Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song
{"title":"亚洲国家肺炎链球菌分离株的表型和基因型差异","authors":"Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song","doi":"10.1016/j.femsim.2005.01.012","DOIUrl":null,"url":null,"abstract":"<div><p>Non-typeable isolates of <span><em>Streptococcus pneumoniae</em></span><span><span><span> collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, </span>multilocus sequence typing of </span>housekeeping genes, amplification of virulence-related genes, 16S rDNA-</span><em>Rsa</em><span>I digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of </span><em>recP</em> and <em>xpt</em> from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and <em>lytA</em> sequences from typical <em>S. pneumoniae</em> strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of <em>S. pneumoniae</em>. These atypical strains may belong to different bacterial species from <em>S. pneumoniae</em>.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.012","citationCount":"17","resultStr":"{\"title\":\"Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries\",\"authors\":\"Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song\",\"doi\":\"10.1016/j.femsim.2005.01.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Non-typeable isolates of <span><em>Streptococcus pneumoniae</em></span><span><span><span> collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, </span>multilocus sequence typing of </span>housekeeping genes, amplification of virulence-related genes, 16S rDNA-</span><em>Rsa</em><span>I digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of </span><em>recP</em> and <em>xpt</em> from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and <em>lytA</em> sequences from typical <em>S. pneumoniae</em> strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of <em>S. pneumoniae</em>. These atypical strains may belong to different bacterial species from <em>S. pneumoniae</em>.</p></div>\",\"PeriodicalId\":12220,\"journal\":{\"name\":\"FEMS immunology and medical microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.012\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEMS immunology and medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928824405000441\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405000441","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries
Non-typeable isolates of Streptococcus pneumoniae collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, multilocus sequence typing of housekeeping genes, amplification of virulence-related genes, 16S rDNA-RsaI digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of recP and xpt from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and lytA sequences from typical S. pneumoniae strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of S. pneumoniae. These atypical strains may belong to different bacterial species from S. pneumoniae.
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