Experimental eye research最新文献_第2页

Fluorometholone inhibits corneal epithelial proliferation, migration via targeting Rho GTPases: RhoA, Rac1, and Cdc42 氟美洛酮通过靶向Rho gtpase: RhoA、Rac1和Cdc42抑制角膜上皮的增殖和迁移

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-21 DOI: 10.1016/j.exer.2025.110397

Yong Lin , Tianyi Xu , Qiuruo Jiang, Jialu Chen, Hua Zhang, Peter Sol Reinach, Dongsheng Yan, Jia Qu, Shihao Chen

{"title":"Fluorometholone inhibits corneal epithelial proliferation, migration via targeting Rho GTPases: RhoA, Rac1, and Cdc42","authors":"Yong Lin , Tianyi Xu , Qiuruo Jiang, Jialu Chen, Hua Zhang, Peter Sol Reinach, Dongsheng Yan, Jia Qu, Shihao Chen","doi":"10.1016/j.exer.2025.110397","DOIUrl":"10.1016/j.exer.2025.110397","url":null,"abstract":"<div><div>Abnormal corneal epithelial hyperplasia is a common complication following refractive surgery. 0.1 % fluorometholone (FML) eye drops are commonly used for treatment. However, their efficacy varies among patients, potentially attributed to differences in the patient's microenvironment. The underlying reason remains incompletely understood. This study aimed to elucidate the molecular mechanisms of FML's action on corneal epithelial cells (CECs). The effects of FML on the cell viability, proliferation, cell cycle, and migration of human corneal epithelial cells (HCECs) were evaluated using MTS assay, EdU staining, flow cytometry, and scratch assay, respectively. Mouse corneal sections were immunofluorescently stained to assess cell proliferation. A corneal wound model, monitored by slit-lamp photography, was utilized to evaluate the impact of FML on wound healing. Gene expression alterations were detected via RNA sequencing. RT-qPCR and Western blot were employed to validate gene and protein expression in HCECs and mouse corneal epithelia. Proteomic analysis was conducted on tear samples from patients. FML treatment significantly inhibited CEC proliferation, migration, and wound healing. At the molecular level, FML treatment led to a remarkable downregulation of RhoA, Rac1, and Cdc42. Correspondingly, reductions in the downstream Erk and NF-κB signaling pathways were observed in both HCECs and mouse corneal epithelia. Moreover, these pathways were similarly downregulated in tear samples from clinical patients. In conclusion, FML inhibits CEC proliferation and migration by modulating the Rho GTPase signaling network, especially through RhoA/Rac1/Cdc42, thereby suppressing the Erk/NF-κB pathway.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"256 ","pages":"Article 110397"},"PeriodicalIF":3.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of highly and extremely aberrated wavefront for ocular Shack-Hartmann sensor using multi-task Attention-UNet 基于多任务注意- unet的高像差和极像差眼传感器波前重建

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-18 DOI: 10.1016/j.exer.2025.110394

Yibin Tian , Zipei Luo , Dajiang Lu , Cheng Liu , Christine Wildsoet

{"title":"Reconstruction of highly and extremely aberrated wavefront for ocular Shack-Hartmann sensor using multi-task Attention-UNet","authors":"Yibin Tian , Zipei Luo , Dajiang Lu , Cheng Liu , Christine Wildsoet","doi":"10.1016/j.exer.2025.110394","DOIUrl":"10.1016/j.exer.2025.110394","url":null,"abstract":"<div><div>In certain ocular conditions, such as in eyes with keratoconus or after corneal laser surgery, Higher Order Aberrations (HOAs) may be dramatically elevated. Accurately recording interpretable wavefronts in such highly aberrated eyes using Shack-Hartmann sensor is a challenging task. While there are studies that have applied deep neural networks to Shack-Hartmann wavefront reconstructions, they have been limited to low resolution and small dynamic range cases. In this study, we introduce a multi-task learning scheme for High-Resolution and High Dynamic Range Shack-Hartmann wavefront reconstruction using a modified attention-UNet (HR-HDR-SHUNet), which outputs a wavefront map along with Zernike coefficients simultaneously. The HR-HDR-SHUNet was evaluated on three large datasets with different levels of HOAs (regularly, highly, and extremely aberrated), with successful reconstruction of all aberrated wavefronts, at the same time achieving significantly higher accuracy than both traditional methods and other deep learning networks; it is also computationally more efficient than the latter.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110394"},"PeriodicalIF":3.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

