European Journal of Integrative Medicine最新文献

{"title":"Metabolomic signature in ocular dosing: Exploring the metabolic impacts of sublethal high-dose naringenin on ARPE-19 cells","authors":"Ozan Kaplan ,&nbsp;Emine Koç ,&nbsp;Seyhan Türk ,&nbsp;Tuba Tüylü Küçükkılınç ,&nbsp;Zeynep Göktaş ,&nbsp;Mustafa Çelebier","doi":"10.1016/j.eujim.2024.102414","DOIUrl":"10.1016/j.eujim.2024.102414","url":null,"abstract":"<div><h3>Introduction</h3><div>Naringenin (NRG), a flavanone polyphenol found in citrus fruits, has been increasingly recognized for its potential therapeutic effects in ocular disorders. This study aimed to assess the impact of high-dose NRG on human retinal pigment epithelial cells (ARPE-19) cells through metabolomic analysis.</div></div><div><h3>Methods</h3><div>Cell viability was evaluated using the thiazolyl blue tetrazolium bromide (MTT) assay, demonstrating non-cytotoxicity at concentrations ranging from 3 to 100 μM within a 24-h exposure period. High-dose (100 μM) NRG was selected for further investigation based on its non-cytotoxic nature. Chromatographic analyses were performed using Liquid Chromatography Quadropole Time-of-Flight Mass Spectrometry (LC-Q-ToF-MS).</div></div><div><h3>Results</h3><div>Metabolomic analysis revealed subtle metabolic changes, with similar profiles between control and test groups, emphasizing the nuanced effects of NRG. Multivariate analysis, including Principal Component Analysis (PCA), illustrated distinct clustering of control and test groups, indicating consistent metabolic adjustments within each group. Despite maintaining cell viability, stress responses were identified in ARPE-19 cells, as evidenced by reduced glucose-6-phosphate levels and alterations in pyrimidine/purine pathways. This highlights the importance of exploring cellular responses using metabolomics beyond traditional viability metrics.</div></div><div><h3>Conclusion</h3><div>In the broader context, this study contributes data on the interplay between NRG and retinal pigmented epithelial. As NRG is utilized in routine ocular applications, particularly as an eye drop, our findings suggest careful dosage selection, considering both non-irritability and subtle metabolic changes. Further research is needed to refine dosage strategies and comprehensively assess the safety profile of NRG in ocular applications.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"72 ","pages":"Article 102414"},"PeriodicalIF":1.9,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of neuromodulatory effects of Origanum punonense danin essential oil on AMPA receptor function using whole-cell patch-clamp technique","authors":"Mohammad Qneibi ,&nbsp;Kamal Issa ,&nbsp;Amjad Bakhatan ,&nbsp;Majde Abu Khaled ,&nbsp;Sosana Bdir ,&nbsp;Mohammad Bdair ,&nbsp;Dana Sandouka ,&nbsp;Nidal Jaradat","doi":"10.1016/j.eujim.2024.102411","DOIUrl":"10.1016/j.eujim.2024.102411","url":null,"abstract":"<div><h3>Introduction</h3><div>: The quest for natural compounds with neuromodulatory properties has gained significant momentum in neuropharmacology. Among these, <em>Origanum punonense (O. punonense)</em> essential oil (EO) stands out due to its rich bioactive profile, particularly characterized by carvacrol. This study aimed to elucidate the effects of <em>O. punonense</em> EO on α-amino-3‑hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPAR), crucial synaptic plasticity, and cognitive function mediators.</div></div><div><h3>Methods</h3><div>: Leaves of <em>O. punonense</em> were collected along the Dead Sea coast in Jericho. After washing, it was dried in the shade for 15 days and ground. Essential oil was extracted using a microwave-ultrasonic method, with ultrasonic waves to enhance extraction. Using whole-cell patch-clamp techniques, we studied the effects of <em>O. punonense EO</em> on AMPAR kinetics in HEK293t cells transfected with homomeric and heteromeric subunits.