European Journal of Integrative Medicine最新文献

{"title":"Exploring the potential of Cytisus purgans as a source of bioactive molecules: In vitro pharmacological evaluation","authors":"Safa Chaira ,&nbsp;Badra Bouzghaia ,&nbsp;Mourad Hanfer ,&nbsp;Imededdine Kaddi ,&nbsp;Mohammed Tahar Ben Moussa ,&nbsp;Patrick Pale ,&nbsp;Hassina Harkat","doi":"10.1016/j.eujim.2024.102349","DOIUrl":"10.1016/j.eujim.2024.102349","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Cytisus purgans</em> is an uncommon species found in Algeria (Aures, Mahdids and Lella Khadidja). In the current study, we present, to the best of our knowledge, the first detailed chemical profile of its extracts and collect various pieces of evidence that underscore their therapeutic potential.</p></div><div><h3>Methods</h3><p>The ethyl acetate extract (EAE) of <em>C. purgans</em> was purified using different chromatographic methods. The bioactive compounds obtained were analysed by NMR and mass spectroscopy to identify their structures. The therapeutic potential of the extracts was investigated using several in vitro assays.</p></div><div><h3>Results and discussion</h3><p>The developed spectral analysis techniques enabled the structural elucidation of seven compounds. The analysis identified four isoflavones, including daidzein, genistein, isoprunetin and biochanin A. Additionally, one flavone, chrysin and one flavonol, quercetin, were positively identified. Notably, the phytosterol daucosterol was also isolated and characterised. The studied extracts were found to be rich in polyphenols and flavonoids. The EAE had the highest polyphenolic (163.85 ± 3.82 mg GAE/g dry extract) and flavonoid content (180.84 ± 3.68 mg QE/g dry extract). The extracts showed potent anti-oxidant activity, as demonstrated by DPPH 50 % inhibitory concentration (EAE: IC₅₀ = 26.66 µg/mL, BuE: IC₅₀ = 30.91 µg/mL), ferric reducing anti-oxidant power IC₅₀ (EAE: IC₅₀ = 6.37 ± 0.27 µg/mL, BuE: IC₅₀ = 13.02 ± 0.1 µg/mL) and total anti-oxidant capacity values (EAE 106.57 ± 5.81 AAE/g dry extract, BuE 105.46 ± 8.64 AAE/g dry extract). This anti-oxidant activity contributed significantly to effective sun protection (EAE sun protection factor (SPF) = 87.84, BuE SPF = 31.67). Additionally, the extracts inhibited acetylcholinesterase (EAE IC₅₀ = 24.40 ± 2.84 µg/mL, BuE IC₅₀ = 68.07 ± 02.26 µg/mL) and butyrylcholinesterase enzymes (EAE IC₅₀ = 36.39 ± 0.75 µg/mL, BuE IC₅₀ = 66.19 ± 01.25 µg/mL) the most and significantly inhibited urease (EAE IC₅₀ = 77.82 ± 4.59 µg/mL, BuE IC₅₀ = 127.05 ± 2.87 µg/mL).</p></div><div><h3>Conclusion</h3><p>Considering the findings, <em>C. purgans</em> emerges as a promising reservoir of bioactive compounds that have the potential to contribute to the development of treatments for specific diseases. The identification of these compounds and their therapeutic potential provides valuable insights into their potential use in drug development.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"67 ","pages":"Article 102349"},"PeriodicalIF":2.5,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139954041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating yoga into comprehensive cancer care: Starting somewhere","authors":"Kathryn M. Glaser ,&nbsp;Christina R. Crabtree-Ide ,&nbsp;Tessa F. Flores ,&nbsp;Mary E. Reid","doi":"10.1016/j.eujim.2024.102348","DOIUrl":"10.1016/j.eujim.2024.102348","url":null,"abstract":"<div><h3>Introduction</h3><p>The rapid increase in cancer survivors in the United States (US) highlights the importance of survivors’ long-term care needs and symptom management. Given evidence that yoga supports cancer related symptoms, our aim was to develop and implement a community yoga program available for cancer survivors, family members, and staff involved in cancer care at a public comprehensive cancer center in the US. Given the wide age range and potential side effects from cancer treatment, we adapted the yoga program to meet the needs of cancer survivors.</p></div><div><h3>Methods</h3><p>Beginning in May 2017, we offered sixty-minute gentle yoga sessions for all medically cleared cancer survivors and their family members, caregivers, and friends, as well as oncology staff. We aimed to evaluate acceptability, development, and implementation of an adaptive yoga program at our comprehensive cancer center using mixed methods. Classes were initially offered once a week, then increased to twice a week. A total of five service evaluations were sent approximately every six months between April 2018-October 2019 to evaluate participant satisfaction (<em>n</em> = 90 responses). Qualitative data were collected and analyzed using thematic analysis of participant satisfaction, allowing us to continuously monitor the yoga program and adjust to meet participants’ needs.