European journal of protistology最新文献

A remarkable new monothalamid (Rhizaria, Foraminifera) from the shoreline of Livingston Island, Antarctica

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-03-29 DOI: 10.1016/j.ejop.2025.126148

Marcel Dominik Solbach , Ferry Siemensma , Maria Holzmann

引用次数: 0

Characterization of the macronuclear and micronuclear pheromone genes of Euplotes raikovi reveals the origin of the mating type genetic diversity

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-03-22 DOI: 10.1016/j.ejop.2025.126146

Jianjun Huang , Ruitao Gong , Khaled A.S. Al-Rasheid , Feng Gao , Adriana Vallesi , Yaohan Jiang

{"title":"Characterization of the macronuclear and micronuclear pheromone genes of Euplotes raikovi reveals the origin of the mating type genetic diversity","authors":"Jianjun Huang , Ruitao Gong , Khaled A.S. Al-Rasheid , Feng Gao , Adriana Vallesi , Yaohan Jiang","doi":"10.1016/j.ejop.2025.126146","DOIUrl":"10.1016/j.ejop.2025.126146","url":null,"abstract":"<div><div>Ciliates produce diffusible, cell-type-specific pheromones to regulate growth and mating. In <em>Euplotes</em>, these signaling molecules belong to species-specific families of disulfide-rich and structurally homologous proteins. Pheromones are co-dominantly expressed by genes in the somatic macronucleus (MAC), whereas their allelic diversity originates from the <em>mating type locus</em> in the germline micronucleus (MIC). During MAC development in sexual process, the MIC-derived diversity of specific alleles is rearranged via macronucleus-destined sequences (MDSs) assembly. While many MAC pheromones are well characterized, their MIC precursors and rearrangement process remain unknown. Here, we identified two MAC pheromone genes (<em>mac-er-13/14</em>) of <em>E. raikovi</em>, and two MIC regions (19 kb in total) containing 10 MDSs that assemble into <em>mac-er-13</em>. These MDSs are separated by internal eliminated sequences (234–3345 bp). The shortest MDSs (9–36 bp) encode the secreted region of pheromone, while longer MDSs (44–419 bp) encode other regions. Considering that the secreted regions show a higher sequence variation and the shorter MDSs have higher probability of alternative processing or imprecise assembly, we hypothesize that the high sequence variability of the macronuclear pheromone genes, which underlies the large number of mating types in <em>E. raikovi</em>, may result from alternative processing or imprecise assembly of these short MDSs.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"99 ","pages":"Article 126146"},"PeriodicalIF":1.9,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeny, morphology, and ultrastructure of sessilid peritrich ciliates in termite guts, Termitophrya africana and Doliophrys denislynni gen. nov., sp. nov.

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-03-07 DOI: 10.1016/j.ejop.2025.126140

Renate Radek , Janneke Hassler , Katja Platt , Hisar Ahmad , Thomas Fromm , Michaela Strüder-Kypke , David Sillam-Dussès , Jiří Synek , Jan Šobotník , Vincent Hervé , Andreas Brune

{"title":"Phylogeny, morphology, and ultrastructure of sessilid peritrich ciliates in termite guts, Termitophrya africana and Doliophrys denislynni gen. nov., sp. nov.","authors":"Renate Radek , Janneke Hassler , Katja Platt , Hisar Ahmad , Thomas Fromm , Michaela Strüder-Kypke , David Sillam-Dussès , Jiří Synek , Jan Šobotník , Vincent Hervé , Andreas Brune","doi":"10.1016/j.ejop.2025.126140","DOIUrl":"10.1016/j.ejop.2025.126140","url":null,"abstract":"<div><div>Sessilids (Oligohymenophorea, Peritrichia, Sessilida) reportedly colonize the gut of certain “higher termites” (family Termitidae), but only a single species, <em>Termitophrya africana</em> from <em>Jugositermes tuberculatus</em> (subfamily Apicotermitinae), has been described based on a drawing. Similar ciliates were observed in other Apicotermitinae but remained unspecified<em>.</em> Our SSU rRNA gene-based survey of peritrich ciliates in a broad range of Termitidae recovered distinct phylotypes from several Apicotermitinae (<em>Astalotermes</em>, <em>Jugositermes</em>, and <em>Phoxotermes</em>), albeit only from samples collected in Cameroon and not from all species of these genera. They form a monophyletic group in the family Epistylididae (Sessilida), with <em>Orborhabdostyla bromelicola</em> as the closest relative. Light, scanning, and transmission electron microscopy of the sessilid ciliates in <em>J. tuberculatus</em> revealed two morphotypes, which were assigned to their corresponding phylotypes by sequence analysis of capillary-picked single cells. One morphotype, which is highly contractile and broadens continuously towards the posterior end, matches the description of <em>Termitophrya africana</em>. The cells are attached by a posterior scopula with short cilia and are often covered with rod-shaped ectobionts. The other morphotype has a stockier, barrel-shaped body and a short, clearly demarcated anterior end and is always free of ectobionts. We designate it as <em>Doliophrys denislynni</em> gen. nov., sp. nov.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"99 ","pages":"Article 126140"},"PeriodicalIF":1.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Armaparvus browni sp. nov. (Amoebozoa, Cutosea) isolated from an inland brackish water biotope