</div></div><div><h3>Results</h3><div>: <em>O. punonense</em> EO significantly reduced AMPAR's whole-cell currents, indicating a targeted modulation of synaptic responses. Oil administration inhibited the currents for glutamate receptor subunits GluA1 (reduced from 885±58 pA to 342±20 pA), GluA1/2 (from 627±77 pA to 209±36 pA), GluA2 (from 1125±112 pA to 256±37 pA), and GluA2/3 (from 474±79 pA to 125±31 pA). The EO decreased the desensitization rate significantly for GluA2 and GluA2/3 (<em>p</em> &lt; 0.01) and for GluA1/2 (<em>p</em> &lt; 0.05), except for GluA1. In addition, the EO increased the deactivation rate significantly for GluA2 and GluA2/3 (<em>p</em> &lt; 0.01) and for GluA1/2 (<em>p</em> &lt; 0.05), with no effect observed on GluA1.</div></div><div><h3>Conclusions</h3><div>: This study highlights the possible properties of <em>O. punonense</em> EO and suggests future research to understand its medicinal benefits in neurodegenerative diseases.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"72 ","pages":"Article 102411"},"PeriodicalIF":1.9,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dose-effect relationship of acupuncture in the treatment of functional gastrointestinal disorders: A meta-regression of randomized controlled trials","authors":"Siyao Gong,&nbsp;Xin Yu,&nbsp;Jiao Yang,&nbsp;Dan Huang,&nbsp;Shuguang Yu,&nbsp;Sha Yang","doi":"10.1016/j.eujim.2024.102406","DOIUrl":"10.1016/j.eujim.2024.102406","url":null,"abstract":"<div><h3>Introduction</h3><div>The efficacy of acupuncture in treating functional gastrointestinal disorders (FGIDs) has been widely acknowledged. However, the dose-response relationship between acupuncture frequency and FGIDs remains uncertain. This review aimed to explore the relationship between the number of acupuncture sessions and the efficacy of acupuncture for FGIDs.</div></div><div><h3>Methods</h3><div>Seven major databases were searched from their inception to April 25, 2023. Literature screening, risk of bias assessment, and data extraction were performed by two reviewers independently. The quality of included randomized controlled trials (RCTs) was assessed using the Cochrane bias risk tool and Jadad score. The dose-effect relationship between acupuncture sessions and the clinical response rate in patients with FGIDs was investigated by using the cubic spline limited nonlinear meta-regression method in statal5.1. Before the review, we conducted the PROSPERO registration (CRD42022344699).</div></div><div><h3>Results</h3><div>Our analysis included 100 studies on acupuncture treatment of FGIDs, encompassing 8,589 participants. The meta-analysis of the dose-response relationship indicated that acupuncture could improve clinical symptoms and clinical response in patients with FGIDs to some extent. The clinical response rate increased with the number of acupuncture sessions in the range of ten to twenty sessions, with the odds ratio (OR) value rising from 1.05 (95 % CI: 0.89, 1.21) to 1.38 (95 % CI: 1.22,1.53). The peak of clinical response rate was observed at 24 acupuncture sessions (OR = 1.40; 95 % CI: 1.25, 1.55), representing a 40 % increase in OR. Compared with 24 sessions, the clinical response rate decreased after 56 sessions (OR = 1.22; 95 % CI: 0.64, 1.81).</div></div><div><h3>Conclusion</h3><div>There is a dose-response relationship between the number of acupuncture sessions and the clinical response in individuals with FGIDs. Twenty-four acupuncture treatments correlate with the optimal clinical response.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102406"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and anti-urease activities of Cardamine bulbifera: Insights from molecular docking and density functional theory studies","authors":"Emine İncilay Torunoğlu ,&nbsp;Erdi Can Aytar ,&nbsp;Betül Aydın ,&nbsp;Alper Durmaz","doi":"10.1016/j.eujim.2024.102410","DOIUrl":"10.1016/j.eujim.2024.102410","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Cardamine bulbifera</em> (L.) Crantz, commonly known as “Asian bittercress”, is a plant species widely distributed across Asia. It belongs to the Brassicaceae and has been traditionally used in medicinal applications. This study investigates the phytochemical composition, phenolic content, antioxidant properties, and anti-urease activity of <em>C. bulbifera.