European journal of protistology最新文献_第9页

Ciliates from the intestine of zoo-kept black rhinoceros, with immunofluorescence microscopic and molecular phylogenetic investigation of Rhinozeta rhinozeta (Litostomatea) 动物园饲养黑犀牛肠道纤毛的免疫荧光显微镜观察与分子系统发育研究

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126006

Olga A. Kornilova , Maria E. Belokon , Maria A. Skazina , Olga S. Alekseeva , Ludmila V. Chistyakova

{"title":"Ciliates from the intestine of zoo-kept black rhinoceros, with immunofluorescence microscopic and molecular phylogenetic investigation of Rhinozeta rhinozeta (Litostomatea)","authors":"Olga A. Kornilova , Maria E. Belokon , Maria A. Skazina , Olga S. Alekseeva , Ludmila V. Chistyakova","doi":"10.1016/j.ejop.2023.126006","DOIUrl":"10.1016/j.ejop.2023.126006","url":null,"abstract":"<div>The study of endobiotic ciliates from the intestines of various zoo mammals is important for revealing the influence of various factors on endobiont communities. In this paper we describe the species diversity of endobiotic ciliates from the faeces of the eastern black rhinoceros Diceros bicornis michaeli (male and two females, mother and daughter) kept in the zoo. Seven species of the ciliates were found, among them Rhinozeta rhinozeta, R. triciliata and Prototapirella clypeata were observed in the rhinos only. The other two, Monoposthium sp. and Triplumaria sp., according to their morphology should have been identified as new ciliate species. Successful transmission of the endobionts from parents to the young rhino in the zoo was demonstrated. R. rhinozeta was investigated with the using of the methods of the light and immunofluorescence microscopy and molecular phylogeny. The composition and phylogeny of the family Cycloposthiidae were discussed.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126006"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10210751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gregarines from darkling beetles of the Atacama Desert, Atacamagregarina paposa gen. et sp. nov. from Scotobius and Xiphocephalus ovatus sp. nov. from Psectrascelis (Coleoptera, Tenebrionidae) 阿塔卡马沙漠深色甲虫的灰蝶，Scotobius的阿塔卡马灰蝶gen.et sp.nov.和Psectrascelis的卵圆犀（鞘翅目，Tenebrionidae）。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126008

Frank Nitsche , Sarah Carduck , Joshua von Ameln , Niclas Mach , Cristina Dorador , Reinhard Predel , Sonja Rueckert , Hartmut Arndt

{"title":"Gregarines from darkling beetles of the Atacama Desert, Atacamagregarina paposa gen. et sp. nov. from Scotobius and Xiphocephalus ovatus sp. nov. from Psectrascelis (Coleoptera, Tenebrionidae)","authors":"Frank Nitsche , Sarah Carduck , Joshua von Ameln , Niclas Mach , Cristina Dorador , Reinhard Predel , Sonja Rueckert , Hartmut Arndt","doi":"10.1016/j.ejop.2023.126008","DOIUrl":"10.1016/j.ejop.2023.126008","url":null,"abstract":"<div>Gregarine apicomplexans, a group of single celled organisms, inhabit the extracellular spaces of most invertebrate species. The nature of the gregarine-host interactions is not yet fully resolved, mutualistic, commensal and parasitic life forms have been recorded. In the extreme arid environment of the Atacama Desert, only a few groups of invertebrates hosting gregarines such as darkling beetles (Tenebrionidae) were able to adapt, providing an unparalleled opportunity to study co-evolutionary diversification. Here, we describe one novel gregarine genus comprising one species, Atacamagregarina paposa gen. et sp. nov., and a new species, Xiphocephalus ovatus sp. nov. (Apicomplexa: Eugregarinoridea, Stylocephalidae), found in the tenebrionid beetle genera Scotobius (Tenebrioninae, Scotobiini) and Psectrascelis intricaticollis ovata (Pimeliinae, Nycteliini), respectively. In the phylogenetic analysis based on SSU rDNA, Atacamgregarina paposa representing the new genus is basal, forming a separate clade with terrestrial gregarines specific for North American darkling beetles.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126008"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10211239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel contributions to the molecular and morphological diversity of freshwater monothalamid foraminifera: Description of six new species 淡水单丘脑有孔虫分子和形态多样性的新贡献:六个新种的描述

