European journal of protistology最新文献_第9页

Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific island of Okinawa, Japan 来自日本冲绳岛太平洋上的虾链藻(Dinophyceae)的细菌群落和毒素谱

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125976

Yong Heng Phua , Javier Tejeda , Michael C. Roy , Filip Husnik , Kevin C. Wakeman

{"title":"Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific island of Okinawa, Japan","authors":"Yong Heng Phua , Javier Tejeda , Michael C. Roy , Filip Husnik , Kevin C. Wakeman","doi":"10.1016/j.ejop.2023.125976","DOIUrl":"10.1016/j.ejop.2023.125976","url":null,"abstract":"<div>Variations in toxicity of the benthic dinoflagellate Ostreopsis Schmidt 1901 have been attributed to specific molecular clades, biogeography of isolated strains, and the associated bacterial community. Here, we attempted to better understand the biodiversity and the basic biology influencing toxin production of Ostreopsis. Nine clonal cultures were established from Okinawa, Japan, and identified using phylogenetic analysis of the ITS-5.8S rRNA and 28S rRNA genes. Morphological analysis suggests that the apical pore complex L/W ratio could be a feature for differentiating Ostreopsis sp. 2 from the O. ovata species complex. We analyzed the toxicity and bacterial communities using liquid chromatography-mass spectrometry, and PCR-free metagenomic sequencing. Ovatoxin was detected in three of the seven strains of O. cf. ovata extracts, highlighting intraspecies variation in toxin production. Additionally, two new potential analogs of ovatoxin-a and ostreocin-A were identified. Commonly associated bacteria clades of Ostreopsis were identified from the established cultures. While some of these bacteria groups may be common to Ostreopsis (Rhodobacterales, Flavobacteria-Sphingobacteria, and Enterobacterales), it was not clear from our analysis if any one or more of these plays a role in toxin biosynthesis. Further examination of biosynthetic pathways in metagenomic data and additional experiments isolating specific bacteria from Ostreopsis would aid these efforts.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9703382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two new Raabena species and a new Pararaabena species (Ciliophora, Entodiniomorphida) with redescriptions of Raabena bella and Pararaabena dentata 二新种和一新种(纤毛虫目，内二形目)，并重新描述了贝拉和齿形旁贝拉

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125986

Akira Ito

{"title":"Two new Raabena species and a new Pararaabena species (Ciliophora, Entodiniomorphida) with redescriptions of Raabena bella and Pararaabena dentata","authors":"Akira Ito","doi":"10.1016/j.ejop.2023.125986","DOIUrl":"10.1016/j.ejop.2023.125986","url":null,"abstract":"<div>The genera Raabena and Pararaabena (Ciliophora, Entodiniomorphida, Blepharocorythidae) were monospecific, and their type species are Raabena bella Wolska, 1967 and Pararaabena dentata Wolska, 1968. They have been found in Asian elephants and closely resemble each other: ovoid and laterally compressed body; non-retractable adoral ciliary zone; funnel-shaped vestibulum; three non-retractable somatic ciliary arches. Furthermore, the positional relationship between the vestibular ciliary zone and the anterior dorsal ciliary zone identifies Raabena and Pararaabena: these two ciliary zones are connected in Raabena while they are separated in Pararaabena. While investigating entodiniomorphid ciliates of Asian elephants, the author often encountered ciliates similar to Raabena bella but with a sinuous body or with a small body and ciliates similar to Pararaabena dentata but with a slender body or with no or two caudal lobes. In this study, their general morphology and infraciliature were compared to R. bella and P. dentata to know whether they are new species or morphological variations in a species. As a result, the present study redescribed R. bella and P. dentata, and described R. sinuosa n. sp., R. bellafilia n. sp., P. gracilis n. sp., and morphotypes of P. dentata.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High diversity and isolated distribution of aquatic heterotrophic protists in salars of the Atacama Desert at different salinities 阿塔卡马沙漠不同盐度萨拉尔水生异养原生生物的高度多样性和孤立分布

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125987

Alexandra E. Lennartz (née Rybarski) , Frank Nitsche , Alexandra Schoenle , Claudia Voigt , Michael Staubwasser , Hartmut Arndt