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-03-04 DOI: 10.1016/j.ejop.2025.126139

Alexander Kudryavtsev , Mikhail Krendelev, Ekaterina Piven, Ekaterina Volkova

{"title":"Armaparvus browni sp. nov. (Amoebozoa, Cutosea) isolated from an inland brackish water biotope","authors":"Alexander Kudryavtsev , Mikhail Krendelev, Ekaterina Piven, Ekaterina Volkova","doi":"10.1016/j.ejop.2025.126139","DOIUrl":"10.1016/j.ejop.2025.126139","url":null,"abstract":"<div><div>A tiny amoebozoan with a locomotive size of about 10 μm was isolated from an inland brackish water biotope in the northwest of Russia. During locomotion, amoebae were flattened, with broad anterior hyaloplasm and short ventral subpseudopodia that appeared like small bumps. Electron microscopy shows that amoebae are enclosed in a flexible layer of flat oval scales embedded in a matrix and separated from the plasma membrane surface. These characters are in accordance with the phylogenetic placement of this strain, as reconstructed using the nuclear SSU rRNA, actin, and mitochondrial cytochrome <em>c</em> oxidase subunit 1 (Cox1) gene sequences. Morphology and molecular phylogenies warrant the description of the studied strain as a new species, <em>Armaparvus browni</em> sp. nov. This species shows a broad range of salinity tolerance, being able to reproduce in culture under salinity of 2.5 ‰, but not in 0.3 ‰ or artificial freshwater medium. This may be due to an adaptation to brackish water with oscillating salinity and hence <em>A. browni</em> probably cannot expand into truly freshwater habitats.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"98 ","pages":"Article 126139"},"PeriodicalIF":1.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and molecular phylogeny of a new hypotrichous ciliate, Sterkiella zhangi n. sp. (Ciliophora, Oxytrichidae)

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-03-02 DOI: 10.1016/j.ejop.2025.126141

Miaomiao Wang, Tao Hu, Shanshan Wang, Zhangwen Tong, Qianqian Wei, Xinpeng Fan

{"title":"Morphology and molecular phylogeny of a new hypotrichous ciliate, Sterkiella zhangi n. sp. (Ciliophora, Oxytrichidae)","authors":"Miaomiao Wang, Tao Hu, Shanshan Wang, Zhangwen Tong, Qianqian Wei, Xinpeng Fan","doi":"10.1016/j.ejop.2025.126141","DOIUrl":"10.1016/j.ejop.2025.126141","url":null,"abstract":"<div><div>The morphology, morphogenesis, and molecular phylogeny of a new hypotrichous ciliate, <em>Sterkiella zhangi</em> n. sp., which originated from terrestrial moss collected in east China, were studied using live observation, protargol staining, and the macronuclear SSU rRNA and mitochondrial cytochrome <em>c</em> oxidase subunit 1 gene sequences. The new species can be distinguished from its congeners by the following characteristics: three ordinary frontal cirri (an extra small frontal cirrus present in half of the individuals examined), an average of 15 left and 16 right marginal cirri, five transverse cirri, two macronuclear nodules, and cyst surface with irregular wrinkles but without spines. The 1:3:3:3:4:4 frontal-ventral-transverse anlagen fragmentation pattern of <em>S. zhangi</em> n. sp. indicates that the extra frontal cirrus is very likely a retained parental structure. Phylogenetic analyses revealed that the new species branches early in the subfamily Stylonychinae. The comparison of mitochondrial cytochrome <em>c</em> oxidase subunit 1 gene sequences of <em>Sterkiella</em> species shows that the intra-specific variation is much smaller than the inter-specific divergence. The well-known model organism <em>Oxytricha trifallax</em> has accumulated significant evolutionary differences and should be treated as a distinct species rather than a synonym of <em>S. histriomuscorum</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"98 ","pages":"Article 126141"},"PeriodicalIF":1.9,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology, morphogenesis, and molecular phylogeny of a new brackish water ciliate, Heterodeviata longa nov. spec. (Ciliophora, Hypotrichia)