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.126014

Ferry Siemensma , Maria Holzmann

{"title":"Novel contributions to the molecular and morphological diversity of freshwater monothalamid foraminifera: Description of six new species","authors":"Ferry Siemensma , Maria Holzmann","doi":"10.1016/j.ejop.2023.126014","DOIUrl":"10.1016/j.ejop.2023.126014","url":null,"abstract":"<div>Non-marine monothalamous foraminifera are common in freshwater and soil habitats. They comprise a poorly-known group lacking sufficient information about diversity, morphology, reproduction, distribution and ecology. Based on an integrative morphological and molecular approach we describe a new family, a new genus and six new species of freshwater monothalamids from different localities in the Netherlands and France. We establish Astroperula as a new genus of organic walled freshwater foraminifera that contains two species, Astroperula dumacki and Astroperula parvipila. Furthermore, two new agglutinated freshwater monothalamids are described and illustrated, Limnogromia leanneae and Lacogromia pawlowskii, one new organic walled, Velamentofex dujardini, and a new naked monothalamid, Haplomyxa retiforma. Additional information is provided about a special form of cell division in Claparedellus lachmannii and L. leanneae, and feeding behavior in cultures of V. dujardini. Morphological observations are added for an unidentified Limnogromia sampled from the Netherlands and two types of Lacogromia sampled from an alpine region in the French Pyrenees and a karst sinkhole in Bosnia and Herzegovina. The present study provides additional insight into the ecology, diversity and occurrence of freshwater monothalamids and emphasizes the fact that a combination of morphological and molecular methods is necessary to clearly distinguish species in this group.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 126014"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10210338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome size and GC content of myxomycetes 黏菌的基因组大小和GC含量

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.125991

Shu Li , Bao Qi , Xueyan Peng , Wei Wang , Wan Wang , Pu Liu , Bao Liu , Zhanwu Peng , Qi Wang , Yu Li

引用次数: 0

Identification of the heterotrophic nanoflagellate Bilabrum latius in the southern Adriatic (Mediterranean Sea) 南亚得里亚海(地中海)异养型纳米鞭毛虫的鉴定

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-08-01 DOI: 10.1016/j.ejop.2023.125999

Ana Baricevic, Daniela Maric Pfannkuchen, Mirta Smodlaka Tankovic, Mia Knjaz, Ivan Vlasicek, Lana Grizancic, Tjasa Kogovsek, Martin Pfannkuchen

{"title":"Identification of the heterotrophic nanoflagellate Bilabrum latius in the southern Adriatic (Mediterranean Sea)","authors":"Ana Baricevic, Daniela Maric Pfannkuchen, Mirta Smodlaka Tankovic, Mia Knjaz, Ivan Vlasicek, Lana Grizancic, Tjasa Kogovsek, Martin Pfannkuchen","doi":"10.1016/j.ejop.2023.125999","DOIUrl":"10.1016/j.ejop.2023.125999","url":null,"abstract":"<div>Heterotrophic flagellates (HF) represent an important protist group in marine ecosystem functioning. Characterised by high taxonomic diversity, identification and classification of HF is often difficult using classical methods of light microscopy (LM). Complementing LM with molecular methods, such as barcoding, enables reliable taxonomic identification of even small size nanoflagellates that share similar or unnoticeable morphological features. The order Bicosoecida is a group of heterotrophic nanoflagellates that are important part of protist plankton and benthic communities of the world oceans. Recently, on the basis of high-resolution light microscopy and barcoding, a new bicosoecid genus, Bilabrum, was described with B. latius sp. as a type species. Our study reports on identification of B. latius from co-culture with the diatom species Chaetoceros affinis isolated from fresh plankton samples collected in the southern Adriatic. This detection of the Adriatic B.latius represents first record of this species outside itś up to now known and described habitat (deep-sea sediment of the South - East Atlantic Ocean) and in diatom co-culture.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"90 ","pages":"Article 125999"},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10207639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptome reveals cell division-regulated hub genes in the unicellular eukaryote Paramecium 单细胞转录组揭示了单细胞真核生物草履虫中细胞分裂调控的枢纽基因

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125978

Juan Yang , Zhenyuan Wang , Chundi Wang , Danxu Tang , Zihan Zang , Naomi A. Stover , Xiao Chen , Lifang Li