APEX1 attenuates ERS-induced paraptosis by inhibiting the P53 pathway in LECs APEX1通过抑制lec中的P53通路减弱ers诱导的细胞凋亡

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-18 DOI: 10.1016/j.exer.2025.110393

Renhao Zhong , Lihua Kang , Wenjing Geng, Linhui Xu, Pengfei Li, Miaomiao Wu, Guowei Zhang, Mengying Zhou, Kai Zhang, Min Ji, Huaijin Guan

{"title":"APEX1 attenuates ERS-induced paraptosis by inhibiting the P53 pathway in LECs","authors":"Renhao Zhong , Lihua Kang , Wenjing Geng, Linhui Xu, Pengfei Li, Miaomiao Wu, Guowei Zhang, Mengying Zhou, Kai Zhang, Min Ji, Huaijin Guan","doi":"10.1016/j.exer.2025.110393","DOIUrl":"10.1016/j.exer.2025.110393","url":null,"abstract":"<div><div>Age-related cortical cataract (ARCC) is a prominent subtype of cataract, characterized by the presence of vacuoles and spoke-like opacity. Previous studies have suggested that paraptosis is involved in the onset of early ARCC vacuolar degeneration. In this experiment, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced SRA01/04 cells were used to establish a paraptosis-like cell model, and the function and underlying mechanism of APEX1 in this cell model were explored. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western Blot analyses were conducted to assess the expression of pertinent genes in SRA01/04. Confocal fluorescence microscopy, using ER-tracker kits, was applied to clarify the relationship between the endoplasmic reticulum and intracellular vacuoles. The co-IP assay was used to verify the interaction between APEX1 and P53. The GTRD database was employed to predict the putative target genes combined with P53, and CUT&RUN assay was employed to confirm the enrichment of the P53 and ATF6 promoters following APEX1 overexpression. Firstly, the pathological sections of the vacuolar degeneration zone in the lens cortex of ARCC patients exhibited fiber disarray and vacuole development. Meanwhile, the protein expression of Alix, a specific paraptosis inhibitor, was decreased in low-concentration H<sub>2</sub>O<sub>2</sub>-treated SRA01/04 cells. Secondly, we discovered that 4-PBA suppressed the expression of ATF6 and PERK. Moreover, overexpression of APEX1 in SRA01/04 cells improved endoplasmic reticulum morphology, inhibited the interaction between P53 and ATF6, and attenuated paraptosis in SRA01/04. APEX1 regulated P53 and then mediated ATF6 to affect the endoplasmic reticulum stress and paraptosis in H<sub>2</sub>O<sub>2</sub>-induced SRA01/04 cells.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110393"},"PeriodicalIF":3.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limbal explant cultures on amniotic membrane: The effects of passaging the explants on cell phenotype 羊膜边缘外植体培养：外植体传代对细胞表型的影响

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-16 DOI: 10.1016/j.exer.2025.110392

