European journal of protistology最新文献_第2页

Ecology of free-living freshwater heterotrophic euglenoids: A summarizing review. 自由生活淡水异养拟绿藻生态学综述。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 Epub Date: 2024-11-28 DOI: 10.1016/j.ejop.2024.126127

Jaroslav Kubín, Josef Juráň

引用次数: 0

Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb. nov. and two new Metacystis and Apolagynus species (Ciliophora, Prostomatea) Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb.

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-10-01 DOI: 10.1016/j.ejop.2024.126122

Limin Jiang , Qianhui Sun , Xiaoxuan Zhu , Hunter N. Hines , Xiaozhong Hu

{"title":"Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb. nov. and two new Metacystis and Apolagynus species (Ciliophora, Prostomatea)","authors":"Limin Jiang , Qianhui Sun , Xiaoxuan Zhu , Hunter N. Hines , Xiaozhong Hu","doi":"10.1016/j.ejop.2024.126122","DOIUrl":"10.1016/j.ejop.2024.126122","url":null,"abstract":"<div><div>Ciliates of the class Prostomatea show a broad spectrum of feeding strategies and often occur abundantly in various aquatic habitats, playing a vital role in the biogeochemical cycle. Due to their small cell size and simple structure, prostomateans were considered to be a group with a lower degree of morphological differentiation for a long time. However, recent research suggests that the diversity of this group of ciliates is higher than previously thought. In the present study, three prostomateans, collected from different localities in China and classified into three families, were examined using morphological and phylogenetic techniques. Our analyses revealed two new species, Metacystis multitricha sp. nov. and Apolagynus spiralis sp. nov., and suggested a transfer of Prorodon abietum Penard, 1922 to the genus Pelagothrix. Phylogenetic analyses corroborated the morphological classifications of the three species into the families Metacystidae, Lagynusidae, and Holophryidae, respectively, demonstrating that their diagnostic characteristics bear an evolutionary signal at the family level.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"96 ","pages":"Article 126122"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resource supply and intraspecific variation in inducible defense determine predator–prey interactions in an intraguild predation food web 资源供应和诱导性防御的种内变异决定了捕食者与被捕食者之间的相互作用。

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126114

Fenja-Marie Möller , Sabine Flöder , Graziano Di Giuseppe , Stefanie Devi Moorthi

{"title":"Resource supply and intraspecific variation in inducible defense determine predator–prey interactions in an intraguild predation food web","authors":"Fenja-Marie Möller , Sabine Flöder , Graziano Di Giuseppe , Stefanie Devi Moorthi","doi":"10.1016/j.ejop.2024.126114","DOIUrl":"10.1016/j.ejop.2024.126114","url":null,"abstract":"<div>This study investigated the dynamics of reciprocal phenotypic plasticity entailing inducible defense and offense in freshwater ciliate communities in response to altered resource supply and the extent of intraspecific trait variation. Communities consisted of Euplotes octocarinatus (intraguild prey) capable of inducible defense to escape predation, Stylonychia mytilus (intraguild predator) capable of inducible offense to expand its prey spectrum, and Cryptomonas sp. (algal resource). The extent of inducible defense was tested in ten different Euplotes strains in response to freeze-killed Stylonychia concentrate, revealing significant differences in their width and length development. In a subsequent 30-day experiment, four strains were incubated in monoculture and mixture with Stylonychia under continuous and pulsed microalgae supply. The polyclonal Euplotes population outperformed the monoclonal populations, except one, which developed the most pronounced inducible defense and retained the highest biovolume. Stylonychia fluctuated in size, but dominated all communities irrespective of clonal composition. Pulsed resource supply promoted biovolume production of both species. However, periods of resource depletion resulted in more Stylonychia resting cysts, allowing Euplotes to resume growth. Our study provides new insights into interactions of induced defense and intraguild predation under variable environmental conditions, emphasizing the relevance of intraspecific trait variation for predator–prey interactions and community dynamics.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126114"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000646/pdfft?md5=b430c9714d85d9e55e027513e4d873f8&pid=1-s2.0-S0932473924000646-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of mixotrophic and heterotrophic chrysomonads of similar size regarding bacterivory and growth rate 大小相似的混养和异养金丝单胞菌在细菌吞噬和生长速度方面的比较

