European Journal of Integrative Medicine最新文献

{"title":"Pueraria lobata radix (Ge Gen)-containing traditional Chinese medicine prescriptions for the treatment of nonalcoholic fatty liver disease: A systematic review and meta-analysis","authors":"Haoyu Wang ,&nbsp;Huilin Liu ,&nbsp;Xiangmin Deng ,&nbsp;Ping Luo ,&nbsp;Yaqi Tian ,&nbsp;Rui Zhang ,&nbsp;Gaochuan Zhang ,&nbsp;Xunxun Yuan ,&nbsp;Xu Zhou","doi":"10.1016/j.eujim.2024.102405","DOIUrl":"10.1016/j.eujim.2024.102405","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to systematically evaluate the effectiveness and safety of <em>Pueraria lobata radix</em> (Chinese name: Ge Gen)-containing prescriptions (GGPs) for the treatment of nonalcoholic fatty liver disease (NAFLD).</div></div><div><h3>Methods</h3><div>Seven databases were searched for randomized controlled trials (RCTs) comparing the effectiveness of GGPs plus basic therapies (Western medicines or lifestyle interventions) versus the same basic therapies in patients with NAFLD. The primary outcomes were liver function indicators, and the secondary outcomes included metabolism indicators, severity of symptoms, and incidence of adverse events. The effects were measured as weighted mean differences (WMDs), standard mean differences (SMDs), and 95 % confidence intervals (CIs).</div></div><div><h3>Results</h3><div>Eighteen RCTs involving 1,991 patients were included. Meta-analysis results showed that compared to the control group, the GGP group had a significant improvement in liver function (aspartate aminotransferase: WMD -9.60 U/L, 95 % CI -14.96 to -4.25; alanine aminotransferase:6.30 U/L, 95 % CI -9.57 to -3.02; gamma-glutamyl transferase:6.35 U/L, 95 % CI -10.44 to -2.25), blood lipid levels (total cholesterol: WMD -0.68 mmol/L, 95 % CI -0.88 to -0.49; triglycerides:0.61 mmol/L, 95 % CI -0.84 to -0.38; low-density lipoprotein cholesterol:0.42 mmol/L, 95 % CI -0.56 to -0.27; high-density lipoprotein cholesterol: 0.34 mmol/L, 95 % CI 0.21 to 0.47), body mass index (WMD -0.79 kg/m², 95 % CI -1.38 to -0.19), blood glucose levels (fasting blood glucose: WMD -1.03 mmol/L, 95 % CI -1.52 to -0.44; 2-hour postprandial blood glucose:1.06 mmol/L, 95 % CI -1.68 to -0.28; glycosylated hemoglobin:0.56 %, 95 % CI -0.85 to -0.28; homeostatic model assessment of insulin resistance:0.85 mmol/L, 95 % CI -1.15 to -0.56; fasting insulin:2.34 mmol/L, 95 % CI -3.14 to -1.55), and symptom severity scores (SMD -1.91, 95 % CI -2.48 to -1.34). The GGP group reported six mild adverse events, including diarrhea, nausea, and stomach discomfort.</div></div><div><h3>Conclusion</h3><div>GGPs, as an adjunct therapy for NAFLD, may improve liver function, metabolic parameters, and clinical symptoms, with good safety. However, the quality of evidence was moderate-to-very low owing to the presence of a moderate-to-high risk of bias and significant heterogeneity.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102405"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142415979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DOTTs-Database of Therapeutic Plants against Tuberculosis: Rationalizing traditional medicine for therapeutic applications","authors":"Hazarika Risha ,&nbsp;Gangar Tarun ,&nbsp;Patra Sanjukta","doi":"10.1016/j.eujim.2024.102409","DOIUrl":"10.1016/j.eujim.2024.102409","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Mycobacterium tuberculosis,</em> a highly resistant superbug causing tuberculosis disease (TB), contributes to one of the leading causes of death worldwide. Improved drug regimens and therapeutics have been extensively studied, but still none are proven to be fully effective in eliminating the pathogen or its drug-resistant forms. Therefore, alternative and novel therapeutic approaches to address this problem are needed. Traditional medicine can be vital in managing TB via codified (Ayurveda, Siddha, Unani) and uncodified systems (folk medicine). In recent years, the utilization of medicinal plants for TB therapy has garnered considerable attention. Identifying underexplored plants utilized as a remedy for TB can aid in developing future treatment strategies. The current study aims to assemble the ethnobotanical literature that provides insight into the undocumented tribal/folk medicinal practices against tuberculosis in one unified platform.