Fish & shellfish immunology最新文献

{"title":"Exhaustive stress causes a rapid immunological response in the humoral and cellular haemolymph compartments of the pale octopus (Octopus pallidus)","authors":"Ryan D. Day ,&nbsp;Patricia Peinado ,&nbsp;Katherine B. Baker ,&nbsp;Jayson M. Semmens","doi":"10.1016/j.fsi.2024.109963","DOIUrl":"10.1016/j.fsi.2024.109963","url":null,"abstract":"<div><div>Cephalopods are economically and ecologically important species across the world, yet information linking physiological stress and associated immunological responses is limited in the current literature. Here, the effects of exhaustive exercise in a holobenthic octopus species, <em>Octopus pallidus</em>, were examined by evaluating immunological parameters. In whole haemolymph, the pH and refractive index were measured. To assess the cellular function of the haemolymph, the total count, cell vitality and phagocytosis capacity of the haemocytes were also measured. To assess enzymatic function, activities of the phenoloxidase system and lysozyme were quantified in the plasma and cellular components of the haemolymph. Overall, exhaustive exercise led to rapid changes in the haemolymph with a significant decrease in the pH and phagocytosis capacity though the number of cells and cell vitality were not affected. Exercise also triggered the increase of activated phenoloxidase (PO-like) activity and the decrease of the inactive zymogen prophenoloxidase (ProPO-like), total PO-like and lysozyme activity in plasma and an increase in total PO-like activity in the hemocyte compartment. These responses indicated that a realistic energetic demand had substantial, rapid impact on immune function. These results also provide an important baseline to understand the immune physiology of cephalopods that will further efforts to identify the mechanisms underlying the impacts of stressors.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109963"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Updates on infectious diseases of largemouth bass: A major review","authors":"Shun Yang ,&nbsp;Jing Zhao ,&nbsp;Nan An ,&nbsp;Dong-Chun Li ,&nbsp;Meng-Meng Huang ,&nbsp;Hui Fei","doi":"10.1016/j.fsi.2024.109976","DOIUrl":"10.1016/j.fsi.2024.109976","url":null,"abstract":"<div><div>The largemouth bass (<em>Micropterus salmoides</em>) is native to North America and has now become a crucial economic species in aquaculture. With the rapid development of high-density intensive farming models, the continuous emergence and spread of diseases pose significant challenges to the sustainable development of largemouth bass aquaculture, including <em>Micropterus salmoides</em> rhabdovirus (MSRV), largemouth bass virus (LMBV), <em>Nocardia</em> spp. and <em>Aeromonas</em> spp. Here, we provide a comprehensive overview of the latest research progress on common diseases of largemouth bass, including pathogen isolation and identification, pathological characteristics, morphological features, epidemiological characteristics, pathogen-host interactions, detection and diagnosis, vaccines, and other control technologies. This information will enhance a more comprehensive understanding of the occurrence of diseases in largemouth bass, and provide insights into future research directions, facilitating more effective disease prevention and control. The collaborative progress among rapid detection technology, the interaction mechanism between pathogen and host, and prevention and control techniques will be the curial to achieving green prevention and control of largemouth bass disease and healthy aquaculture in future.