a second molecule that binds to Porphyromonas gingivalis fimbriae 上皮细胞甘油醛-3-磷酸脱氢酶作为结合牙龈卟啉单胞菌菌毛的第二分子的鉴定

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.01.006

Hakimuddin T. Sojar, Robert J. Genco

{"title":"Identification of glyceraldehyde-3-phosphate dehydrogenase of epithelial cells as a second molecule that binds to Porphyromonas gingivalis fimbriae","authors":"Hakimuddin T. Sojar, Robert J. Genco","doi":"10.1016/j.femsim.2005.01.006","DOIUrl":"10.1016/j.femsim.2005.01.006","url":null,"abstract":"<div>Binding of Porphyromonas gingivalis to the host cells is an essential step in the pathogenesis of periodontal disease. P. gingivalis binds to and invades epithelial cells, and fimbriae are thought to be involved in this process. In our earlier studies, two major epithelial cell components of 40 and 50 kDa were identified as potential fimbrial receptors. Sequencing of a cyanogen bromide digestion fragment of the 50-kDa component resulted in an internal sequence identical to keratin I molecules, and hence this cytokeratin represents one of the epithelial cell receptors for P. gingivalis fimbriae. In this study, the 40-kDa component of KB cells was isolated and its amino-terminal sequence determined. The N-terminal amino sequence was found to be GKVKVGVNGF and showed perfect homology with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, purified P. gingivalis fimbriae were found to bind to rabbit muscle GAPDH. Antibodies directed against internal peptide 49–68 and 69–90 of fimbrillin were shown to inhibit the binding of P. gingivalis and of fimbriae to epithelial cells. Antibodies against these peptides also inhibited the binding of fimbriae to GAPDH. Our results confirmed that the amino-terminal domain corresponding to amino residues 49–68 of the fimbrillin protein is the major GAPDH binding domain. These studies point to GAPDH as a major receptor for P. gingivalis major fimbriae and, as such, GAPDH likely plays a role in P. gingivalis adherence and colonization of the oral cavity, as well as triggering host cell processes involved in the pathogenesis of P. gingivalis infections.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 1","pages":"Pages 25-30"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25160267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Transcriptional regulation of β-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells 脂多糖对人宫颈癌(HeLa)细胞β-防御素-2的转录调控

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.01.008

Junji Mineshiba, Fumio Myokai, Fumi Mineshiba, Kaori Matsuura, Fusanori Nishimura, Shogo Takashiba

{"title":"Transcriptional regulation of β-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells","authors":"Junji Mineshiba, Fumio Myokai, Fumi Mineshiba, Kaori Matsuura, Fusanori Nishimura, Shogo Takashiba","doi":"10.1016/j.femsim.2005.01.008","DOIUrl":"10.1016/j.femsim.2005.01.008","url":null,"abstract":"<div>Human β-defensin-2 (hBD-2) is an antimicrobial peptide with a broad spectrum of antimicrobial activity against bacteria, yeast and fungi. Here, we analyzed the transcriptional regulation of hBD-2 in cultured human cervical carcinoma (HeLa) cells with or without lipopolysaccharide (LPS). DNA from position −329 to −39 in the hBD-2 promoter region contained the consensus binding sites for transcription factors, one site for nuclear factor for IL-6 expression (NF-IL6) and two sites for nuclear factor-κB (NF-κB). Reporter gene assays for promoter activity revealed that the region had the highest level of responsiveness to LPS. Furthermore, mutations in both of the NF-κB binding sites caused a significant reduction of the responsiveness to LPS, whereas mutation in the NF-IL6 binding site resulted in an elevation of the basal promoter activity. Electrophoretic mobility shift assays demonstrated that LPS induced the binding of HeLa nuclear factors to 60-bp probe containing the two NF-κB binding sites, suggesting that the sites were essential for the binding. Our results suggest that the two NF-κB binding sites contribute to LPS-mediated hBD-2 transcription while the NF-IL6 binding site represses LPS-independent hBD-2 transcription in the HeLa cells.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 1","pages":"Pages 37-44"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25160269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis 副猪嗜血杆菌中TonB区域的鉴定和表征及其在转铁蛋白介导的铁获取中的作用

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.02.008

María Luisa del Río , César B. Gutiérrez-Martín , José I. Rodríguez-Barbosa , Jesús Navas , Elías F. Rodríguez-Ferri

{"title":"Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis","authors":"María Luisa del Río , César B. Gutiérrez-Martín , José I. Rodríguez-Barbosa , Jesús Navas , Elías F. Rodríguez-Ferri","doi":"10.1016/j.femsim.2005.02.008","DOIUrl":"10.1016/j.femsim.2005.02.008","url":null,"abstract":"<div>Haemophilus parasuis is the causative agent of Glässer’s disease, which is responsible for considerable economic losses in the pig-rearing industry. The aim of the study reported here was the identification, sequencing and molecular characterization of the TonB region that includes tonB, exbBD, and tbpBA genes in H. parasuis. In addition, two fusion proteins were generated. One of them (pGEX-6P-1-GST-TbpB) contained the first 501 amino acids of H. parasuis TbpB protein, while the second (pBAD-Thio-TbpB-V5-His) included the first 102 amino acids of H. parasuis TbpB N-terminus domain. A panel of 14 hybridomas secreting monoclonal antibodies was raised against the two recombinant TbpB fusion proteins. Furthermore, to assess whether the expression of the H. parasuis ExbB, TbpB, and TbpA proteins was upregulated under conditions of restricted availability of iron, a rabbit polyclonal antibody against H. parasuis TbpB-His fusion protein was produced. A rabbit polyclonal antibody against serotype 7 of Actinobacillus pleuropneumoniae ExbB and TbpA proteins was also used for the detection of the homologous proteins in H. parasuis. Overall, the data indicate that H. parasuis, like other members of the Pasteurellaceae family, possesses the genetic elements of the TonB region for iron acquisition and the transferrin-binding proteins encoded under this region are upregulated under restricted iron availability.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 1","pages":"Pages 75-86"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.02.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25161226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

The role of genome diversity and immune evasion in persistent infection with Helicobacter pylori 基因组多样性和免疫逃避在幽门螺杆菌持续感染中的作用

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.04.002

Cara L. Cooke , Jennifer L. Huff , Jay V. Solnick

引用次数: 47

Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries 亚洲国家肺炎链球菌分离株的表型和基因型差异

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI: 10.1016/j.femsim.2005.01.012

Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song

{"title":"Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries","authors":"Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song","doi":"10.1016/j.femsim.2005.01.012","DOIUrl":"10.1016/j.femsim.2005.01.012","url":null,"abstract":"<div>Non-typeable isolates of Streptococcus pneumoniae collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, multilocus sequence typing of housekeeping genes, amplification of virulence-related genes, 16S rDNA-RsaI digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of recP and xpt from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and lytA sequences from typical S. pneumoniae strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of S. pneumoniae. These atypical strains may belong to different bacterial species from S. pneumoniae.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 1","pages":"Pages 63-70"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25161224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Author Index Volume 44 作者索引第44卷

FEMS immunology and medical microbiology Pub Date : 2005-06-01 DOI: 10.1016/S0928-8244(05)00101-X

引用次数: 0