European journal of protistology最新文献_第10页

Anaerocyclidiidae fam. nov. (Oligohymenophorea, Scuticociliatia): A newly recognized major lineage of anaerobic ciliates hosting prokaryotic symbionts Anaerocyclidiidae家人。11 .(寡膜翅目，scuticocilatia):一个新认识的主要谱系的厌氧纤毛虫寄主原核共生体

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126009

Kateřina Poláková, William A. Bourland, Ivan Čepička

{"title":"Anaerocyclidiidae fam. nov. (Oligohymenophorea, Scuticociliatia): A newly recognized major lineage of anaerobic ciliates hosting prokaryotic symbionts","authors":"Kateřina Poláková, William A. Bourland, Ivan Čepička","doi":"10.1016/j.ejop.2023.126009","DOIUrl":"10.1016/j.ejop.2023.126009","url":null,"abstract":"<div>The research on anaerobic ciliates, to date, has mainly been focused on representatives of obligately anaerobic classes such as Armophorea or Plagiopylea. In this study, we focus on the anaerobic representatives of the subclass Scuticociliatia, members of the class Oligohymenophorea, which is mainly composed of aerobic ciliates. Until now, only a single anaerobic species, Cyclidium porcatum (here transferred to the genus Anaerocyclidium gen. nov.), has been described both molecularly and morphologically. Our broad sampling of anoxic sediments together with cultivation and single cell sequencing approaches have shown that scuticociliates are common and diversified in anoxic environments. Our results show that anaerobic scuticociliates represent a distinctive evolutionary lineage not closely related to the family Cyclidiidae (order Pleuronematida), as previously suggested. However, the phylogenetic position of the newly recognized lineage within the subclass Scuticociliatia remains unresolved. Based on molecular and morphological data, we establish the family Anaerocyclidiidae fam. nov. to accommodate members of this clade. We further provide detailed morphological descriptions and 18S rRNA gene sequences for six new Anaerocyclidium species and significantly broaden the described diversity of anaerobic scuticociliates.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126009"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10208440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Euglena gracilis: Biochemical properties of a membrane bound ecto-phosphatase activity modulated by fluoroaluminate complexes and different trophic conditions 薄叶草:膜结合外磷酸酶活性受氟铝酸盐复合物和不同营养条件调节的生化特性

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126010

Anita Leocadio Freitas-Mesquita , Luiz Fernando Carvalho-Kelly , Thaís Souza Silveira Majerowicz , José Roberto Meyer-Fernandes

{"title":"Euglena gracilis: Biochemical properties of a membrane bound ecto-phosphatase activity modulated by fluoroaluminate complexes and different trophic conditions","authors":"Anita Leocadio Freitas-Mesquita , Luiz Fernando Carvalho-Kelly , Thaís Souza Silveira Majerowicz , José Roberto Meyer-Fernandes","doi":"10.1016/j.ejop.2023.126010","DOIUrl":"10.1016/j.ejop.2023.126010","url":null,"abstract":"<div>The ecto-phosphatases belong to a group of enzymes closely associated with the cell surface that has its catalytic site facing the extracellular environment, where different phosphorylated substrates can be hydrolyzed. In the present work, we biochemically characterized the ecto-phosphatase activity of the freshwater microalgae Euglena gracilis, a model microorganism, ubiquitously distributed and resistant to several environmental stressors. The ecto-phosphatase activity is acidic, stimulated by copper and presents the following apparent kinetic parameters: Km = 2.52 ± 0.12 mM p-NPP and Vmax = 3.62 ± 0.06 nmol p-NP × h−1 × 106 cells. We observed that zinc, orthovanadate, molybdate, fluoride, and inorganic phosphate inhibit the ecto-phosphatase activity with different magnitudes. Fluoroaluminate complexes are also inhibitors of this ecto-phosphatase activity. They can be formed in the enzyme reaction conditions and are likely to occur in a natural environment where E. gracilis can be found. The ecto-phosphatase activity is constant through the culture growth phases and is negatively modulated after continuous subculturing in the dark when a shift from phototrophic to the heterotrophic metabolism is likely. The analysis of those biochemical parameters may contribute to understanding the role of E. gracilis ecto-phosphatase activity in natural environments.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126010"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holospora-like bacteria “Candidatus Gortzia yakutica” and Preeria caryophila: Ultrastructure, promiscuity, and biogeography of the symbionts 全息孢子样细菌" Candidatus Gortzia yakutica "和Preeria caryloila:共生体的超微结构、混杂性和生物地理学