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109976"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor networks drive perforin activity in the anti-bacterial immune response of tilapia","authors":"Jie Cheng ,&nbsp;Ding Wang ,&nbsp;Ming Geng ,&nbsp;Yuying Zheng ,&nbsp;Yi Cao ,&nbsp;Shurong Liu ,&nbsp;Jiansong Zhang ,&nbsp;Jialong Yang ,&nbsp;Xiumei Wei","doi":"10.1016/j.fsi.2024.109975","DOIUrl":"10.1016/j.fsi.2024.109975","url":null,"abstract":"<div><div>Perforin, produced by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), is one of the effectors of cell-mediated cytotoxicity (CMC) in vertebrates, playing a paramount role in killing target cells. However, whether and how perforin is involved in adaptive immune responses in early vertebrates remains unclear. Using Nile tilapia (<em>Oreochromis niloticus</em>) as a model, we investigated the characteristics of perforin in early vertebrates. <em>Oreochromis niloticus</em> perforin (<em>OnPRF</em>) possesses 2 conserved functional domains, membrane attack complex/perforin (MACPF) and protein kinase C conserved region 2 (C2) domains, although they share low amino acid sequence similarity with other homologs. <em>OnPRF</em> was widely expressed in various immune tissues and could respond to lymphocyte activation and T-cell activation <em>in vitro</em> at both the transcriptional and protein levels, indicating that it may be involved in adaptive immune responses. Furthermore, after infection with <em>Edwardsiella piscicida</em> and <em>Aeromonas hydrophila</em>, the mRNA and protein levels of <em>OnPRF</em> were significantly up-regulated within the adaptive immune response period. Additionally, we revealed that many transcription factors were involved in the transcriptional regulation of <em>OnPRF</em>, including p65, c-Fos, c-Jun, STAT1 and STAT4, and there was a synergy among these transcription factors. Overall, these findings demonstrate the involvement of <em>OnPRF</em> in T-cell activation and adaptive immune response in tilapia, thus providing new evidence for comprehending the evolution of immune response in early vertebrates.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109975"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Passive immunoprophylaxis with Ccombodies against Vibrio parahaemolyticus in Pacific white shrimp (Penaeus vannamei)","authors":"Wanna Sirimanapong ,&nbsp;Prawporn Thaijongrak ,&nbsp;Chiranan Sudpraseart ,&nbsp;Dennis Berbulla Bela-ong ,&nbsp;Abigail Joy D. Rodelas-Angelia ,&nbsp;Mark Rickard N. Angelia ,&nbsp;Seungbeom Hong ,&nbsp;Jaesung Kim ,&nbsp;Kim D. Thompson ,&nbsp;Tae Sung Jung","doi":"10.1016/j.fsi.2024.109973","DOIUrl":"10.1016/j.fsi.2024.109973","url":null,"abstract":"<div><div>The <em>Vibrio parahaemolyticus</em> strain causing acute hepatopancreatic necrosis disease (AHPND) in shrimp secretes toxins A and B (PirA^<em>Vp</em>/PirB^<em>Vp</em>). These toxins have been implicated in pathogenesis and are targets for developing anti-AHPND therapeutics or prophylactics that include passive immunization. We have previously reported that Ccombodies (recombinant hagfish variable lymphocyte receptor B antibodies; VLRB) targeting PirB^<em>Vp</em> conferred protection against <em>V. parahaemolyticus</em> in shrimp when administered as a feed supplement. In this study, we screened a phage-displayed library of engineered VLRBs for PirA^<em>Vp</em>-targeting Ccombodies that were mass-produced in a bacterial expression system. We then introduced these Ccombodies into the diet of Pacific white shrimp (<em>Penaeus vannamei</em>) over a seven-day period. Subsequently, the shrimp were exposed to a challenge with <em>V. parahaemolyticus</em>. Mortality rates were then observed and recorded over the following seven days. Administering shrimp feed supplemented with Ccombodies at a high dose (100 mg per 100 g feed) reduced mortality in recipient animals (2.96–5.19 %) statistically similar to mock-challenged control (1.48 %), but significantly different from the Ccombody-deficient control (74.81 %). This suggests that the Ccombodies provided strong protection against the bacterium. Feeding shrimp with a median dose (10 mg/100 g feed) gave statistically comparable low mortality (5.93–6.67 %) as the high dose. Reducing the Ccombody dose to 1 mg/100 g feed showed variable effects. Ccombody A2 showed mortality (11.85 %) significantly lower than that of the Ccombody-deficient group (74.81 %), suggesting that it can effectively protect against the bacterial challenge at a low dose. Our results demonstrate the ability of the phage-displayed VLRB library to generate antigen-specific Ccombodies rapidly and simply, with the expression of high protein levels in bacteria. The protective effect provided by these Ccombodies aligns with our earlier results, strongly supporting the use of VLRB antibodies as a substitute for IgY in passive immunoprophylaxis against AHPND in shrimp.