Chen Pengjie, Sun Yiming, Wu Han, Miao Qi, Xu Mingyu, Wang Jiawei, Xu Peifang, Ye Juan
{"title":"多组学分析揭示睑板腺衰老的脂质代谢特征和调控网络。","authors":"Chen Pengjie, Sun Yiming, Wu Han, Miao Qi, Xu Mingyu, Wang Jiawei, Xu Peifang, Ye Juan","doi":"10.1016/j.exer.2025.110457","DOIUrl":null,"url":null,"abstract":"<div><div>Aging is a significant risk factor for ocular surface diseases like meibomian gland dysfunction (MGD), which compromises tear film stability, leading to discomforts, visual impairment, and ocular structural damage. This study aims to elucidate the age-related changes in lipid metabolism and the regulation networks, providing insights into early pathogenic mechanisms and identifying potential molecular targets for preventing age-driven pathology in the MGs. We analyzed MGs from young (2 months) and aged (12 months) C57BL/6 mice using a multi-omics approach, transcriptomic analysis and lipidomic analysis. Our findings revealed shifts in lipid composition in aged MGs, especially with reduced phospholipid levels and elevated triglyceride (TG) levels. Differentially expressed genes (DEGs) in lipid metabolism were also identified, including glycerolipid, glycerophospholipid, and sphingolipid metabolism, forming a complex regulatory network of lipid metabolism. Some critical DEGs were validated by qPCR, confirming the upregulation of Akr1b8 (glycerolipid metabolism) and the downregulation of Mboat2 (glycerophospholipid metabolism), Degs2, and Sptlc3 (sphingolipid metabolism) in aged MGs. These findings highlighted lipid metabolism dysregulation as a key factor in age-related MGD, offering potential targets for future research to mitigate this condition and preserve ocular health.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110457"},"PeriodicalIF":3.0000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multi-omics analysis reveals lipid metabolism profiles and regulatory networks in meibomian glands aging\",\"authors\":\"Chen Pengjie, Sun Yiming, Wu Han, Miao Qi, Xu Mingyu, Wang Jiawei, Xu Peifang, Ye Juan\",\"doi\":\"10.1016/j.exer.2025.110457\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Aging is a significant risk factor for ocular surface diseases like meibomian gland dysfunction (MGD), which compromises tear film stability, leading to discomforts, visual impairment, and ocular structural damage. This study aims to elucidate the age-related changes in lipid metabolism and the regulation networks, providing insights into early pathogenic mechanisms and identifying potential molecular targets for preventing age-driven pathology in the MGs. We analyzed MGs from young (2 months) and aged (12 months) C57BL/6 mice using a multi-omics approach, transcriptomic analysis and lipidomic analysis. Our findings revealed shifts in lipid composition in aged MGs, especially with reduced phospholipid levels and elevated triglyceride (TG) levels. Differentially expressed genes (DEGs) in lipid metabolism were also identified, including glycerolipid, glycerophospholipid, and sphingolipid metabolism, forming a complex regulatory network of lipid metabolism. Some critical DEGs were validated by qPCR, confirming the upregulation of Akr1b8 (glycerolipid metabolism) and the downregulation of Mboat2 (glycerophospholipid metabolism), Degs2, and Sptlc3 (sphingolipid metabolism) in aged MGs. These findings highlighted lipid metabolism dysregulation as a key factor in age-related MGD, offering potential targets for future research to mitigate this condition and preserve ocular health.</div></div>\",\"PeriodicalId\":12177,\"journal\":{\"name\":\"Experimental eye research\",\"volume\":\"258 \",\"pages\":\"Article 110457\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental eye research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014483525002283\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OPHTHALMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental eye research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014483525002283","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
Multi-omics analysis reveals lipid metabolism profiles and regulatory networks in meibomian glands aging
Aging is a significant risk factor for ocular surface diseases like meibomian gland dysfunction (MGD), which compromises tear film stability, leading to discomforts, visual impairment, and ocular structural damage. This study aims to elucidate the age-related changes in lipid metabolism and the regulation networks, providing insights into early pathogenic mechanisms and identifying potential molecular targets for preventing age-driven pathology in the MGs. We analyzed MGs from young (2 months) and aged (12 months) C57BL/6 mice using a multi-omics approach, transcriptomic analysis and lipidomic analysis. Our findings revealed shifts in lipid composition in aged MGs, especially with reduced phospholipid levels and elevated triglyceride (TG) levels. Differentially expressed genes (DEGs) in lipid metabolism were also identified, including glycerolipid, glycerophospholipid, and sphingolipid metabolism, forming a complex regulatory network of lipid metabolism. Some critical DEGs were validated by qPCR, confirming the upregulation of Akr1b8 (glycerolipid metabolism) and the downregulation of Mboat2 (glycerophospholipid metabolism), Degs2, and Sptlc3 (sphingolipid metabolism) in aged MGs. These findings highlighted lipid metabolism dysregulation as a key factor in age-related MGD, offering potential targets for future research to mitigate this condition and preserve ocular health.
期刊介绍:
The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.