</em></div></div><div><h3>Methods</h3><div>In this study, the aerial parts of the <em>C. bulbifera</em> were collected. The total polyphenol content of the extracts was determined using spectrophotometric methods, and its antioxidant and anti-urease activities were assessed. The chemical composition was characterised using gas chromatography-mass spectroscopic (GC-MS) analysis. Additionally, molecular docking studies explored potential interactions between the identified compounds and <em>Helicobacter pylori</em> CagA oncoprotein. Furthermore, Density Functional Theory (DFT) analysis provided valuable insights into the electronic properties of the main compounds.</div></div><div><h3>Result</h3><div>The total phenolic content of <em>C. bulbifera</em> extract was 225 ± 0.02 mg GAE/g extract DW, and the total flavonoid content was 62.64 ± 3.27 mg QE/g extract DW. The 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay revealed an IC₅₀ value of 1.01 ± 0.04 mg/mL, indicating significant antioxidant activity. The IC₅₀ value for anti-urease activity was determined to be 2.74 ± 0.03 µg/mL, suggesting potent inhibition of urease enzyme activity. GC-MS analysis identified 15 bioactive compounds in the extract. Molecular docking studies highlighted Phenol, 3,5-bis(1,1-dimethylethyl) and 1-dodecanol as compounds with the highest binding affinity for the <em>H. pylori</em> CagA oncoprotein, suggesting potential therapeutic implications against <em>H. pylori</em>-related cancers. Additionally, DFT analysis emphasised these compounds' electronic properties and chemical reactivities, indicating their potential role in future pharmaceutical developments.</div></div><div><h3>Conclusion</h3><div><em>C. bulbifera</em> exhibits rich phenolic and flavonoid content and significant antioxidant and intense urease inhibition activities. These findings suggest that <em>C. bulbifera</em> may offer potential therapeutic options for conditions related to <em>H. pylori</em> infections, including cancer. Further research is needed to explore its mechanisms of action and clinical applications in more detail.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102410"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should eighteen incompatibilities be used? A systematic review and meta-analysis of the analgesic effect of pinellia and aconitum used externally for cancer pain","authors":"Can Cao ,&nbsp;Xiaoqing Liu ,&nbsp;Meijing Wu ,&nbsp;Xiangnan Xu ,&nbsp;Wenyong Liao ,&nbsp;Xiao Dong ,&nbsp;Yi Tian ,&nbsp;Ying Zhang ,&nbsp;Gansheng Zhong","doi":"10.1016/j.eujim.2024.102407","DOIUrl":"10.1016/j.eujim.2024.102407","url":null,"abstract":"<div><h3>Introduction</h3><div>Many clinical formulas containing the couplet Chinese medicines pinellia and aconitum (CMPA) have been recognized and demonstrated analgesic efficacy. However, CMPA belongs to the \"eighteen incompatibilities\" of traditional Chinese medicine theory, which is considered prohibited and dangerous by some medical practitioners. Whether CMPA can truly be used safely and without concern in the clinic urgently requires a more cohesive evaluation of the evidence. This review aims to evaluate the effectiveness and safety of CMPA for external use in the treatment of cancer pain.</div></div><div><h3>Methods</h3><div>We searched PubMed, Embase, Cochrane Library, CNKI, Wanfang, VIP, and Sinomed databases to collect randomized controlled trials (RCTs) comparing CMPA with conventional medicines for the treatment of cancer pain from their inception to December 31, 2023. Meta-analysis was conducted using RevMan 5.4 software. The modified Cochrane risk of bias tool was used to assess the quality of the included studies, and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) evidence profile was constructed to illustrate the certainty of evidence.