Experimental eye research最新文献

{"title":"Shikonin treats Aspergillus fumigatus keratitis by activating autophagy and reducing fungal load.","authors":"Xuguang Yang, Fan Cong, Lingwen Gu, Qian Wang, Lina Zhang, Jing Lin, Weichen Liu, Xiaofeng Yao, Cui Li, Guiqiu Zhao","doi":"10.1016/j.exer.2025.110569","DOIUrl":"10.1016/j.exer.2025.110569","url":null,"abstract":"<p><p>The objective of this study was to explore the therapeutic potential of Shikonin (SK) in the treatment of fungal keratitis. Various tests conducted in this study were fluorescein sodium staining, Cell Counting Kit-8 (CCK-8), RT-PCR, Western blot (WB), immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) methods. And the autophagic and phagocytic functions of RAW 264.7 cells were examined using transmission electron microscopy (TEM) and phagocytosis assay. The efficacy of SK in inhibiting Aspergillus fumigatus (A. fumigatus) was confirmed by determining the minimal inhibitory concentration (MIC), crystal violet staining assay, calcofluor white staining (CFW), propidium iodide absorption assay (PI), and fungal adhesion assay. Compared to the group treated with 0.1 % DMSO, the group treated with SK demonstrated a significant decrease in clinical score and the levels of IL-6, TNF-α, and IL-1β. SK also promoted the activity of autophagy proteins LC3B- II/I and Beclin-1 while reducing P62 levels. After treating with SK, there was an increase in autolysosomes in RAW 264.7 cells, accompanied by enhanced phagocytosis of fungi and conidia. Pretreatment with 3-methyladenine (3-MA) counteracted the ability of SK to reduce inflammatory markers. Furthermore, SK inhibited A. fumigatus growth, damaged cell membrane, impaired biofilms, and suppressed conidial adhesion. In conclusion, SK exhibits anti-inflammatory function by activating autophagy and anti-fungal function by reducing fungal load, highlighting its potential treatment effect for fungal keratitis.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110569"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid detection system for fluoroquinolone-resistant Corynebacterium macginleyi.","authors":"Nobuhiro Kato, Masatoshi Haruta, Rikki Arai, Kazunori Sato, Kei Furushima, Miki Okuno, Takeshi Yamamoto, Yoshitoshi Ogura, Shigeo Yoshida","doi":"10.1016/j.exer.2025.110571","DOIUrl":"10.1016/j.exer.2025.110571","url":null,"abstract":"<p><p>We previously reported an increase in high-level fluoroquinolone-resistant Corynebacterium macginleyi isolates from conjunctival swab specimens and investigated the relationship between fluoroquinolone resistance and mutations in the quinolone resistance-determining region (QRDR) of the gene encoding DNA gyrase subunit A (gyrA). The purpose of this study was to develop a rapid system for detecting fluoroquinolone-resistant C. macginleyi using TaqMan single nucleotide polymorphism (SNP) genotyping assays. The study included 49 strains of C. macginleyi, of which 45 strains were isolated from patients awaiting ophthalmic surgery and 4 strains were from patients with presumed keratoconjunctivitis. Real-time polymerase chain reaction (PCR) and SNP genotyping assays were performed by designing TaqMan probes targeting conserved regions in the gyrA gene of C. macginleyi and QRDR sequences associated with fluoroquinolone resistance. Real-time PCR and SNP genotyping assays were completed in approximately 3 h. Genotyping was successful in all 49 strains, and the QRDR nucleotide sequences of the C. macginleyi isolates were compared with the results of the SNP genotyping assays. The SNP genotyping assays effectively discriminated C. macginleyi from other bacterial species and allowed for the determination of fluoroquinolone resistance acquisition in C. macginleyi. TaqMan SNP genotyping assays may serve as a useful tool for the rapid detection of fluoroquinolone-resistant C. macginleyi in conjunctival swab specimens.