{"title":"High diversity and isolated distribution of aquatic heterotrophic protists in salars of the Atacama Desert at different salinities","authors":"Alexandra E. Lennartz (née Rybarski) , Frank Nitsche , Alexandra Schoenle , Claudia Voigt , Michael Staubwasser , Hartmut Arndt","doi":"10.1016/j.ejop.2023.125987","DOIUrl":"10.1016/j.ejop.2023.125987","url":null,"abstract":"<div>The species richness of eukaryotes in the hypersaline environment is generally thought to be low. However, recent studies showed a high degree of phylogenetic novelty at these extreme conditions with variable chemical parameters. These findings call for a more thorough look into the species richness of hypersaline environments. In this study, various hypersaline lakes (salars, 1–348 PSU) as well as further aquatic ecosystems of northern Chile were investigated regarding diversity of heterotrophic protists by metabarcoding studies of surface water samples. Investigations of genotypes of 18S rRNA genes showed a unique community composition in nearly each salar and even among different microhabitats within one salar. The genotype distribution showed no clear connection to the composition of main ions at the sampling sites, but protist communities from similar salinity ranges (either hypersaline, hyposaline or mesosaline) clustered together regarding their OTU composition. Salars appeared to be fairly isolated systems with only little exchange of protist communities where evolutionary lineages could separately evolve.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10025414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Tardigrade stowaways: Literature review of Propyxidium tardigradum (Ciliophora, Peritrichia) and its first record in Scotland 缓步动物偷渡者:文献综述缓步丙基(Ciliophora, Peritrichia)及其在苏格兰的首次记录

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-06-01 DOI: 10.1016/j.ejop.2023.125974

Karol Wałach , Brian Blagden

{"title":"Tardigrade stowaways: Literature review of Propyxidium tardigradum (Ciliophora, Peritrichia) and its first record in Scotland","authors":"Karol Wałach , Brian Blagden","doi":"10.1016/j.ejop.2023.125974","DOIUrl":"10.1016/j.ejop.2023.125974","url":null,"abstract":"<div>Tardigrades are a phylum of microscopic invertebrates with a global distribution. Although our understanding of their systematic position and taxonomy has increased and continues to grow, their relationship with the other organisms that share their habitat remains poorly studied. One such organism is Propyxidium tardigradum, a peritrich ciliate which uses tardigrades for dispersion and as a substrate for reproduction. Here, we present the first Scottish record and tenth global occurrence of Propyxidium tardigradum, thereby expanding our knowledge of its poorly understood zoogeographic distribution. We also summarise the literature concerning P. tardigradum biology, proffer hypotheses regarding the Propyxidium-tardigrade relationship, and the apparent lack of heterotardigrade ciliate infestation. Additionally, we indicate a number of recommendations for the direction of future studies regarding the ciliate. Finally, we add a further three species, Milnesium variefidum, Hypsibius cf. scabropygus and Macrobiotus scoticus to the list of Propyxidium host species.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10005326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphologic and molecular characterization of Apertospathula pilata n. sp., a novel freshwater spathidiid (Ciliophora, Litostomatea) from Idaho, USA. 美国爱达荷州一种新型淡水蜘蛛(纤毛虫科，Litostomatea)的形态和分子特征。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.2139/ssrn.4363217

W. Bourland

{"title":"Morphologic and molecular characterization of Apertospathula pilata n. sp., a novel freshwater spathidiid (Ciliophora, Litostomatea) from Idaho, USA.","authors":"W. Bourland","doi":"10.2139/ssrn.4363217","DOIUrl":"https://doi.org/10.2139/ssrn.4363217","url":null,"abstract":"Order Spathidiida Foissner and Foissner, 1988 comprises a large group of morphologically diverse, primarily predatory, free living ciliates, the phylogeny of which has remained stubbornly unresolved. Families Arcuospathidiidae and Apertospathulidae are two morphologically similar groups established on the basis of differences in the morphology of the oral bulge and circumoral kinety. While Arcuospathidiidae is non-monophyletic in 18S rRNA gene analyses, the Apertospathulidae has been represented by only a single Apertospathula sequence in public databases. In this report, a novel freshwater species, Apertospathula pilata n. sp. is described on the basis of living observation, silver impregnation, and scanning electron microscopy. The phylogeny of the new species is assessed based on the rRNA cistron. The main features distinguishing A. pilata n. sp. from all congeners are: the oral bulge extrusomes (filiform, up to 25 µm long), the combination of body size (130-193 µm) and shape (spatulate), the extensive oral bulge length (41% of the cell length after protargol impregnation), and multiple micronuclei (one to five, two on average). The monophyly of Apertospathulidae Foissner, Xu and Kreutz, 2005 is rejected.","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47785040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Siliceous scales in the centrohelid heliozoan Raphidocystis contractilis facilitate settlement to the substratum 具收缩性拉斐尔囊虫中心体中的硅鳞片有助于沉降到底层