Mehmet Gurdal , Kemal Baysal , Ismet Durak , Ozlem Barut Selver

{"title":"Limbal explant cultures on amniotic membrane: The effects of passaging the explants on cell phenotype","authors":"Mehmet Gurdal , Kemal Baysal , Ismet Durak , Ozlem Barut Selver","doi":"10.1016/j.exer.2025.110392","DOIUrl":"10.1016/j.exer.2025.110392","url":null,"abstract":"<div><div><em>In vitro</em> expansion of limbal epithelial stem cells (LESCs) while maintaining their characteristics has the potential to address the urgent need in ophthalmology clinics for the treatment of limbal stem cell deficiency (LSCD). Herein, we investigated the impact of explant passaging on the phenotype of LESCs cultured on human amniotic membrane (hAM). Following initial coverage of the hAM surface by cells (passage 0), the rabbit limbal explants underwent two additional passages. Expanded cells were then counted using a hemocytometer and examined by immunocytochemistry and RT-qPCR to assess markers associated with LESCs (ABCG2, P63, CK14, CXCR4, BMI-1, and vimentin) and differentiated LESCs (CK3 and connexin 43). The cell yield of passage 1 was the highest among all passages. Immunocytochemistry analysis revealed that the number of CK14-positive cells was similar across all passages; vimentin-positive cells were the lowest in passage 0, while vimentin-positive cells were the highest in passage 1; and CK3-positive cells were the highest in passage 0. RT-qPCR analysis revealed that CK3 and connexin 43 expression was significantly higher in passage 0 cells than in passage 2 cells; and CXCR4 and BMI-1 expressions were significantly higher in passage 1 cells than in passage 0 cells. Our data highlight that the passaging of limbal explant on hAM results in varying cell characteristics. The decrease in CK3 and increase in ABCG2 expression in cells obtained by passaging the limbal explant suggest that passaging could potentially enhance the stem cell population within the <em>in vitro</em> limbal explant culture on hAM.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110392"},"PeriodicalIF":3.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of herpes simplex virus type 1 infection by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry 基质辅助激光解吸/电离飞行时间质谱法鉴定1型单纯疱疹病毒感染

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-15 DOI: 10.1016/j.exer.2025.110391

Chao Cheng , Yan Zong , Fang Duan , Ziyan Chen , Xiuping Liu , Kaili Wu

{"title":"Identification of herpes simplex virus type 1 infection by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry","authors":"Chao Cheng , Yan Zong , Fang Duan , Ziyan Chen , Xiuping Liu , Kaili Wu","doi":"10.1016/j.exer.2025.110391","DOIUrl":"10.1016/j.exer.2025.110391","url":null,"abstract":"<div><div>This study aimed to investigate whether Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) could identify Herpes simplex virus type 1 (HSV1) infection in samples <em>in vitro</em> and <em>in vivo</em>. MS spectra of supernatants and suspensions from infected human cornea epithelial (HCE) cell culture samples and infected samples of BALB/c mouse corneas were obtained by a VITEK® mass spectrometer. The discriminating peaks between infected and non-infected samples were used to establish discriminating superspectra (DSPc for cells and DSPm for corneas) by SARAMIS™ software. Another infected cells with two viral titers and infected cornea samples were used for blind testing against two DSPs. The results showed that automatic matching by the SARAMIS system revealed 28 discriminating peaks in HSV1-infected cells and 17 discriminating peaks in HSV1 keratitis, generating two discriminating superspectra (DSPs). Blind testing of virus-infected samples demonstrated a high positive identification rate for both <em>in vitro</em> and <em>in vivo</em> DSPs. The positive identification rate varied with viral titers, with cell suspensions exhibiting significantly higher rates compared to supernatants. Cluster analysis based on MS spectra revealed that there were more obvious differences between <em>in vivo</em> and <em>in vitro</em> samples compared to the differences between infected and non-infected samples. These findings suggest that MALDI-TOF MS can directly identify HSV1 <em>in vitro</em> or <em>in vivo</em> infected specimens, with higher positivity rates achieved when using cellular suspensions directly. This is an attempt on the method of virus detection, which shows potential for using MS to detect HSV1 infection or other virus infection in humans.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110391"},"PeriodicalIF":3.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical application of Cap-loaded hydrogels inhibits corneal neovascularization 局部应用帽负载水凝胶抑制角膜新生血管

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-15 DOI: 10.1016/j.exer.2025.110390

Xiaoyang Xue , Xiaochuan Duan , Mengyuan Qin , Shoukuan Liu , Lin Su , Xin Cao , Hongquan Duan , Boshi Liu , Tianwen Ni , Xiaorong Li

