Experimental eye research最新文献_第3页

Proteomic analysis of effects of 1% atropine in myopia therapy in Guinea pigs. 1%阿托品对豚鼠近视治疗效果的蛋白质组学分析。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-25 DOI: 10.1016/j.exer.2024.110224

Chen Chu, Luyao Ye, Qingqing Chi, Jiangnan He, Jianfeng Zhu

{"title":"Proteomic analysis of effects of 1% atropine in myopia therapy in Guinea pigs.","authors":"Chen Chu, Luyao Ye, Qingqing Chi, Jiangnan He, Jianfeng Zhu","doi":"10.1016/j.exer.2024.110224","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110224","url":null,"abstract":"Myopia is a significant global public health issue. Key interventions for managing myopia include atropine treatment, optical correction, and surgical methods. This study focused on evaluating alterations in retinal protein expression after atropine therapy for myopia. Guinea pigs were randomly divided into four groups: control (CON), monocular form-deprivation myopia (FDM), FDM with 2-week atropine treatment (FDM + ATR), and atropine-only treatment (ATR). After two weeks of FDM induction, the FDM group showed significant differences in refractive error and increased axial lengths. In comparing the retinas of myopic and normal eyes, 30 proteins were found to have increased expression, while 8 proteins showed decreased expression. Atropine-treated retinas exhibited 73 proteins with increased expression and 29 proteins with decreased expression compared to the normal eyes. A total of 11 regulated proteins overlapped between the FDM + ATR vs FDM and FDM vs CON groups. IPA analysis indicates significant alterations in amino acid metabolism, energy production, post-translational modification, small molecule biochemistry, and free radical scavenging. Our study identifies retinal protein changes in myopic guinea pigs and in guinea pigs treated with atropine after myopia. These proteins could serve as potential targets for atropine treatment of myopia.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"251 ","pages":"110224"},"PeriodicalIF":3.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring ocular disorders in Parkinson's disease: A comprehensive review and future perspectives. 帕金森氏病眼部疾病的研究：综述与展望

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-24 DOI: 10.1016/j.exer.2024.110225

Minal Thacker, Ka Ying Wong, Liping Zhou, Juewen Liu, Man-Sau Wong

引用次数: 0

Photobiomodulation inhibits retinal degeneration in diabetic mice through modulation of stem cell mobilization and gene expression. 光生物调节通过调节干细胞动员和基因表达抑制糖尿病小鼠视网膜变性。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-22 DOI: 10.1016/j.exer.2024.110218

Jingyan Ge, Yinan Zhang, Ling Han, Liangliang Zhao, Hongwei Zhao, Dan Qiao, Yan Cheng

{"title":"Photobiomodulation inhibits retinal degeneration in diabetic mice through modulation of stem cell mobilization and gene expression.","authors":"Jingyan Ge, Yinan Zhang, Ling Han, Liangliang Zhao, Hongwei Zhao, Dan Qiao, Yan Cheng","doi":"10.1016/j.exer.2024.110218","DOIUrl":"10.1016/j.exer.2024.110218","url":null,"abstract":"The number of people suffering from type 2 diabetes (DM2) is increasing and over 30 percent of DM2 patients will develop diabetic retinopathy (DR). Available therapeutic approaches for DR have their limitations. It is of great significance to search for other effective alternate therapeutic approaches. The present study aimed to explore the beneficial effects of photobiomodulation (PBM) on the diabetic retinopathy and underlying mechanisms. Streptozotocin was administered to male mice to establish diabetic model. The mice in the diabetic group (DM) received no treatment, and the mice in DM + PBM group received LED illumination (wavelength 670 nm) once a day for 20 consecutive weeks. Retinal vessel degenerate changes, the expression levels of E-Cadherin, N-Cadherin and the mRNA levels of c-kit, CXCR4, MYPT1, SCF, SDF1-α in retina, the levels of SDF-1α and SCF in the peripheral blood and the number of LSK cells expressing c-kit and sca-1 were determined. PBM could significantly inhibit the degenerative change of diabetic retinal vessels, decrease the expression levels of E-Cadherin and N-Cadherin and the mRNA levels of c-kit, CXCR4, MYPT1, SCF, SDF1-α and increase VEGF mRNA levels in retina. PBM could also increase the levels of SDF-1α and SCF in the peripheral blood and the number of LSK cells expressing c-kit and sca-1 in diabetic mice. PBM at 4 min/day for 20 consecutive weeks significantly inhibit the degenerative change of diabetic retinal vessels, and PBM is likely to produce its beneficial effects on the retina through promoting the migration of bone marrow stem cells to circulation and diabetic retinal tissue. The present study provides a new therapeutic direction and experimental foundation for the treatment of diabetic retinopathy.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110218"},"PeriodicalIF":3.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How crosstalk between mitochondria, lysosomes, and other organelles can prevent or promote dry age-related macular degeneration. 线粒体、溶酶体和其他细胞器之间的相互作用如何预防或促进干性老年性黄斑变性。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-22 DOI: 10.1016/j.exer.2024.110219

