FEBS Letters最新文献_第5页

Structural diversity of pyruvate dehydrogenase complexes. 丙酮酸脱氢酶复合物的结构多样性。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-13 DOI: 10.1002/1873-3468.70140

Sarah N Bothe, Rafal Zdanowicz

{"title":"Structural diversity of pyruvate dehydrogenase complexes.","authors":"Sarah N Bothe, Rafal Zdanowicz","doi":"10.1002/1873-3468.70140","DOIUrl":"https://doi.org/10.1002/1873-3468.70140","url":null,"abstract":"The pyruvate dehydrogenase complex (PDHc) is a crucial metabolic enzyme complex found in all aerobic organisms. It catalyzes the conversion of pyruvate, the product of glycolysis, into acetyl-CoA, a key substrate for the citric acid cycle and fatty acid synthesis. This multienzyme complex uses multiple cosubstrates and tethered reaction intermediates to efficiently channel substrates through its catalytic steps. With a total size of 5-12 MDa, PDHc is among the largest biomolecular assemblies. It consists of three enzymatic components acting sequentially: E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase). In eukaryotes, an additional E3-binding protein (E3BP) recruits E3 to the complex. E2 (and E3BP) subunits form the structural core, typically exhibiting octahedral or icosahedral symmetry, while E1 and E3 bind to the core as peripheral subunits. Advances in structural biology, particularly cryo-EM, X-ray crystallography, and nuclear magnetic resonance (NMR), have provided valuable insights into PDHc organization, assembly principles, and species-specific variation. Here, we review diverse PDHc architectures across phylogenetic groups. Understanding these structural and functional adaptations is essential for fully deciphering PDHc regulation and its role in metabolism.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144845059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of visible light-sensitive human neuropsin (OPN5) via single amino acid substitution 通过单氨基酸取代制备可见光敏人神经素（OPN5）。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-13 DOI: 10.1002/1873-3468.70130

Yusuke Sakai, Richard J. McDowell, Robert J. Lucas

引用次数: 0

Autophagy in the regulation of cancer dormancy 自噬在调节癌症休眠中的作用。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-13 DOI: 10.1002/1873-3468.70139

Damla Gunes, Alara Ustal, Yusuf Emre Ertem, Yunus Akkoc, Devrim Gozuacik

引用次数: 0

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells. 探索脂质多样性和极简主义来定义合成细胞的膜要求。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-11 DOI: 10.1002/1873-3468.70131

Sergiy Gan, Victoria Scarpelli, Marten Exterkate

{"title":"Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells.","authors":"Sergiy Gan, Victoria Scarpelli, Marten Exterkate","doi":"10.1002/1873-3468.70131","DOIUrl":"https://doi.org/10.1002/1873-3468.70131","url":null,"abstract":"The creation of minimal synthetic cells that mimic the essential functions of biological cells is a long-term goal in synthetic biology. Achieving this objective not only advances our understanding of the origin of life, but also unlocks the way for applications in industry, medicine, etc. A key characteristic of life is self-reproduction, which includes growth and division of the cell and its membrane. This boundary layer is formed by a lipid matrix in which proteins are anchored. The complexity of natural lipid membranes is a major challenge for the construction of a minimal system, as it directly influences membrane shape and protein function. Although simple synthetic compartmentalization systems can consist of a single lipid species, there is substantial uncertainty regarding the complexity of the lipidome required to sustain the essential functions of a self-reproducing cell. This Review highlights the contrast between bottom-up and top-down approaches toward synthetic cell construction, emphasizing the critical interplay between membrane proteins and their surrounding lipid environment. We explore the complexity and compatibility of membrane systems and discuss minimal lipidome requirements for synthetic cellular systems. Impact statement Synthetic cell research will help us to truly understand the basic principles of cellular life, in which lipid membranes are crucial. Ultimately, synthetic cells should lead to engineered specialized entities that can be applied in various fields, including medicine, bio-technology, and environmental science.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

D2-type dopamine receptors are required for LPS-induced acute hematopoiesis. 脂多糖诱导的急性造血需要d2型多巴胺受体。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-11 DOI: 10.1002/1873-3468.70143