</div></div><div><h3>Methods</h3><div>The medicinal plants with therapeutic properties against tuberculosis were identified manually by screening published literature. The data is represented to the users using a user-friendly web interface developed via the PHP-HTML platform using Bootstrap Cascading Style Sheet (CSS) theme, and the database is managed by MySQL Database Management System (DBMS).</div></div><div><h3>Results</h3><div>Database of Therapeutic Plants against Tuberculosis (DOTTs) describes the medicinal plants used traditionally in different forms against tuberculosis by various indigenous tribes and communities in India. A total of 100 plants with their local names, taxonomical classification (order and family), tribe, plant part, and formulations, as well as the anti-tubercular attributes, are provided in the database. Additional information about the plants can be retrieved from the external links provided.</div></div><div><h3>Conclusion</h3><div>The DOTTs database provides the ethnobotanical and ethnopharmacological data of 100 Indian medicinal plants. This database will help the researchers acquire information about the plants that could be beneficial for further evaluation and discovery of potential leads in TB drug discovery. With proper investigation and advancement, it is anticipated that TB could no longer be a serious public health risk.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102409"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of music interventions on anxiety, pain, and pregnancy rates in women undergoing fertility treatment before oocyte collection and embryo transfer: Systematic review and meta-analysis of randomized controlled trials","authors":"Mehtap Kızılkaya ,&nbsp;Aysu Yıldız Karaahmet ,&nbsp;Kerime Derya Beydağ","doi":"10.1016/j.eujim.2024.102408","DOIUrl":"10.1016/j.eujim.2024.102408","url":null,"abstract":"<div><h3>Introdution</h3><div>The present review aimed to evaluate the available evidence in the literature on the effect of music interventions on improving outcomes related to anxiety, pain, and pregnancy rates of women undergoing fertility treatment, prior to oocyte collection and embryo transfer.</div></div><div><h3>Method</h3><div>We searched the following databases from database inception to 2 May 2024, PUBMED, Embase, Web of Science, and the Turkish Academic Network and Information Center (ULAKBIM). Randomized controlled trials (RCT) in Turkish and English languages were included in the screening. Articles were scanned using MeSH-based keywords. The data were analyzed using the Review Manager computer program (Version 5.4.1).</div></div><div><h3>Results</h3><div>A total of 8 RCTs, including 1634 female patients, were included in our review. Music interventions significantly reduced anxiety scores prior to oocyte collection or fertilization compared to the control group (Standardised Mean Difference (SMD):0.26, 95 % CI:0.39 to 0.13, <em>Z</em> = 3.91, <em>p</em> &lt; 0.0001). Moreover, after the music treatment, there was a significant improvement in the pain score during the procedure (SMD:0.96, 95 % CI:1.72 to -0.20, <em>Z</em> = 2.49, <em>p</em> = 0.01). Although more women in the music interventions group experienced an increase in the clinical pregnancy rate compared to the control group, there was no evidence of an effect on pregnancy rate (SMD:0.14, 95 % CI:2.63 to 2.34, <em>Z</em> = 0.11, <em>p</em> = 0.91).</div></div><div><h3>Conclusion</h3><div>Music interventions appears to improve anxiety and pain scores in female patients receiving fertility treatment when administered during oocyte collection and fertilization. They may increase the clinical pregnancy rate but this not statistically significant. More research with larger sample sizes is needed to investigate the impact of music interventions on assisted reproductive technology clinical outcomes.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"72 ","pages":"Article 102408"},"PeriodicalIF":1.