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110571"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of the PERK pathway attenuates hypoxia-induced apoptosis in a 661W photoreceptor cell model.","authors":"Xingxing Zheng, Cong Han, Keke Ge, Zhi Li, Peirun Wu, Xinran Yu, Yilan Lu, Yi Yang, Wenfang Zhang","doi":"10.1016/j.exer.2025.110667","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110667","url":null,"abstract":"<p><p>High-altitude retinopathy (HAR), characterized by retinal dysfunction under hypobaric hypoxia, remains mechanistically unclear. This study explored hypoxia-induced molecular injury in retinal photoreceptor cells using a hypoxic 661W cell model and identified potential therapeutic targets. Hypoxia triggered endoplasmic reticulum (ER) stress in 661W photoreceptor cells, marked by increased phosphorylation of PERK and eIF2α, upregulation of ATF4, and elevated CHOP expression. Both Salubrinal (Sal) and PERK-targeting siRNAs (PERK-siRNAs) attenuated ER stress via the PERK/eIF2α/ATF4/CHOP pathway, reducing apoptosis and reactive oxygen species (ROS) production by suppressing HIF-1α. Sal further preserved ER morphology, alleviating ultrastructural abnormalities such as ER dilation and mitochondrial swelling observed via transmission electron microscopy. In a HAR mouse model under simulated 5,000 m altitude conditions, Sal improved retinal function, as evidenced by enhanced a- and b-wave amplitudes in electroretinogram (ERG) recordings. These findings suggest that ER stress modulation through PERK pathway inhibition mitigates hypoxia-induced retinal damage, highlighting its potential as a therapeutic strategy for HAR and related retinal disorders.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110667"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of miR-127-5p promotes idiopathic epiretinal membrane development via GLUL-mediated Müller cell activation.","authors":"Jianing Ying, Qian Gui, Shanshan Hua, Hu Li, Tianyu Wang, Danxue He, Jian Zou, Quanyong Yi","doi":"10.1016/j.exer.2025.110561","DOIUrl":"10.1016/j.exer.2025.110561","url":null,"abstract":"<p><p>Idiopathic epiretinal membrane (iERM) represents a fibrocellular proliferation on retinal surface associated with vision loss, with underlying molecular mechanisms that remain undefined. The current work aimed to assess miR-127-5p for its role in the regulation of retinal Müller cell activity in iERM. We analyzed miRNA expression profiles in vitreous samples from iERM patients and controls. The regulatory relationship between miR-127-5p and GLUL (glutamine synthetase) was verified through dual-luciferase reporter assays and molecular studies. The functional effects of the miR-127-5p/GLUL axis manipulation were evaluated in rat retinal Müller cells using proliferation, migration, invasion, and apoptosis assays. miR-127-5p showed significant downregulation in iERM cases. miR-127-5p overexpression in retinal Müller cells suppressed their proliferative, invasive, and migratory capabilities, while inducing apoptotic cell death. GLUL was shown to be directly targeted by miR-127-5p and was upregulated in iERM patients. Knockdown of GLUL phenocopied the impacts of miR-127-5p overexpression, with its overexpression reversing miR-127-5p-associated suppressive effects on Müller cell behavior. These findings suggest miR-127-5p is a key modulator of iERM pathogenesis, functioning through its interaction with GLUL to control retinal Müller cell proliferation and migration. The miR-127-5p/GLUL axis might represent a novel target for iERM treatment.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110561"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zeaxanthin improves myopia by regulating the HIF-1α-glycolysis signaling pathway.","authors":"Xiaoying Wen, Na Yang, Chaohui Gu, Yimeng Zhang, Yuhua Hao","doi":"10.1016/j.exer.2025.110557","DOIUrl":"10.1016/j.exer.2025.110557","url":null,"abstract":"<p><p>Myopia, a highly prevalent refractive error worldwide, occurs when parallel light rays are refracted by the optical system of the eye and converge in front of the retina, leading to blurred vision, with limited effective intervention options available currently. As a natural small-molecule carotenoid, zeaxanthin (Zea) demonstrates antioxidant and anti-inflammatory properties; however, its involvement in myopia is not yet well understood. Accordingly, this research was designed to examine the effects of Zea in treating myopia and the underlying mechanisms involved. In our research, a myopia cell model was created via treating human scleral fibroblasts (HSFs) with hypoxia (1 % O₂), and a myopia animal model was constructed by using form deprivation methods in Wistar rats. In addition, Zea was administered as a therapeutic intervention. Following the intervention, the cell model was evaluated as follows: HSF viability was assessed using the MTT assay; glucose consumption, lactate production, and extracellular acidification rate were measured using commercial assay kits; and the expression levels of myofibroblast markers, hypoxia-inducible factor 1-alpha (HIF-1α), and key proteins involved in glycolysis were analyzed via Western blot. In the in vivo experiments, ophthalmic instruments were used to evaluate anatomical parameters of the rat eye; scleral thickness was measured using hematoxylin-eosin staining; hypoxia levels in the sclera were assessed using immunofluorescence; and myofibroblast markers, HIF-1α, and key proteins involved in glycolysis were assessed via Western blot. The in vitro assays demonstrated that Zea significantly enhanced the viability of HSFs under hypoxic conditions and inhibited their transformation into myofibroblasts. In the in vivo experiments, Zea effectively improved axial length, refractive error, and vitreous chamber depth in rats, while reversing scleral remodeling and hypoxia. Additionally, both in vivo and in vitro experiments showed that Zea notably diminished the vital protein expression levels in the HIF-1α-glycolysis signaling pathway. Zea improves myopia through modulation of the HIF-1α-glycolysis signaling pathway.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110557"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitro Dihydrocapsaicin Attenuates Hyperosmotic Stress-Induced Inflammation in the Corneal Epithelial Cells via SIRT1/Nrf2/ and HO-1 Pathway.","authors":"Jatuporn Ngoenkam, Darawan Pejchang, Toenchit Nuamchit, Uthai Wichai, Sutatip Pongcharoen, Thanet Laorob, Pussadee Paensuwan","doi":"10.1016/j.exer.2025.110680","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110680","url":null,"abstract":"<p><p>A hyperosmotic tear is a central pathogenic factor in dry eye disease (DED) that triggers inflammation, epithelial apoptosis, and structural damage to the corneal epithelium, accompanied by visual disturbances. The transient receptor potential vanilloid 1 (TRPV1) channel, which is highly expressed in corneal epithelial cells, acts as an osmosensor. N-(4-hydroxy-3-nitrobenzyl)-8-methylnonanamide, or nitro dihydrocapsaicin (NDHC), a synthetic TRPV1 agonist, has been investigated for its protective effects against hyperosmotic stress in human corneal epithelial cells (HCECs). Our results demonstrated that an increase in osmotic strength above 480 mOsM markedly caused cell death, as indicated by elevated lactate dehydrogenase (LDH) release and unexpected cell swelling rather than shrinkage, consistent with TRPV1-mediated ionic imbalance. Pretreatment with NDHC mitigated cell swelling, preserved epithelial morphology, and reduced lactate dehydrogenase (LDH) release. Transcriptomic profiling revealed that NDHC significantly reduced the number of differentially expressed genes and partially restored gene signatures, particularly within the TNFα signaling via NF-κB and MTORC1 pathways. Mechanistically, NDHC desensitized TRPV1 activation and activated the SIRT1/Nrf2/HO-1 axis, leading to the suppression of proinflammatory molecules, including IL-6, TNFα and nitric oxide. These effects were abolished by the TRPV1 inhibitor capsazepine, confirming the TRPV1 dependence. In conclusion, NDHC exhibits considerable potential as a dual-therapeutic agent against hyperosmotic stress-induced inflammation in HCECs by modulating TRPV1 activity and attenuating NF-κB-driven inflammation through the enhancement of the SIRT1/Nrf2/HO-1 cascade. These findings suggest that NDHC is a promising therapeutic candidate for alleviating epithelial damage and inflammation in DED.