European journal of protistology最新文献_第3页

Tales of the crown: An integrative approach to the testate amoeba Galeripora dentata (Ehrenberg, 1830) Siemensma, 2021 (Amoebozoa, Arcellinida, Arcellidae) 王冠的故事：对齿状变形虫Galeripora齿状变形虫的综合研究（Ehrenberg, 1830） Siemensma, 2021（变形虫目，阿米巴目，阿米巴科）

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-06-08 DOI: 10.1016/j.ejop.2025.126157

Nikola Bankov

引用次数: 0

Trait-based predictors of feeding ecology patterns in shelled microorganisms 有壳微生物取食生态模式的性状预测因子

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-23 DOI: 10.1016/j.ejop.2025.126160

Rafael L. Macêdo , Odete Rocha

{"title":"Trait-based predictors of feeding ecology patterns in shelled microorganisms","authors":"Rafael L. Macêdo , Odete Rocha","doi":"10.1016/j.ejop.2025.126160","DOIUrl":"10.1016/j.ejop.2025.126160","url":null,"abstract":"<div><div>Functional traits provide key insights into ecological strategies and evolutionary diversification. In this study, we analyzed a comprehensive trait dataset to investigate morphological predictors of feeding ecology in testate amoebae from the Northern Holarctic realm, focusing on variability across 372 species. We also examined whether trait diversity mirrors taxonomic richness at the family level. Morphological traits included shell length, shell width, aperture dimensions, shape, and covering. The Principal Component Analysis (PCA) indicated that Axis 1 predominantly represented variation in shell and aperture size, while Axis 2 was associated with differences in overall shape proportions. Bacterivorous species exhibited the greatest morphological and taxonomic diversity, spanning 21 families and 48 genera, with mixotrophs and predators occupying nested subsets of their broader morphospace. The regression analyses demonstrated significant associations between species richness and variation in protective features, including aperture rim morphology and the presence of spines. Decision tree models identified the aperture width-to-length ratio as a key predictor of feeding strategy, although classification accuracy was lower for mixotrophs and predators. Future research should integrate shell morphology with phylogenetic data to enhance ecological strategy predictions in testate amoebae and explore hypotheses regarding functional diversification across a broader geographical scale and within different environments.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126160"},"PeriodicalIF":1.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Typical and atypical cell division versus potential meiosis and sex in euglenids 真核细胞的典型和非典型细胞分裂与潜在减数分裂和性别

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-30 DOI: 10.1016/j.ejop.2025.126164

Andrej Jedlička , Olivier Barth , Diana Lihanová Bugajdová , Terézia Beck , Vanessa Gelanová , Juraj Krajčovič , Dominika Vešelényiová , Matej Vesteg

{"title":"Typical and atypical cell division versus potential meiosis and sex in euglenids","authors":"Andrej Jedlička , Olivier Barth , Diana Lihanová Bugajdová , Terézia Beck , Vanessa Gelanová , Juraj Krajčovič , Dominika Vešelényiová , Matej Vesteg","doi":"10.1016/j.ejop.2025.126164","DOIUrl":"10.1016/j.ejop.2025.126164","url":null,"abstract":"<div><div>Euglenids typically reproduce asexually by closed intranuclear orthomitosis and longitudinal cell division. The nucleus divides without the dissolution of the nuclear envelope, and the spindle is radially symmetric. Typical longitudinal cell division begins in the reservoir at the anterior end of the cell and the shape of the dividing cell gradually changes from a “Y” to a “V”. This paper reviews typical mitosis and cytokinesis in euglenids, alongside atypical cell division, in which a single cell produces more than two daughter cells, forming distinctive star-like structures. Atypical cell division is particularly observed under specific stress conditions, such as exposure to chemical substances (e.g., phenol and xylene), and in senescent cultures. Although euglenids are generally considered to be asexual, the presence of key meiotic genes in <em>Euglena gracilis</em> suggests the potential for sexual reproduction. Bioinformatic analyses and experimental approaches, including flow cytometry and fluorescence in situ hybridization (FISH), are discussed as potential methods for detecting meiosis, sex, and cell fusion in euglenids. This review contributes to a broader understanding of both typical and atypical cell division in euglenids and underscores the need for further research into these processes, including the possible occurrence of meiosis and sexual reproduction.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126164"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel taxa of the centrohelid heliozoans (Pterocystida and Panacanthocystida) from agricultural soils 农业土壤中圆心虫的新分类群（翼虫和泛棘虫）

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-08-21 DOI: 10.1016/j.ejop.2025.126165