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.125998

Sergei I. Fokin , Natalia A. Lebedeva , Alexey Potekhin , Leandro Gammuto , Giulio Petroni , Valentina Serra

{"title":"Holospora-like bacteria “Candidatus Gortzia yakutica” and Preeria caryophila: Ultrastructure, promiscuity, and biogeography of the symbionts","authors":"Sergei I. Fokin , Natalia A. Lebedeva , Alexey Potekhin , Leandro Gammuto , Giulio Petroni , Valentina Serra","doi":"10.1016/j.ejop.2023.125998","DOIUrl":"10.1016/j.ejop.2023.125998","url":null,"abstract":"<div>Two already known representatives of Holospora-like bacteria, “Candidatus Gortzia yakutica” from Paramecium putrinum and Preeria caryophila, originally retrieved from the Paramecium aurelia complex, were found in new hosts: Paramecium nephridiatum and Paramecium polycaryum, respectively. In the present study, these bacteria were investigated using morphological and molecular methods. For “Ca. G. yakutica”, the first details of the electron microscopic structure in the main and new hosts were provided. Regarding Pr. caryophila, the ultrastructural description of this species was implemented by several features previously unknown, such as the so called “membrane cluster” dividing periplasm from cytoplasm and fine composition of infectious forms before and during its releasing from the infected macronucleus. The new combinations of these Holospora-like bacteria with ciliate hosts were discussed from biogeographical and ecological points of view. Host specificity of symbionts as a general paradigm was critically reviewed as well.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 125998"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10204820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Host specificity of passerine Lankesterella (Apicomplexa: Coccidia) 球球虫(顶复合体:球虫)寄主特异性的研究

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126007

Ashwin Kumar Saravana Bhavan Venkatachalam , Ivan Čepička , Kristýna Hrazdilová , Milena Svobodová

{"title":"Host specificity of passerine Lankesterella (Apicomplexa: Coccidia)","authors":"Ashwin Kumar Saravana Bhavan Venkatachalam , Ivan Čepička , Kristýna Hrazdilová , Milena Svobodová","doi":"10.1016/j.ejop.2023.126007","DOIUrl":"10.1016/j.ejop.2023.126007","url":null,"abstract":"<div>Lankesterella parasites are blood coccidians that have recently gained attention as their records in common passerine species emerge. To date, their occurrence has been molecularly confirmed in several passerine genera, mainly among members of the families Paridae and Acrocephalidae. Despite their relatively high prevalence in some host populations, their life cycles remain unclear, mosquitoes or mites being the proposed vectors. The aim of this study was to reveal Lankesterella host specificity, focusing mainly on parasites of tit and warbler species (families Paridae and Acrocephalidae). We have determined the 18S rRNA gene sequences of Lankesterella from 35 individuals belonging to eight different host species. Phylogenetic analysis revealed that passerine Lankesterella are host-specific, with specificity at the host genus or species level. Besides Lankesterella, Isospora sequences were obtained from avian blood as well, pointing out the need for barcoding.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126007"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10209297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Morphologic and molecular characterization of Apertospathula pilata n. sp., a novel freshwater spathidiid (Ciliophora Litostomatea) from Idaho USA” [Eur. J. Protistol. 89 (2023) 125990] 来自美国爱达荷州的一种新型淡水蜘蛛(Ciliophora Litostomatea)的形态和分子特征的勘误表[欧洲]。[j]中华医学杂志。89 (2023):125990]

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126011

William Bourland

引用次数: 0

First redescription and molecular phylogeny of Trimyema claviforme Kahl, 1933 with the description of a Chinese population of Plagiopyla nasuta Stein, 1860 (Ciliophora, Plagiopylea) 棒形Trimyema claiforme Kahl的首次重新描述和分子系统发育，1933年，对中国的Plagiopyla nasuta Stein种群的描述，1860年（Ciliophora，Plagiopylea）。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126003