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126109

Tobias Pietsch, Hartmut Arndt

{"title":"Comparison of mixotrophic and heterotrophic chrysomonads of similar size regarding bacterivory and growth rate","authors":"Tobias Pietsch, Hartmut Arndt","doi":"10.1016/j.ejop.2024.126109","DOIUrl":"10.1016/j.ejop.2024.126109","url":null,"abstract":"<div>Small chrysomonads are important bacterivores in aquatic ecosystems with a high molecular diversity compared to low morphological differences observed by light microscopy. The high diversity of these morphologically almost indistinguishable species leads to the question to which extent their functional role in ecosystems differs and how their ecological traits can be defined. The present study investigates the prey size and population growth rate of different chrysomonad species. Eleven phylogenetically well-defined strains representing seven strains of heterotrophic and four strains of mixotrophic chrysomonads were compared. All investigated strains belonged to the same functional group of bacterivorous flagellates, feeding on the same bacteria size range, while population growth rates of chrysomonads depended on nutritional strategy and species-specific differences. We observed a high individual variability of growth rates within a population. Our results point to the necessity to consider not only differences in ecological traits among species but also among specimens within a population.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126109"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000592/pdfft?md5=7c67f02916851a93cc54e576f31261d8&pid=1-s2.0-S0932473924000592-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and molecular phylogeny of Euplotes baugilensis n. sp. (Ciliophora, Spirotrichea), with an illustrated key to Euplotes species with reduced cirri Euplotes baugilensis n. sp. (Ciliophora, Spirotrichea) 的形态学和分子系统发育，附带绘有图解的具有退化卷须的 Euplotes 种的检索表

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126110

Eun-Hye Do , Hye-In Kwon , Jeong Hyeon Yeo , Pablo Quintela-Alonso , Jae-Ho Jung

{"title":"Morphology and molecular phylogeny of Euplotes baugilensis n. sp. (Ciliophora, Spirotrichea), with an illustrated key to Euplotes species with reduced cirri","authors":"Eun-Hye Do , Hye-In Kwon , Jeong Hyeon Yeo , Pablo Quintela-Alonso , Jae-Ho Jung","doi":"10.1016/j.ejop.2024.126110","DOIUrl":"10.1016/j.ejop.2024.126110","url":null,"abstract":"<div>Euplotes baugilensis n. sp. was discovered in a temporary puddle that formed after rainfall on a mountain footpath near Gangneung-Wonju National University in Gangneung, South Korea. After isolation, a pure culture was established, and the new species was examined using live observation, silver-impregnation (protargol and ‘wet’ silver nitrate), scanning electron microscopy, and the analysis of the 18S rRNA gene sequence. Morphologically, E. baugilensis n. sp. is characterized by small body size (on average 49 × 31 µm in vivo), 9 ordinary fronto-ventral cirri (cirrotype-9) with one reduced cirrus V/2 (composed of four non-ciliated basal bodies), 5 transverse cirri, 7 or 8 dorsolateral kineties, 6 dorsal prominent ridges, and a dargyrome (silverline system) of double type. In this study, we have used a combination of morphological and molecular techniques to characterize E. baugilensis n. sp. and determine its phylogenetic position within the genus Euplotes. Molecular analysis using 18S rRNA gene sequences indicated that E. baugilensis n. sp. is most closely related to E. curdsi (with a sequence identity of 96.8 %).</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126110"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses 淡水绿藻 Closterium ehrenbergii 中过氧化氢酶基因的分子特征及其对非生物胁迫的推定功能