</div></div><div><h3>Results</h3><div>A total of 1083 patients were enrolled in 14 RCTs. The results of the meta-analysis showed that the numerical rating scale (NRS) pain score [MD=-0.84, 95 % CI (-1.21, -0.47), <em>P</em> &lt; 0.00001, moderate certainty], duration of continuous analgesia [MD=1.45 h, 95 % CI (1.13, 1.77), <em>P</em> &lt; 0.00001, low certainty], and Karnofsky performance status (KPS) quality of life score [MD=7.25, 95 % CI (5.01, 9.48), <em>P</em> &lt; 0.00001, low certainty] of patients after external administration of CMPA-containing prescriptions in combination with conventional medicine therapy were better than those with the conventional medicine therapy. The incidence of total adverse reactions [RR=0.69, 95 % CI (0.58, 0.83), <em>P</em> &lt; 0.0001, moderate certainty] and constipation [RR=0.43, 95 % CI (0.19, 0.97), <em>P</em> = 0.04, moderate certainty] of patients after external administration of CMPA-containing prescriptions in combination with conventional medicine therapy were lower than those with the conventional medicine therapy. No statistically significant differences were found regarding the effectiveness or safety of CMPA-containing prescriptions compared to conventional medicine therapy (low or very low certainty evidence).</div></div><div><h3>Conclusion</h3><div>The external use of CMPA-containing prescriptions, when combined with conventional medicine therapy, is safer and more effective for cancer pain than conventional medicine therapy. However, higher-level evidence is still warranted.</div></div><div><h3>Review registration</h3><div>PROSPERO registration number: CRD42023400792.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102407"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142415980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pueraria lobata radix (Ge Gen)-containing traditional Chinese medicine prescriptions for the treatment of nonalcoholic fatty liver disease: A systematic review and meta-analysis","authors":"Haoyu Wang ,&nbsp;Huilin Liu ,&nbsp;Xiangmin Deng ,&nbsp;Ping Luo ,&nbsp;Yaqi Tian ,&nbsp;Rui Zhang ,&nbsp;Gaochuan Zhang ,&nbsp;Xunxun Yuan ,&nbsp;Xu Zhou","doi":"10.1016/j.eujim.2024.102405","DOIUrl":"10.1016/j.eujim.2024.102405","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to systematically evaluate the effectiveness and safety of <em>Pueraria lobata radix</em> (Chinese name: Ge Gen)-containing prescriptions (GGPs) for the treatment of nonalcoholic fatty liver disease (NAFLD).</div></div><div><h3>Methods</h3><div>Seven databases were searched for randomized controlled trials (RCTs) comparing the effectiveness of GGPs plus basic therapies (Western medicines or lifestyle interventions) versus the same basic therapies in patients with NAFLD. The primary outcomes were liver function indicators, and the secondary outcomes included metabolism indicators, severity of symptoms, and incidence of adverse events. The effects were measured as weighted mean differences (WMDs), standard mean differences (SMDs), and 95 % confidence intervals (CIs).</div></div><div><h3>Results</h3><div>Eighteen RCTs involving 1,991 patients were included. Meta-analysis results showed that compared to the control group, the GGP group had a significant improvement in liver function (aspartate aminotransferase: WMD -9.60 U/L, 95 % CI -14.96 to -4.25; alanine aminotransferase:6.30 U/L, 95 % CI -9.57 to -3.02; gamma-glutamyl transferase:6.35 U/L, 95 % CI -10.44 to -2.25), blood lipid levels (total cholesterol: WMD -0.68 mmol/L, 95 % CI -0.88 to -0.49; triglycerides:0.61 mmol/L, 95 % CI -0.84 to -0.38; low-density lipoprotein cholesterol:0.42 mmol/L, 95 % CI -0.56 to -0.27; high-density lipoprotein cholesterol: 0.34 mmol/L, 95 % CI 0.21 to 0.47), body mass index (WMD -0.79 kg/m², 95 % CI -1.38 to -0.19), blood glucose levels (fasting blood glucose: WMD -1.03 mmol/L, 95 % CI -1.52 to -0.44; 2-hour postprandial blood glucose:1.06 mmol/L, 95 % CI -1.68 to -0.28; glycosylated hemoglobin:0.56 %, 95 % CI -0.85 to -0.28; homeostatic model assessment of insulin resistance:0.85 mmol/L, 95 % CI -1.15 to -0.56; fasting insulin:2.34 mmol/L, 95 % CI -3.14 to -1.55), and symptom severity scores (SMD -1.91, 95 % CI -2.48 to -1.34). The GGP group reported six mild adverse events, including diarrhea, nausea, and stomach discomfort.