Dmitry G. Zagumyonnyi , German A. Sozonov , Elena A. Gerasimova , Artem O. Belyaev , Olga N. Zagumyonnaya , Elizaveta E. Makarenko , Ruslan Kasseinov , Denis V. Tikhonenkov

{"title":"Novel taxa of the centrohelid heliozoans (Pterocystida and Panacanthocystida) from agricultural soils","authors":"Dmitry G. Zagumyonnyi , German A. Sozonov , Elena A. Gerasimova , Artem O. Belyaev , Olga N. Zagumyonnaya , Elizaveta E. Makarenko , Ruslan Kasseinov , Denis V. Tikhonenkov","doi":"10.1016/j.ejop.2025.126165","DOIUrl":"10.1016/j.ejop.2025.126165","url":null,"abstract":"<div><div>Soil protists play key roles in terrestrial ecosystems, influencing microbial dynamics, nutrient cycling, and plant health. In this study, we describe a new genus and species of centrohelid heliozoans, <em>Tellocystis perplexa</em> gen. et sp. nov. and <em>Raphidocystis akmolaensis</em> sp. nov., isolated from agricultural soil habitats, and characterize two other known cyst-forming centrohelid species from soil. <em>T. perplexa</em> gen. et sp. nov., studied using light and electron microscopy, possesses an unusual spine-scale morphology, featuring a small drop-shaped basal plate and a curved shaft. Phylogenetic analysis of the 18S rRNA gene unexpectedly placed this species within the clade previously referred to as ‘<em>Pinaciophora</em>’, which is nested within Pterista, alongside unpublished clones of ‘<em>Choanocystis perpusilla</em>’ from Franz Josef Land and Southern Transbaikalia. Based on the obtained data, we transferred <em>C. perpusilla</em> to the genus <em>Tellocystis</em>. <em>R. akmolaensis</em> sp. nov. is morphologically similar to <em>R. symmetrica</em>, but differs from it by having smaller cell and plate-scale sizes, and by formation of cyst scales that are distinct from those of its trophic phase. Phylogenetic analysis indicated that this species occupies a deep evolutionary position within Raphidocystidae, basal to <em>R. symmetrica</em>, <em>R. tubifera</em>, <em>R. ambigua</em>, and ‘<em>R. brunii</em>’. This study advances current knowledge of the diversity of soil heterotrophic protists, which play an essential role in regulating microbial populations, and clarifies the intricate taxonomy of some centrohelid heliozoans.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126165"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and multi-gene phylogeny of Rubrioxytricha hasllaensis n. sp. (Ciliophora, Spirotrichea) from South Korea. 韩国毛螺旋体Rubrioxytricha hasllaensis n. sp.（纤毛虫目，螺旋体目）的形态和多基因系统发育。

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-28 DOI: 10.1016/j.ejop.2025.126163

Ji Hye Moon, Atef Omar, Jae-Ho Jung

{"title":"Morphology and multi-gene phylogeny of Rubrioxytricha hasllaensis n. sp. (Ciliophora, Spirotrichea) from South Korea.","authors":"Ji Hye Moon, Atef Omar, Jae-Ho Jung","doi":"10.1016/j.ejop.2025.126163","DOIUrl":"10.1016/j.ejop.2025.126163","url":null,"abstract":"<p><p>The morphology and multi-gene phylogeny of Rubrioxytricha hasllaensis n. sp., a non-oxytrichid dorsomarginalian ciliate discovered in a brackish water habitat in South Korea, were investigated. The new species is characterized by a body size of 125-145 × 30-40 μm in vivo, colorless cytoplasm, yellow cortical granules, 30-36 adoral membranelles, 25-35 left and 25-38 right marginal cirri, a single caudal cirrus, and undulating membranes distinctly curved leftward. In phylogenetic analyses based on 18S rRNA gene sequences, the new species clusters with other Rubrioxytricha species, forming a monophyletic clade that groups with members of the genera Polystichothrix, Pseudogastrostyla, Pseudocyrtohymena, and Pseudocyrtohymenides. In phylogenetic analyses using concatenated 18S-ITS1-5.8S-ITS2 and partial 28S rRNA gene sequences, newly obtained sequences from Rubrioxytricha, Pseudocyrtohymena, and Pseudocyrtohymenides form a sister clade to the assemblage of soft-bodied hypotrichs possessing dorsal kinety fragmentation. In addition to identifying morphological apomorphies shared by Rubrioxytricha and its phylogenetically related genera, including the absence of dorsal kinety fragmentation, a reduced number of caudal cirri, and the presence of a bipolar dorsomarginal kinety, new generic characters are introduced for dorsomarginalian hypotrichs and used to improve the diagnosis of the genus Rubrioxytricha.</p>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"126163"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCNA's dual legacy in ciliates: Conserved replication scaffold and lineage-specific genome architect PCNA在纤毛虫中的双重遗产：保守的复制支架和谱系特异性基因组架构