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126111

Hui Wang , Peiling Wu , Fengru Li , Jeongmin Shin , Jang-Seu Ki

{"title":"Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses","authors":"Hui Wang , Peiling Wu , Fengru Li , Jeongmin Shin , Jang-Seu Ki","doi":"10.1016/j.ejop.2024.126111","DOIUrl":"10.1016/j.ejop.2024.126111","url":null,"abstract":"<div>Catalases (CATs) are ubiquitous antioxidant enzymes that prevent cellular oxidative damage through the decomposition of H2O2. However, there is relatively little information on CAT in the worldwide-distributed freshwater green alga Closterium ehrenbergii. Here, we cloned the full-length catalase cDNA from C. ehrenbergii (CeCAT) and characterized its structural features and expressional responses against aquatic contaminants. The open reading frame of CeCAT was determined to be 1476 bp, encoding 491 amino acids with a theoretical molecular mass of 56.1 kDa. The CeCAT protein belongs to the NADPH-binding CAT family and might be located in the cytosol. BLAST and phylogenetic results showed that CeCAT had a high identity with CAT proteins from other microalgae and the water lily Nymphaea colorata (Streptophyta). The transcriptional levels of CeCAT were significantly upregulated by the metal copper and herbicide atrazine, but little affected by other tested metals (Ni and Cr) and endocrine-disrupting chemicals (polychlorinated biphenyl, PCB). The maximum expression was registered under 0.1 mg/L CuCl2 and 0.2 mg/L CuSO4 exposures. In addition, excess copper considerably increased production of reactive oxygen species in the cells. These results suggest that CeCAT may function to defend against oxidative stress in green algae and can respond specifically to different kinds of metals and herbicides.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126111"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ciliate diversity and growth rates in experimental recirculating aquaculture and aquaponics systems using microscopy 利用显微镜观察试验性循环水产养殖和鱼菜共生系统中纤毛虫的多样性和生长率

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126113

Stefanos Moschos , Konstantinos Ar. Kormas , Hera Karayanni

{"title":"Ciliate diversity and growth rates in experimental recirculating aquaculture and aquaponics systems using microscopy","authors":"Stefanos Moschos , Konstantinos Ar. Kormas , Hera Karayanni","doi":"10.1016/j.ejop.2024.126113","DOIUrl":"10.1016/j.ejop.2024.126113","url":null,"abstract":"<div>The function of recirculating aquaculture systems (RAS) relies on microbial communities, which convert toxic, fish-excreted ammonia into substances that can provide nutrients to plants as in the case of aquaponics systems. In the present study, heterotrophic protist communities of experimental sea water RAS and freshwater aquaponics systems were investigated using microscopy to characterize their diversity, natural abundance, and potential growth rates. Heterotrophic protist abundance was low (732 ± 21 to 5451 ± 118 ciliates L−1 and 58 ± 8 to 147 ± 18 nanoflagellates mL−1 in the aquaponics system and 78 ± 28 to 203 ± 48 ciliates L−1 in the RAS), which is in line with values typically reported for rivers. In the aquaponics system, ciliates grew faster in the fish rearing tanks (1.9 ± 0.01 to 1.21 ± 0.03 d−1 compared to 0.54 ± 0.03 to 0.79 ± 0.05 d−1 in the other compartments), while heterotrophic nanoflagellates grew slower in drain tanks downstream of the hydroponics compartment (0.5 ± 0.3 to 1.37 ± 0.05 d−1 and 4.09 ± 0.11 d−1 to 6.03 ± 0.34 d−1in the other compartments). Results indicated distinct niches and reduced microeukaryotic diversity at the end of the system’s operation cycle.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126113"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of trichocysts in Paramecium bursaria following artificial removal and infection with the symbiotic Chlorella variabilis 人工去除和感染共生变色小球藻后囊丝藻毛囊的定量分析

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126115

Hikaru Morita , Yuuki Kodama

{"title":"Quantitative analysis of trichocysts in Paramecium bursaria following artificial removal and infection with the symbiotic Chlorella variabilis","authors":"Hikaru Morita , Yuuki Kodama","doi":"10.1016/j.ejop.2024.126115","DOIUrl":"10.1016/j.ejop.2024.126115","url":null,"abstract":"<div>The ciliate Paramecium bursaria possesses cell organelles called trichocysts that have defensive functions. Paramecium bursaria is capable of symbiosis with Chlorella variabilis, and the symbiotic algae are situated in close proximity to the trichocysts. To clarify the relationship between trichocysts in P. bursaria and the presence or absence of the intracellular symbiotic C. variabilis, this study compared the regeneration capacity of trichocysts in alga-free and algae-bearing P. bursaria. In addition, trichocyst protein abundance was measured when alga-free P. bursaria specimens were artificially infected with Chlorella. After completely removing trichocysts from P. bursaria cells by treatment with lysozyme and observing them after 24 h, the percentage of regenerating trichocysts in the entire cell was significantly higher in alga-free cells than that in algae-bearing cells. We also developed a simple method for the isolation of high-purity trichocysts to quantify trichocyst protein amounts. There was a significant difference in the trichocyst protein abundance of P. bursaria before and one week after mixing with Chlorella (i.e., after the establishment of symbiosis with algae). This study shows the importance of trichocysts in alga-free P. bursaria as well as their competition with symbiotic C. variabilis for attachment sites during the algal infection process.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126115"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protist diversity and co-occurrence patterns obtained by metabarcoding of terricolous lichens, coastal cliffs and a microbial mat in the Atacama Desert, northern Chile 通过对智利北部阿塔卡马沙漠的陆生地衣、海岸悬崖和微生物垫进行代谢编码获得的原生生物多样性和共生模式