Aparna Lakkaraju, Patricia Boya, Marie Csete, Deborah A Ferrington, James B Hurley, Alfredo A Sadun, Peng Shang, Ruchi Sharma, Debasish Sinha, Marius Ueffing, Susan E Brockerhoff

{"title":"How crosstalk between mitochondria, lysosomes, and other organelles can prevent or promote dry age-related macular degeneration.","authors":"Aparna Lakkaraju, Patricia Boya, Marie Csete, Deborah A Ferrington, James B Hurley, Alfredo A Sadun, Peng Shang, Ruchi Sharma, Debasish Sinha, Marius Ueffing, Susan E Brockerhoff","doi":"10.1016/j.exer.2024.110219","DOIUrl":"10.1016/j.exer.2024.110219","url":null,"abstract":"Organelles such as mitochondria, lysosomes, peroxisomes, and the endoplasmic reticulum form highly dynamic cellular networks and exchange information through sites of physical contact. While each organelle performs unique functions, this inter-organelle crosstalk helps maintain cell homeostasis. Age-related macular degeneration (AMD) is a devastating blinding disease strongly associated with mitochondrial dysfunction, oxidative stress, and decreased clearance of cellular debris in the retinal pigment epithelium (RPE). However, how these occur, and how they relate to organelle function both with the RPE and potentially the photoreceptors are fundamental, unresolved questions in AMD biology. Here, we report the discussions of the \"Mitochondria, Lysosomes, and other Organelle Interactions\" task group of the 2024 Ryan Initiative for Macular Research (RIMR). Our group focused on understanding the interplay between cellular organelles in maintaining homeostasis in the RPE and photoreceptors, how this could be derailed to promote AMD, and identifying where these pathways could potentially be targeted therapeutically.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110219"},"PeriodicalIF":3.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The vectors went in two-by-two: Transduction efficiency and tolerability of dual and triple rAAV vector delivery following intravitreal injection for genome-editing applications. 载体分为两部分：在基因组编辑应用的玻璃体内注射后，双重和三重rAAV载体传递的转导效率和耐受性。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110223