</div></div><div><h3>Conclusion</h3><div>GGPs, as an adjunct therapy for NAFLD, may improve liver function, metabolic parameters, and clinical symptoms, with good safety. However, the quality of evidence was moderate-to-very low owing to the presence of a moderate-to-high risk of bias and significant heterogeneity.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102405"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142415979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DOTTs-Database of Therapeutic Plants against Tuberculosis: Rationalizing traditional medicine for therapeutic applications","authors":"Hazarika Risha ,&nbsp;Gangar Tarun ,&nbsp;Patra Sanjukta","doi":"10.1016/j.eujim.2024.102409","DOIUrl":"10.1016/j.eujim.2024.102409","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Mycobacterium tuberculosis,</em> a highly resistant superbug causing tuberculosis disease (TB), contributes to one of the leading causes of death worldwide. Improved drug regimens and therapeutics have been extensively studied, but still none are proven to be fully effective in eliminating the pathogen or its drug-resistant forms. Therefore, alternative and novel therapeutic approaches to address this problem are needed. Traditional medicine can be vital in managing TB via codified (Ayurveda, Siddha, Unani) and uncodified systems (folk medicine). In recent years, the utilization of medicinal plants for TB therapy has garnered considerable attention. Identifying underexplored plants utilized as a remedy for TB can aid in developing future treatment strategies. The current study aims to assemble the ethnobotanical literature that provides insight into the undocumented tribal/folk medicinal practices against tuberculosis in one unified platform.</div></div><div><h3>Methods</h3><div>The medicinal plants with therapeutic properties against tuberculosis were identified manually by screening published literature. The data is represented to the users using a user-friendly web interface developed via the PHP-HTML platform using Bootstrap Cascading Style Sheet (CSS) theme, and the database is managed by MySQL Database Management System (DBMS).</div></div><div><h3>Results</h3><div>Database of Therapeutic Plants against Tuberculosis (DOTTs) describes the medicinal plants used traditionally in different forms against tuberculosis by various indigenous tribes and communities in India. A total of 100 plants with their local names, taxonomical classification (order and family), tribe, plant part, and formulations, as well as the anti-tubercular attributes, are provided in the database. Additional information about the plants can be retrieved from the external links provided.</div></div><div><h3>Conclusion</h3><div>The DOTTs database provides the ethnobotanical and ethnopharmacological data of 100 Indian medicinal plants. This database will help the researchers acquire information about the plants that could be beneficial for further evaluation and discovery of potential leads in TB drug discovery. With proper investigation and advancement, it is anticipated that TB could no longer be a serious public health risk.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102409"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of music interventions on anxiety, pain, and pregnancy rates in women undergoing fertility treatment before oocyte collection and embryo transfer: Systematic review and meta-analysis of randomized controlled trials","authors":"Mehtap Kızılkaya ,&nbsp;Aysu Yıldız Karaahmet ,&nbsp;Kerime Derya Beydağ","doi":"10.1016/j.eujim.2024.102408","DOIUrl":"10.1016/j.eujim.2024.102408","url":null,"abstract":"<div><h3>Introdution</h3><div>The present review aimed to evaluate the available evidence in the literature on the effect of music interventions on improving outcomes related to anxiety, pain, and pregnancy rates of women undergoing fertility treatment, prior to oocyte collection and embryo transfer.