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110680"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel biomarkers and associated immunoregulatory network in pterygium through integrated transcriptome analysis and machine learning approaches.","authors":"Ning Lyu, Jun Xiang, Jiawen Wu, Yidan Fan, Zhaoyuan Lyu, Jiayu Gu, Jingyi Cheng, Jianjiang Xu","doi":"10.1016/j.exer.2025.110570","DOIUrl":"10.1016/j.exer.2025.110570","url":null,"abstract":"<p><p>Pterygium is a common eye disease with unclear pathogenesis. This study aims to identify novel biomarkers for pterygium and explore their mechanisms by RNA sequencing (RNA-seq). Twelve conjunctival and sixteen pterygium samples were collected for RNA-seq. Differential expression analysis, weighted gene co-expression network analysis (WGCNA), and protein-protein interaction (PPI) network analysis were conducted. Multiple machine learning algorithms including XGBoost, Boruta, and LASSO were employed to identify biomarkers. The diagnostic value of biomarkers was evaluated through ROC curve analysis. To explore the functions of biomarkers, gene set enrichment analysis (GSEA), immune infiltration, lncRNA-miRNA-mRNA regulatory network, master regulator analysis (MRA), and transcription factor (TF) mRNA analysis were conducted. qRT-PCR was performed to validate the expression levels of biomarkers. Five novel biomarkers (HSPA8, HBB, ARRB1, IRS1, and FLT4) associated with pterygium were identified. The nomogram model incorporating these biomarkers showed excellent diagnostic performance (AUC = 1). GSEA revealed that these biomarkers were primarily involved in oxidative phosphorylation, cytokine receptor interaction, and chemokine signaling pathways. 168 miRNAs and 1039 lncRNAs related to these biomarkers were predicted in the ceRNA regulatory network. MRA identified Retinoic acid receptor alpha (RARα) as a key TF regulating these biomarkers. Immune infiltration analysis showed significant differences in 22 types of immune cells between pterygium and control groups. The differential expression of five biomarkers was validated using qRT-PCR (all p < 0.05). This study identified five novel pterygium biomarkers (HSPA8, HBB, ARRB1, IRS1, & FLT4) and revealed a complex immunoregulatory network involving immune cells, lncRNA, miRNAs, and TFs (RARα).</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110570"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced pupil light response to red light correlates with cone defect in Rho−/− mice","authors":"Elisa Salamin ,&nbsp;Sylvain V. Crippa ,&nbsp;Catherine Martin ,&nbsp;Aki Kawasaki ,&nbsp;Corinne Kostic","doi":"10.1016/j.exer.2025.110675","DOIUrl":"10.1016/j.exer.2025.110675","url":null,"abstract":"<div><div>This study characterizes retinal degeneration progression in Rhodopsin knockout (<em>Rho</em>^{<em>−/−</em>}<em>)</em> mouse model from 1 to 12 months, comparing molecular markers with functional measures from electroretinogram (ERG) and pupil light response recordings. ERG analyses confirmed the absence of rod activity and a significant decline in photopic responses, indicating cone dysfunction that became undetectable after 3 months. Similarly, immunohistology showed cone alteration at 3 months<em>.</em> Gene expression analysis by RT-QPCR revealed a rapid decrease of rod transducin transcript already from 1 month, while the decline in the cone marker <em>Gnat2</em> was delayed until 6 months. Western blot analysis indicated an earlier reduction in GNAT2 cone protein expression, detectable from 1 month with a significant drop at 2 months. Gene and protein expression of melanopsin, the photopigment of intrinsically photosensitive retinal ganglion cells (ipRGCs), remained stable during rod degeneration, protein level decreasing only at the later ages of 8 and 12 months. Consistent with these findings, pupil response to high-intensity blue stimuli remained stable across age groups, reflecting ipRGCs stability. However, the early dynamics of the pupil response to red and lower blue light stimuli exhibited progressive alterations after 3 months, correlating with cone marker decline. A significant decrease in the derivative area under the curve (dAUC) for low-intensity blue light and low to medium-intensity red stimuli was observed after 3 months, indicating reduced cone contribution with age. This study highlights the correlation between structural and functional cone decline and the maintenance of ipRGCs post-photoreceptor degeneration<strong>,</strong> providing insights for future therapeutic approaches.