European journal of protistology最新文献

Eltonia chernetsovi gen. et sp. nov. (Heterolobosea, Eutetramitea) from an ecologically diverse clade comprising closely related soil and seawater species Eltonia chernetsovi gen. et sp. 11 . （Heterolobosea, eutetritia）来自一个生态多样化的分支，包括密切相关的土壤和海水物种。

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-04-01 Epub Date: 2026-01-26 DOI: 10.1016/j.ejop.2026.126183

Ekaterina Piven , Alexander Kudryavtsev

{"title":"Eltonia chernetsovi gen. et sp. nov. (Heterolobosea, Eutetramitea) from an ecologically diverse clade comprising closely related soil and seawater species","authors":"Ekaterina Piven , Alexander Kudryavtsev","doi":"10.1016/j.ejop.2026.126183","DOIUrl":"10.1016/j.ejop.2026.126183","url":null,"abstract":"<div><div>A novel amoeboid protist, <em>Eltonia chernetsovi</em> gen. nov., sp. nov., was isolated from saline mud collected in the Elton Lake area, Russia (ca. 200–500‰) and characterized morphologically and molecularly. Only amoeboid cells and cysts were found. In amoebae, eruptive cytoplasmic flow and hyaline bulging are evident during non-directional movement. Cysts are spherical with a smooth wall. The nucleus has a large eccentric nucleolus. Experimental analysis of salinity tolerance shows that the strain, initially observed and cultivated in seawater with a salinity of 40‰, may survive freshwater conditions in an encysted form, but does not reproduce in freshwater conditions below 5‰ or in hypersaline conditions at 70‰ or higher. Amoebae demonstrate a short period of reproduction at a temperature of 37 °C; however, they rapidly encyst afterwards. Phylogenetic analyses of the SSU rRNA gene and ITS region place the new strain in a clade with two undescribed species from agricultural soil habitats, suggesting a broad range of biotopes where representatives of this clade may occasionally occur. We establish a new genus for this clade, which branches within Eutetramitea (Heterolobosea) and is related to the marine <em>Oramoeba fumarolia</em>, cf. <em>Stachyamoeba</em> sp. ATCC 50324, and the soil species <em>Vrihiamoeba italica</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"103 ","pages":"Article 126183"},"PeriodicalIF":1.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azadinium fusiforme, a new species of Amphidomataceae from Korean and Japanese coastal waters that lacks azaspiracid production 缺氮吡嗪酸的朝鲜和日本沿海水域的一新种Azadinium fususiforme

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-04-01 Epub Date: 2026-01-26 DOI: 10.1016/j.ejop.2026.126182

Urban Tillmann , Koyo Kuwata , Kyoungwon Cho , Kazuya Takahashi , Jan Tebben , Bernd Krock , Mitsunori Iwataki , Sunju Kim

{"title":"Azadinium fusiforme, a new species of Amphidomataceae from Korean and Japanese coastal waters that lacks azaspiracid production","authors":"Urban Tillmann , Koyo Kuwata , Kyoungwon Cho , Kazuya Takahashi , Jan Tebben , Bernd Krock , Mitsunori Iwataki , Sunju Kim","doi":"10.1016/j.ejop.2026.126182","DOIUrl":"10.1016/j.ejop.2026.126182","url":null,"abstract":"<div><div>The dinophyte family Amphidomataceae, comprising the genera <em>Azadinium</em> and <em>Amphidoma</em>, includes several species known to produce azaspiracids (AZAs), a group of lipophilic marine biotoxins associated with shellfish poisoning. Despite their ecological relevance and the increasing number of newly described species in recent years, their diversity is likely still underestimated, and knowledge of their global distribution and biogeography remains limited. Based on detailed morphological and phylogenetic analyses of strains from Korea and Japan, we describe <em>Azadinium fusiforme</em> sp. nov., a taxon that corresponds to the phylogenetic position of a strain previously reported from Japan. Light and scanning electron microscopy revealed that <em>Az. fusiforme</em> possesses a unique combination of morphological traits that distinguish it from its congeners. Notably, the ventral pore is located on the left side of the first apical plate, the pyrenoid is consistently positioned in the posteriormost part of the cell, the nucleus is elongated and centrally located, and pronounced thickenings occur along the sutures between the lateral and dorsal apical plates. Molecular data place <em>Az. fusiforme</em> in a well-supported, distinct clade within <em>Azadinium</em>, clearly supporting its designation as a new species. Mass spectrometric analyses showed no evidence of AZA production in any of the <em>Az. fusiforme</em> strains. This study contributes to a more fundamental understanding of the species diversity, distribution, and potential toxicity of Amphidomataceae in the Asia–Pacific region.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"103 ","pages":"Article 126182"},"PeriodicalIF":1.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioaccumulation of gadolinium in Tetrahymena: Phagocytotic uptake leads to the formation of gadolinium phosphate–organic composite particles 钆在四膜虫体内的生物积累：吞噬摄取导致磷酸钆-有机复合颗粒的形成

