Experimental eye research最新文献

{"title":"Integration of single-cell and bulk transcriptomics reveals β-hydroxybutyrylation-related signatures in primary open-angle glaucoma.","authors":"Qing Dai, Sijie Zhao, Juan Li, Ning Li, Aiqin Wang, Ziqing Gao, Yuchen Fan","doi":"10.1016/j.exer.2025.110272","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110272","url":null,"abstract":"<p><p>The pathophysiology of primary open-angle glaucoma (POAG), the most prevalent glaucoma type, is poorly understood. Although it is well known that epigenetic factors affect the progression of POAG, the impact of β-hydroxybutyrylation (Kbhb) on POAG remains unknown. Based on POAG-related datasets (GSE27276, GSE4316, and GSE231749) retrieved from the Gene Expression Omnibus (GEO) database, four biomarkers (FABP5, GLS, PDLIM1, and TAGLN) with a diagnostic value for POAG were identified by combining differential expression analysis, machine learning algorithms, and receiver operating characteristic (ROC) analysis. Immune infiltration analysis demonstrated significant differences in the infiltration abundances of 10 immune cells between POAG and controls, including regulatory T cells, monocytes, and macrophages, with notable positive correlations between TAGLN expression and these immune cells. Subsequently, single-cell analysis revealed that GLS, PDLIM1, and TAGLN were higher expressed in chondrocytes, smooth muscle cells, and endothelial cells. In addition, in vitro cellular experiments and animal models revealed that the TAGLN expression trend was consistent with the data from GSE27276 and GSE4316. In conclusion, TAGLN may play an important role in understanding of the molecular mechanisms of POAG and exploration of therapeutic targets.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110272"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinoids in lipofuscin granules from retinal pigment epithelium as biomarkers of the damaging effect of ionizing radiation.","authors":"Tatiana B Feldman, Marina A Yakovleva, Mikhail A Ostrovsky","doi":"10.1016/j.exer.2025.110270","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110270","url":null,"abstract":"<p><p>Lipofuscin granules accumulate in the retinal pigment epithelium with age, especially in patients with visual diseases, including progressive age-related macular degeneration. Retinoids (bisretinoids and their oxidation products) are major sources of lipofuscin granule fluorescence. The aim of this work was to analyze the radiation-mediated oxidation of retinoids in lipofuscin granules obtained from the human cadaver eye retinal pigment epithelium. Fluorescent and chromatographic analyses of retinoids were performed before and after irradiation of lipofuscin granules with accelerated protons. The fluorescent properties of chloroform extracts from irradiated lipofuscin granules exhibited an increase in fluorescence intensity in the short-wavelength region of 555 nm. This change is associated with an increase in the quantity of retinoid oxidation cytotoxic products after accelerated proton exposure. The radiation-induced oxidation of retinoids caused a noticeable change in the fluorescent properties of retinoids allows us to consider this phenomenon as a potential opportunity for non-invasively assessment of the degree of radiation exposure and its relative biological effect in humans. Thus, this research proposes a new strategy for assessing the extent of radiation exposure to humans, which evaluates the effects of ionizing radiation on human eye tissues. This approach is based on the principles of the modern non-invasive method of fundus autofluorescence used in ophthalmology for the diagnosis of the retina and retinal pigment epithelium degenerative diseases.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110270"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N6-methyladenosine (m6A) modification regulates HSPA1A and HSPA1B expression in Müller cells under high glucose stress","authors":"Hong Yang ,&nbsp;Jini Qiu ,&nbsp;Xinhan Cui ,&nbsp;Xueling Zhang ,&nbsp;Rongmei Zhou ,&nbsp;Jianjiang Xu ,&nbsp;Ruiping Gu ,&nbsp;Kun Shan","doi":"10.1016/j.exer.2025.110275","DOIUrl":"10.1016/j.exer.2025.110275","url":null,"abstract":"<div><div>Müller cells (MCs) represent the major glial cells that are responsible for maintaining retinal homeostasis. In diabetic retinopathy, Müller cell activation occurs in the initial stages, playing a role in many pathological processes, such as neovascularization, neuronal dysfunction, and inflammatory retinal environment. As the most common RNA modification in eukaryotes, <em>N</em>⁶-methyladenosine (m⁶A) exerts dynamic and reversible control over cellular functions in the context of high glucose (HG) stress. Here, we performed combined m⁶A and RNA sequencing to elucidate the landscape of m⁶A modification in MCs under HG environmental stimuli. The potential functions of aberrant m⁶A peaks and differentially expressed genes were analyzed using bioinformatics analysis. Our findings indicate that m⁶A modification may regulate the expression of heat shock proteins (HSPs) 70 isoforms HSPA1A and HSPA1B, which are stress-inducible chaperones critical for cell survival under adverse conditions, including hyperglycemia. Modulating m⁶A modification may regulate critical gene expression and cellular functions of MCs under HG stress.