Rachel L Fehrman, Kristina J Chern, Kyle P Stoltz, Daniel M Lipinski

{"title":"The vectors went in two-by-two: Transduction efficiency and tolerability of dual and triple rAAV vector delivery following intravitreal injection for genome-editing applications.","authors":"Rachel L Fehrman, Kristina J Chern, Kyle P Stoltz, Daniel M Lipinski","doi":"10.1016/j.exer.2024.110223","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110223","url":null,"abstract":"Genome or prime editing has become a promising tool for the treatment of hereditary disorders affecting the inner retina, such as dominant optic neuropathies. In vivo delivery of gene editors, such as Cas9, is typically achieved using recombinant adeno-associated virus (rAAV) vectors, which have a broad range of cellular tropisms and are well tolerated following intravitreal administration. Owing to the large size of gene editing constructs and the limited carrying capacity of rAAV (<5.1kb) it is unfortunately usually necessary to split therapeutic transgene cassettes across multiple co-administered vector genomes. While the efficiency with which multiple vector genomes recombine following cellular entry has been studied extensively, another potentially limiting factor is the likelihood of target cells (e.g. retinal ganglion cells) receiving two or more vectors containing genomes that correspond to the full-length expression cassette when recombined. In this study we examine the efficiency with which two or more vector genomes transduce various retinal cell types following intravitreal administration. rAAV2/2[MAX] vectors expressing individual fluorescent reporters (GFP, BFP or mCherry) were co-injected intravitreally singly or in combination (dual or triple), allowing the extent of co-transduction to be assessed through multimodal in vivo imaging, electroretinography, flow cytometry and post-mortem histology. We find that intravitreal co-administration of vectors containing multiple genomes is well tolerated - with no observed alterations in retinal thickness or ERG amplitudes - but that co-transduction efficiency decreases significantly with increasing genome number. As such co-transduction of multiple vectors may be a major bottleneck limiting gene editing of inherited disorders affecting the inner retina.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110223"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of different exercise modalities on oxidative stress in animal models of high intraocular pressure and diabetes. 不同运动方式对高眼压和糖尿病动物模型氧化应激的保护作用。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110216

Sabrina Nau da Silva Piazza, Paula Bortoluzzi Canteiro, Natalia Dos Santos Tramontin, Giulia Strapazzon, Vanessa de Moraes Andrade, Alexandre Pastoris Muller

{"title":"Protective effects of different exercise modalities on oxidative stress in animal models of high intraocular pressure and diabetes.","authors":"Sabrina Nau da Silva Piazza, Paula Bortoluzzi Canteiro, Natalia Dos Santos Tramontin, Giulia Strapazzon, Vanessa de Moraes Andrade, Alexandre Pastoris Muller","doi":"10.1016/j.exer.2024.110216","DOIUrl":"10.1016/j.exer.2024.110216","url":null,"abstract":"High intraocular pressure (HIOP) and high glucose levels are associated with oxidative stress. Although physical exercise protects against oxidative damage, its specific impact on eye health remains unclear. Thus, this study aimed to assess the impact of physical exercise on the oxidative status of whole eyes in male Swiss mice subjected to HIOP model and cafeteria diet (CD). In experiment one, mice were divided into sedentary, aerobic, and strength (four-week physical exercise) groups and subjected to an HIOP/ischemia model. In experiment two, mice were submitted to CD and voluntary physical exercise for 18 weeks, according to the following groups: sedentary control, sedentary CD, exercise control, and exercise CD. Experiment one revealed elevated 2',7'-dichlorodihydrofluorescein (DCFH) levels in aerobic group, which decreased in all groups after ischemia. Nitrite levels were decreased on strength than in sedentary group. The superoxide dismutase (SOD) activity did not change in all treatments. Although catalase (CAT) activity increased in aerobic and strength groups, and after ischemia in all groups. In experiment two, the sedentary CD group presented higher body weight than the other groups. DCFH levels were increased in the exercise control and reduced in the exercise CD compared with the other groups. CAT activity and sulfhydryl groups were decreased, while protein carbonylation was increased in the sedentary CD group compared with the other groups. Thus, these results suggested that physical exercise promoted antioxidant effects on eyes exposed to an HIOP model and CD.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110216"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the IL-6 trans-signaling pathway in the absence or presence of TGF-β2 on Schlemm's canal endothelial cells. TGF-β2缺失或存在时IL-6反式信号通路对施莱姆管内皮细胞的影响

