European journal of protistology最新文献

The mitochondrial citrate synthase from Tetrahymena thermophila does not form an intermediate filament 嗜热四膜虫的线粒体柠檬酸合成酶不形成中间丝。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126121

Stefano Lometto , Daniela Sparvoli , Gabriele Malengo , Thomas Heimerl , Georg K.A. Hochberg

{"title":"The mitochondrial citrate synthase from Tetrahymena thermophila does not form an intermediate filament","authors":"Stefano Lometto , Daniela Sparvoli , Gabriele Malengo , Thomas Heimerl , Georg K.A. Hochberg","doi":"10.1016/j.ejop.2024.126121","DOIUrl":"10.1016/j.ejop.2024.126121","url":null,"abstract":"<div><div>The mitochondrial citrate synthase (mCS) purified from the ciliate <em>Tetrahymena thermophila</em> has been reported to form intermediate-filament-like structures during conjugation and to self-assemble into fibers when recombinantly expressed. This would represent a rare example of a tractable and recent origin of a novel cytoskeletal element. In an attempt to investigate the evolutionary emergence of this behavior, we re-investigated the ability of <em>Tetrahymena</em>’s mCS to form filaments in vivo. Using strep-tagged mCS in <em>Tetrahymena</em> and monoclonal antibodies, we found no evidence of filamentous structures during conjugation or starvation. Extensive biochemical characterization of mCS revealed that the self-assembly of recombinant protein is triggered by a specific chemical moiety shared by MES and HEPES buffers used in previous studies. The absence of indicative phenotypes in fiber-deficient GFP-tagged mutants indicates that <em>Tetrahymena</em> mCS did not evolve a structural role in sexual reproduction or metabolic regulation.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126121"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testate amoebae from the wetlands of the Phobjikha Valley of Bhutan, the Eastern Himalayas 喜马拉雅山脉东部不丹 Phobjikha 山谷湿地的睾丸变形虫。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126125

Manfred Wanner , Ferry Siemensma , Indra Prasad Acharja , Jigme Tshering , Pema Khandu , Santa Lal Gajmer , Chöki Gyeltshen , Tshering Dorji , Kinley Tenzin , Satoshi Shimano

{"title":"Testate amoebae from the wetlands of the Phobjikha Valley of Bhutan, the Eastern Himalayas","authors":"Manfred Wanner , Ferry Siemensma , Indra Prasad Acharja , Jigme Tshering , Pema Khandu , Santa Lal Gajmer , Chöki Gyeltshen , Tshering Dorji , Kinley Tenzin , Satoshi Shimano","doi":"10.1016/j.ejop.2024.126125","DOIUrl":"10.1016/j.ejop.2024.126125","url":null,"abstract":"<div><div>The Kingdom of Bhutan in the Eastern Himalayas harbors a rich biodiversity. However, its detailed documentation still needs to be created, including not only macroorganisms but also protists. In the present study, as many as 105 taxa of testate amoebae were identified and directly counted in soil suspension samples collected from the wetlands of the Gangtey-Phobji Valley (generally Phobjikha), Wangdue Phodrang district. The most dominant were cosmopolitan species, e.g., <em>Euglypha rotunda</em>, <em>Trinema lineare</em>, <em>T. complanatum</em>, <em>T. enchelys</em>, <em>Phryganella acropodia</em>, and <em>Plagiopyxis declivis</em>. However, some taxa such as <em>Centropyxis deflandriana, C. stenodeflandriana</em>, <em>Hoogenraadia</em> cf<em>. humicola</em>, <em>Pareuglypha reticulata</em>, and <em>Sphenoderia chardezi</em> have a geographically restricted distribution and/or are considered rare. Some specimens could not be assigned to already described species (<em>Centropyxis dentata</em> sp. nov. and <em>Difflugia fusiforma</em> sp. nov.) or were highly similar to known species but differed in size or other taxonomically important characteristics (e.g., <em>Hoogenraadia</em> cf. <em>humicola</em> and <em>Planhoogenraadia</em> sp.). To our knowledge, this study is the first to present a comprehensive list of testate amoebae from Bhutan, including some new and rare species. The discovery of a remarkable diversity of testate amoebae in only a few soil samples suggests that this remote and largely unexplored region likely harbors many more intriguing species.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126125"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology, morphogenesis, and molecular phylogeny of Holostichides (Holostichides) parachardezi nov. spec. (Ciliophora, Hypotrichia, Urostylida), a new soil ciliate from China 来自中国的新土壤纤毛虫 Holostichides (Holostichides) parachardezi nov.(来自中国的一种新的土壤纤毛虫。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126124

Ziyan Liu, Kexin Wang, Chengliang Zhang, Yuwen Jiang, Ranran Song, Tengyue Zhang, Fengchao Li