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"252 ","pages":"Article 110275"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pigment epithelium-derived factor (PEDF) Promotes Survival and Contraction of Myoepithelial Cells in Lacrimal Gland.","authors":"Yilan Yin, Colin James Barnstable, Xiaomin Zhang, Xiaorong Li, Shaozhen Zhao, Joyce Tombran-Tink","doi":"10.1016/j.exer.2025.110269","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110269","url":null,"abstract":"<p><p>PEDF is critical for general health of the ocular surface. In order to study mechanisms of PEDF's action in lacrimal gland (LG) secretion, these visual structures were studied in a PEDF deficient (Pedf^-/-) mouse model using biochemical, histochemical, and morphometric analyses. In Pedf^-/- animals there were several ocular surface and LG disturbances not seen in controls. Notably, changes in body and LG weight, corneal sensitivity, tear film, ocular surface damage, and size of acini comprising the LG were evident. Survival of myoepithelial cells (MECs) surrounding the acini showed a PEDF survival dependence as there were significant reduction in MEC-specific P63 cells and Bcl2 expression levels, and increased TUNEL positive cells in PEDF deficient mice. Expression levels of the major contractile MEC proteins, α-SMA, calponin, and keratin 14, were also reduced with PEDF gene deletion and MECs contraction apparatus impaired, since oxytocin significantly reduced acinar area in controls but had no effect in PEDF deficient LGs, although the oxytocin receptor (OXTR) was expressed in both PEDF genotypes. These findings suggest that PEDF is essential to MECs survival and contractile function, and tear homeostasis on the ocular surface. Treatment with PEDF is likely to alleviate ocular-related conditions in diseases associated with dry eye as well as promote healthy MEC cell function in other secretory glands of the body.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110269"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant adeno-associated virus with anti-tumor necrosis factor-alpha in an experimental autoimmune uveitis model.","authors":"Baiyi Li, Chuan Zhao, Shengjie Guo, Xueru Li, Hui Zhang, Yanan Duan, Mi Zhang, Qingqin Tao, Peiran Zhou, Xiaorong Li, Xiaomin Zhang","doi":"10.1016/j.exer.2025.110273","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110273","url":null,"abstract":"<p><p>Uveitis treatment is associated with side effects and inconsistent outcomes. Existing treatments often fail to provide targeted and sustained relief; thus, novel therapeutic approaches are needed. Among these, gene therapy using adeno-associated virus (AAV) vectors target specific retinal cells, show low immunogenicity, and demonstrate sustained gene expression, making it a potential advancement in uveitis treatment. Therefore, we utilized a AAV2 system encapsulating encoded anti-tumor necrosis factor-alpha (TNF-α) antibody to assess its efficacy in the treatment of experimental autoimmune uveitis (EAU) in mice. Compared with the AAV2-GFP group, AAV2-ADA-injected mice showed significantly reduced clinical, OCT, and histopathological scores in EAU with lower percentages of Th1 and Th17 cells in the eyes and higher percentages of Treg cells in the draining lymph nodes (LN). This study demonstrated the safety and effects of AAV2-ADA in EAU treatment, providing a promising therapeutic strategy for uveitis.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110273"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in vascular resistance with intraocular pressure and damage severity in experimental glaucoma","authors":"Stuart K. Gardiner,&nbsp;Grant Cull,&nbsp;Brad Fortune","doi":"10.1016/j.exer.2025.110271","DOIUrl":"10.1016/j.exer.2025.110271","url":null,"abstract":"<div><div>There is evidence of changes in retinal hemodynamics in both experimental glaucoma and human disease. A major potential confound is that intraocular pressure (IOP) may also be directly affecting vascular resistance and/or the vasodilatory capacity of vessels in the optic nerve head and retina. This is particularly problematic in experimental glaucoma, where chronic IOP elevation is maintained, without the IOP-reducing medications typically used by patients involved in human studies. However, those animal studies remain invaluable, due to the possibility of extensive baseline testing in the knowledge that no glaucomatous loss has commenced, and due to the degree of control that is possible over parameters such as medication regimens. In this study, we aim to assess the impact of chronic IOP elevation on vascular resistance parameters, and separate it from the impact of glaucomatous damage severity. Longitudinal measurements were made using laser speckle flowgraphy before and