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.1016/j.ejop.2023.125971

Yumeng Wan , Mikihiko Arikawa , Akane Chihara , Toshinobu Suzaki

{"title":"Siliceous scales in the centrohelid heliozoan Raphidocystis contractilis facilitate settlement to the substratum","authors":"Yumeng Wan , Mikihiko Arikawa , Akane Chihara , Toshinobu Suzaki","doi":"10.1016/j.ejop.2023.125971","DOIUrl":"10.1016/j.ejop.2023.125971","url":null,"abstract":"<div>The centrohelid heliozoan Raphidocystis contractilis has hundreds of small scales on the surface of the cell body. To understand the biological functions of the scales, comparative examinations were conducted between wild-type and scale-deficient strains that has naturally lost scales after long-term cultivation. The scale-deficient strain exhibited decreased adhesion to the substratum and had a lower sedimentation rate in water than the wild-type strain, suggesting that the scale may have the ability to attach quickly and strongly to the substratum. Percoll density gradient centrifugation showed that the scale-deficient strain had a lower density than that of the wild-type strain. In the wild-type strain, more scaled cells were observed in the higher specific gravity fractions. During the long-term culture of cells, only the cells suspended in the upper area of the flask were transferred to fresh medium. By repeating this procedure, we may have selected only cells that did not possess normal scales. In the natural environment, centrohelid heliozoans are easily flushed away if they cannot adhere strongly to the bottom. These results suggest that they use scales to ensure effective adhesion to the substratum.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9368900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphology, morphogenesis and molecular phylogeny of a novel saline soil ciliate, Urosoma quadrinucleatum n. sp. (Ciliophora, Hypotrichia) 一种新的盐渍土纤毛虫四核虫的形态、形态发生和分子系统发育

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.1016/j.ejop.2023.125970

Chen Shao , Jing Lyu , Tongxuan Li, Jingyi Wang, Chunyu Lian

{"title":"Morphology, morphogenesis and molecular phylogeny of a novel saline soil ciliate, Urosoma quadrinucleatum n. sp. (Ciliophora, Hypotrichia)","authors":"Chen Shao , Jing Lyu , Tongxuan Li, Jingyi Wang, Chunyu Lian","doi":"10.1016/j.ejop.2023.125970","DOIUrl":"10.1016/j.ejop.2023.125970","url":null,"abstract":"<div>The morphology and morphogenesis of a new saline soil hypotrichous ciliate, Urosoma quadrinucleatum n. sp., collected from northwestern China, were studied based on live observations and protargol stained specimens. The new species is characterized as follows: size in vivo 90–130 × 20–30 μm; body outline elongate-elliptical with both ends broadly rounded; four macronuclear nodules; cortical granules present; paroral in front of endoral; usually 16 frontal-ventral-transverse cirri. Urosoma quadrinucleatum n. sp. has almost the same morphogenetic characteristics as its congeners U. gigantea and U. salmastra, and differs from other three congeners whose morphogenesis is known in the formation of the frontal-ventral-transverse cirral anlagen as well as the development of marginal and dorsal kineties anlagen. The sequence differences among U. quadrinucleatum n. sp. and other Urosoma species further support the validity of the present organism as novel species. Further, U. quadrinucleatum n. sp. clusters with U. salmastra in the phylogenetic analyses based on small subunit ribosomal RNA gene (SSU rDNA) sequence data.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9683694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphological and molecular analysis of Aggregata aspera n. sp. (Apicomplexa: Aggregatidae) in Amphioctopus ovulum and Amphioctopus marginatus (Mollusca: Cephalopoda) from the Western Pacific Ocean 西太平洋两栖章鱼卵和边缘两栖章鱼（软体动物纲：头足目）Aggregata aspera n.sp.（Apicompleta：Aggregatidae）的形态和分子分析