{"title":"Single-cell transcriptome reveals cell division-regulated hub genes in the unicellular eukaryote Paramecium","authors":"Juan Yang , Zhenyuan Wang , Chundi Wang , Danxu Tang , Zihan Zang , Naomi A. Stover , Xiao Chen , Lifang Li","doi":"10.1016/j.ejop.2023.125978","DOIUrl":"10.1016/j.ejop.2023.125978","url":null,"abstract":"<div>The transition from growth to division during the cell cycle encompasses numerous conserved processes such as large-scale DNA replication and protein synthesis. In ciliate cells, asexual cell division is accompanied by additional cellular changes including amitotic nuclear division, extensive ciliogenesis, and trichocyst replication. However, the molecular mechanisms underlying these processes remain elusive. In this study, we present single-cell gene expression profiles of Paramecium cf. multimicronucleatum cells undergoing cell division. Our results reveal that the most up-regulated genes in dividing cells compared to growing cells are associated with 1) cell cycle signaling pathways including transcription, DNA replication, chromosome segregation and protein degradation; 2) microtubule proteins and tubulin glycylases which are essential for ciliogenesis, nuclei separation and structural differentiation signaling; and 3) trichocyst matrix proteins involved in trichocyst synthesis and reproduction. Furthermore, weighted gene co-expression network analysis identified hub genes that may play crucial roles during cell division. Our findings provide insights into cell cycle regulators, microtubules and trichocyst matrix proteins that may exert influence on this process in ciliates.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"89 ","pages":"Article 125978"},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9647201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphologic and molecular characterization of Apertospathula pilata n. sp., a novel freshwater spathidiid (Ciliophora, Litostomatea) from Idaho, USA 美国爱达荷州一种新型淡水蜘蛛(纤毛虫科，Litostomatea)的形态和分子特征

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125990

William Bourland

{"title":"Morphologic and molecular characterization of Apertospathula pilata n. sp., a novel freshwater spathidiid (Ciliophora, Litostomatea) from Idaho, USA","authors":"William Bourland","doi":"10.1016/j.ejop.2023.125990","DOIUrl":"10.1016/j.ejop.2023.125990","url":null,"abstract":"<div>Order Spathidiida Foissner and Foissner, 1988 comprises a large group of morphologically diverse, primarily predatory, free living ciliates, the phylogeny of which has remained stubbornly unresolved. Families Arcuospathidiidae and Apertospathulidae are two morphologically similar groups established on the basis of differences in the morphology of the oral bulge and circumoral kinety. While Arcuospathidiidae is non-monophyletic in 18S rRNA gene analyses, the Apertospathulidae has been represented by only a single Apertospathula sequence in public databases. In this report, a novel freshwater species, Apertospathula pilata n. sp. is described on the basis of living observation, silver impregnation, and scanning electron microscopy. The phylogeny of the new species is assessed based on the rRNA cistron. The main features distinguishing A. pilata n. sp. from all congeners are: the oral bulge extrusomes (filiform, up to 25 µm long), the combination of body size (130–193 µm) and shape (spatulate), the extensive oral bulge length (41% of the cell length after protargol impregnation), and multiple micronuclei (one to five, two on average). The monophyly of Apertospathulidae Foissner, Xu and Kreutz, 2005 is rejected.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"89 ","pages":"Article 125990"},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9864006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two new Raabena species and a new Pararaabena species (Ciliophora, Entodiniomorphida) with redescriptions of Raabena bella and Pararaabena dentata 二新种和一新种(纤毛虫目，内二形目)，并重新描述了贝拉和齿形旁贝拉

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125986

Akira Ito

{"title":"Two new Raabena species and a new Pararaabena species (Ciliophora, Entodiniomorphida) with redescriptions of Raabena bella and Pararaabena dentata","authors":"Akira Ito","doi":"10.1016/j.ejop.2023.125986","DOIUrl":"10.1016/j.ejop.2023.125986","url":null,"abstract":"<div>The genera Raabena and Pararaabena (Ciliophora, Entodiniomorphida, Blepharocorythidae) were monospecific, and their type species are Raabena bella Wolska, 1967 and Pararaabena dentata Wolska, 1968. They have been found in Asian elephants and closely resemble each other: ovoid and laterally compressed body; non-retractable adoral ciliary zone; funnel-shaped vestibulum; three non-retractable somatic ciliary arches. Furthermore, the positional relationship between the vestibular ciliary zone and the anterior dorsal ciliary zone identifies Raabena and Pararaabena: these two ciliary zones are connected in Raabena while they are separated in Pararaabena. While investigating entodiniomorphid ciliates of Asian elephants, the author often encountered ciliates similar to Raabena bella but with a sinuous body or with a small body and ciliates similar to Pararaabena dentata but with a slender body or with no or two caudal lobes. In this study, their general morphology and infraciliature were compared to R. bella and P. dentata to know whether they are new species or morphological variations in a species. As a result, the present study redescribed R. bella and P. dentata, and described R. sinuosa n. sp., R. bellafilia n. sp., P. gracilis n. sp., and morphotypes of P. dentata.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"89 ","pages":"Article 125986"},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testate amoebae response and vegetation composition after plantation removal on a former raised bog 在前抬高的沼泽上移除人工林后，遗存变形虫的反应和植被组成