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-02-22 DOI: 10.1016/j.ejop.2025.126138

Lijian Liao , Abdul Ghaffar , Xiaozhong Hu

{"title":"Morphology, morphogenesis, and molecular phylogeny of a new brackish water ciliate, Heterodeviata longa nov. spec. (Ciliophora, Hypotrichia)","authors":"Lijian Liao , Abdul Ghaffar , Xiaozhong Hu","doi":"10.1016/j.ejop.2025.126138","DOIUrl":"10.1016/j.ejop.2025.126138","url":null,"abstract":"<div><div>The morphology and morphogenesis of a new Stichotrichida species, <em>Heterodeviata longa</em> nov. spec., isolated from a brackish water habitat in Qingdao, China, were investigated using live observation and the protargol staining method. The new species is morphologically distinguished by possessing one buccal, three to five parabuccal and three frontal cirri, one long frontoventral row of ca. 12 cirri extending beyond mid-body, four left and two right marginal cirral rows, and two dorsal kineties: (1) left dorsal kinety bipolar, comprising 16–23 dikinetids, and with one to three caudal cirri at its rear end, while (2) right dorsal kinety comprising only two dikinetids. During morphogenesis, four frontal-ventral cirral anlagen form both in the proter and the opisthe. The parental adoral zone of membranelles remains intact and is completely inherited by the proter. The anlagen for marginal rows and the left dorsal kinety develop intrakinetally. The right dorsal kinety is generated dorsomarginally. The 18S rRNA gene sequence of the new species has a similarity of 99.6 % and 98.1 % with two other congeners. Phylogenetic analysis supported their close relationship and the monophyly of the genus <em>Heterodeviata</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"98 ","pages":"Article 126138"},"PeriodicalIF":1.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algal organic matter alters protistan community structure and assembly processes in coastal sediments

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-02-01 DOI: 10.1016/j.ejop.2025.126134

Arbaz Rehman , Jing Wang , Hao Yue , Xiuhong Zhang , Zelong Li

{"title":"Algal organic matter alters protistan community structure and assembly processes in coastal sediments","authors":"Arbaz Rehman , Jing Wang , Hao Yue , Xiuhong Zhang , Zelong Li","doi":"10.1016/j.ejop.2025.126134","DOIUrl":"10.1016/j.ejop.2025.126134","url":null,"abstract":"<div><div>Diatom blooms are a global ecological perturbation that releases algal organic matter (AOM), significantly affecting coastal ecosystems by altering microbial community dynamics. AOM, derived from algal cell lysis, may serve as a nutrient source influencing protistan communities. However, the effects of AOM on protistan ecology, including the community structure and assembly processes, remain largely unexplored in coastal sediments. In this study, we investigated the impact of AOM on the protistan community structure using macrogenomic analysis and high-throughput sequencing. The results revealed significant shifts in the protistan diversity (alpha and beta diversity) and community composition. Phototrophs and consumers were the primary functional groups affected, with their relative abundances significantly regulated by AOM, highlighting its functional-level impacts. Moreover, AOM influenced also the protistan community assembly, increasing the proportion of deterministic processes and altering the dynamic succession within the protistan co-occurrence network. Diatom blooms act as ecological filters, reducing diversity while promoting the dominance of specific functional groups. This study bridges the gap in understanding the AOM's role in shaping the ecological succession of protists in coastal sediments, offering valuable insights into the broader ecological impact of marine diatom blooms.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"97 ","pages":"Article 126134"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and phylogeny of Pararaabena dentata Wolska, 1968 and further insights into the molecular evolution of trichostome ciliates (Ciliophora, Litostomatea)

