Experimental eye research最新文献

Morphological characterization of retinal development from birth to adulthood via retinal thickness assessment in mice: a systematic review.

IF 3 2区医学

Experimental eye research Pub Date : 2025-01-02 DOI: 10.1016/j.exer.2024.110229

Simon Brais-Brunet, Caroline Boudoux, Mathieu Dehaes

{"title":"Morphological characterization of retinal development from birth to adulthood via retinal thickness assessment in mice: a systematic review.","authors":"Simon Brais-Brunet, Caroline Boudoux, Mathieu Dehaes","doi":"10.1016/j.exer.2024.110229","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110229","url":null,"abstract":"The morphology and thickness of the retinal layers are valuable biomarkers for retinal health and development. The retinal layers in mice are similar to those in humans; thus, a mouse is appropriate for studying the retina. The objectives of this systematic review were: (1) to describe normal retinal morphology quantitatively using retinal layer thickness measured from birth to age 6 months in healthy mice; and (2) to describe morphological changes in physiological retinal development over time using the longitudinal (in vivo) and cross-sectional (ex vivo) data from the included studies. A PubMed search was conducted for articles published from to 1980-2024 that included quantitative data. Prior to sexual maturity, an increase in the total retinal and inner plexiform layer thicknesses were observed, with a decrease in the inner nuclear layer thickness. After sexual maturity, an asymptotic decrease in thickness was observed up to age 6 months in all layers; during this period, no significant changes were observed in the outer nuclear layer or nerve fiber layer/ganglion cell layer complex. Potential sources of variability and inconsistency among the studies included differences in imaging modality, animal strain, measurement timing, and retinal segmentation/assignment techniques. These findings highlight the importance of including a control group in experimental designs and providing comparative data for further investigations.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110229"},"PeriodicalIF":3.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative stress mediates retinal damage after corneal alkali burn through the activation of the cGAS/STING pathway.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-28 DOI: 10.1016/j.exer.2024.110228

Keli Mao, Yanqiao Huang, Zheng Liu, Wenjun Sui, Chong Liu, Yujie Li, Jieting Zeng, Xiaobing Qian, Xinqi Ma, Xiaofeng Lin, Bingsheng Lou

{"title":"Oxidative stress mediates retinal damage after corneal alkali burn through the activation of the cGAS/STING pathway.","authors":"Keli Mao, Yanqiao Huang, Zheng Liu, Wenjun Sui, Chong Liu, Yujie Li, Jieting Zeng, Xiaobing Qian, Xinqi Ma, Xiaofeng Lin, Bingsheng Lou","doi":"10.1016/j.exer.2024.110228","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110228","url":null,"abstract":"Retinal damage accounts for irreversible vision loss following ocular alkali burn (OAB), but the underlying mechanisms remain largely unexplored. Herein, using an OAB mouse model, we examined the impact of oxidative stress (OS) in retinal damage and its molecular mechanism. Results revealed that OS in the retina was enhanced soon after alkali injury. Antioxidant therapy with N-acetylcysteine (NAC) preserved the retinal structure, suppressed cell apoptosis and decreased retinal inflammation, confirming the role of OS. Moreover, enhanced OS was linked to mitochondrial dysfunction, mtDNA leakage and initiation of the cytosolic DNA-sensing signaling. The activation of the major DNA sensors cyclic GMP-AMP Synthase (cGas) and cGAS-Stimulator of Interferon Genes (cGAS/STING) pathway was then identified. Notably, inhibiting cGAS/STING signaling with C-176 markedly reduced inflammation and cell apoptosis and ultimately protected the retina against OAB. Overall, our study reveals the vital function of OS in the occurence of OAB-induced retinal damage and the involvement of cGAS/STING activation. Furthermore, our provides preclinical validation of the use of an antioxidant or a STING inhibitor as a potential therapeutic approach to protect the retina after OAB.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110228"},"PeriodicalIF":3.0,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial:In memory of Jerry Lutty.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-28 DOI: 10.1016/j.exer.2024.110226

引用次数: 0

Metabolomic and transcriptomic analysis reveals metabolic-immune interactions in choroid neovascularization.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-26 DOI: 10.1016/j.exer.2024.110227