Ran Li , Wenbao Zhuang , Xiaochen Feng , Hunter N. Hines , Xiaozhong Hu

{"title":"First redescription and molecular phylogeny of Trimyema claviforme Kahl, 1933 with the description of a Chinese population of Plagiopyla nasuta Stein, 1860 (Ciliophora, Plagiopylea)","authors":"Ran Li , Wenbao Zhuang , Xiaochen Feng , Hunter N. Hines , Xiaozhong Hu","doi":"10.1016/j.ejop.2023.126003","DOIUrl":"10.1016/j.ejop.2023.126003","url":null,"abstract":"<div>Ciliates belonging to the class Plagiopylea are obligate anaerobes that are often neglected due to their cryptic lifestyles, difficulty of observation, and overall under-sampling. Here, we investigate two species, namely Trimyema claviforme Kahl, 1933 and Plagiopyla nasuta Stein, 1860, collected in China from marine and freshwater anaerobic sediments, respectively. A complete morphological dataset, together with SSU rRNA gene sequence data were obtained and used to diagnose the species. No molecular sequencing had ever been performed on Trimyema claviforme, with its ciliature also previously unknown. Based on these novel data presented here, the ciliate is characterized by a claviform cell shape, with a size of 35–45 × 10–20 μm in vivo, 28–39 longitudinal somatic ciliary rows forming five ciliary girdles (four complete girdles and a shorter one), two dikinetids left to anterior end of oral kinety 1, and an epaulet. A Chinese population of the well-known ciliate P. nasuta was investigated, and morphological comparisons revealed phenotypic stability of the species. The phylogenetic analyses supported previous findings about the monophyly of the families Trimyemidae and Plagiopylidae, with Trimyema claviforme branching off early in the genus Trimyema. The Chinese population of P. nasuta clusters together with two other populations with full support corroborating their conspecificity.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126003"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10208686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ciliates from the intestine of zoo-kept black rhinoceros, with immunofluorescence microscopic and molecular phylogenetic investigation of Rhinozeta rhinozeta (Litostomatea) 动物园饲养黑犀牛肠道纤毛的免疫荧光显微镜观察与分子系统发育研究

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126006

Olga A. Kornilova , Maria E. Belokon , Maria A. Skazina , Olga S. Alekseeva , Ludmila V. Chistyakova

{"title":"Ciliates from the intestine of zoo-kept black rhinoceros, with immunofluorescence microscopic and molecular phylogenetic investigation of Rhinozeta rhinozeta (Litostomatea)","authors":"Olga A. Kornilova , Maria E. Belokon , Maria A. Skazina , Olga S. Alekseeva , Ludmila V. Chistyakova","doi":"10.1016/j.ejop.2023.126006","DOIUrl":"10.1016/j.ejop.2023.126006","url":null,"abstract":"<div>The study of endobiotic ciliates from the intestines of various zoo mammals is important for revealing the influence of various factors on endobiont communities. In this paper we describe the species diversity of endobiotic ciliates from the faeces of the eastern black rhinoceros Diceros bicornis michaeli (male and two females, mother and daughter) kept in the zoo. Seven species of the ciliates were found, among them Rhinozeta rhinozeta, R. triciliata and Prototapirella clypeata were observed in the rhinos only. The other two, Monoposthium sp. and Triplumaria sp., according to their morphology should have been identified as new ciliate species. Successful transmission of the endobionts from parents to the young rhino in the zoo was demonstrated. R. rhinozeta was investigated with the using of the methods of the light and immunofluorescence microscopy and molecular phylogeny. The composition and phylogeny of the family Cycloposthiidae were discussed.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126006"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10210751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gregarines from darkling beetles of the Atacama Desert, Atacamagregarina paposa gen. et sp. nov. from Scotobius and Xiphocephalus ovatus sp. nov. from Psectrascelis (Coleoptera, Tenebrionidae) 阿塔卡马沙漠深色甲虫的灰蝶，Scotobius的阿塔卡马灰蝶gen.et sp.nov.和Psectrascelis的卵圆犀（鞘翅目，Tenebrionidae）。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126008