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126108

Eduardo Acosta, Frank Nitsche, Hartmut Arndt

{"title":"Protist diversity and co-occurrence patterns obtained by metabarcoding of terricolous lichens, coastal cliffs and a microbial mat in the Atacama Desert, northern Chile","authors":"Eduardo Acosta, Frank Nitsche, Hartmut Arndt","doi":"10.1016/j.ejop.2024.126108","DOIUrl":"10.1016/j.ejop.2024.126108","url":null,"abstract":"<div>Protists can endure challenging environments sustaining key ecosystem processes of the microbial food webs even under aridic or hypersaline conditions. We studied the diversity of protists at different latitudes of the Atacama Desert by massive sequencing of the hypervariable region V9 of the 18S rRNA gene from soils and microbial mats collected in the Andes. The main protist groups in soils detected in active stage through cDNA were cercozoans, ciliates, and kinetoplastids, while the diversity of protists was higher including diatoms and amoebae in the microbial mat detected solely through DNA. Co-occurrence networks from soils indicated similar assemblages dominated by amplicon sequence variants (ASVs) identified as Rhogostoma, Euplotes, and Neobodo. Microbial mat networks, on the other hand, were structured by ASVs classified as raphid-pennate diatoms and amoebae from the genera Hartmannella and Vannella, mostly negatively correlated to flagellates and microalgae. Additionally, our phylogenetic inferences of ASVs classified as Euplotes, Neobodo, and Rhogostoma were supported by sequence data of strains isolated during this study. Our results represent the first snapshot of the diversity patterns of culturable and unculturable protists and putative keystone taxa detected at remote habitats from the Atacama Desert.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126108"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000580/pdfft?md5=30f646a13589443cbaafd0a4351779e8&pid=1-s2.0-S0932473924000580-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Conchophthirus sinanodontae n. sp. (Ciliophora: Scuticociliatia), a new endocommensal ciliate of the freshwater mussel Sinanodonta woodiana from Korea Description of Conchophthirus sinanodontae n. sp. (Ciliophora: Scuticociliatia), a new endocommensal ciliate of the freshwater mussel Sinanodonta woodiana from Korea.

IF 1.9 2区生物学

European journal of protistology Pub Date : 2024-08-01 DOI: 10.1016/j.ejop.2024.126112

Kyu-Seok Chae , Jae-Ho Jung , Gi-Sik Min

{"title":"Description of Conchophthirus sinanodontae n. sp. (Ciliophora: Scuticociliatia), a new endocommensal ciliate of the freshwater mussel Sinanodonta woodiana from Korea","authors":"Kyu-Seok Chae , Jae-Ho Jung , Gi-Sik Min","doi":"10.1016/j.ejop.2024.126112","DOIUrl":"10.1016/j.ejop.2024.126112","url":null,"abstract":"<div>The morphology and molecular phylogeny of a new ciliate, Conchophthirus sinanodontae n. sp., which was discovered in the freshwater mussel Sinanodonta woodiana (Lea, 1834) from the Chilsancheon River, Buyeo-gun, South Korea, were investigated. The new species was characterized and could be distinguished from congeners by a combination of characters including the ovate body outline, four to six oral polykinetids deeply embedded in the upper wall of the buccal cavity, six to ten vestibular kineties, 34–49 ventral and 36–53 dorsal somatic kineties. The genetic differences among C. sinanodontae n. sp. and other congeners with available 18S rDNA sequences further support its distinctness. Moreover, the phylogenetic analyses based on the 18S rDNA sequences show that the new species clusters with other congeners, corroborating the monophyly of the genus Conchophthirus. The Conchophthirus clade nests within the cluster of Dexiotricha spp., Loxocephalus luridus, and Haptophrya spp.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"95 ","pages":"Article 126112"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0