</p></div><div><h3>Results</h3><p>Between May 2017 and February 2020, 176 people attended our program. Ages ranged from 21 to 72 years, with an average age of 45 years. Based on these evaluations, 96 % (<em>n</em> = 86) of participants rated both the program and teachers excellent, and 4 % (<em>n</em> = 4) rated the program and teachers good. Qualitative themes highlighted positive aspects of the program, including accessibility, inclusivity, and adaptive yoga based on medical needs, skill level, and abilities.</p></div><div><h3>Conclusions</h3><p>Adaptive yoga programs are well-accepted by cancer survivors and caregivers, and may contribute a solution to healthcare staff and physician burnout. It is essential to build a solid foundation of self-care and wellness programming for cancer survivors and to create and foster adaptive yoga classes. Next steps for our program include evaluating yoga within the clinical setting, including chemotherapy infusion centers, and further research on reduction in stress through yoga in cancer survivors.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"67 ","pages":"Article 102348"},"PeriodicalIF":2.5,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139954064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The add-on effect of oral East Asian herbal medicine to steroid therapy for alopecia areata: A systematic review and meta-analysis","authors":"Soojin Park ,&nbsp;Jieun Lee ,&nbsp;Seoyeon Jun ,&nbsp;Kyuseok Kim","doi":"10.1016/j.eujim.2024.102345","DOIUrl":"10.1016/j.eujim.2024.102345","url":null,"abstract":"<div><h3>Introduction</h3><p>Alopecia areata (AA) is an autoimmune disease that degrades patients' quality of life and adversely affects mental health. Since steroid therapy (ST) has limited efficacy and a risk of recurrence, East Asian herbal medicine (EAHM) combined with ST has been suggested as an alternative treatment. Many randomized controlled trials (RCTs) have been conducted on the efficacy of EAHM combined with ST in AA treatment; however, the efficacy of EAHM combined with ST and the treatment effects of ST alone have not yet been compared. This systematic review and meta-analysis aimed to evaluate the effectiveness and safety of the combination of ST and EAHM compared to ST alone in the treatment of AA.</p></div><div><h3>Methods</h3><p>We searched 10 databases: PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang, KoreaMed, Korean Medical Database (KMBASE), Korean Studies Information Service System (KISS), ScienceON, and Korea Science. Bias was independently assessed using Cochrane Risk of Bias tool (RoB 2.0), and meta-analysis was conducted using Review Manager (Revman) 5.4.1 with the application of random-effect models. The primary outcome was defined as the cure rate.</p></div><div><h3>Results</h3><p>Fifteen studies were selected, and 1,119 participants were included in the study. According to the results of the meta-analysis, a higher cure rate (RR 1.50, 95 % CI : 1.31, 1.71, <em>P</em>&lt;0.00001, I²=0 %), a higher total efficacy rate (modified Total Efficacy Rate 1 (mTER1); (RR 1.10, 95 % CI: 1,06, 1.15, <em>P</em> &lt; 0.00001, I²=0 %), modified Total Efficacy Rate 2 (mTER2); (RR 1.09, 95 % CI: 1.03, 1.16, <em>P</em> = 0.006, I²=0 %)), and a lower recurrence rate (RR 0.28, 95 % CI: 0.16, 0.48, <em>P</em>&lt;0.00001, I²=0 % %) were confirmed in the ST with EAHM group than in the ST group. Low IFN-γ levels and high IL-10 levels were identified in the ST combined with EAHM group. No serious adverse effects were observed during treatment.</p></div><div><h3>Conclusions</h3><p>Compared to ST alone in AA treatment, the combination treatment of ST and EAHM may effectively and safely increase the cure and total efficacy rates and may lower the recurrence rate. However, to strengthen the basis of this review, higher quality RCTs must be performed.</p></div><div><h3>Registration</h3><p>CRD42023397023</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"68 ","pages":"Article 102345"},"PeriodicalIF":2.5,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139920521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of catgut embedding in patients with obesity of gastrointestinal internal heat: A randomised controlled trial","authors":"Qingqing Zhu ,&nbsp;Jian Cheng ,&nbsp;Xiaolan Xu ,&nbsp;Tangying Xu ,&nbsp;Xingjiang Gu ,&nbsp;Yun Xia ,&nbsp;Lingzhen Mao ,&nbsp;Nannan Hu ,&nbsp;Yuexia Fang","doi":"10.1016/j.eujim.2023.102327","DOIUrl":"10.1016/j.eujim.2023.102327","url":null,"abstract":"<div><h3>Introduction</h3><p>Globally, the incidence of obesity is increasing, causing increased rates of hypertension, diabetes, and cardiovascular diseases. Acupoint catgut embedding, a popular acupuncture method in China, has greater efficacy than acupuncture for weight control. The effectiveness of catgut embedding in patients with fatty liver associated with metabolic dysfunction was investigated.