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2025-12-05 DOI: 10.1016/j.ejop.2025.126176

Jana Kohl-Chandramohan , Michael Schweikert , Tobias Junginger , Ingo Hartenbach , Norbert Klaas , Marie-Louise Lemloh

{"title":"Bioaccumulation of gadolinium in Tetrahymena: Phagocytotic uptake leads to the formation of gadolinium phosphate–organic composite particles","authors":"Jana Kohl-Chandramohan , Michael Schweikert , Tobias Junginger , Ingo Hartenbach , Norbert Klaas , Marie-Louise Lemloh","doi":"10.1016/j.ejop.2025.126176","DOIUrl":"10.1016/j.ejop.2025.126176","url":null,"abstract":"<div><div>The mechanisms of bioaccumulation in eukaryotic cells, along with the understanding of pathways and metal binding, are fundamental to advances in both basic and applied research. The ciliate <em>Tetrahymena pyriformis</em> bioaccumulates the rare earth element gadolinium (Gd) into food vacuoles and subsequently excretes Gd-containing particles. We investigated the uptake mechanism of Gd in detail and characterised the properties of the resulting biogenic particles. Our results demonstrate that <em>T. pyriformis</em> accumulates Gd<sup>3+</sup> ions via phagocytosis, with components of the organic cell culture medium playing a central role in mediating the uptake of Gd<sup>3+</sup> ions. Bioaccumulation occurred in cells cultivated in Gd<sup>3+</sup>-containing environmental water samples and in standard cell culture medium containing proteose peptone, but was absent when Gd<sup>3+</sup> ions were applied in complexed form, such as gadolinium-based contrast agents. The bioaccumulated and excreted microparticles are amorphous, display magnetic properties, and can be isolated. They consist of about 70 wt% amorphous gadolinium phosphate, 25–27 wt% organic material, and 3 wt% combined Ca, K, Mg, and Na. The individual components can be fractionated based on their solubility. These insights into microbial bioaccumulation of metal elements enhance our understanding of environmental elemental cycles and could provide new strategies for element recovery.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126176"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic reassessment of Ambiphrya and Apiosoma (Ciliophora, Peritrichia), with morpho-molecular characterization of two epibiotic ciliates from rainbow trout in Korea 韩国虹鳟鱼两种表生纤毛虫的形态-分子特征及其对两种纤毛虫的系统重新评价

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2026-01-06 DOI: 10.1016/j.ejop.2026.126180

Borong Lu , Hunter N. Hines , Weibo Song , Mann Kyoon Shin

{"title":"Systematic reassessment of Ambiphrya and Apiosoma (Ciliophora, Peritrichia), with morpho-molecular characterization of two epibiotic ciliates from rainbow trout in Korea","authors":"Borong Lu , Hunter N. Hines , Weibo Song , Mann Kyoon Shin","doi":"10.1016/j.ejop.2026.126180","DOIUrl":"10.1016/j.ejop.2026.126180","url":null,"abstract":"<div><div>Many peritrich ciliates are parasites of, or epibionts on, aquatic animals. Among them, the genera <em>Ambiphrya</em> and <em>Apiosoma</em> are common fish ectocommensals in aquacultures. Here, we report infections by two poorly known forms, <em>Ambiphrya</em> cf. <em>ameiuri</em> and <em>Apiosoma pseudopiscicola</em>, on rainbow trout, a commonly farmed and economically important food fish in South Korea, and provide detailed descriptions and diagnostic illustrations of both species. Their SSU rDNA sequences were obtained, filling the molecular data gap for <em>Apiosoma</em>, a genus for which no sequences had previously been available. Our phylogenetic analysis supports the exclusion of <em>Ambiphrya</em> from the family Scyphidiidae, although its familial placement remains unresolved. The valid species of <em>Ambiphrya</em> are clarified, and populations identified as <em>Ambiphrya ameiuri</em> appear to represent a species complex. The family rank of Apiosomatidae, proposed by Jankowski (2007) but not widely adopted, is validated. Our findings confirm that <em>Apiosoma</em> belongs to Apiosomatidae rather than to the family Epistylididae in the widely used classifications of Corliss (1979) and Lynn (2008). <em>Parapiosoma</em>, previously of uncertain familial placement, should likewise be assigned to Apiosomatidae. Notably, the proposed synonymization of Scyphidiidae with Apiosomatidae, as well as the inclusion of other genera in Apiosomatidae by Jankowski (2007), is unsupported.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126180"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and analysis of enoyl-CoA hydratase/isomerase family protein of Eimeria tenella 柔嫩艾美耳球虫烯酰辅酶a水合酶/异构酶家族蛋白的分子特征及分析