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yiqi Huoxue Tongluo formula-containing serum attenuates high glucose-induced injury in microglia via MAPK pathways: Network pharmacology-based analysis and biological validation","authors":"Chang Chang ,&nbsp;Qian Chen ,&nbsp;Ke Zuo ,&nbsp;Huilun Yuan ,&nbsp;Lujie Wang ,&nbsp;Aijuan Jiang ,&nbsp;Yuqing Wang","doi":"10.1016/j.eujim.2024.102403","DOIUrl":"10.1016/j.eujim.2024.102403","url":null,"abstract":"<div><h3>Introduction</h3><div>The Yiqi Huoxue Tongluo formula (YQHX), containing Astragalus mongholicus Bunge, Angelica sinensis (Oliv.) Diels, Rehmannia glutinosa Libosch., Pueraria lobata (Willd.) Ohwi, Corydalis yanhusuo W.T.Wang, Spatholobus suberectus Dunn, Clematis chinensis Osbeck, is a proprietary formula of the First Affiliated Hospital of Anhui University of Chinese Medicine, and can attenuate diabetic neuropathy pain (DNP). This study aimed to explore the mechanisms underlying YQHX-mediated DNP alleviation. Additionally, the effects of YQHX on BV2 microglia injured by high glucose via the mitogen-activated protein kinase (MAPK) signaling pathways were investigated.</div></div><div><h3>Methods</h3><div>Liquid chromatography tandem mass spectroscopy (LC-MS/MS) was performed to identify the chemical compounds in YQHX. The potential mechanism of YQHX for treating DNP was explored through network pharmacology analysis. The established glucose-induced BV2 cell injury model was treated with varying concentrations of YQHX-containing serum. The effects of YQHX-containing serum and MAPK pathway inhibitors on microglial cells were assessed by Cell Counting Kit-8 and enzyme-linked immunosorbent assays. The messenger RNA (mRNA) and protein levels of microglial marker OX42 were measured. Additionally, phosphorylated-p38, -c-Jun-terminal kinase, and -extracellular signal-regulated kinase and downstream nuclear factor-κB/p65 and phosphorylated-activating transcription factor 2 (ATF2) proteins were detected by western blotting.</div></div><div><h3>Results</h3><div>In total, 503 chemical components were identified in YQHX by LC-MS/MS analysis. Network pharmacology results showed 203 active ingredients and 363 overlapping targets, respectively, as potential therapeutic candidates for YQHX-mediated DNP treatment. Additionally, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis yielded a total of 105 signal pathways. Notably, YQHX considerably improved the cell viability of BV2 cells (<em>P</em> &lt; 0.01), remarkably reduced the production of proinflammatory factors (<em>P</em> &lt; 0.01), significantly increased the production of anti-inflammatory factors (<em>P</em> &lt; 0.01), and markedly inhibited the mRNA and protein levels of OX42 (<em>P</em> &lt; 0.01). Furthermore, YQHX treatment inhibited MAPK activation and ATF2 expression.</div></div><div><h3>Conclusions</h3><div>YQHX may protect against high-glucose-induced BV2 microglial cell injury by inhibiting the activation of MAPKs and downstream ATF2, thus, reducing microglial activation and proinflammatory cytokine release. These results provide a foundational theoretical basis for exploring the further mechanism of YQHX in treating DNP, and they provide a novel target for the application of traditional Chinese medicine.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102403"},"PeriodicalIF":1.9,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Adverse events associated with the use of cannabis-based products in people living with cancer: A scoping review protocol” [European Journal of Integrative Medicine, Volume 62, 102279, 2023]","authors":"Irene Cheah ,&nbsp;Ingrid Gelissen ,&nbsp;Jennifer Hunter ,&nbsp;Joanna Harnett","doi":"10.1016/j.eujim.2024.102402","DOIUrl":"10.1016/j.eujim.2024.102402","url":null,"abstract":"","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102402"},"PeriodicalIF":1.9,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1876382024000714/pdfft?md5=39daadce02743dfdf35a63b3b956547d&pid=1-s2.0-S1876382024000714-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of medicinal herbs among soccer athletes in the West Bank of Palestine","authors":"Faten Amer ,&nbsp;Mahmoud Alatrash","doi":"10.1016/j.eujim.2024.102398","DOIUrl":"10.1016/j.eujim.2024.102398","url":null,"abstract":"<div><h3>Introduction</h3><p>Herbal supplements are popular among athletes, yet research on their types, purposes, and information sources is limited, particularly for Palestinian soccer players. This study aims to fill this gap by examining the types of herbal supplements used by young soccer athletes in the West Bank, their purposes, and the sources of information influencing their use. Understanding these aspects is crucial for safely and effectively integrating traditional remedies into modern sports nutrition.