</p></div><div><h3>Methods</h3><p>In this randomised controlled trial, 60 patients with gastrointestinal internal heat-type obesity were randomised to the intervention (<em>n</em> = 30) and control (<em>n</em> = 30) groups. The intervention group participated in the catgut embeddings with diet and exercise for 2 months, with a catgut treatment frequency of once every 10 days for 30 min. The control group participated only in diet and exercise for the same period. Body weight; body mass index (BMI); waist-to-hip ratio (WHR); fasting plasma glucose, 2-hour blood glucose, glycated haemoglobin, and lipid levels were assessed before and after treatments.</p></div><div><h3>Results</h3><p>Fifty-seven patients completed the courses. After 2 months, the intervention group showed a statistically significant trend of decrease in WHR and total score of gastrointestinal internal heat-type clinical symptoms when compared with the control group (mean difference: −0.03, 95 % confidence level [CI]: −0.07 to 0.00 and mean difference: −6.04, 95 % CI: −8.59 to −3.50, respectively). In the intervention group, the mean reductions in body weight and BMI were 6.18 kg (95 % CI: 4.60 to 7.76) and 2.23 kg/m² (95 % CI: 1.72 to 2.74), respectively. The total treatment efficiency for fatty liver in the intervention and control groups was 61.5 % and 30.8 %, respectively (risk ratio: 3.60; 95 % CI: 1.14 to 11.35).</p></div><div><h3>Conclusion</h3><p>Catgut embedding administered with diet and exercise is effective for patients with gastrointestinal internal heat-type obesity and some patients with obesity complicated by metabolic dysfunction-associated fatty liver disease. The effects observed in the intervention group were superior to those in the control group in terms of body weight, BMI, WHR, total score of gastrointestinal internal heat-type clinical symptoms, and treatment effectiveness for metabolic dysfunction-associated fatty liver disease. There were no serious adverse reactions during the treatment.</p></div><div><h3>Registration number</h3><p>ChiCTR2200057237.</p></div><div><h3>Funding</h3><p>This study was supported by the Hangzhou Medicine and Health Science and Technology Program and Hangzhou Lin'an District Hospital of Traditional Chinese Medicine.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102327"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1876382023001038/pdfft?md5=1e0505cc3272ba77d025c5185e34708c&pid=1-s2.0-S1876382023001038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138683913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Korean medicine therapies in children with allergic rhinitis: A prospective, multicenter, observational registry study at primary-care network clinics","authors":"Hongmin Chu ,&nbsp;EunKyoung Lee ,&nbsp;Seunghwan Moon PharmB ,&nbsp;Hamsoa Clinics KM Doctors Group ,&nbsp;Bo-Hyoung Jang","doi":"10.1016/j.eujim.2024.102336","DOIUrl":"10.1016/j.eujim.2024.102336","url":null,"abstract":"<div><h3>Introduction</h3><p>Allergic rhinitis (AR) causes symptoms such as hyper-rhinorrhea, sneezing, nasal congestion, itching, and pain by triggering inflammation in the nasal mucosa and an immune response caused by antigen-specific Immunoglobulin E (IgE). This prospective multicenter observational study aimed to evaluate the symptoms change and safety of combined Korean medicine (KM) therapy in patients with AR.</p></div><div><h3>Methods</h3><p>This study was conducted in HAMSOA Korean Medicine Clinics, a network specializing in treating pediatric patients in Korea, and 15 centers participated. The patients were divided into three groups (mild, moderate, and severe) according to their baseline total nasal symptom score. The patients were treated with herbal medicine, acupuncture, cupping therapy, and moxibustion therapy.</p></div><div><h3>Results</h3><p>The study involved 72 subjects aged 6.47 ± 2.37 years. The average Numeric Rating Scale for allergic rhinitis symptoms at the baseline and final follow-up were 4.39 ± 2.14 to 3.47 ± 2.51, respectively, indicating a significant improvement over an average of 4 weeks. The Pediatric Allergic Disease Quality of Life Questionnaire scores at the final screening improved in all three groups. The mean difference was -10.44, with a 95 % confidence interval of -15.35 to -5.54 (<em>p</em> &lt; 0.01.). Furthermore, no adverse events were reported in any patient in this study.