after unilateral IOP elevation in 31 non-human primates. The pulsatile waveform was extracted and used to calculate the pulsatility index (maximum minus minimum, as a proportion of the mean) and resistivity index (maximum flow minus minimum flow, as a proportion of the maximum), in both the major vessels and the other tissue within the optic nerve head, for an average of 18 time points per animal. The vascular resistance indices increased with IOP at both locations, both in the full dataset, and in the subset of data points restricted to the visit at which IOP first exceeded 30 mmHg until the resistance index reached its maximum for that eye (all p &lt; 0.001). After adjusting for the influence of IOP using coefficients from linear mixed effects models, the resistance indices exhibited non-monotonic relations with damage severity, first increasing from baseline, but then decreasing back to or beyond the normal range in eyes with more severe damage. Further studies are needed to accurately characterize the location and timing of these changes during the course of glaucomatous damage, which would help identify the pathophysiologic processes that are underway at different stages of the disease.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"252 ","pages":"Article 110271"},"PeriodicalIF":3.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein Modulates Endoplasmic Reticulum Stress by Activating SIRT3 to Attenuate the Dysfunction of Retinal Microvascular Endothelial Cells under High Glucose Conditions.","authors":"Si-Min Wang, Yang Xia","doi":"10.1016/j.exer.2025.110250","DOIUrl":"https://doi.org/10.1016/j.exer.2025.110250","url":null,"abstract":"<p><p>High glucose-induced alterations in the retinal microvasculature are major contributors to vision loss and deterioration. Baicalein, known for its protective effect on blood vessels and endothelial cells, is a potential therapeutic agent for diabetes-induced retinal dysfunction. This study aims to investigate the impact and mechanism of baicalein on high glucose-induced dysfunction in retinal microvascular endothelial cells (RMECs). Human RMECs (hRMECs) were exposed to a high glucose condition (30 mM). The effect of various concentrations of baicalein on cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Baicalein's effects on sirtuin 3 (SIRT3) expression in hRMECs were evaluated via western blot and quantitative real-time polymerase chain reaction. Additionally, the regulatory role of baicalein was examined by knocking down SIRT3. Cell permeability and migration were assessed using the Transwell assay, and tube formation was evaluated by tube formation assay. Moreover, western blot analysis was employed to investigate protein expression related to endoplasmic reticulum stress (ERS). Baicalein markedly inhibited the increase in the viability, permeability, tube formation and migration of hRMECs induced by high glucose. Moreover, it significantly reduced the intracellular ERS levels in high glucose-induced hRMECs. Notably, SIRT3 knockdown reversed the inhibition of baicalein on hRMECs. In summary, baicalein mitigates high glucose-mediated ERS by up-regulating SIRT3 expression, thereby maintaining the normal function of RMECs.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110250"},"PeriodicalIF":3.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstruction of rabbit corneal epithelium using adipose and / or bone marrow stem cells","authors":"Taghreed A. Hassan ,&nbsp;Yara S. Abouelela ,&nbsp;Zainab Sabry Othman Ahmed ,&nbsp;Marwa A. Ibrahim ,&nbsp;Hamdy Rizk ,&nbsp;Ayman Tolba","doi":"10.1016/j.exer.2024.110203","DOIUrl":"10.1016/j.exer.2024.110203","url":null,"abstract":"<div><div>One of the main causes of corneal blindness is corneal alkali burn, which can also result in serious side effects such as limbal stem cell deficit, corneal perforation, and permanent epithelial abnormalities. This study set out to investigate the therapeutic potential of ADMSCs and BMMSCs for the reconstruction of the corneal surface after chemical alkali burn. Twelve adult rabbits were divided equally into four groups. Each rabbit in the other groups had a chemical alkali burn applied to their right eye using 6 mm-wide NaoH soaked filter paper, while the negative control group had no intervention. All groups except negative control group received topical and subconjunctival injections. Group I (Negative control) received no therapy, whereas Group II received an injection of phosphate-buffered saline as the positive control. Group III received 1 mL of ADMSCs, while Group IV received 1 mL of BMMSCs. After 4 weeks, the corneal tissue underwent morphological, histological, immunohistochemical examination and gene expression. The ocular tissue underwent histopathological examination revealed re-epithelialization and nearly normal architecture in the BMMSC-treated group. The injured cornea treated with ADMSCs showed partial repair of the anterior epithelium, in addition to inflammatory cells infiltration. An immunohistochemical analysis revealed that, compared to ADMSCs and positive control groups, the majority of the stromal cells in the cornea treated by BMMSCs exhibited robust positive expression of vimentin and Ki67. BMMSCs exhibited considerably higher levels of gene expression for corneal indicators, such as keratin 12 and connexin 43, in comparison to other groups. In treating a corneal chemical burn, this study shows that MSCs produced from bone marrow and adipose tissue effectively reduce tissue inflammation, enhance corneal tissue repair, and stimulate cell renewal, with BMMSCs showing better outcomes.