{"title":"Morphology, morphogenesis, and molecular phylogeny of Holostichides (Holostichides) parachardezi nov. spec. (Ciliophora, Hypotrichia, Urostylida), a new soil ciliate from China","authors":"Ziyan Liu, Kexin Wang, Chengliang Zhang, Yuwen Jiang, Ranran Song, Tengyue Zhang, Fengchao Li","doi":"10.1016/j.ejop.2024.126124","DOIUrl":"10.1016/j.ejop.2024.126124","url":null,"abstract":"<div><div>A new urostylid ciliate, <em>Holostichides</em> (<em>Holostichides</em>) <em>parachardezi</em>, collected from a Chinese tropical city, was studied using morphological and molecular methods. Diagnostic features of the new species include: size 140–180 × 30–60 μm in vivo; 25–43 macronuclear nodules; colourless cortical granules; three to six frontoterminal cirri; a midventral complex composed of seven to eleven cirral pairs and one midventral row of five to eleven cirri; four bipolar dorsal kineties and six to nine caudal cirri in four groups; and a terrestrial habitat. Its ontogenesis follows the typical <em>Holostichides</em> mode in that (1) the proximal portion of the parental adoral zone of membranelles is partly renewed and the opisthe’s oral primordium originates very close to the midventral complex; (2) the last anlage <em>n</em> generates the frontoterminal cirral row, and the midventral complex is formed from anlage IV to <em>n</em> − 1; and (3) both marginal rows and dorsal kinety anlagen develop within the parental rows. The present SSU rDNA phylogenies corroborated the monophyly of the genus <em>Holostichides</em>.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126124"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O short-branch Microsporidia, where art thou? Identifying diversity hotspots for future sampling 短枝微孢子虫啊，你在哪里？为未来采样确定多样性热点

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126119

Megan Gross , Ľubomír Rajter , Frédéric Mahé , David Bass , Cédric Berney , Nicolas Henry , Colomban de Vargas , Micah Dunthorn

{"title":"O short-branch Microsporidia, where art thou? Identifying diversity hotspots for future sampling","authors":"Megan Gross , Ľubomír Rajter , Frédéric Mahé , David Bass , Cédric Berney , Nicolas Henry , Colomban de Vargas , Micah Dunthorn","doi":"10.1016/j.ejop.2024.126119","DOIUrl":"10.1016/j.ejop.2024.126119","url":null,"abstract":"<div><div>Short-branch Microsporidia were previously shown to form a basal grade within the expanded Microsporidia clade and to branch near the classical, long-branch Microsporidia. Although they share simpler versions of some morphological characteristics, they do not show accelerated evolutionary rates, making them ideal candidates to study the evolutionary trajectories that have led to long-branch microsporidian unique characteristics. However, most sequences assigned to the short-branch Microsporidia are undescribed, novel environmental lineages for which the identification requires knowledge of where they can be found. To direct future isolation, we used the EukBank database of the global UniEuk initiative that contains the majority of the publicly available environmental V4 SSU rRNA gene sequences of protists. The curated OTU table and corresponding metadata were used to evaluate the occurrence of short-branch Microsporidia across freshwater, hypersaline, marine benthic, marine pelagic, and terrestrial environments. Presence–absence analyses infer that short-branch Microsporidia are most abundant in freshwater and terrestrial environments, and alpha- and beta-diversity measures indicate that focusing our sampling effort on these two environments would cover a large part of their overall diversity. These results can be used to coordinate future isolation and sampling campaigns to better understand the enigmatic evolution of microsporidians’ unique characteristics.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126119"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disentangling the metabolic profile of rumen ciliates: A historical perspective and future directions 解析瘤胃纤毛虫的代谢特征：历史视角与未来方向

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126126

Valdirley de Souza Lima, Franciane Cedrola, Millke Jasmine Arminini Morales, Vera Nisaka Solferini

引用次数: 0

A new species of the Trichia botrytis complex from the cloud forest in Ecuador 厄瓜多尔云雾林中的一种新的 Trichia botrytis 复合菌种。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126123

Dmytro Leontyev , Edvin Johannesen , Gabriel Moreno , Iryna Yatsiuk , Martin Schnittler

引用次数: 0

Architecture of the flagellar apparatus and related structures in Kolkwitziella acuta: Towards a fine-structural characterization of pallium-feeding dinoflagellates (Protoperidiniaceae) Kolkwitziella acuta 的鞭毛器及相关结构：对食藻类甲藻（原甲藻纲）进行精细结构鉴定。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126120