</p></div><div><h3>Methods</h3><p>In 2023, we conducted a cross-sectional study with young soccer athletes in the West Bank. We utilized a validated survey to collect data on the types of herbal supplements, their preparation methods, and information sources. We analyzed the data for frequencies and use values (UV).</p></div><div><h3>Results</h3><p>Out of 165 invited athletes, 155 participated, with 144 confirming their current use of herbal supplements. The participants, aged 18–25 (mean age 20.34), employed a variety of preparation and consumption methods. Information sources comprised traditional knowledge, advice from herbalists (\"Attar\" in Arabic), and online trends. Herbs that enhance physical endurance and stamina had the highest UV of 3.93, highlighting their critical importance. Herbs for joint and muscle pain and central nervous system stimulation had UV of 2.28 and 2.10, respectively, reflecting their significant roles. Antistress and anabolic herbs had UVs of 1.28 and 1.21, respectively. The total UV of 10.81 indicates a substantial use of herbal supplements. We recorded 1,675 recommendations, with the primary uses being: enhancing physical endurance and strength (36.36 %), reducing joint and muscle pain (21.13 %), acting as a CNS stimulant (19.46 %), alleviating stress (11.82 %), and providing testosterone-like effects (11.22 %). The top 10 herbs were ginseng (7.46 %), small nettle (6.99 %), olive (6.63 %), date (5.85 %), chili pepper (5.43 %), green tea (5.19 %), fenugreek (4.84 %), soy (4.24 %), coffee (4.24 %), and mate (4.12 %), collectively accounting for about 55 % of all recommendations.</p></div><div><h3>Conclusion</h3><p>This study identifies the top 10 herbs used by soccer athletes, as well as their role in modern sports nutrition. Future research should evaluate their effectiveness and safety, focusing on herbs like date and small nettle. Educating athletes on the proper use and risks of herbal supplements is essential.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"70 ","pages":"Article 102398"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of adlay seed extract on the level of neuroprotection gene expression in human nasal orbital mesenchymal stem cells","authors":"Fatemeh Sanie-Jahromi,&nbsp;Ali Azimi,&nbsp;Hamidreza Hassanipour,&nbsp;Pardis Bostanian,&nbsp;Behzad Khademi","doi":"10.1016/j.eujim.2024.102397","DOIUrl":"10.1016/j.eujim.2024.102397","url":null,"abstract":"<div><h3>Introduction</h3><p>Stem cells have gained attention for their potential as a promising approach for generating neurotrophins and advancing cell-based therapies for retinal degenerative diseases such as glaucoma. This study aimed to explore how adlay seed extract impacts the gene expression of key components within the neuroprotection pathway (<em>NGF, TRKB, MAPK, PI3K</em>) in human nasal orbital adipocytes mesenchymal stem cells (OAMSCs).</p></div><div><h3>Methods</h3><p>Nasal OAMSCs, with a density of 10⁶ cells/ 10 cm², were subjected to a 24-hour exposure to adlay seed extracts (namely the methanolic (MeOH) and residual (Res) fractions at a concentration of 1 mg/ml). The control group received an identical medium without the extract at the same time and under the same circumstances. We measured the relative expression levels of nerve growth factor <em>(NGF)</em>, tyrosine receptor kinase B <em>(TRKB)</em>, mitogen-activated protein kinase <em>(MAPK)</em>, and phosphoinositide 3-kinase <em>(PI3K)</em> in the treated cells versus the control group using real-time polymerase chain reaction (PCR).</p></div><div><h3>Results</h3><p>Our data revealed that both the MeOH and Res extracts of adlay seed led to a significant upregulation of <em>NGF</em> in nasal OAMSCs. MeOH extract also led to the overexpression of <em>TRKB</em> (the gene coding for BDNF receptor) in OAMSCs, while the other genes understudy, were not altered.