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-24 DOI: 10.1016/j.ejop.2025.126162

Hongzhen Jiang , Haicheng Li , Chundi Wang , Yuanyuan Wang , Yongqiang Liu

{"title":"PCNA's dual legacy in ciliates: Conserved replication scaffold and lineage-specific genome architect","authors":"Hongzhen Jiang , Haicheng Li , Chundi Wang , Yuanyuan Wang , Yongqiang Liu","doi":"10.1016/j.ejop.2025.126162","DOIUrl":"10.1016/j.ejop.2025.126162","url":null,"abstract":"<div><div>Proliferating cell nuclear antigen (PCNA) is a central scaffold in eukaryotic DNA replication, repair, and epigenetic regulation. While its roles are well-characterized in canonical model organisms, ciliates, unicellular eukaryotes with nuclear dimorphism and programmed genome remodeling, offer unparalleled insights into PCNA's functional adaptability. Their unique biology, including replication-coupled DNA elimination, macronuclear amplification, and replication band dynamics, positions PCNA at the intersection of genome stability and plasticity. This review systematically compares ciliate and human PCNA through phylogenetic, structural, and functional analyses. We reveal that ciliate PCNAs, despite ∼50 % sequence divergence from mammals, preserve conserved trimerization interfaces and partner-binding motifs, as validated by AlphaFold3-predicted models of the classical model organism <em>Tetrahymena thermophila</em> and the emerging systems <em>Euplotes eurystomus</em> and <em>Stylonychia lemnae</em>. Functional summaries highlight lineage-specific innovations, including epigenetic regulation, paralog specialization for nuclear differentiation, and replication band assembly. These findings have broader implications for elucidating replication-coupled chromatin dynamics, the adaptive evolution of multiprotein complexes, and the use of non-model organisms such as <em>E. eurystomus</em> and <em>S. lemnae</em> to uncover conserved principles of genome biology.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126162"},"PeriodicalIF":1.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular phylogeny and taxonomy of three anaerobic ciliates including Bothrostoma aporobustum nov. spec. (Ciliophora, Metopida) 包括Bothrostoma aporobustum 11 . spec在内的3种厌氧纤毛虫的分子系统发育与分类

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-05-28 DOI: 10.1016/j.ejop.2025.126155

Wenbao Zhuang , Xiaochen Feng , Ran Li , Xiaozhong Hu

{"title":"Molecular phylogeny and taxonomy of three anaerobic ciliates including Bothrostoma aporobustum nov. spec. (Ciliophora, Metopida)","authors":"Wenbao Zhuang , Xiaochen Feng , Ran Li , Xiaozhong Hu","doi":"10.1016/j.ejop.2025.126155","DOIUrl":"10.1016/j.ejop.2025.126155","url":null,"abstract":"<div><div>The order Metopida is a species-rich taxon within the obligate anaerobic ciliate class Armophorea. Metopids have garnered increasing interest due to their potential to shed light on mitochondrial evolution and symbiotic relationship between eukaryotes and prokaryotes. However, the majority of metopid species remain poorly or incompletely studied, largely due to limitations in earlier research methodologies. In this study, three species, <em>Bothrostoma aporobustum</em> nov. spec., <em>Brachonella mitriformis</em> and <em>Planometopus contractus</em>, were examined using a morpho-molecular approach. The new species is distinguished by a short proboscis-shaped snout, an average of 33 somatic kineties, and 21 adoral membranelles. <em>Brachonella mitriformis</em> is characterized by a broad obpyriform body with a narrow and flattened posterior end, an average of 53 somatic kineties and 77 adoral membranelles, and unevenly distributed dikinetids on the preoral dome. Phylogenetic analyses confirmed the validity and monophyly of the genera <em>Bothrostoma</em> and <em>Brachonella</em>, and robustly resolved the phylogenetic position of <em>Brachonella mitriformis</em>. Representatives of geographically distant populations of <em>Planometopus contractus</em> are morphologically highly similar and cluster together with strong support in SSU rRNA gene phylogenies.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126155"},"PeriodicalIF":1.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First records of nivicolous myxomycetes (Amoebozoa) from Kazakhstan 哈萨克斯坦尼维菌黏菌（阿米巴虫）的首次记录

IF 1.6 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-24 DOI: 10.1016/j.ejop.2025.126161

Zhansaya Azirakhmet , Oleg Shchepin , Maho Inoue , Assiya Kussainova , Rakhmetkazhy Bersimbaev , Martin Schnittler