Yihan Zhang, Siyi Qi, Weiai Shen, Ying Guo, Yu Liang, Qiao Zhuo, Hongyu Kong, Shujie Zhang, Chen Zhao

{"title":"Metabolomic and transcriptomic analysis reveals metabolic-immune interactions in choroid neovascularization.","authors":"Yihan Zhang, Siyi Qi, Weiai Shen, Ying Guo, Yu Liang, Qiao Zhuo, Hongyu Kong, Shujie Zhang, Chen Zhao","doi":"10.1016/j.exer.2024.110227","DOIUrl":"10.1016/j.exer.2024.110227","url":null,"abstract":"Choroid neovascularization (CNV) is a distinct type of age-related macular degeneration (AMD) with a poor prognosis and responsible for the majority of vision loss in the elderly population. The laser-induced CNV model is a well-established animal model frequently used to study CNV. In this study, we performed an integrated analysis of metabolomic and transcriptomic data from CNV samples, utilizing multiple approaches including single-sample gene set enrichment analysis (ssGSEA), correlation analysis, and weighted gene co-expression network analysis (WGCNA), alongside various bioinformatics platforms, to identify key metabolic and immune signatures and to investigate their interplay during angiogenesis. Dominant infiltration of macrophages and monocytes was detected and a positive correlation between dysregulated riboflavin metabolism and angiogenesis pathways was characterized. Hub genes such as ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) and acid phosphatase 5, tartrate resistant (ACP5) emerged as potential central regulators of immune-metabolic crosstalk in CNV. The classification of the immune and metabolic landscape and their critical interactions in CNV models will enhance the understanding of the pathogenesis of neovascular AMD and other neovascular eye diseases, contributing to the development of multi-targeted therapeutic strategies with better efficacy.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110227"},"PeriodicalIF":3.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of effects of 1% atropine in myopia therapy in Guinea pigs.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-25 DOI: 10.1016/j.exer.2024.110224

Chen Chu, Luyao Ye, Qingqing Chi, Jiangnan He, Jianfeng Zhu

{"title":"Proteomic analysis of effects of 1% atropine in myopia therapy in Guinea pigs.","authors":"Chen Chu, Luyao Ye, Qingqing Chi, Jiangnan He, Jianfeng Zhu","doi":"10.1016/j.exer.2024.110224","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110224","url":null,"abstract":"Myopia is a significant global public health issue. Key interventions for managing myopia include atropine treatment, optical correction, and surgical methods. This study focused on evaluating alterations in retinal protein expression after atropine therapy for myopia. Guinea pigs were randomly divided into four groups: control (CON), monocular form-deprivation myopia (FDM), FDM with 2-week atropine treatment (FDM + ATR), and atropine-only treatment (ATR). After two weeks of FDM induction, the FDM group showed significant differences in refractive error and increased axial lengths. In comparing the retinas of myopic and normal eyes, 30 proteins were found to have increased expression, while 8 proteins showed decreased expression. Atropine-treated retinas exhibited 73 proteins with increased expression and 29 proteins with decreased expression compared to the normal eyes. A total of 11 regulated proteins overlapped between the FDM + ATR vs FDM and FDM vs CON groups. IPA analysis indicates significant alterations in amino acid metabolism, energy production, post-translational modification, small molecule biochemistry, and free radical scavenging. Our study identifies retinal protein changes in myopic guinea pigs and in guinea pigs treated with atropine after myopia. These proteins could serve as potential targets for atropine treatment of myopia.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"251 ","pages":"110224"},"PeriodicalIF":3.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring ocular disorders in Parkinson's disease: A comprehensive review and future perspectives.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-24 DOI: 10.1016/j.exer.2024.110225

Minal Thacker, Ka Ying Wong, Liping Zhou, Juewen Liu, Man-Sau Wong

引用次数: 0

Photobiomodulation inhibits retinal degeneration in diabetic mice through modulation of stem cell mobilization and gene expression.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-22 DOI: 10.1016/j.exer.2024.110218

Jingyan Ge, Yinan Zhang, Ling Han, Liangliang Zhao, Hongwei Zhao, Dan Qiao, Yan Cheng