Jia-Xin Yang, Yu-Yan Li, Zhao-Hua Deng, Tian-Jing Li, Ke Bai, Yan-Mei Yu, Jinjin Ma, Qi Chen, Yang Liu

引用次数: 0

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes. 多极端微生物在适应多种极端条件时产生的胞外多糖。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-08 DOI: 10.1002/1873-3468.70138

Tracey M Gloster, Ebru Toksoy Öner

{"title":"Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes.","authors":"Tracey M Gloster, Ebru Toksoy Öner","doi":"10.1002/1873-3468.70138","DOIUrl":"https://doi.org/10.1002/1873-3468.70138","url":null,"abstract":"Over the past few decades, research on polyextremophiles has revealed a diverse range of organisms adapted to multiple extreme conditions, such as combinations of high and low temperatures, acidity, pressure, salinity, and radiation. Under multiple extremes, a key survival mechanism is the production of exopolysaccharides (EPSs) via cell wall-associated or extracellular glycosyltransferases (GTs). EPSs not only protect cells against environmental extremes, desiccation, phage attacks, phagocytosis, and antibiotics; they also play important roles in inter- and intra-microbial interactions, quorum sensing, virulence, energy storage, and biofilm formation. Despite extensive studies on EPSs from extremophiles, knowledge on EPS production in polyextremophiles remains limited, particularly for psychrophiles, halophiles, and piezophiles. This review focuses on the adaptive strategies of polyextremophiles under multiple stress conditions, emphasizing the functional significance of EPS production. By providing an integrated perspective on polyextremophiles and their survival mechanisms, this work highlights the critical role of EPSs in their adaptation to extreme habitats and their potential biotechnological applications.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of mRNA metabolism in immune cells 免疫细胞mRNA代谢的调控。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-08 DOI: 10.1002/1873-3468.70132

Manendra Singh Negi, Blagoje Soskic, Ivano Legnini

引用次数: 0

C-mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells. c -甘露糖基化促进人睾丸生殖细胞瘤NEC8细胞ADAMTS1的激活和分泌。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-06 DOI: 10.1002/1873-3468.70133

Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu

引用次数: 0

Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy. 麦角硫因补充改善幼犬表型和小鼠脊髓性肌萎缩模型的存活率。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-06 DOI: 10.1002/1873-3468.70136

Francesca Cadile, Daniela Ratto, Giorgia Rastelli, Ottavia Eleonora Ferraro, Caterina Temporini, Sunil Kumar, Simona Boncompagni, Paola Rossi, Monica Canepari

{"title":"Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy.","authors":"Francesca Cadile, Daniela Ratto, Giorgia Rastelli, Ottavia Eleonora Ferraro, Caterina Temporini, Sunil Kumar, Simona Boncompagni, Paola Rossi, Monica Canepari","doi":"10.1002/1873-3468.70136","DOIUrl":"https://doi.org/10.1002/1873-3468.70136","url":null,"abstract":"Spinal muscular atrophy (SMA) is a genetic disorder characterized by the loss of spinal motor neurons. The conventional therapy does not always lead to a full restoration of the clinical symptoms, partially due to the need for early treatment. Accumulating evidence describes the crucial role of mitochondrial dysfunction and oxidative stress in skeletal muscle of SMA patients. We aimed to investigate the effects of prenatal supplementation with the antioxidant molecule ergothioneine (ERGO) on an SMNΔ7 mouse model of SMA containing a knockout of survival motor neuron protein (SMN1) and two transgenes, one with a single normal copy of human SMN2 and the second with a human SMN2 promoter and a human SMN2 cDNA lacking exon 7. ERGO had a significant positive effect on the survival and locomotor abilities of SMA pups. In isolated diaphragm muscle, ERGO was found to stimulate mitophagy. The results of the current study highlight the need for further research into ERGO as an adjuvant therapy for SMA. Impact statement Our finding that ergothioneine supplementation improves survival in a murine model of spinal muscular atrophy may aid research into a novel potential adjuvant to alleviate the symptoms of this serious neuromuscular disease in humans.","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building the lab together – from day one 一起建立实验室，从第一天开始。

IF 3 4区生物学

FEBS Letters Pub Date : 2025-08-06 DOI: 10.1002/1873-3468.70137

Hans-Georg Sprenger, Julia Hansen

引用次数: 0