</p></div><div><h3>Conclusions</h3><p>Our study highlighted initial documentation of the stimulating impact of adlay extract on the transcriptional level of neurotrophin <em>NGF</em> and the <em>TRKB</em> in nasal OAMSCs. We also showed that the extraction method could significantly affect the molecular-level properties of adlay. This preliminary study can pave the ground for future advancements in the treatment of retinal neurodegenerative disorders and glaucoma.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"70 ","pages":"Article 102397"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and effect of a new artificial saliva containing Zingiber officinale and Cuminum cyminum on stimulating saliva secretion: In vitro, in vivo and preliminary clinical study","authors":"Supamas Napavichayanun ,&nbsp;Sutima Luangdilok ,&nbsp;Rungnapha Yamdech ,&nbsp;Pornanong Aramwit","doi":"10.1016/j.eujim.2024.102396","DOIUrl":"10.1016/j.eujim.2024.102396","url":null,"abstract":"<div><h3>Introduction</h3><p>Xerostomia is a symptom that affects quality of life and may lead to malnutrition. While artificial saliva is used as a lubricant for the oral cavity, it rarely stimulates saliva secretion. The objective of this study was to develop a herbal artificial saliva (HAS) that could stimulate saliva secretion and be safe for human consumption.</p></div><div><h3>Methods</h3><p>Three HAS formulations were prepared with 0.3 % <em>Cuminum cyminum</em> extract and 0.05 %, 0.10 %, and 0.15 % <em>Zingiber officinale</em> extract (HAS0.05, HAS 0.10, and HAS0.15, respectively). <em>In vivo</em> skin and oral mucosal irritation tests were used to evaluate the safety of HAS0.15. Additionally, the preliminary efficacy and safety of the three HAS formulations were investigated in 21 healthy volunteers. The sample was sprayed into the oral cavity under the left and right sides of the tongue. Thirty minutes after spraying, the amount of saliva was assessed using a visual analogue scale and a Schirmer strip. In addition, dry mouth symptoms, the saliva pH, and adverse reactions were assessed before and after testing.</p></div><div><h3>Results</h3><p>HAS0.15 caused minimal skin irritation and did not alter the epithelium or other structures of the oral mucosa. The saliva quantity after using HAS0.10 and HAS0.15 was significantly higher than prior to testing (<em>p</em> &lt; 0.05). None of the formulations induced mucositis or adverse reactions.</p></div><div><h3>Conclusion</h3><p>This study was successful in the development of a new HAS that stimulated saliva secretion and was safe for humans. However, this study is preliminary: Further clinical investigation in patients with hyposalivation will confirm the efficacy of this novel HAS. This novel HAS may be an alternative treatment for the elderly and patients with hyposalivation.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"70 ","pages":"Article 102396"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parkinsonia praecox bark as a new source of antibacterial and anticancer compounds","authors":"Armando Ordaz-Hernández ,&nbsp;Beatriz Hernández-Carlos ,&nbsp;Héctor Manuel Arreaga González ,&nbsp;Lorena Hernández-Ramiro ,&nbsp;Misael Corona Ramírez ,&nbsp;Mayra Herrera-Martínez","doi":"10.1016/j.eujim.2024.102401","DOIUrl":"10.1016/j.eujim.2024.102401","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Parkinsonia praecox</em> is used in traditional medicine to treat various diseases; however, the antibacterial and anticancer properties from its bark have not been reported. Thus, this study aimed to evaluate the antibacterial activity, cytotoxicity and chemical study of methanolic extract of <em>Parkinsonia praecox</em> bark (PpBM).</p></div><div><h3>Methods</h3><p>The PpBM antibacterial activity was determined against pathogen strains using the broth microdilution method, the cytotoxic effect was assessed on cancerous (HepG2, SKOV-3 and HeLa) and non-cancerous (J774A.1 and HaCaT) cells using the crystal violet assay, the <em>in vitro</em> hemotoxicity was evaluated using the hemolysis assay on human erythrocytes and the identification of major compounds derived from PpBM was performed by ¹H and ¹³C NMR and 2D NMR experiments.