{"title":"Topical application of Cap-loaded hydrogels inhibits corneal neovascularization","authors":"Xiaoyang Xue , Xiaochuan Duan , Mengyuan Qin , Shoukuan Liu , Lin Su , Xin Cao , Hongquan Duan , Boshi Liu , Tianwen Ni , Xiaorong Li","doi":"10.1016/j.exer.2025.110390","DOIUrl":"10.1016/j.exer.2025.110390","url":null,"abstract":"<div><div>Corneal neovascularization (CNV) is a significant risk factor for visual impairment. The efficiency and side effects of current CNV treatments, such as steroids and antivascular endothelial growth factor agents, are still debated. In addition, the bioavailability of topical drugs is usually hindered by tears, blinking, and the corneal anatomy. Therefore, finding a new therapeutic strategy is important. This study aimed to examine the function of the new therapeutic agent capmatinib (Cap), a highly selective inhibitor of MET that plays an important role in angiogenesis, in treating CNV. In this study, we first investigated the role of the HGF/c-MET axis in CNV and the therapeutic effect of Cap in a corneal alkali burn model. We synthesised a genipin-crosslinked gelatine-based hydrogel containing Cap (Cap-Gel). We observed a more significant therapeutic effect with the Cap-Gel than with Cap alone, as well as the alleviation of inflammatory infiltration and fibrosis. On day 14, the Cap-Gel group showed the most significant inhibition of corneal neovascularization, with the shortest neovessel length (0.48 ± 0.13 mm), smallest CNV area (3.77 ± 0.78 mm<sup>2</sup>), and lowest clinical assessment score (3.33 ± 0.52). Taken together, our results suggest that Cap-Gel could be a promising drug candidate for treating CNV.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110390"},"PeriodicalIF":3.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-omics study reveals molecular characteristics and therapeutic targets of salidroside in reducing TGF-β2-induced ECM expression 一项多组学研究揭示了红景天苷降低TGF-β2诱导的ECM表达的分子特征和治疗靶点

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-10 DOI: 10.1016/j.exer.2025.110386

Rong Zhang , Ning Li , Yuanfu Fan , Dai Qing , Sijie Zhao , Xiaohui Ren , Aiqin Wang , Ziqing Gao , Yuchen Fan

{"title":"A multi-omics study reveals molecular characteristics and therapeutic targets of salidroside in reducing TGF-β2-induced ECM expression","authors":"Rong Zhang , Ning Li , Yuanfu Fan , Dai Qing , Sijie Zhao , Xiaohui Ren , Aiqin Wang , Ziqing Gao , Yuchen Fan","doi":"10.1016/j.exer.2025.110386","DOIUrl":"10.1016/j.exer.2025.110386","url":null,"abstract":"<div><div>Primary open-angle glaucoma (POAG) is a leading cause of irreversible blindness worldwide, driven by elevated intraocular pressure (IOP) due to trabecular meshwork (TM) fibrosis, extracellular matrix (ECM) accumulation, and increased aqueous humor outflow resistance. Transforming growth factor-beta 2 (TGF-β2) promotes the expression of fibrosis-related genes, exacerbating these effects. Salidroside, a bioactive compound, has been shown to inhibit TGF-β2-induced ECM expression and alleviate ocular hypertension. However, its underlying molecular mechanisms remain unclear. This study explores the transcriptional, proteomic, and metabolic changes in human TM cells treated with TGF-β2 and salidroside. Human TM cells were treated with TGF-β2 (5 ng/mL) for 48 h, followed by salidroside (30 μM) for 24 h. Multi-omics analyses, including transcriptomics, label-free proteomics, and non-targeted metabolomics, were performed to identify differentially expressed genes (DEGs), proteins (DEPs), and metabolites. The results revealed that TGF-β2 inhibited HTM cell metabolism, affecting pathways like the TCA cycle. Salidroside restores balance by regulating 15 key biomolecules, including MELTF and SLC25A10, through dual-level and post-translation mechanisms. ROC and docking analyses highlight salidroside's role in enhancing metabolic transport and energy activity, with SLC25A10 also linked to RNA processing, showcasing its therapeutic potential. These findings provide valuable insights into POAG pathogenesis and the therapeutic potential of salidroside, offering a foundation for the future development of novel treatment strategies targeting transcriptional, translational, and metabolic dysregulation in POAG.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"256 ","pages":"Article 110386"},"PeriodicalIF":3.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGFβ2 alters segmental outflow and ECM ultrastructure in the trabecular meshwork tgf - β2改变小梁网的节段流出和ECM超微结构