{"title":"Photobiomodulation inhibits retinal degeneration in diabetic mice through modulation of stem cell mobilization and gene expression.","authors":"Jingyan Ge, Yinan Zhang, Ling Han, Liangliang Zhao, Hongwei Zhao, Dan Qiao, Yan Cheng","doi":"10.1016/j.exer.2024.110218","DOIUrl":"10.1016/j.exer.2024.110218","url":null,"abstract":"The number of people suffering from type 2 diabetes (DM2) is increasing and over 30 percent of DM2 patients will develop diabetic retinopathy (DR). Available therapeutic approaches for DR have their limitations. It is of great significance to search for other effective alternate therapeutic approaches. The present study aimed to explore the beneficial effects of photobiomodulation (PBM) on the diabetic retinopathy and underlying mechanisms. Streptozotocin was administered to male mice to establish diabetic model. The mice in the diabetic group (DM) received no treatment, and the mice in DM + PBM group received LED illumination (wavelength 670 nm) once a day for 20 consecutive weeks. Retinal vessel degenerate changes, the expression levels of E-Cadherin, N-Cadherin and the mRNA levels of c-kit, CXCR4, MYPT1, SCF, SDF1-α in retina, the levels of SDF-1α and SCF in the peripheral blood and the number of LSK cells expressing c-kit and sca-1 were determined. PBM could significantly inhibit the degenerative change of diabetic retinal vessels, decrease the expression levels of E-Cadherin and N-Cadherin and the mRNA levels of c-kit, CXCR4, MYPT1, SCF, SDF1-α and increase VEGF mRNA levels in retina. PBM could also increase the levels of SDF-1α and SCF in the peripheral blood and the number of LSK cells expressing c-kit and sca-1 in diabetic mice. PBM at 4 min/day for 20 consecutive weeks significantly inhibit the degenerative change of diabetic retinal vessels, and PBM is likely to produce its beneficial effects on the retina through promoting the migration of bone marrow stem cells to circulation and diabetic retinal tissue. The present study provides a new therapeutic direction and experimental foundation for the treatment of diabetic retinopathy.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110218"},"PeriodicalIF":3.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How crosstalk between mitochondria, lysosomes, and other organelles can prevent or promote dry age-related macular degeneration.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-22 DOI: 10.1016/j.exer.2024.110219

Aparna Lakkaraju, Patricia Boya, Marie Csete, Deborah A Ferrington, James B Hurley, Alfredo A Sadun, Peng Shang, Ruchi Sharma, Debasish Sinha, Marius Ueffing, Susan E Brockerhoff

{"title":"How crosstalk between mitochondria, lysosomes, and other organelles can prevent or promote dry age-related macular degeneration.","authors":"Aparna Lakkaraju, Patricia Boya, Marie Csete, Deborah A Ferrington, James B Hurley, Alfredo A Sadun, Peng Shang, Ruchi Sharma, Debasish Sinha, Marius Ueffing, Susan E Brockerhoff","doi":"10.1016/j.exer.2024.110219","DOIUrl":"10.1016/j.exer.2024.110219","url":null,"abstract":"Organelles such as mitochondria, lysosomes, peroxisomes, and the endoplasmic reticulum form highly dynamic cellular networks and exchange information through sites of physical contact. While each organelle performs unique functions, this inter-organelle crosstalk helps maintain cell homeostasis. Age-related macular degeneration (AMD) is a devastating blinding disease strongly associated with mitochondrial dysfunction, oxidative stress, and decreased clearance of cellular debris in the retinal pigment epithelium (RPE). However, how these occur, and how they relate to organelle function both with the RPE and potentially the photoreceptors are fundamental, unresolved questions in AMD biology. Here, we report the discussions of the \"Mitochondria, Lysosomes, and other Organelle Interactions\" task group of the 2024 Ryan Initiative for Macular Research (RIMR). Our group focused on understanding the interplay between cellular organelles in maintaining homeostasis in the RPE and photoreceptors, how this could be derailed to promote AMD, and identifying where these pathways could potentially be targeted therapeutically.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110219"},"PeriodicalIF":3.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of different exercise modalities on oxidative stress in animal models of high intraocular pressure and diabetes.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110216

Sabrina Nau da Silva Piazza, Paula Bortoluzzi Canteiro, Natalia Dos Santos Tramontin, Giulia Strapazzon, Vanessa de Moraes Andrade, Alexandre Pastoris Muller