Frank Nitsche , Sarah Carduck , Joshua von Ameln , Niclas Mach , Cristina Dorador , Reinhard Predel , Sonja Rueckert , Hartmut Arndt

{"title":"Gregarines from darkling beetles of the Atacama Desert, Atacamagregarina paposa gen. et sp. nov. from Scotobius and Xiphocephalus ovatus sp. nov. from Psectrascelis (Coleoptera, Tenebrionidae)","authors":"Frank Nitsche , Sarah Carduck , Joshua von Ameln , Niclas Mach , Cristina Dorador , Reinhard Predel , Sonja Rueckert , Hartmut Arndt","doi":"10.1016/j.ejop.2023.126008","DOIUrl":"10.1016/j.ejop.2023.126008","url":null,"abstract":"<div>Gregarine apicomplexans, a group of single celled organisms, inhabit the extracellular spaces of most invertebrate species. The nature of the gregarine-host interactions is not yet fully resolved, mutualistic, commensal and parasitic life forms have been recorded. In the extreme arid environment of the Atacama Desert, only a few groups of invertebrates hosting gregarines such as darkling beetles (Tenebrionidae) were able to adapt, providing an unparalleled opportunity to study co-evolutionary diversification. Here, we describe one novel gregarine genus comprising one species, Atacamagregarina paposa gen. et sp. nov., and a new species, Xiphocephalus ovatus sp. nov. (Apicomplexa: Eugregarinoridea, Stylocephalidae), found in the tenebrionid beetle genera Scotobius (Tenebrioninae, Scotobiini) and Psectrascelis intricaticollis ovata (Pimeliinae, Nycteliini), respectively. In the phylogenetic analysis based on SSU rDNA, Atacamgregarina paposa representing the new genus is basal, forming a separate clade with terrestrial gregarines specific for North American darkling beetles.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126008"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10211239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel contributions to the molecular and morphological diversity of freshwater monothalamid foraminifera: Description of six new species 淡水单丘脑有孔虫分子和形态多样性的新贡献:六个新种的描述

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126014

Ferry Siemensma , Maria Holzmann

{"title":"Novel contributions to the molecular and morphological diversity of freshwater monothalamid foraminifera: Description of six new species","authors":"Ferry Siemensma , Maria Holzmann","doi":"10.1016/j.ejop.2023.126014","DOIUrl":"10.1016/j.ejop.2023.126014","url":null,"abstract":"<div>Non-marine monothalamous foraminifera are common in freshwater and soil habitats. They comprise a poorly-known group lacking sufficient information about diversity, morphology, reproduction, distribution and ecology. Based on an integrative morphological and molecular approach we describe a new family, a new genus and six new species of freshwater monothalamids from different localities in the Netherlands and France. We establish Astroperula as a new genus of organic walled freshwater foraminifera that contains two species, Astroperula dumacki and Astroperula parvipila. Furthermore, two new agglutinated freshwater monothalamids are described and illustrated, Limnogromia leanneae and Lacogromia pawlowskii, one new organic walled, Velamentofex dujardini, and a new naked monothalamid, Haplomyxa retiforma. Additional information is provided about a special form of cell division in Claparedellus lachmannii and L. leanneae, and feeding behavior in cultures of V. dujardini. Morphological observations are added for an unidentified Limnogromia sampled from the Netherlands and two types of Lacogromia sampled from an alpine region in the French Pyrenees and a karst sinkhole in Bosnia and Herzegovina. The present study provides additional insight into the ecology, diversity and occurrence of freshwater monothalamids and emphasizes the fact that a combination of morphological and molecular methods is necessary to clearly distinguish species in this group.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126014"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10210338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome size and GC content of myxomycetes 黏菌的基因组大小和GC含量

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.125991

Shu Li , Bao Qi , Xueyan Peng , Wei Wang , Wan Wang , Pu Liu , Bao Liu , Zhanwu Peng , Qi Wang , Yu Li

引用次数: 0