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-10 DOI: 10.1016/j.exer.2025.110377

Timur Mavlyutov, Samer E. Bilal, Justin J. Myrah, Kelsey M. Mathers, Taylor Y. Lee, Colleen M. McDowell

{"title":"TGFβ2 alters segmental outflow and ECM ultrastructure in the trabecular meshwork","authors":"Timur Mavlyutov, Samer E. Bilal, Justin J. Myrah, Kelsey M. Mathers, Taylor Y. Lee, Colleen M. McDowell","doi":"10.1016/j.exer.2025.110377","DOIUrl":"10.1016/j.exer.2025.110377","url":null,"abstract":"<div><div>TGFβ2 is a well-known contributor to extracellular matrix (ECM) changes in the trabecular meshwork (TM). TGFβ2 is increased in the aqueous humor (AH) of primary open angle glaucoma patients and the addition of TGFβ2 to primary human TM cells in culture induces pathogenic changes similar to what is seen in the TM of ocular hypertensive and glaucomatous eyes. Overexpression of a bioactivated form of TGFβ2 using adenovirus 5 (Ad5.TGFβ2) in the TM has previously been described as an inducible mouse model of ocular hypertension and has been utilized for multiple studies to help understand the pathogenies of TGFβ2-induced TM damage and elevated intraocular pressure (IOP). Ad5.TGFβ2 is known to elevate IOP, decrease outflow facility, and increase expression of ECM proteins in the TM. Here, we further analyze the effects of overexpression of TGFβ2 by Ad5 in the TM. We found Ad5.TGFβ2 increases expression of macrophage marker Iba1 and increases expression of ECM proteins fibronectin and collagen 1 compared to Ad5.Null injected controls. In addition, overexpression of TGFβ2 by Ad5 led to a decrease in segmental AH flow regions compared to Ad5.Null control eyes. Ultrastructure analysis of the Ad5.TGFβ2 injected eyes also show significantly more areas occupied by ECM material as well as the development of more smaller giant vacuoles compared to Ad5.Null injected eyes. These data in combination with prior research using Ad5.TGFβ2, establish the use of intraocular injection of Ad5.TGFβ2 as an appropriate mouse model of ocular hypertension to study aqueous humor outflow and its mechanisms.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110377"},"PeriodicalIF":3.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of adenosine triphosphate and coenzyme Q10 on potential hydroxychloroquine-induced retinal damage in rats. 三磷酸腺苷和辅酶Q10对羟氯喹致大鼠视网膜损伤的影响。

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-09 DOI: 10.1016/j.exer.2025.110387

Ahmet Duhan Ozbay , Ahmet Mehmet Somuncu , Ibrahim Cicek , Bulent Yavuzer , Seval Bulut , Gulbaniz Huseynova , Tugba Bal Tastan , Mine Gulaboglu , Halis Suleyman