{"title":"Protective effects of different exercise modalities on oxidative stress in animal models of high intraocular pressure and diabetes.","authors":"Sabrina Nau da Silva Piazza, Paula Bortoluzzi Canteiro, Natalia Dos Santos Tramontin, Giulia Strapazzon, Vanessa de Moraes Andrade, Alexandre Pastoris Muller","doi":"10.1016/j.exer.2024.110216","DOIUrl":"10.1016/j.exer.2024.110216","url":null,"abstract":"High intraocular pressure (HIOP) and high glucose levels are associated with oxidative stress. Although physical exercise protects against oxidative damage, its specific impact on eye health remains unclear. Thus, this study aimed to assess the impact of physical exercise on the oxidative status of whole eyes in male Swiss mice subjected to HIOP model and cafeteria diet (CD). In experiment one, mice were divided into sedentary, aerobic, and strength (four-week physical exercise) groups and subjected to an HIOP/ischemia model. In experiment two, mice were submitted to CD and voluntary physical exercise for 18 weeks, according to the following groups: sedentary control, sedentary CD, exercise control, and exercise CD. Experiment one revealed elevated 2',7'-dichlorodihydrofluorescein (DCFH) levels in aerobic group, which decreased in all groups after ischemia. Nitrite levels were decreased on strength than in sedentary group. The superoxide dismutase (SOD) activity did not change in all treatments. Although catalase (CAT) activity increased in aerobic and strength groups, and after ischemia in all groups. In experiment two, the sedentary CD group presented higher body weight than the other groups. DCFH levels were increased in the exercise control and reduced in the exercise CD compared with the other groups. CAT activity and sulfhydryl groups were decreased, while protein carbonylation was increased in the sedentary CD group compared with the other groups. Thus, these results suggested that physical exercise promoted antioxidant effects on eyes exposed to an HIOP model and CD.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110216"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The vectors went in two-by-two: Transduction efficiency and tolerability of dual and triple rAAV vector delivery following intravitreal injection for genome-editing applications.

IF 3 2区医学

Experimental eye research Pub Date : 2024-12-20 DOI: 10.1016/j.exer.2024.110223

Rachel L Fehrman, Kristina J Chern, Kyle P Stoltz, Daniel M Lipinski

{"title":"The vectors went in two-by-two: Transduction efficiency and tolerability of dual and triple rAAV vector delivery following intravitreal injection for genome-editing applications.","authors":"Rachel L Fehrman, Kristina J Chern, Kyle P Stoltz, Daniel M Lipinski","doi":"10.1016/j.exer.2024.110223","DOIUrl":"https://doi.org/10.1016/j.exer.2024.110223","url":null,"abstract":"Genome or prime editing has become a promising tool for the treatment of hereditary disorders affecting the inner retina, such as dominant optic neuropathies. In vivo delivery of gene editors, such as Cas9, is typically achieved using recombinant adeno-associated virus (rAAV) vectors, which have a broad range of cellular tropisms and are well tolerated following intravitreal administration. Owing to the large size of gene editing constructs and the limited carrying capacity of rAAV (<5.1kb) it is unfortunately usually necessary to split therapeutic transgene cassettes across multiple co-administered vector genomes. While the efficiency with which multiple vector genomes recombine following cellular entry has been studied extensively, another potentially limiting factor is the likelihood of target cells (e.g. retinal ganglion cells) receiving two or more vectors containing genomes that correspond to the full-length expression cassette when recombined. In this study we examine the efficiency with which two or more vector genomes transduce various retinal cell types following intravitreal administration. rAAV2/2[MAX] vectors expressing individual fluorescent reporters (GFP, BFP or mCherry) were co-injected intravitreally singly or in combination (dual or triple), allowing the extent of co-transduction to be assessed through multimodal in vivo imaging, electroretinography, flow cytometry and post-mortem histology. We find that intravitreal co-administration of vectors containing multiple genomes is well tolerated - with no observed alterations in retinal thickness or ERG amplitudes - but that co-transduction efficiency decreases significantly with increasing genome number. As such co-transduction of multiple vectors may be a major bottleneck limiting gene editing of inherited disorders affecting the inner retina.","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110223"},"PeriodicalIF":3.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0