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2025-12-05 DOI: 10.1016/j.ejop.2025.126177

Shisi Zhang , Shunhai Zhu , Wenhao Huang , Qiping Zhao , Lu Wang , Hui Dong , Jenwen Wang , Yu Yu , Hongyu Han

{"title":"Molecular characterization and analysis of enoyl-CoA hydratase/isomerase family protein of Eimeria tenella","authors":"Shisi Zhang , Shunhai Zhu , Wenhao Huang , Qiping Zhao , Lu Wang , Hui Dong , Jenwen Wang , Yu Yu , Hongyu Han","doi":"10.1016/j.ejop.2025.126177","DOIUrl":"10.1016/j.ejop.2025.126177","url":null,"abstract":"<div><div>Avian coccidiosis is an important intestinal disease caused by <em>Eimeria</em> spp., which leads to significant economic losses in the poultry industry. Although anticoccidial drugs can effectively control it in intensive farming, their extensive and irrational use has induced drug resistance in <em>Eimeria</em> species. Our previous RNA-seq study revealed differential expression of the enoyl-CoA hydratase/isomerase family protein (<em>Et</em>ECHI) between drug-sensitive (DS) and drug-resistant <em>Eimeria tenella</em> strains. In this study, qPCR analysis showed that <em>Et</em>ECHI mRNA levels were significantly upregulated in diclazuril-resistant (DZR) and maduramicin-resistant (MRR) strains compared to the DS strain. Furthermore, <em>Et</em>ECHI transcription increased progressively with rising concentrations of MRR and DZR. Additionally, <em>Et</em>ECHI transcript levels were significantly higher in field-isolated diclazuril-resistant strains than in the DS strain. qPCR and western blot analyses showed that <em>Et</em>ECHI expression levels were highest in <em>E. tenella</em> unsporulated oocysts. Indirect immunofluorescence assays (IFA) revealed that <em>Et</em>ECHI was primarily localized in the cytoplasm of the parasite, with additional surface distribution. An in vitro inhibition assay demonstrated that anti-r<em>Et</em>ECHI polyclonal antibodies significantly reduced <em>E. tenella</em> sporozoites' invasion of DF-1 cells. These findings suggest that <em>Et</em>ECHI may be associated with the drug resistance of <em>E. tenella</em> and may play a role in parasite growth and development within host cells.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126177"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scottish nearshore monothalamid Foraminifera (Rhizaria): Description of five new species and one new genus 苏格兰近岸单thalamid有孔虫（根状目）：五个新种和一个新属的描述

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2026-01-06 DOI: 10.1016/j.ejop.2026.126181