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109973"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles and citrullination signatures are novel biomarkers in sturgeon (Acipenser gueldenstaedtii) during chronic stress due to seasonal temperature challenge","authors":"Ana María Ferreira ,&nbsp;Valeria Silva-Álvarez ,&nbsp;Igor Kraev ,&nbsp;Pinar Uysal-Onganer ,&nbsp;Sigrun Lange","doi":"10.1016/j.fsi.2024.109974","DOIUrl":"10.1016/j.fsi.2024.109974","url":null,"abstract":"<div><div><em>Acipenser gueldenstaedtii</em> is one of the most cultured sturgeon species worldwide and of considerable economic value for caviar production. There are though considerable challenges around chronic stress responses due to increased summer temperatures, impacting sturgeons’ immune responses and their susceptibility to opportunistic infections. The identification of molecular and cellular pathways involved in stress responses may contribute to identifying novel biomarkers reflective of fish health status, crucial for successful sturgeon aquaculture. Protein citrullination is a calcium-catalysed post-translational modification caused by peptidylarginine deiminases (PADs), altering target protein function and affecting protein interactions in physiological and pathobiological processes. PADs can also modulate extracellular vesicle (EVs) profiles, which play critical roles in cellular communication, via transport of their cargoes (proteins, including post-translationally modified proteins, genetic material and micro-RNAs).</div><div>This study identified differences in EV signatures, and citrullinated proteins in sera from winter and summer farmed sturegeons. EVs were significantly elevated in sera of the summer chronically stressed group. The citrullinated proteins and associated gene ontology (GO) pathways in sera and serum-EVs of chronically heat stressed <em>A. gueldenstaedtii</em>, showed some changes, with specific citrullinated serum protein targets including alpa-2-macroglobulin, alpha globin, calcium-dependent secretion activator, ceruloplasmin, chemokine XC receptor, complement C3 isoforms, complement C9, plectin, selenoprotein and vitellogenin. In serum-EVs, citrullinated protein cargoes identified only in the chronically stressed summer group included alpha-1-antiproteinase, apolipoprotein B-100, microtubule actin crosslinking factor and histone H3. Biological gene ontology (GO) pathways related to citrullinated serum proteins in the chronically stressed group were associated with innate and adaptive immune responses, stress responses and metabolic processes. In serum-EVs of the heat-stressed group the citrullinome associated with various metabolic GO pathways.</div><div>In addition to modified citrullinated protein content, Serum-EVs from the stressed summer group showed significantly increased levels of the inflammatory associated miR-155 and the hypoxia-associated miR-210, but significantly reduced levels of the growth-associated miR-206.</div><div>Our findings highlight roles for protein citrullination and EV signatures in response to chronic heat stress in <em>A. gueldenstaedtii</em>, indicating a trade-off in immunity versus growth and may be of value for sturgeon aquaculture.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109974"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary succinic acid supplementation on growth performance, digestive ability, intestinal development and immunity of large yellow croaker (Larimichthys crocea) larvae","authors":"Chenxiang Zhang ,&nbsp;Yongtao Liu ,&nbsp;Zhijie Shi ,&nbsp;Chuanwei Yao ,&nbsp;Jianmin Zhang ,&nbsp;Yuntao Wang ,&nbsp;Jiahui Liu ,&nbsp;Kangsen Mai ,&nbsp;Qinghui Ai","doi":"10.1016/j.fsi.2024.109972","DOIUrl":"10.1016/j.fsi.2024.109972","url":null,"abstract":"<div><div>The application of artificial micro-diet is an effective way to improve and standardize the quality of aquatic animal larvae. However, the widespread adoption of micro-diet faces a bottleneck due to the limited utilization capacity of the larvae. A 30-day feeding experiment was carried