</p></div><div><h3>Results</h3><p>PpBM (2000 µg/mL) demonstrated antibacterial activity against <em>Listeria monocytogenes</em>, a potential breakthrough in the fight against this pathogen. PpBM decreased cell viability of HepG2 liver cancer cells (IC₅₀, 104.56 µg/mL) with a selectivity index of 1.38 regarding J774A.1 macrophages and 2.04 to HaCaT keratinocytes, suggesting a selective anticancer potential. On the other hand, PpBM did not cause hemolysis to high concentrations (1000 µg/mL) or alterations in the morphology of human erythrocytes at doses lower than 200 µg/mL. For the first time, lupenone, germanicone, 3-oxo-oleanane-18-en-28-ol, and combretol were identified in <em>P. praecox</em>. PpBM showed antibacterial, selective anticancer, and non-hemotoxic properties. These activities may be related to the major compounds, such as lupenone.</p></div><div><h3>Conclusion</h3><p><em>P. praecox</em> bark is a new option for obtaining bioactive compounds, further <em>in vitro</em> and <em>in vivo</em> studies are required to confirm their efficacy and safety.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102401"},"PeriodicalIF":1.9,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the anti-inflammatory mechanism and effective constituents of Hosta plantaginea flowers by a strategy integrating network pharmacology, molecular docking, and experimental verification","authors":"Bowei Xia ,&nbsp;Xiaomei Li ,&nbsp;Qingcui Zhang ,&nbsp;Zhongwei He ,&nbsp;Jian Liang ,&nbsp;Junwei He ,&nbsp;Wanchun Wang ,&nbsp;Degang Dong ,&nbsp;Li Yang","doi":"10.1016/j.eujim.2024.102400","DOIUrl":"10.1016/j.eujim.2024.102400","url":null,"abstract":"<div><h3>Introduction</h3><div>Inflammation is a common cause of illness among outpatients, but effective drugs are extremely scarce. <em>Hosta plantaginea</em> flower is an important herbal medicine in China that treats inflammation-related diseases, but its anti-inflammatory mechanism remains unclear.</div></div><div><h3>Methods</h3><div>Systematic network pharmacology was employed to decipher <em>H. plantaginea</em> flower's anti-inflammatory hub targets and candidate phytochemicals. Furthermore, molecular docking was used to elucidate the interaction of constituents and targets. Afterward, the anti-inflammatory activity of <em>H. plantaginea</em> flower and its mechanism were validated in RAW264.7 cells activated by lipopolysaccharide (LPS).</div></div><div><h3>Results</h3><div><em>H. plantaginea</em> flower contains 153 phytochemicals, of which 85 were potential effective constituents. 949 <em>H. plantaginea</em> flower targets and 814 inflammation-related targets were predicted, of which 211 intersecting targets were acquired. Six major constituents, including corvitin, phytyldiol, agapanthagenin, 5,7-dimethoxy-8-methyl-4′-hydroxyflavan, (3<em>R</em>)-2,3-dihydro-7-hydroxy-3-[(4-methoxyphenyl)methyl]-4H-1-benzopyran-4-one, and phenol, 4-[(2<em>S</em>)-3,4-dihydro-5,7-dimethoxy-2H-1-benzopyran-2-yl] were picked out. Furthermore, 6 hub targets such as C-type lectin-like receptor 2 (CLEC1B), toll-like receptor (TLR), tumor necrosis factor (TNF), recombinant V-Rel reticuloendotheliosis viral oncogene homolog A (RELA), signal transducer and activator of transcription 3 (STAT3), and protein kinase B α (AKT1) were obtained using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional analyses. Molecular docking indicated that these 6 constituents had strong binding abilities with these 6 hub targets. Finally, the anti-inflammatory effect of <em>H. plantaginea</em> flower and its mechanism were verified in LPS-induced RAW264.7 cells by the inhibition of CLEC1B, TLR-4, TNF-α, STAT3, and Akt1 proteins, thereby suppressing the overproduction of nitric oxide.</div></div><div><h3>Conclusion</h3><div><em>H. plantaginea</em> flower exerted a significant anti-inflammatory activity by suppressing TLR-4 and CLEC1B signaling pathways, which was elucidated and validated by an integrated strategy based on network pharmacology, molecular docking, and experimental verification. Collectively, this study provides a novel insight into the pharmacodynamics and mechanisms of <em>H. plantaginea</em> flower as a promising agent in treating inflammation-related disease.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102400"},"PeriodicalIF":1.9,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}