</p></div><div><h3>Conclusion</h3><p>The results provide preliminary evidence suggesting that KM treatment may be a safe and effective option for reducing symptoms associated with allergic rhinitis in children. Additional prospective studies are required to confirm the results of the present study.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102336"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139510331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting indicators of acupuncture service quality using analytic hierarchy process","authors":"Chao-Chan Wu ,&nbsp;Chiung-Chen Ho ,&nbsp;Kai-Chun Yang","doi":"10.1016/j.eujim.2023.102324","DOIUrl":"10.1016/j.eujim.2023.102324","url":null,"abstract":"<div><h3>Introduction</h3><p>In traditional Chinese medicine (TCM) services, formulating management strategies and policies to improve service quality is essential for maintaining competitive advantages. The purpose of this study is to select key indicators of acupuncture service quality to assist in the development of relevant strategies in order to guide service management in TCM clinics.</p></div><div><h3>Methods</h3><p>This study uses analytic hierarchy process (AHP) to identify key indicators of acupuncture service quality. A hierarchical architecture comprising four main criteria and twelve sub-criteria was established based on a solid theoretical foundation. Next, the judgements of experts in pairwise comparisons were used to calculate the weight of each criterion and determine the critical criteria for acupuncture service quality. Furthermore, fuzzy AHP was utilized to validate the effectiveness of AHP.</p></div><div><h3>Results</h3><p>Of all the main criteria, “outcome quality” was the most important, followed by “interaction quality”. Among the twelve sub-criteria, “patient satisfaction” was deemed as the most important, with “expertise” and “waiting time” ranking second and third, respectively. “Interactional justice” and “process quality” were ranked fourth and fifth, respectively.</p></div><div><h3>Conclusions</h3><p>The key findings indicate outcome quality, interaction quality, and the five sub-criteria are key indicators of acupuncture service quality. Therefore, time-saving strategies for operating procedures and human resource policies should be developed to improve acupuncture services.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102324"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138683914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of vitamin D supplementation in pregnancy on the incidence of preeclampsia: A systematic review and meta-analysis","authors":"Zainab Alimoradi ,&nbsp;Farideh Kazemi ,&nbsp;Azita Tiznobeik ,&nbsp;Mark D. Griffiths ,&nbsp;Seyyedeh Zahra Masoumi ,&nbsp;Soodabeh Aghababaei","doi":"10.1016/j.eujim.2024.102343","DOIUrl":"10.1016/j.eujim.2024.102343","url":null,"abstract":"<div><h3>Introduction</h3><p>The results of previous studies regarding the effect of vitamin D on the incidence of preeclampsia are inconsistent. Therefore, the primary objective of the present review was to determine the effect of vitamin D supplementation during pregnancy on the risk of preeclampsia.</p></div><div><h3>Methods</h3><p>Five major scientific databases were searched from inception to June 10, 2023. Studies with randomized controlled trial designs were identified. To assess the methodological quality of the selected studies, the Cochrane Tool Checklist (CTC) was used. The random effect model was chosen as a combination model. Statistical heterogeneity was evaluated using the standard χ² test, and the intensity of heterogeneity was calculated using <em>I</em>². Effect size indicators including risk ratio (RR), risk difference (RD), and number needed to treat (NNT) were calculated with estimated 95 % confidence intervals.</p></div><div><h3>Results</h3><p>Nineteen studies were included in the systematic review and meta-analysis. The pooled RR of preeclampsia in the intervention group compared to the control group was 0.61 (95 % CI, 0.47 to 0.78; <em>I²</em>=14.4 %; χ²=23.37; <em>p</em> = 0.27; tau²=0.05), and indicated a 39 % reduction in the risk of preeclampsia. The pooled RD of preeclampsia in the intervention group compared to the control group was -0.03 (95 % CI: -0.05 to -0.01; <em>I²</em>=45.5 %; χ²=36.68; <em>p</em> = 0.01; tau²=0.0008) and the difference in the risk of preeclampsia among women who received vitamin D supplements was 3 % less than the control group. The NNT was 29 (95 % CI: 20 to 52).