引用次数: 0

Revision of the Acanthocystis turfacea species complex, description of Acanthocystis pohjolensis n. sp. and notes on the phylogeny of Acanthocystis 革面棘囊藻（Acanthocystis turfacea）物种复合体的修订，pohjolensis n. sp.的描述和棘囊藻系统发育的注释

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-08-01 Epub Date: 2025-07-07 DOI: 10.1016/j.ejop.2025.126159

Savelii A. Poluzerov , Sofiya V. Dmitrovskaya

{"title":"Revision of the Acanthocystis turfacea species complex, description of Acanthocystis pohjolensis n. sp. and notes on the phylogeny of Acanthocystis","authors":"Savelii A. Poluzerov , Sofiya V. Dmitrovskaya","doi":"10.1016/j.ejop.2025.126159","DOIUrl":"10.1016/j.ejop.2025.126159","url":null,"abstract":"<div><div><em>Acanthocystis turfacea</em> Carter, 1863 is the type species of the centrohelid genus <em>Acanthocystis</em> Carter, 1863, which is characterized by bifurcated spine scales. In this study, the organism reported under the name <em>A. turfacea</em> by Zlatogursky and Klimov (2016) is reidentified as <em>A. hymenodes</em> Nicholls, 2023, based on morphological and morphometric evidence. Additionally, a centrohelid with a small cell diameter (7.9–11.9 μm) and distinctive scales, previously attributed to <em>A. turfacea</em>, was isolated from a marine habitat and is described here as a new species, <em>Acanthocystis pohjolensis</em> n. sp. Molecular phylogenetic analysis based on 18S rRNA gene sequences places <em>A. pohjolensis</em> and <em>A. hymenodes</em> in a basal position within the genus <em>Acanthocystis</em>, suggesting that bifurcated spine scales are likely an ancestral trait of this genus. Furthermore, the taxonomic status of <em>A. turfacea</em> ssp. <em>decidens</em> Nicholls, 2023 is revised and elevated to species level as <em>A. decidens</em> Nicholls, 2023 n. stat. The <em>A. turfacea</em> species complex is revisited.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"100 ","pages":"Article 126159"},"PeriodicalIF":1.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the macronuclear and micronuclear pheromone genes of Euplotes raikovi reveals the origin of the mating type genetic diversity 大核和微核信息素基因的分析揭示了交配型遗传多样性的起源

IF 1.9 2区生物学

European journal of protistology Pub Date : 2025-06-01 Epub Date: 2025-03-22 DOI: 10.1016/j.ejop.2025.126146

Jianjun Huang , Ruitao Gong , Khaled A.S. Al-Rasheid , Feng Gao , Adriana Vallesi , Yaohan Jiang

{"title":"Characterization of the macronuclear and micronuclear pheromone genes of Euplotes raikovi reveals the origin of the mating type genetic diversity","authors":"Jianjun Huang , Ruitao Gong , Khaled A.S. Al-Rasheid , Feng Gao , Adriana Vallesi , Yaohan Jiang","doi":"10.1016/j.ejop.2025.126146","DOIUrl":"10.1016/j.ejop.2025.126146","url":null,"abstract":"<div><div>Ciliates produce diffusible, cell-type-specific pheromones to regulate growth and mating. In <em>Euplotes</em>, these signaling molecules belong to species-specific families of disulfide-rich and structurally homologous proteins. Pheromones are co-dominantly expressed by genes in the somatic macronucleus (MAC), whereas their allelic diversity originates from the <em>mating type locus</em> in the germline micronucleus (MIC). During MAC development in sexual process, the MIC-derived diversity of specific alleles is rearranged via macronucleus-destined sequences (MDSs) assembly. While many MAC pheromones are well characterized, their MIC precursors and rearrangement process remain unknown. Here, we identified two MAC pheromone genes (<em>mac-er-13/14</em>) of <em>E. raikovi</em>, and two MIC regions (19 kb in total) containing 10 MDSs that assemble into <em>mac-er-13</em>. These MDSs are separated by internal eliminated sequences (234–3345 bp). The shortest MDSs (9–36 bp) encode the secreted region of pheromone, while longer MDSs (44–419 bp) encode other regions. Considering that the secreted regions show a higher sequence variation and the shorter MDSs have higher probability of alternative processing or imprecise assembly, we hypothesize that the high sequence variability of the macronuclear pheromone genes, which underlies the large number of mating types in <em>E. raikovi</em>, may result from alternative processing or imprecise assembly of these short MDSs.</div></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"99 ","pages":"Article 126146"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0