Sandra C. Craveiro , Mariana S. Pandeirada , Øjvind Moestrup , António J. Calado

{"title":"Architecture of the flagellar apparatus and related structures in Kolkwitziella acuta: Towards a fine-structural characterization of pallium-feeding dinoflagellates (Protoperidiniaceae)","authors":"Sandra C. Craveiro , Mariana S. Pandeirada , Øjvind Moestrup , António J. Calado","doi":"10.1016/j.ejop.2024.126120","DOIUrl":"10.1016/j.ejop.2024.126120","url":null,"abstract":"<div><div>The fine-structural organization of the protoperidiniacean <em>Kolkwitziella acuta</em> was examined by SEM and TEM. Serial sections of five cells of <em>K. acuta</em> were used to study the architecture of basal bodies and associated roots, the pusular system, and the feeding apparatus. The basal bodies were inserted 1 µm apart at an angle of ca. 80°, and displayed the typical peridinioid features of associating with two roots each and having a layered connective linking the longitudinal microtubular root to the transverse striated root. The transverse flagellar canal was associated with a ‘sac pusule’, while the longitudinal flagellar canal was linked, via a pusule canal surrounded by a conspicuous layer of striated material, to a collecting chamber from which ca. 40 pusular tubes radiated. An extruded pallium was present, associated with a microtubular strand (the MSP) that extended anteriorly and progressively separated into six or seven groups of microtubules, with electron-opaque vesicles present along the MSP. A prominent striated collar surrounded the exit area of the pallium and was connected to the striated collars of both the transverse and longitudinal flagellar canals. The partial nuclear-encoded LSU rDNA sequence confirmed the identification as <em>K. acuta</em>. The currently known fine-structural features of pallium-feeding Protoperidiniaceae are summarized.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126120"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142389120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecology of free-living freshwater heterotrophic euglenoids: A summarizing review.

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 Epub Date: 2024-11-28 DOI: 10.1016/j.ejop.2024.126127

Jaroslav Kubín, Josef Juráň

引用次数: 0

Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb. nov. and two new Metacystis and Apolagynus species (Ciliophora, Prostomatea) Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb.

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126122

Limin Jiang , Qianhui Sun , Xiaoxuan Zhu , Hunter N. Hines , Xiaozhong Hu

{"title":"Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb. nov. and two new Metacystis and Apolagynus species (Ciliophora, Prostomatea)","authors":"Limin Jiang , Qianhui Sun , Xiaoxuan Zhu , Hunter N. Hines , Xiaozhong Hu","doi":"10.1016/j.ejop.2024.126122","DOIUrl":"10.1016/j.ejop.2024.126122","url":null,"abstract":"<div><div>Ciliates of the class Prostomatea show a broad spectrum of feeding strategies and often occur abundantly in various aquatic habitats, playing a vital role in the biogeochemical cycle. Due to their small cell size and simple structure, prostomateans were considered to be a group with a lower degree of morphological differentiation for a long time. However, recent research suggests that the diversity of this group of ciliates is higher than previously thought. In the present study, three prostomateans, collected from different localities in China and classified into three families, were examined using morphological and phylogenetic techniques. Our analyses revealed two new species, <em>Metacystis multitricha</em> sp. nov. and <em>Apolagynus spiralis</em> sp. nov., and suggested a transfer of <em>Prorodon abietum</em> Penard, 1922 to the genus <em>Pelagothrix</em>. Phylogenetic analyses corroborated the morphological classifications of the three species into the families Metacystidae, Lagynusidae, and Holophryidae, respectively, demonstrating that their diagnostic characteristics bear an evolutionary signal at the family level.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126122"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resource supply and intraspecific variation in inducible defense determine predator–prey interactions in an intraguild predation food web 资源供应和诱导性防御的种内变异决定了捕食者与被捕食者之间的相互作用。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126114

Fenja-Marie Möller , Sabine Flöder , Graziano Di Giuseppe , Stefanie Devi Moorthi

{"title":"Resource supply and intraspecific variation in inducible defense determine predator–prey interactions in an intraguild predation food web","authors":"Fenja-Marie Möller , Sabine Flöder , Graziano Di Giuseppe , Stefanie Devi Moorthi","doi":"10.1016/j.ejop.2024.126114","DOIUrl":"10.1016/j.ejop.2024.126114","url":null,"abstract":"<div><p>This study investigated the dynamics of reciprocal phenotypic plasticity entailing inducible defense and offense in freshwater ciliate communities in response to altered resource supply and the extent of intraspecific trait variation. Communities consisted of <em>Euplotes octocarinatus</em> (intraguild prey) capable of inducible defense to escape predation, <em>Stylonychia mytilus</em> (intraguild predator) capable of inducible offense to expand its prey spectrum, and <em>Cryptomonas</em> sp. (algal resource). The extent of inducible defense was tested in ten different <em>Euplotes</em> strains in response to freeze-killed <em>Stylonychia</em> concentrate, revealing significant differences in their width and length development. In a subsequent 30-day experiment, four strains were incubated in monoculture and mixture with <em>Stylonychia</em> under continuous and pulsed microalgae supply. The polyclonal <em>Euplotes</em> population outperformed the monoclonal populations, except one, which developed the most pronounced inducible defense and retained the highest biovolume. <em>Stylonychia</em> fluctuated in size, but dominated all communities irrespective of clonal composition. Pulsed resource supply promoted biovolume production of both species. However, periods of resource depletion resulted in more <em>Stylonychia</em> resting cysts, allowing <em>Euplotes</em> to resume growth. Our study provides new insights into interactions of induced defense and intraguild predation under variable environmental conditions, emphasizing the relevance of intraspecific trait variation for predator–prey interactions and community dynamics.</p></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126114"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000646/pdfft?md5=b430c9714d85d9e55e027513e4d873f8&pid=1-s2.0-S0932473924000646-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0