</p></div><div><h3>Conclusion</h3><p>Vitamin D supplementation significantly reduces preeclampsia during pregnancy.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102343"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139920601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acacia leucophloea (Roxb.). Willd.: Multi-targeted therapeutic efficacy against type 2 diabetes mellitus","authors":"Beena Levakumar Abhirami ,&nbsp;Anithakumari Aswathy Krishna ,&nbsp;Abdul Rahim Muhammed Jasim ,&nbsp;Alaganandam Kumaran","doi":"10.1016/j.eujim.2024.102344","DOIUrl":"https://doi.org/10.1016/j.eujim.2024.102344","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Acacia leucophloea</em> (AL) has been used traditionally to treat diabetes, cancer, inflammation, ophthalmia, haemorrhoids and leukoderma, however the scientific evidence validating its antidiabetic activity is limited. Employing a multi-targeted therapeutic strategy could be advantageous for the management of type 2 diabetes mellitus which is strongly correlated with oxidative stress-induced pathways<em>.</em> The present study was intended to evaluate the antidiabetic and antioxidant potential of AL via <em>in vitro</em> assays, validating its traditional medicinal significance.</p></div><div><h3>Methods</h3><p>Anti-diabetic potential of the AL extract, its fractions and isolated compounds was assessed through the determination of α-glucosidase, DPP-IV inhibition, anti-glycation activity, and glucose uptake using 2-NBDG in differentiated L6 myoblast, along with cytotoxicity studies. Total phenolic and flavonoid content was measured and the antioxidant potential was evaluated using <em>in vitro</em> assays <em>i.e.</em>, DPPH, ABTS and FRAP. In addition, quantitative determination of isolated compounds and their purity was analysed using HPLC-DAD.</p></div><div><h3>Results</h3><p>Fractions with the highest phenolic/flavonoid content (VI to XIII of AL) demonstrated substantial antioxidant and antidiabetic potential across selected therapeutic targets and these active fractions yielded six compounds upon fractionation using column chromatographic techniques. Structures of these compounds were examined using spectroscopic techniques and were identified as isoquercitrin, quercitrin, luteolin, epigallocatechin-3-O-(3<em>″</em>-O-methyl)-gallate, apigenin-7-O-glucoside and ethyl gallate. Isolated compounds showed significant DPP-IV inhibitory activity in a concentration-dependent manner with the highest activity with minimum IC₅₀ being possessed by isoquercitrin (IC₅₀ 4.28 ± 0.32 <em>µ</em>M). At a concentration of 200 <em>µ</em>M, apigenin-7-O-glucoside displayed a considerable glycation inhibitory effect (81.57 ± 2.66 %), surpassing the effect of other compounds. Moreover, at a concentration of 50 μM, the compounds apigenin-7-O-glucoside and isoquercitrin have increased the glucose uptake in L6 myotubes by 11.5 % and 11 % respectively, compared to a 6.1 % increase by the standard drug metformin. Notably, this study reveals for the first time the potential of both apigenin-7-O-glucoside and isoquercitrin to enhance glucose uptake in L6 myotubes.</p></div><div><h3>Conclusion</h3><p>AL and its isolated compounds exhibit promising effects in ameliorating post-prandial hyperglycemia. Their multifaceted therapeutic potential prompts further investigation to elucidate the underlying molecular mechanisms.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102344"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139985916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes for clinical research on Chinese medicine dampness syndrome: A protocol of developing a core outcome set","authors":"Xingying Qiu ,&nbsp;Jun Tan ,&nbsp;Wencong Cao ,&nbsp;Zehuai Wen ,&nbsp;Geng Li","doi":"10.1016/j.eujim.2023.102319","DOIUrl":"10.1016/j.eujim.2023.102319","url":null,"abstract":"<div><h3>Introduction</h3><p>Chinese medicine (CM)/integrative medicine have been widely used to treat dampness syndrome, regarded as the main pathogenesis of many chronic diseases. Many clinical trials of CM/integrative medicine for dampness syndrome have been conducted. However, most of their conclusions should be viewed with caution because of their methodological quality defects. One of the defects is the inconsistency of outcomes, which results in obstacles to comparing and combining the results of related studies. This issue could be addressed by developing a core outcome set (COS).