Morphogenesis of the anaerobic ciliate Tropidoatractus levanderi (Ciliophora, Armophorea, Metopida), with notes on conjugation and molecular phylogeny

IF 1.6 2区生物学 Q4 MICROBIOLOGY

European journal of protistology Pub Date : 2025-09-15 DOI:10.1016/j.ejop.2025.126166

Xiaochen Feng , Wenbao Zhuang , Ran Li , Xiaozhong Hu

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Abstract

Tropidoatractidae, a recently established anaerobic family of metopid ciliates, have not yet been examined from a morphogenetic perspective. In this study, the interphase morphology, morphogenesis during binary fission, and body and certain nuclear changes during conjugation were investigated in a Chinese population of Tropidoatractus levanderi Rotterová et al., 2018 using the protargol impregnation method. The main findings are as follows: (1) the Chinese population closely resembles Czech and Brazilian populations, with the exception of a diplostichomonad type of paroral membrane, a feature previously overlooked; (2) divisional morphogenesis follows the metopid mode, providing further evidence for conserved cell division patterns within metopids, and (3) conjugation is of the total isogamontic type, a rare phenomenon among ciliates. Additionally, 18S rRNA gene sequence comparison and phylogenetic analyses were conducted to complement the morphological observations.

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厌氧纤毛虫troidatractus leanderi（纤毛虫目，纤毛虫目，Metopida目）的形态发生及其偶联和分子系统发育的注释

棘毛纤毛虫科是最近才建立的厌氧类纤毛虫科，但尚未从形态发生的角度进行研究。本研究采用protargol浸染法，对2018年中国鹿角蛙（Tropidoatractus levanderi rotterovi）居群的间期形态、二元裂变过程中的形态发生以及偶联过程中体和部分核的变化进行了研究。主要发现如下：(1)中国人群与捷克和巴西人群非常相似，除了口腔旁膜的diplostichomonad类型，这是一个以前被忽视的特征；(2)细胞的分裂形态发生遵循胞顶体模式，这进一步证明了胞顶体内细胞分裂模式的保守性；(3)偶联是完全等配型的，这在纤毛虫中是罕见的现象。此外，还进行了18S rRNA基因序列比较和系统发育分析，以补充形态学观察。

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来源期刊

European journal of protistology 生物-微生物学

CiteScore

4.60

自引率

20.70%

发文量

审稿时长

14.6 weeks

期刊介绍： Articles deal with protists, unicellular organisms encountered free-living in various habitats or as parasites or used in basic research or applications. The European Journal of Protistology covers topics such as the structure and systematics of protists, their development, ecology, molecular biology and physiology. Beside publishing original articles the journal offers a forum for announcing scientific meetings. Reviews of recently published books are included as well. With its diversity of topics, the European Journal of Protistology is an essential source of information for every active protistologist and for biologists of various fields.