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110215

Mai Urahashi, Tomokazu Fujimoto, Miyuki Inoue-Mochita, Toshihiro Inoue

{"title":"Effect of the IL-6 trans-signaling pathway in the absence or presence of TGF-β2 on Schlemm's canal endothelial cells.","authors":"Mai Urahashi, Tomokazu Fujimoto, Miyuki Inoue-Mochita, Toshihiro Inoue","doi":"10.1016/j.exer.2024.110215","DOIUrl":"10.1016/j.exer.2024.110215","url":null,"abstract":"Intraocular pressure (IOP) is regulated through the balance of production and drainage of aqueous humor. The main route of aqueous-humor outflow comprises the trabecular meshwork (TM) and Schlemm's canal (SC). We reported that IL-6 trans-signaling can inhibit TGF-β signaling in TM cells and may affect regulation of IOP. However, the function of IL-6 trans-signaling in SC cells remains unclear. Therefore, we investigated the role of IL-6 trans-signaling in monkey SC cells. Simultaneous treatment with IL-6 and soluble IL-6 receptor (sIL-6R) significantly decreased the trans-endothelial electrical resistance (TER) of SC cells and reduced aqueous-humor outflow resistance. Moreover, activation of IL-6 trans-signaling significantly reduced expression of fibronectin, ZO-1 and claudin-5, and increased that of several matrix metalloproteinases. We also investigated the effect of IL-6 trans-signaling on TGF-β2-induced changes in SC cells. Simultaneous treatment with IL-6 and sIL-6R significantly suppressed the TGF-β2-induced increase in the TER of SC cells but did not affect the activity of the TGF-β2 signaling pathway. By contrast, the TGF-β2-induced increases in the expression of fibronectin and collagen type I were significantly decreased upon simultaneous treatment with IL-6 and sIL-6R. The results show that IL-6 trans-signaling suppressed TGF-β2-induced increase in outflow resistance.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110215"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SP1/COL1A2/ZEB1 axis promotes TGF-β2-induced lens epithelial cell proliferation, migration, invasion and EMT process. SP1/COL1A2/ZEB1轴促进TGF-β2诱导的晶状体上皮细胞增殖、迁移、侵袭和EMT过程。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110220

Lili Zhao, Ping Wang, Lianyi Sun, Weimei Ma, Lei Yu

{"title":"SP1/COL1A2/ZEB1 axis promotes TGF-β2-induced lens epithelial cell proliferation, migration, invasion and EMT process.","authors":"Lili Zhao, Ping Wang, Lianyi Sun, Weimei Ma, Lei Yu","doi":"10.1016/j.exer.2024.110220","DOIUrl":"10.1016/j.exer.2024.110220","url":null,"abstract":"Posterior capsule opacification (PCO) is the most common complication after cataract surgery. In this study, we used transforming growth factor beta-2 (TGF-β2)-induced SRA01/04 cells to mimic PCO cell model and explored the functions and underlying mechanisms of specific protein 1 (SP1) in TGF-β2-induced SRA01/04 cell development. MTT assay and EdU assay were carried out to explore the proliferation of SRA01/04 cells. Transwell assay and wound-healing assay were performed to investigate SRA01/04 cell migration and invasion. Chromatin Immunoprecipitation (ChIP) assay, dual-luciferase reporter assay and Co-immunoprecipitation (Co-IP) assay were used to analyze the relations of SP1, COL1A2 and ZEB1. TGF-β2 treatment led to the promotion of SRA01/04 cell proliferation, migration, invasion and EMT process. COL1A2 level was induced by TGF-β2 treatment and COL1A2 knockdown inhibited TGF-β2-induced SRA01/04 cell proliferation, migration, invasion and EMT. SP1 could activate the transcription of COL1A2. SP1 overexpression promoted TGF-β2-induced SRA01/04 cell injury by regulating COL1A2 expression. Moreover, COL1A2 interacted with ZEB1 and COL1A2 knockdown-mediated effects on the proliferation, migration, invasion and EMT of TGF-β2-induced SRA01/04 cells were abrogated by elevating ZEB1. SP1 regulated COL1A2 and then mediated ZEB1 to affect the proliferation, migration, invasion and EMT of TGF-β2-induced SRA01/04 cells.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110220"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taurine mechanism in preventing retinal cell damage from acute ocular hypertension through GTPBP3 regulation. 牛磺酸通过调节GTPBP3预防急性高眼压视网膜细胞损伤的机制。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110222