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"251 ","pages":"Article 110203"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-GlcNAcylation promotes astroglial-mesenchymal transition via the connexin43 pathway under high-glucose conditions","authors":"Guodong Liu ,&nbsp;Hui Li ,&nbsp;Le Feng ,&nbsp;Min Li ,&nbsp;Peng Gao ,&nbsp;Fang Wang","doi":"10.1016/j.exer.2024.110206","DOIUrl":"10.1016/j.exer.2024.110206","url":null,"abstract":"<div><div>This study aimed to investigate the effects of O-linked N-acetylglucosamine modification (O-GlcNAcylation) on astroglial-mesenchymal transition through connexin43 (Cx43) pathway under high-glucose conditions. The primary rat astrocytes were cultured under normal and high-glucose conditions, and level of GFAP, α-SMA and Cx43 was investigated. To explore the influence of O-GlcNAcylation on astroglial-mesenchymal transition, Thiamet G treatment was employed to enhance O-GlcNAcylation, while Alloxan was used to decrease it. Cx43 knockdown was acquired through lentivirus constructs to explore its role in astrocyte transition. The levels of GFAP and α-SMA expressions were examined, while astrocyte proliferation was evaluated using the CCK-8 assay, and migration was assessed through wound healing assays. The results showed that primary rat astrocytes were identified by GFAP antibody staining. Under high-glucose conditions, the levels of GFAP, α-SMA, and Cx43 increased, as confirmed by Western blot and immunofluorescence. O-GlcNAcylation augmentation induced by Thiamet G treatment significantly increased the expression of GFAP, α-SMA, and Cx43 compared to both normal and high-glucose conditions. Conversely, the inhibition of O-GlcNAcylation reversed the high-glucose-induced increase in GFAP and α-SMA. Cx43 knockout led to the downregulation of GFAP and α-SMA compared to high-glucose and O-GlcNAcylation-augmented conditions. Additionally, levels of O-GlcNAcylation and VEGF were reduced in Cx43 knockout group. Consistently, CCK8 and wound healing assays demonstrated that Cx43 knockout could inhibit astrocyte proliferation and migration compared to the high-glucose and O-GlcNAcylation augmented groups. These findings demonstrate that astroglial-mesenchymal transition occurs under high-glucose conditions, and can be promoted by O-GlcNAcylation augmentation, but suppressed by Cx43 knockout. The study underscores the significant role of Cx43 in this transition and its potential involvement in diabetic complications.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"251 ","pages":"Article 110206"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characterization of retinal development from birth to adulthood via retinal thickness assessment in mice: A systematic review","authors":"Simon Brais-Brunet ,&nbsp;Caroline Boudoux ,&nbsp;Mathieu Dehaes","doi":"10.1016/j.exer.2024.110229","DOIUrl":"10.1016/j.exer.2024.110229","url":null,"abstract":"<div><div>The morphology and thickness of the retinal layers are valuable biomarkers for retinal health and development. The retinal layers in mice are similar to those in humans; thus, a mouse is appropriate for studying the retina. The objectives of this systematic review were: (1) to describe normal retinal morphology quantitatively using retinal layer thickness measured from birth to age 6 months in healthy mice; and (2) to describe morphological changes in physiological retinal development over time using the longitudinal (<em>in vivo</em>) and cross-sectional (<em>ex vivo</em>) data from the included studies. A PubMed search was conducted for articles published from to 1980–2024 that included quantitative data. Prior to sexual maturity, an increase in the total retinal and inner plexiform layer thicknesses were observed, with a decrease in the inner nuclear layer thickness. After sexual maturity, an asymptotic decrease in thickness was observed up to age 6 months in all layers; during this period, no significant changes were observed in the outer nuclear layer or nerve fiber layer/ganglion cell layer complex. Potential sources of variability and inconsistency among the studies included differences in imaging modality, animal strain, measurement timing, and retinal segmentation/assignment techniques. These findings highlight the importance of including a control group in experimental designs and providing comparative data for further investigations.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"251 ","pages":"Article 110229"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}