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"261 ","pages":"Article 110675"},"PeriodicalIF":2.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning method for semi-automated segmentation of optic nerve head tissues in optical coherence tomography images","authors":"Kelly A. Clingo ,&nbsp;Cameron A. Czerpak ,&nbsp;Harry A. Quigley ,&nbsp;Thao D. Nguyen","doi":"10.1016/j.exer.2025.110678","DOIUrl":"10.1016/j.exer.2025.110678","url":null,"abstract":"<div><div>Optical coherence tomography (OCT) enables acquisition of image volumes of the optic nerve head (ONH) in vivo. These image volumes provide structural and biomechanical information about the ONH. The individual tissues in these images must be segmented to obtain tissue-specific information, but doing so manually is time consuming. We present a method which uses a deep learning model to semi-automatically segment the following tissue boundaries of the ONH: the anterior lamina cribrosa surface (ALCS), Bruch's membrane (BM), and choroid-scleral interface (CS). We trained a convolutional neural network (CNN) to predict tissue boundary locations using 46 pre-processed ONH image volumes consisting of 24 radial scans each and their corresponding manual segmentations. When the trained model is presented with a new image, a user is able to accept the model's predictions or drag any misplaced markings to their correct locations. These corrected or accepted markings are used to update the model's predictions for subsequent images. The root mean squared error (RMSE) was used to evaluate the difference between the updated model's predictions and the manually marked segmentations. The RMSE was found to be 8.98 ± 14.74 μm for the BM, 24.26 ± 17.96 μm for the CS, and 49.69 ± 32.08 μm for the ALCS. Updating the model improved predictions for the BM and CS for our test set consisting of image volumes of 6 ONHs, with 24 scans per ONH. A more complete evaluation of the updated model's effect on prediction accuracy could be obtained using a larger test dataset.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"261 ","pages":"Article 110678"},"PeriodicalIF":2.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma N-glycan signature as biomarker for primary open angle glaucoma","authors":"Tsz Kin Ng ,&nbsp;Chunxian Zhang ,&nbsp;Qingping Liu ,&nbsp;Shao-Lang Chen ,&nbsp;Chukai Huang ,&nbsp;Wanli Song ,&nbsp;Chong-Bo Chen ,&nbsp;Yingjie Cao ,&nbsp;Kunliang Qiu ,&nbsp;Li Jia Chen ,&nbsp;Wenzhe Li ,&nbsp;Chi Pui Pang ,&nbsp;Wei Wang ,&nbsp;Mingzhi Zhang","doi":"10.1016/j.exer.2025.110676","DOIUrl":"10.1016/j.exer.2025.110676","url":null,"abstract":"<div><div>Primary open angle glaucoma (POAG) is a major subtype of glaucoma and a leading cause of irreversible visual impairment and blindness worldwide, but with elusive pathogenesis. Here we aimed to delineate the N-glycan profile in the plasma samples of POAG patients and compare to that of the mild senile cataract subjects. Fasting peripheral whole blood was collected from 44 unrelated Han Chinese POAG patients and 39 mild senile cataract subjects. The plasma samples were isolated and subjected to the N-glycan profiling analysis by high throughput fluorophore-assisted capillary gel electrophoreses in an DNA sequencer. In total, 21 major asialo N-glycan peaks were observed in the plasma samples of the POAG patients and cataract subjects through the capillary gel electrophoresis analysis. Relative peak height proportions of Peak III, VIII, and X showed significant differences in the POAG patients as compared to the cataract subjects, and combined Peak III, VIII, and X achieved an AUC of 0.792. Linear correlation analysis revealed a negative correlation of Peak X with mean deviation of visual field in the POAG patients. Notably, sex-specific N-glycan differences were observed. Lectin blotting demonstrated higher level of core fucosylation on the plasma immunoglobulin G of the POAG patients. In summary, this study revealed the N-glycan profiles in the plasma samples of POAG patients. The plasma N-glycan signature can be a potential novel biomarker for POAG.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"261 ","pages":"Article 110676"},"PeriodicalIF":2.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}