Wei Lu, Yuting Yang, Shunxiang Gao, Jihong Wu, Xinghuai Sun

{"title":"Taurine mechanism in preventing retinal cell damage from acute ocular hypertension through GTPBP3 regulation.","authors":"Wei Lu, Yuting Yang, Shunxiang Gao, Jihong Wu, Xinghuai Sun","doi":"10.1016/j.exer.2024.110222","DOIUrl":"10.1016/j.exer.2024.110222","url":null,"abstract":"We aimed to explore the protective effects and underlying mechanisms of taurine on retinal cells during acute ocular hypertension (AOH)-induced damage. Retinal morphology, apoptosis, mitochondrial structure, electroretinography, expression of GTP binding protein 3 (GTPBP3), and molecules in the unfolded protein response (UPR) were examined in an AOH mouse model and wild-type (WT) mice with or without intravitreal injection of taurine. For in vitro experiments, the GTPBP3 expression and endoplasmic reticulum (ER) stress were examined in R28 cell line under hydrogen peroxide (H2O2)-induced damage or hypoxia/reoxygenation (H/R)-induced damage, with or without taurine pretreatment. Taurine pretreatment alleviated retinal damage caused by AOH modeling. The GTPBP3 expression level decreased after AOH injury, and taurine pretreatment reversed this reduction. Retinas with decreased GTPBP3 expression showed reduced retinal ganglion cell (RGC) function, which could be reversed by intravitreal taurine injection. In H2O2-, H/R-, and AOH-induced damage, UPR were activated and alleviated by taurine pretreatment. GTPBP3 knockdown in R28 cells also activated the UPR, which was alleviated by taurine. A UPR activator downregulated GTPBP3 levels in normal R28 cells, whereas a UPR inhibitor upregulated GTPBP3 levels in GTPBP3 knockdown R28 cells. In conclusion, this study provides important evidence that taurine prevents retinal cell damage in mice exposed to AOH and modulates GTPBP3 expression via the UPR pathway. Interventions targeting this mechanism can be used as potential therapeutic targets for AOH damage.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110222"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testosterone promotes photoreceptor degeneration in the sodium iodate model. 在碘酸钠模型中，睾酮促进光感受器变性。

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110221

Timothy T Lee, Brent A Bell, Ying Song, Joshua L Dunaief

{"title":"Testosterone promotes photoreceptor degeneration in the sodium iodate model.","authors":"Timothy T Lee, Brent A Bell, Ying Song, Joshua L Dunaief","doi":"10.1016/j.exer.2024.110221","DOIUrl":"10.1016/j.exer.2024.110221","url":null,"abstract":"Previously, we found that retinas of young male mice were more damaged than those of young female mice in the sodium iodate (NaIO3) model. The purpose of this study was to test whether reducing testosterone levels would be retina-protective. Male C57Bl/6J mice underwent surgical castration or sham surgery, then were given an intraperitoneal injection of NaIO3 at 25 mg/kg. The mice were imaged a week later using optical coherence tomography (OCT). ImageJ with a custom macro was utilized to measure retinal thicknesses in OCT images. Electroretinography (ERG) was used to measure retinal function one week post-injection. After euthanasia, quantitative real-time PCR (qRT-PCR) was performed. Surgical castration partially protected photoreceptors, which was indicated by less photoreceptor layer thinning exhibited in OCT images compared to the sham surgery group. Consistent with this, qRT-PCR of castration group neural retinas revealed less reduction of rhodopsin mRNAs, and less upregulation of antioxidant as well as glucose transporter 1 mRNAs. ERG results also demonstrated partial preservation of both cone and rod function. These results indicate that surgical castration provided structural and functional protection to photoreceptors against NaIO3. These neuroprotective effects suggest that testosterone may be harmful to the stressed retina. Further investigation of this pathway could lead to a better understanding of the mechanisms involved in retinal degeneration.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110221"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0