out to investigate the effect of dietary succinic acid (SA) on the growth performance, digestive ability, intestinal development, and immunity of large yellow croaker larvae (initial body weight 11.33 ± 0.57 mg). Four isonitrogenous and isolipidic diets were formulated, incorporating 0.00 %, 0.01 %, 0.02 % and 0.03 % SA separately. The results showed that a diet with 0.02 % SA significantly increased both the final body weight and the specific growth rate of the larvae. Regarding digestive ability, 0.01 % SA supplementation significantly enhanced trypsin activity in both intestinal and pancreatic segments. In addition, 0.01 % SA supplementation notably improved amylase activity in the intestinal segment, while diets with 0.01%–0.02 % SA significantly improved lipase activity in the pancreatic segment. In terms of intestinal development, 0.01 % SA supplementation remarkably boosted the activities of alkaline-phosphatase and leucine-aminopeptidase on brush border membrane in intestine. Furthermore, 0.03 % SA supplementation significantly increased the expression of <em>occludin</em>. In terms of immunity, larvae fed diets with 0.01%–0.02 % SA exhibited significantly higher lysozyme activity compared to the control group. Supplementation with 0.01 % SA also significantly increased both iNOS activity and NO content. In summary, the findings of this study suggested that supplementing 0.02 % SA can improve the growth performance of large yellow croaker larvae by improving digestive enzymes activities, promoting intestinal development, and enhancing nonspecific immunity.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"155 ","pages":"Article 109972"},"PeriodicalIF":4.1,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the probiotic potential of Lactobacillus plantarum: Antioxidant capacity, non-specific immunity and intestinal microbiota improvement effects on Manila clam Ruditapes philippinarum","authors":"Longzhen Liu ,&nbsp;Haonan Zhuang ,&nbsp;Xiangli Tian ,&nbsp;Yujia Zhou ,&nbsp;Fangyi Wang ,&nbsp;Zirong Liu ,&nbsp;Jiamin Li ,&nbsp;Minghui Jiao ,&nbsp;Suyan Xue ,&nbsp;Jiaqi Li ,&nbsp;Weiwei Jiang ,&nbsp;Yuze Mao","doi":"10.1016/j.fsi.2024.109971","DOIUrl":"10.1016/j.fsi.2024.109971","url":null,"abstract":"<div><div>Lactic acid bacteria (LAB) have beneficial effects on aquatic animals, improving their immune system and intestinal microbiota. Nevertheless, the probiotic effects of LAB on the Manila clam <em>Ruditapes philippinarum</em> remain poorly understood. Herein, the effects of administering <em>Lactobacillus plantarum</em> at final doses of 1 × 10⁵ CFU/L (T5 group), 1 × 10⁷ CFU/L (T7 group), and 1 × 10⁹ CFU/L (T9 group) in the rearing water for eight weeks were evaluated for the antioxidant capacity, non-specific immunity, resistance to <em>Vibrio parahaemolyticus</em> infection, and intestinal microbiota of <em>R</em>. <em>philippinarum</em>. The rearing water without the addition of <em>L. plantarum</em> served as a control. The results showed that the T7 and T9 groups demonstrated a significant elevation in the disease resistance of clams against <em>V</em>. <em>parahaemolyticus</em>, in the activities of alkaline phosphatase and lysozyme in the hepatopancreas, and in the expression of antioxidant- and immune-related genes, including <em>SOD</em>, <em>GPx</em>, and <em>GST</em>. Meanwhile, the T7 group showed a significant enhancement in superoxide dismutase and catalase activities and <em>CAT</em> expression, while the T9 group experienced a remarkable elevation in reduced glutathione content. Only catalase activity was markedly elevated in the T5 group. The expression of <em>SOD</em>, <em>CAT</em>, <em>GPx</em>, and <em>GST</em> was significantly elevated in three treatment groups following the <em>V. parahaemolyticus</em> challenge. The T7 group exhibited a significant increase in intestinal microbiota richness. Significant increases were noted in Firmicutes abundance across all three treatment groups and in Actinobacteriota in the T5 and T7 groups. Additionally, the opportunistic pathogen <em>Escherichia-Shigella</em> abundance significantly decreased in three treatment groups. Furthermore, administration of 1 × 10⁷ CFU/L <em>L. plantarum</em> enhanced the stability of the intestinal microecosystem, whereas a dose of 1 × 10⁹ CFU/L might have a negative effect. The application of three doses of <em>L. plantarum</em> significantly enhanced intestinal microbiota functions related to the immune response and oxidative stress regulation, while a higher dose (1 × 10⁹ CFU/L) might inhibit several functions. In conclusion, the application of <em>L. plantarum</em> in the rearing water exerted beneficial effects on the antioxidant capacity, non-specific immunity, resistance to <em>V</em>. <em>parahaemolyticus</em>, and the intestinal microbiota stability and functions of <em>R</em>. <em>philippinarum</em>. The beneficial effects of <em>L. plantarum</em> on <em>R</em>. <em>philippinarum</em> were dose-dependent, and the final dose of 1 × 10⁷ CFU/L exhibited the optimal effects.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109971"},"PeriodicalIF":4.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escherichia coli heat-labile enterotoxin B subunit as an adjuvant of mucosal immune combined with GCRV-II VP6 triggers innate immunity and enhances adaptive immune responses following oral vaccination of grass carp (Ctenopharyngodon idella)","authors":"Jiyuan Yin ,&nbsp;Huiliang Wu ,&nbsp;Wei Li ,&nbsp;Yingying Wang ,&nbsp;Yingying Li ,&nbsp;Xubing Mo ,&nbsp;Siming Li ,&nbsp;Yan Ren ,&nbsp;Houjun Pan ,&nbsp;Peng Jiang ,&nbsp;Qing Wang","doi":"10.1016/j.fsi.2024.109969","DOIUrl":"10.1016/j.fsi.2024.109969","url":null,"abstract":"<div><div>The grass carp reovirus (GCRV) is the most major pathogen that has threatened the grass carp (<em>Ctenopharyngodon idella</em>) industry of China for years. Though the oral vaccine has many advantages, the current vaccines still do not provide complete protection. Therefor the exploration of new preventive strategies is urgently needed. In this study, heat-labile enterotoxin B subunit of <em>Escherichia coli</em> (LTB) was combined with VP6 from GCRV type II (GCRV-II) via <em>Lactococcus lactis</em> expression system to form a potent oral vaccine and determines if fusion of LTB to the protective vaccine antigen can enhance protection in the fish. The expression of recombinant protein was confirmed by Western-blotting and enzyme-linked immunosorbent assay. The rare minnow was set as the model for the evaluation of the experiment administrated orally. The immune response including the antibody titer and the immune-related gene expression, and the protective efficacy which included the virus loaded and the relative protection, were thoroughly investigated after the trial. The results indicated that LTB can significantly elicit a higher neutralizing antibody responses and enhanced T-cell priming, activities and proliferation in mononuclear cells from intestine, spleen and kidney tissues when compared to the VP6 vaccine alone. Moreover, the combined adjuvant can significantly up-regulate type I interferon signaling in different immune organs, especially the mucosa associated lymphoidtissue which could not be induced by VP6 along, result in the contribution of the improvement in adaptive immune responses of the fish. In addition, challenge study showed that LTB combined VP6 could greatly improve the relative percent survival of the fish during the virus infection. These results highlight that LTB has the potential value to be a mucosal adjuvant of the fish, approaching for improving the efficacy of vaccination against GCRV-II, which does elicit both non-specific and specific immune responses.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109969"},"PeriodicalIF":4.