Zaineb Henderson , Maria Holzmann , Andrew J. Gooday

{"title":"Scottish nearshore monothalamid Foraminifera (Rhizaria): Description of five new species and one new genus","authors":"Zaineb Henderson , Maria Holzmann , Andrew J. Gooday","doi":"10.1016/j.ejop.2026.126181","DOIUrl":"10.1016/j.ejop.2026.126181","url":null,"abstract":"<div><div>Henderson (2023) gave informal descriptions of several soft-walled, monothalamid foraminifera from intertidal zones in the Lorne area of northwest Scotland based on morphology. In the present study, we use a combination of morphological and molecular data to formally establish one new genus and five new monothalamid species from the same area. <em>Lorneia sphaerica</em> gen. & sp. nov. (monothalamid Clade D) has a spherical, coarsely agglutinated test containing magnetic particles and minute aperture-like openings distributed around the test. <em>Lorneia ovalis</em> gen. & sp. nov. (Clade D) has similar characteristics, but the test is oval, and there is a terminal aperture situated at each end. <em>Psammophaga owensi</em> sp. nov. (Clade E) has an oval, finely agglutinated test with a simple terminal aperture and intracellular magnetic particles. In <em>Hilla brevis</em> sp. nov. (Clade Y), the test is broadly oval and finely agglutinated with a reflective sheen and a large terminal aperture with a pronounced collar. <em>Flaviatella zaninettiae</em> sp. nov. (Clade Y) has an elongate, finely agglutinated test with a reflective sheen, a tubular terminal apertural structure, and distinctive yellow cytoplasm. Two species, <em>Flexammina islandica</em> Voltski and Pawlowski, 2015 and <em>Ovammina opaca</em> Dahlgren, 1962, are reported for the first time in Scottish coastal waters. This study underlines the importance and diversity of monothalamid foraminifera in coastal settings.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126181"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Step-by-step exploration of the diversity of the genus Bothrostoma Stokes, 1887 (Ciliophora, Armophorea) in Korea using morphological and molecular analyses 利用形态学和分子分析逐步探索Bothrostoma Stokes， 1887（纤毛蝗科，犰狳科）在韩国的多样性

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2025-11-24 DOI: 10.1016/j.ejop.2025.126174

Quoc Dung Nguyen, Nanda Dwi Kristanti, Novia Cahyani, Mann Kyoon Shin

{"title":"Step-by-step exploration of the diversity of the genus Bothrostoma Stokes, 1887 (Ciliophora, Armophorea) in Korea using morphological and molecular analyses","authors":"Quoc Dung Nguyen, Nanda Dwi Kristanti, Novia Cahyani, Mann Kyoon Shin","doi":"10.1016/j.ejop.2025.126174","DOIUrl":"10.1016/j.ejop.2025.126174","url":null,"abstract":"<div><div>The genus <em>Bothrostoma</em> Stokes, 1887 appears more diverse than previously reported. Recent studies have uncovered several new species, yet the full extent of its diversity remains to be revealed. In this study, we describe two new species, <em>Bothrostoma longicilia</em> nov. sp. and <em>B. dextroproboscis</em> nov. sp., collected from freshwater ponds in South Korea. Their characterization was conducted using both morphological and molecular approaches. <em>Bothrostoma longicilia</em> nov. sp. is distinguished by a combination of prominent caudal cilia, a short acute snout, 30–40 somatic kineties (including 11–15 postoral kineties), and cortical granules arranged in 7–8 discontinuous oblique rows between adjacent somatic kineties. <em>Bothrostoma dextroproboscis</em> nov. sp. is characterized by a rectangular shape, with the anterior left end terminating in a short acute snout and the right anterior end extended into a flexible proboscis, an oblong macronucleus, and an adoral zone of membranelles extending over approximately two-thirds of the body length. Molecular phylogenetic analysis based on SSU rDNA sequences supports the distinctness of these two new species and confirms the monophyly of the genus <em>Bothrostoma</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126174"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural data on stomatocysts of the genus Paraphysomonas (Chrysophyceae, Paraphysomonadales), including description of one new morphotype and identification of a new producer of Stomatocyst 52 副葡萄单胞菌属（chrysophyae, Paraphysomonadales）口囊的超微结构资料，包括一个新形态的描述和一个新生产者的鉴定52

IF 1.6 2区生物学

European journal of protistology Pub Date : 2026-02-01 Epub Date: 2025-11-24 DOI: 10.1016/j.ejop.2025.126173