</p></div><div><h3>Method</h3><p>This study aims to develop a COS for clinical research of CM dampness syndrome (COS-CMDS). We will set up a study advisory group to organize and guide the development of the COS. The study consists of four stages: (1) a systematic review of CM dampness syndrome; (2) qualitative interviews with experts and patients to construct a preliminary outcome pool based on the systematic review; (3) a two-round Delphi survey; and (4) a consensus meeting to determine the final COS-CMDS. This study has been approved by the institute review board and has been registered at the Core Outcome Measures in Effectiveness Trials Initiative (COMET) database (<span>https://www.comet-initiative.org/Studies/Details/1950</span><svg><path></path></svg>).</p></div><div><h3>Discussion</h3><p>The development of COS-CMDS will provide a reference for outcome selection in clinical research on CM dampness syndrome, and contribute to the combination and comparison of results reported during systematic reviews and meta-analyses. It may help generate more high-quality evidence.</p></div><div><h3>Conclusion</h3><p>COS-CMDS will standardize the selection and reporting of outcomes in clinical research of CM dampness syndrome. It will be published in appropriate ways to promote further widespread use after completion.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102319"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1876382023000951/pdfft?md5=ed3d337fa42c2ab933e2f26bca2034ef&pid=1-s2.0-S1876382023000951-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138508598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profile by LC-MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extracts","authors":"Esma Yeniçeri ,&nbsp;Ahmet Altay ,&nbsp;Ekrem Koksal ,&nbsp;Sevgi Altın ,&nbsp;Parham Taslimi ,&nbsp;Mustafa Abdullah Yılmaz ,&nbsp;Oğuz Cakir ,&nbsp;Abbas Tarhan ,&nbsp;Ali Kandemir","doi":"10.1016/j.eujim.2024.102337","DOIUrl":"10.1016/j.eujim.2024.102337","url":null,"abstract":"<div><h3>Introduction</h3><p>Studies on different species of <em>Onobrychis</em>, a member of the Fabaceae family, have revealed a number of biological activities with potential applications in alternative medicine. The phytochemical content of the plant <em>Onobrychis argyrea</em> subsp. <em>argyrea</em> and some of the important biological activities linked to its metabolism are quite limited. Therefore, this study was the first to determine the content of secondary metabolites in this species and to elucidate important biological activities related to metabolism.</p></div><div><h3>Methods</h3><p>LC-MS/MS was used for the quantitative analysis of bioactive compounds in the aerial part of <em>O. argyrea</em> extracts. The antioxidant properties of the extracts were assessed based on their ability to scavenge DPPH radicals and reduce iron ions. The anti-diabetic effect of the extracts was tested by the inhibition of α-amylase and α-glycosidase enzymes, and the anti-Alzheimer's ability was tested by the inhibition of the enzymes AChE and BChE. The cytotoxic effects of the extracts were tested on four different cancer lines, namely A-549, HT-29, MCF-7 and MDA-MB 453 using the XTT assay. The mechanism of the anti-cancer effect was determined by means of flow cytometry analysis on HT-29 cells.</p></div><div><h3>Results</h3><p>Twenty-nine phytochemicals were identified via the LC-MS/MS analysis in the plant extracts. The most abundant phytochemicals in the extracts were quinic acid, isoquercitrin, epicatechin, and routine, respectively. Antioxidant analysis showed that among all extracts, methanol extract (ME) was most effective in scavenging DPPH (IC₅₀: 23.93 ± 0.96 µg/mL) and in reducing iron ions (53.24 ± 2.14 mgTEAC/g). Enzyme activity studies demonstrated that ME is a potent inhibitor of α-glycosidase (IC₅₀: 15.06±0.64 µg/mL), AChE (IC₅₀: 24.75±0.76 µg/mL), and BChE (IC₅₀: 3.36±0.14 µg/mL). XTT assay results disclosed the strongest anti-proliferative activity of ME against the human colorectal adenocarcinoma HT-29 cell line with an IC₅₀ value of 33.35±0.92 µg/mL. Flow cytometry analysis revealed that ME caused mitochondrial membrane damage in HT-29 cells (**p&lt;0.01) and subsequently induced apoptosis (**p&lt;0.01) by activating the caspase pathway (**p&lt;0.001).</p></div><div><h3>Conclusion</h3><p><em>O. argyrea</em>, rich in bioactive compounds, showed remarkable antioxidant, antidiabetic, Alzheimer's, and anticancer properties <em>in vitro</em>, demonstrating that this plant contains secondary metabolites that may be potential drug candidates for the treatment of metabolic diseases.</p></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"66 ","pages":"Article 102337"},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139516499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}