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-02-01 DOI: 10.1016/j.ejop.2025.126133

Gözde Gürelli , Fevziye Işıl Kesbiç

引用次数: 0

Leptomyxa mirabilis n. sp. – A new species of the genus Leptomyxa (Amoebozoa, Tubulinea) with a remarkable floating form and unusual cyst structure

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-02-01 DOI: 10.1016/j.ejop.2024.126132

Nikita S. Kulishkin, Alexey V. Smirnov

{"title":"Leptomyxa mirabilis n. sp. – A new species of the genus Leptomyxa (Amoebozoa, Tubulinea) with a remarkable floating form and unusual cyst structure","authors":"Nikita S. Kulishkin, Alexey V. Smirnov","doi":"10.1016/j.ejop.2024.126132","DOIUrl":"10.1016/j.ejop.2024.126132","url":null,"abstract":"<div><div>Variable body shape and significant variations in cell size make it difficult to distinguish species belonging to the genus <em>Leptomyxa</em>. At present, molecular data are becoming crucial for the identification of these amoebae. Among leptomyxids, the divergence of the 18S rRNA gene, mainly used to distinguish species, is low. Morphologically distinct species may have very similar 18S rRNA gene sequences and vice versa. However, some species exhibit remarkable morphological characters that can be combined with gene sequences to distinguish them reliably. In this paper, we describe a new terrestrial species of the genus <em>Leptomyxa</em> isolated from the Russian Far East, <em>Leptomyxa mirabilis</em> n. sp. Amoebae of this species are reticulate, flattened, and branched. This species has a unique floating form, previously undescribed among leptomyxids, and a remarkable cyst structure. The cysts of the studied species are often lobed or have a complex branched shape resembling the plasmodiocarps of some mycetozoa. Cysts can be single-walled or double-walled. The outer wall of a double-walled cyst encloses one or two multinucleate endocysts. In some cases, the wall of mature cysts is complex and multilayered. In addition, <em>Leptomyxa mirabilis</em> n. sp. differs from related leptomyxid species in the sequence of the 18S rRNA gene.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"97 ","pages":"Article 126132"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143283846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and morphometric investigations on testate amoebae from Mongolia with descriptions of a new genus and four new species 蒙古遗存变形虫的形态学和形态计量学研究及一新属和四新种描述。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-02-01 DOI: 10.1016/j.ejop.2024.126128

Dominika Łuców , Ferry Siemensma , Dashtseren Avirmed , Milena Obremska , Michał Słowiński , Izabela Zawiska

{"title":"Morphological and morphometric investigations on testate amoebae from Mongolia with descriptions of a new genus and four new species","authors":"Dominika Łuców , Ferry Siemensma , Dashtseren Avirmed , Milena Obremska , Michał Słowiński , Izabela Zawiska","doi":"10.1016/j.ejop.2024.126128","DOIUrl":"10.1016/j.ejop.2024.126128","url":null,"abstract":"<div><div>Testate amoebae are a polyphyletic and highly diverse group of unicellular protists, inhabiting various habitats and successfully used as indicators of property of environmental variables, providing information on hydrology, pH and pollution of ecosystems. However, despite numerous studies, there is still a lack of data regarding their species diversity, geographical distribution and population dynamics in various habitats from previously unexplored or poorly investigated regions such as Mongolia. In this study, we describe the morphology and morphometry of a new genus and four new species: <em>Armatura murmillo</em> gen. nov., sp. nov., <em>Trinema lenticularis</em> sp. nov., <em>T. parmularius</em> sp. nov. and <em>T. scutarius</em> sp. nov., derived from peat cores extracted from two fens in northern Mongolia. We provide one of the first case studies of testate amoebae from Mongolia, documenting 64 taxa. We also provide information on the morphology and variability of four lesser known species: <em>Centropyxis lapponica</em>, <em>Hyalosphenia insecta</em>, <em>Pyxidicula ornata</em> and <em>Schoenbornia smithi</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"97 ","pages":"Article 126128"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0