Alena D. Firsova, Anna Y. Bessudova, Yelena V. Likhoshway

{"title":"Ultrastructural data on stomatocysts of the genus Paraphysomonas (Chrysophyceae, Paraphysomonadales), including description of one new morphotype and identification of a new producer of Stomatocyst 52","authors":"Alena D. Firsova, Anna Y. Bessudova, Yelena V. Likhoshway","doi":"10.1016/j.ejop.2025.126173","DOIUrl":"10.1016/j.ejop.2025.126173","url":null,"abstract":"<div><div>Among the diverse morphotypes of siliceous stomatocysts, not all producer species have been identified. Using scanning electron microscopy, we determined the species affiliation of two <em>Paraphysomonas</em> stomatocyst morphotypes based on the presence of species-specific siliceous scales on their surfaces. A new morphotype, Stomatocyst 514 Firsova & Bessudova, is formally described, and its relationship to <em>Paraphysomonas circumvallata</em> Thomsen was established on the basis of attached plate-scales. The previously described morphotype Stomatocyst 52 Duff & Smol, 1991 emend. Duff et al., 1995 is related to the species <em>Paraphysomonas corynephora</em>. The ecology of these two species differs considerably: cyst formation in <em>P. circumvallata</em> was observed in the southern basin of Lake Baikal in August at a water temperature of 14.3 °C, whereas <em>P. corynephora</em> formed cysts in the Angara River in May at 4.8 °C. These findings expand the potential for analyzing the biogeography of these species and may be applied to paleoclimatic reconstructions. The morphological diversity and ultrastructural features of all currently known stomatocyst morphotypes within the genus <em>Paraphysomonas</em> are discussed, and an updated identification key is provided.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"102 ","pages":"Article 126173"},"PeriodicalIF":1.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal effects on Chlorella sorokiniana UCAM 001 growth and physiology in flat-plate photobioreactors in Morocco's arid climate 摩洛哥干旱气候下平板光生物反应器中小球藻ucam001生长和生理的季节性影响

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-10-01 DOI: 10.1016/j.ejop.2025.126171

Aafaf Krimech , Sofyan Sbahi , Ouafa Cherifi , Abdessamad Hejjaj , Richard Mugani , Naaila Ouazzani , Martin Kerner , Brahim Oudra , Laila Mandi

{"title":"Seasonal effects on Chlorella sorokiniana UCAM 001 growth and physiology in flat-plate photobioreactors in Morocco's arid climate","authors":"Aafaf Krimech , Sofyan Sbahi , Ouafa Cherifi , Abdessamad Hejjaj , Richard Mugani , Naaila Ouazzani , Martin Kerner , Brahim Oudra , Laila Mandi","doi":"10.1016/j.ejop.2025.126171","DOIUrl":"10.1016/j.ejop.2025.126171","url":null,"abstract":"<div><div>This study aims to investigate the seasonal variation in growth and physiology of <em>Chlorella sorokiniana</em> UCAM 001 strain using annual optimization monitoring with a groundwater medium in two outdoor flat-plate photobioreactors (PBRs), which were first scaled up at the Faculty of Sciences Semlalia (Marrakech, Morocco). The culture medium was adjusted to ensure non-limiting nutrient concentrations (10 mg·L<sup>−1</sup> and 100 mg·L<sup>−1</sup> of additional phosphorus and nitrate, respectively). Temperature, light, and algal growth were monitored daily. Proline, glycine betaine, and catalase levels were measured every four days to assess the degree of algal stress. Biomass productivity increased during spring, reaching 30 mg·L<sup>−1</sup>·day<sup>−1</sup> with a specific growth rate of 0.73 day<sup>−1</sup>. However, no growth was observed during the summer. Physiological analysis revealed increased proline and glycine betaine levels during autumn and winter due to temperatures as low as 13 °C. In contrast, catalase concentration peaked in spring. Pearson correlation analysis indicated that nutrient limitation, together with temperature and light intensity, induced stress in <em>C. sorokiniana</em>, stimulating catalase production. Algal growth efficiently removed nutrients from the medium, achieving removal rates of 97 % for total phosphorus and 87 % for total nitrogen. Optimized cooling systems will improve PBR efficiency and support algal growth under extreme summer conditions.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"101 ","pages":"Article 126171"},"PeriodicalIF":1.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis of the anaerobic ciliate Tropidoatractus levanderi (Ciliophora, Armophorea, Metopida), with notes on conjugation and molecular phylogeny 厌氧纤毛虫troidatractus leanderi（纤毛虫目，纤毛虫目，Metopida目）的形态发生及其偶联和分子系统发育的注释

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-10-01 Epub Date: 2025-09-15 DOI: 10.1016/j.ejop.2025.126166

Xiaochen Feng , Wenbao Zhuang , Ran Li , Xiaozhong Hu

引用次数: 0