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"bcIRF5 activates bcTBK1 phosphorylation to enhance PANoptosis during GCRV infection","authors":"Can Yang ,&nbsp;Jinwei Gao ,&nbsp;Hao Wu ,&nbsp;Zhenzhen Xiong ,&nbsp;Jun Xiao ,&nbsp;Yanfang Wu ,&nbsp;Qing Yang ,&nbsp;Zhonggui Xie ,&nbsp;Rui Song ,&nbsp;Dongsheng Ou ,&nbsp;Hao Feng","doi":"10.1016/j.fsi.2024.109968","DOIUrl":"10.1016/j.fsi.2024.109968","url":null,"abstract":"<div><div>TBK1 is an important IFN antiviral signalling factor, and in previous work black carp TBK1 (bcTBK1) and black carp IRF5 (bcIRF5) together promoted cell death in GCRV-infected cells. In this research, bcTBK1 and bcIRF5 were investigated both in vivo and in vitro to delineate their individual and combined functions. This study demonstrated that both bcTBK1 and bcIRF5 expressions were modulated in response to GCRV infection across the intestine, gill, kidney and spleen. In bcgill cells, overexpression of bcTBK1 and bcIRF5 initially suppressed the expression of cell death-related genes, including <em>RIPK1</em>, <em>caspase1</em>, <em>caspase3</em> and <em>bax</em>, but this suppression was negated upon GCRV infection. In vivo, mRNA expression levels of <em>RIPK1</em> and related genes varied by tissue following bcTBK1 or bcIRF5 overexpression and GCRV infection. Notably, intracellular co-overexpression of bcTBK1 and bcIRF5 led to significant upregulation of caspase3, caspase1, bax, and IL1β, along with enhanced caspase3 activity post-GCRV infection. This co-expression correlated with higher survival rates in black carp during GCRV infection and increased caspase3 mRNA in the spleen and gills. Hematoxylin-eosin (HE) staining indicated disorganized spleen tissue and edematous, hyperplastic gill changes in co-transfected groups after infection. TUNEL staining of tissue sections showed that DNA breakage was significantly stronger in the co-transfected group than in the other groups during GCRV infection. Further phosphorylation experiments showed that bcIRF5 promoted phosphorylation modification of bcTBK1. Thus, these data suggest that bcIRF5 activates bcTBK1 by enhancing its phosphorylation and promotes PANoptosis in GCRV-infected cells.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109968"},"PeriodicalIF":4.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, functional analysis of chitin-binding proteins and the association of its single nucleotide polymorphisms with Vibrio parahaemolyticus resistance in Penaeus vannamei","authors":"Shuang-Shuang Luo ,&nbsp;Xiu-Li Chen ,&nbsp;Ai-Jin Wang ,&nbsp;Qing-Yun Liu ,&nbsp;Min Peng ,&nbsp;Chun-Ling Yang ,&nbsp;Di-Gang Zeng ,&nbsp;Yong-Zhen Zhao ,&nbsp;Huan-Ling Wang","doi":"10.1016/j.fsi.2024.109966","DOIUrl":"10.1016/j.fsi.2024.109966","url":null,"abstract":"<div><div>Chitin-binding proteins (CBPs) play pivotal roles in numerous biological processes in arthropods, including growth, molting, reproduction, and immune defense. However, their function in the antibacterial immune defense of crustaceans remains relatively underexplored. In this study, twenty CBPs were identified and characterized in <em>Penaeus vannamei</em>. Expression profiling highlighted that the majority of CBPs were highly expressed in the intestine and hepatopancreas and responded to challenge by <em>Vibrio parahaemolyticus.</em> To explore the role of these CBPs in innate immunity, six CBPs (<em>PvPrg4</em>, <em>PvKrtap16</em>, <em>PvPT-1a</em>, <em>PvPT-1b</em>, <em>PvExtensin</em> and <em>PvCP-AM1159</em>) were selected for RNAi experiments. Silencing of only <em>PvPrg4</em> and <em>PvKrtap16</em> significantly decreased the cumulative mortality of <em>V. parahaemolyticus</em>-infected shrimp. Further studies demonstrated that inhibition of <em>PvPrg4</em> and <em>PvKrtap16</em> resulted in a marked upregulation of genes associated with the NF-κB and JAK-STAT signaling pathways, as well as antimicrobial peptides (AMPs), in both the intestine and hepatopancreas. These results collectively suggested that <em>PvPrg4</em> and <em>PvKrtap16</em> potentially promote <em>V. parahaemolyticus</em> invasion by negatively regulating the JAK-STAT and NF-κB pathways, thereby inhibiting the expression of AMPs. In addition, SNP analysis identified three SNPs in the exons of <em>PvPrg4</em> that were significantly associated with tolerance to <em>V. parahaemolyticus</em>. Taken together, these findings are expected to assist in the molecular marker-assisted breeding of <em>P. vannamei</em> associated with anti-<em>V. parahaemolyticus</em> traits, as well as expand our understanding of CBP functions within the immune regulatory system of crustaceans.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109966"},"PeriodicalIF":4.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}