{"title":"Effects of adenosine triphosphate and coenzyme Q10 on potential hydroxychloroquine-induced retinal damage in rats.","authors":"Ahmet Duhan Ozbay , Ahmet Mehmet Somuncu , Ibrahim Cicek , Bulent Yavuzer , Seval Bulut , Gulbaniz Huseynova , Tugba Bal Tastan , Mine Gulaboglu , Halis Suleyman","doi":"10.1016/j.exer.2025.110387","DOIUrl":"10.1016/j.exer.2025.110387","url":null,"abstract":"<div><div>This study aimed to investigate biochemically and histopathologically the protective effect of adenosine triphosphate (ATP) and coenzyme Q10 (CoQ10) against potential hydroxychloroquine (HCQ)-induced retinal damage in rats. Twenty-four male albino Wistar-type rats were randomly separated into four groups: healthy (HG), receiving HCQ (HQG), receiving ATP + HCQ (AHQ), and receiving CoQ10 + HCQ (CoQHQ). ATP (4 mg/kg, intraperitoneal) was given to the AHQ, and CoQ10 (10 mg/kg, oral) to the CoQHQ. Rats in the HQG, AHQ, and CoQHQ were given HCQ (120 mg/kg, oral) 1 h after administering ATP and CoQ10. Treatments continued once a day for seven days. On the 8th day, the rats were sacrificed with 50 mg/kg sodium thiopental, and the eyes were removed. Malondialdehyde (MDA), total glutathione (tGSH), superoxide dismutase (SOD), and catalase (CAT), and interleukin-6 (IL-6) levels were measured in the retrieved eye tissues and retinal tissues were assessed histopathologically. An increase in MDA and IL-6 levels and a decrease in tGSH, SOD, and CAT levels were detected in the eye tissues of the HQGcompared to the HG. HCQ-induced changes in oxidant and antioxidant levels were significantly suppressed by ATP and CoQ10 treatment. ATP was more successful than CoQ10 in this inhibition. Severe damage was observed in the eye tissues of the HQG group, whereas the damage was mild in the AHQ and moderate in the CoQHQ. Although both ATP and CoQ10 have the potential to be effective in the prevention of HCQ-induced retinal damage through antioxidative activity, ATP appears to be the more preferable treatment approach.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110387"},"PeriodicalIF":3.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen uptake at the ocular surface in diabetic animals is impaired in response to central corneal injury 糖尿病动物眼表氧摄取因角膜中央损伤而受损

IF 3 2区医学

Experimental eye research Pub Date : 2025-04-09 DOI: 10.1016/j.exer.2025.110384

Ana M. Sandoval-Castellanos , Sun Qin , Li Ma , Fernando Ferreira , Brian Reid , Min Zhao

{"title":"Oxygen uptake at the ocular surface in diabetic animals is impaired in response to central corneal injury","authors":"Ana M. Sandoval-Castellanos , Sun Qin , Li Ma , Fernando Ferreira , Brian Reid , Min Zhao","doi":"10.1016/j.exer.2025.110384","DOIUrl":"10.1016/j.exer.2025.110384","url":null,"abstract":"<div><div>Poor wound healing is one of the most devastating complications in late-stage diabetic patients. The avascular cornea does not rely on circulation for its oxygen consumption, uptaking it mainly from the atmosphere. Previous studies demonstrated that oxygen uptake (O<sub>2</sub>U) in diabetic experimental animals and patients is significantly lower than in the non-diabetic condition. Our recent experiments show that upon wounding of the central cornea the O<sub>2</sub>U decreased across the ocular surface, followed by two increases at 6–24 h, and at 72 h, which appeared to be important for proper wound healing. It is however not known whether the two distinct O<sub>2</sub>U increases are maintained in diabetic ocular surface in response to corneal injury. In this study, we used an optic-fiber oxygen micro-sensor to measure O<sub>2</sub>U across the ocular surface of streptozotocin (STZ)- induced diabetic mice and age-matched control mice following injury to the central cornea. We found that the injury causes an immediate and substantial reduction of O<sub>2</sub>U across the ocular surface. O<sub>2</sub>U in non-diabetic corneas increases at 2–6 h post wounding (hpw), decreasing again before the second rise to peak at 72 hpw, especially at the limbus. O<sub>2</sub>U in the diabetic cornea decreases more markedly than that of non-diabetic control. This defective diabetic O<sub>2</sub>U persisted, precluding the two dynamic rises in O<sub>2</sub>U, leading to a failure in recovery. Altogether, our results suggest a previously unknown mechanism of a defective O<sub>2</sub>U response to injury in the diabetic ocular surface, which warrants further research and may lead to new therapeutic paths.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"255 ","pages":"Article 110384"},"PeriodicalIF":3.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0