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125977

Angela L. Creevy , David M. Wilkinson , Roxane Andersen , Richard J. Payne

{"title":"Testate amoebae response and vegetation composition after plantation removal on a former raised bog","authors":"Angela L. Creevy , David M. Wilkinson , Roxane Andersen , Richard J. Payne","doi":"10.1016/j.ejop.2023.125977","DOIUrl":"10.1016/j.ejop.2023.125977","url":null,"abstract":"<div>Extensive drainage of peatlands in north-west Europe for the purposes of afforestation for timber production and harvesting has altered the carbon balance and biodiversity value. Large-scale restoration projects aim to reinstate hydrological conditions to keep carbon locked up in the peat and to restart active peat growth. Testate amoebae are an informal grouping of well-studied protists in peatland environments and as microbial consumers play an important role in nutrient and carbon cycling. Using a space for time substitution approach, this study investigated the response of testate amoebae assemblages and vegetation composition after tree removal on a drained raised bog. There was a clear difference in microbial assemblages between open and a chronosequence of restoration areas. Results suggest microbial recovery after rewetting is a slow process with plant composition showing a faster response than the microbial assemblage. Mixotrophic testate amoebae had not recovered seventeen years following plantation removal and the establishment of Sphagnum mosses in the wetter microforms. These results suggest that vegetation composition and Testate amoeba assemblages respond differently to environmental drivers at forest-to-bog restoration areas. Local physicochemical peat properties were a stronger driver of the testate assemblage compared with vegetation. Complete recovery of microbial assemblages may take place over decadal timescales.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"89 ","pages":"Article 125977"},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9703381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific island of Okinawa, Japan 来自日本冲绳岛太平洋上的虾链藻(Dinophyceae)的细菌群落和毒素谱

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125976

Yong Heng Phua , Javier Tejeda , Michael C. Roy , Filip Husnik , Kevin C. Wakeman

{"title":"Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific island of Okinawa, Japan","authors":"Yong Heng Phua , Javier Tejeda , Michael C. Roy , Filip Husnik , Kevin C. Wakeman","doi":"10.1016/j.ejop.2023.125976","DOIUrl":"10.1016/j.ejop.2023.125976","url":null,"abstract":"<div>Variations in toxicity of the benthic dinoflagellate Ostreopsis Schmidt 1901 have been attributed to specific molecular clades, biogeography of isolated strains, and the associated bacterial community. Here, we attempted to better understand the biodiversity and the basic biology influencing toxin production of Ostreopsis. Nine clonal cultures were established from Okinawa, Japan, and identified using phylogenetic analysis of the ITS-5.8S rRNA and 28S rRNA genes. Morphological analysis suggests that the apical pore complex L/W ratio could be a feature for differentiating Ostreopsis sp. 2 from the O. ovata species complex. We analyzed the toxicity and bacterial communities using liquid chromatography-mass spectrometry, and PCR-free metagenomic sequencing. Ovatoxin was detected in three of the seven strains of O. cf. ovata extracts, highlighting intraspecies variation in toxin production. Additionally, two new potential analogs of ovatoxin-a and ostreocin-A were identified. Commonly associated bacteria clades of Ostreopsis were identified from the established cultures. While some of these bacteria groups may be common to Ostreopsis (Rhodobacterales, Flavobacteria-Sphingobacteria, and Enterobacterales), it was not clear from our analysis if any one or more of these plays a role in toxin biosynthesis. Further examination of biosynthetic pathways in metagenomic data and additional experiments isolating specific bacteria from Ostreopsis would aid these efforts.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"89 ","pages":"Article 125976"},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9703382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0