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.1016/j.ejop.2023.125957

Lihua Wang , Jing Ren , Xiaodong Zheng

{"title":"Morphological and molecular analysis of Aggregata aspera n. sp. (Apicomplexa: Aggregatidae) in Amphioctopus ovulum and Amphioctopus marginatus (Mollusca: Cephalopoda) from the Western Pacific Ocean","authors":"Lihua Wang , Jing Ren , Xiaodong Zheng","doi":"10.1016/j.ejop.2023.125957","DOIUrl":"10.1016/j.ejop.2023.125957","url":null,"abstract":"<div>Aggregata Frenzel, 1885 (Apicomplexa) are dangerous protozoan parasites that cause malabsorption syndrome in wild and reared cephalopod species, resulting in significant economic loss to fishery and aquaculture industries. The new parasitic species, Aggregata aspera n. sp., in the digestive tract of Amphioctopus ovulum and Amphioctopus marginatus from an area in the Western Pacific Ocean was identified, it is the second two-host parasite species of Aggregata. Mature oocysts and sporocysts were spherical to ovoid in shape. Sporulated oocysts were 380.6–1,158.4 μm in length and 284.0–1,090.6 μm in width. The mature sporocysts were 16.2–18.3 μm in length and 15.7–17.6 μm in width, with irregular protuberances on the lateral wall of the sporocysts. Sporozoites within mature sporocysts were curled in shape and measured 13.0–17.0 μm in length and 1.6–2.4 μm in width. Each sporocyst contained 12–16 sporozoites. Phylogenetic tree analysis, based on 18S rRNA gene partial sequences, indicated that Ag. aspera forms a monophyletic cluster within the genus Aggregata and has a sister relationship with Ag. sinensis. These findings will provide the theoretical basis for the histopathology and diagnosis of coccidiosis in cephalopods.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9683700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of a new oxytrichid ciliate, Oxytricha buxai n. sp. and redescription of O. quadricirrata Blatterer and Foissner, 1988 based on morphology and 18S rDNA analyses 根据形态学和18S rDNA分析，对一种新的毛滴虫（Oxytricha buxai n.sp.）的描述以及对O.quadriirrata Blatterer和Foissner的重新描述，1988

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.1016/j.ejop.2023.125959

Daizy Bharti, Santosh Kumar

{"title":"Description of a new oxytrichid ciliate, Oxytricha buxai n. sp. and redescription of O. quadricirrata Blatterer and Foissner, 1988 based on morphology and 18S rDNA analyses","authors":"Daizy Bharti, Santosh Kumar","doi":"10.1016/j.ejop.2023.125959","DOIUrl":"10.1016/j.ejop.2023.125959","url":null,"abstract":"<div>The morphology of a new oxytrichid ciliate, Oxytricha buxai n. sp., isolated from a soil sample collected from the Buxa Tiger Reserve, West Bengal, India, was studied based on live observation and protargol impregnation. The new species is characterised by a body size of 85×35 µm in vivo, two macronuclear nodules with one or two micronuclei attached at variable positions, a few colourless cortical granules scattered throughout cortex, adoral zone of membranelles about 35% of body length with 26 membranelles on average, about 18 cirri in left and 16 cirri in right marginal row, right marginal row starts at the level of buccal vertex, usually 18 frontoventral transverse cirri, five dorsal kineties including one dorsomarginal row, three caudal cirri. Further, a redescription based on live and protargol-impregnated specimens of Oxytricha quadricirrata Blatterer and Foissner, 1988, isolated from a moss sample collected from the Kangra district, Himachal Pradesh, India, is provided. The Indian population of O. quadricirrata is similar in morphology to the type population. However, the dorsal side shows some variation, i.e., the presence of a second dorsomarginal row with one or two bristles and incomplete fragmentation of dorsal kinety 3 (vs single dorsomarginal row and complete fragmentation). The resting cyst is spherical and about 20 µm across, with a wrinkled surface. Morphogenesis is in typical Oxytricha pattern. Based on 18S rDNA, phylogenetic analyses show Oxytricha to be a polyphyletic genus. Further, O. quadricirrata clusters away from O. granulifera, thereby supporting the validity of the former.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toxicological impacts and likely protein targets of bisphenol a in Paramecium caudatum 双酚a对草履虫的毒理学影响及可能的蛋白质靶标

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-04-01 DOI: 10.1016/j.ejop.2023.125958

Marcus V.X. Senra , Ana Lúcia Fonseca

{"title":"Toxicological impacts and likely protein targets of bisphenol a in Paramecium caudatum","authors":"Marcus V.X. Senra , Ana Lúcia Fonseca","doi":"10.1016/j.ejop.2023.125958","DOIUrl":"10.1016/j.ejop.2023.125958","url":null,"abstract":"<div>Bisphenol A (BPA) is a widely used plasticizer agent and a well-known ubiquitous endocrine disruptor, which is frequently associated with a series of reproductive, developmental, and transgenerational effects over wildlife, livestocks, and humans. Although extensive toxicological data is available for metazoans, the impact of BPA over unicellular eukaryotes, which represents a considerable proportion of eukaryotic diversity, remains largely overlooked. Here, we used acute end-point toxicological assay and an inverted virtual-screening (IVS) approach to evaluate cellular impairments infringed by BPA over the cosmopolitan ciliated protist, Paramecium caudatum. Our data indicate a clear time-dependent effect over P. caudatum survival, which seems to be a consequence of disruptions to multiple core cellular functions, such as DNA and cell replication, transcription, translation and signaling pathways. Finally, the use of this ciliate as a biosensor to monitor BPA within environments and the relevance of bioinformatic methods to leverage our current knowledge on the impacts of emerging contaminants to biological systems are discussed.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9668536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0