</div></div><div><h3>Method</h3><div>We searched the following databases from database inception to 2 May 2024, PUBMED, Embase, Web of Science, and the Turkish Academic Network and Information Center (ULAKBIM). Randomized controlled trials (RCT) in Turkish and English languages were included in the screening. Articles were scanned using MeSH-based keywords. The data were analyzed using the Review Manager computer program (Version 5.4.1).</div></div><div><h3>Results</h3><div>A total of 8 RCTs, including 1634 female patients, were included in our review. Music interventions significantly reduced anxiety scores prior to oocyte collection or fertilization compared to the control group (Standardised Mean Difference (SMD):0.26, 95 % CI:0.39 to 0.13, <em>Z</em> = 3.91, <em>p</em> &lt; 0.0001). Moreover, after the music treatment, there was a significant improvement in the pain score during the procedure (SMD:0.96, 95 % CI:1.72 to -0.20, <em>Z</em> = 2.49, <em>p</em> = 0.01). Although more women in the music interventions group experienced an increase in the clinical pregnancy rate compared to the control group, there was no evidence of an effect on pregnancy rate (SMD:0.14, 95 % CI:2.63 to 2.34, <em>Z</em> = 0.11, <em>p</em> = 0.91).</div></div><div><h3>Conclusion</h3><div>Music interventions appears to improve anxiety and pain scores in female patients receiving fertility treatment when administered during oocyte collection and fertilization. They may increase the clinical pregnancy rate but this not statistically significant. More research with larger sample sizes is needed to investigate the impact of music interventions on assisted reproductive technology clinical outcomes.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"72 ","pages":"Article 102408"},"PeriodicalIF":1.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yiqi Huoxue Tongluo formula-containing serum attenuates high glucose-induced injury in microglia via MAPK pathways: Network pharmacology-based analysis and biological validation","authors":"Chang Chang ,&nbsp;Qian Chen ,&nbsp;Ke Zuo ,&nbsp;Huilun Yuan ,&nbsp;Lujie Wang ,&nbsp;Aijuan Jiang ,&nbsp;Yuqing Wang","doi":"10.1016/j.eujim.2024.102403","DOIUrl":"10.1016/j.eujim.2024.102403","url":null,"abstract":"<div><h3>Introduction</h3><div>The Yiqi Huoxue Tongluo formula (YQHX), containing Astragalus mongholicus Bunge, Angelica sinensis (Oliv.) Diels, Rehmannia glutinosa Libosch., Pueraria lobata (Willd.) Ohwi, Corydalis yanhusuo W.T.Wang, Spatholobus suberectus Dunn, Clematis chinensis Osbeck, is a proprietary formula of the First Affiliated Hospital of Anhui University of Chinese Medicine, and can attenuate diabetic neuropathy pain (DNP). This study aimed to explore the mechanisms underlying YQHX-mediated DNP alleviation. Additionally, the effects of YQHX on BV2 microglia injured by high glucose via the mitogen-activated protein kinase (MAPK) signaling pathways were investigated.</div></div><div><h3>Methods</h3><div>Liquid chromatography tandem mass spectroscopy (LC-MS/MS) was performed to identify the chemical compounds in YQHX. The potential mechanism of YQHX for treating DNP was explored through network pharmacology analysis. The established glucose-induced BV2 cell injury model was treated with varying concentrations of YQHX-containing serum. The effects of YQHX-containing serum and MAPK pathway inhibitors on microglial cells were assessed by Cell Counting Kit-8 and enzyme-linked immunosorbent assays. The messenger RNA (mRNA) and protein levels of microglial marker OX42 were measured. Additionally, phosphorylated-p38, -c-Jun-terminal kinase, and -extracellular signal-regulated kinase and downstream nuclear factor-κB/p65 and phosphorylated-activating transcription factor 2 (ATF2) proteins were detected by western blotting.</div></div><div><h3>Results</h3><div>In total, 503 chemical components were identified in YQHX by LC-MS/MS analysis. Network pharmacology results showed 203 active ingredients and 363 overlapping targets, respectively, as potential therapeutic candidates for YQHX-mediated DNP treatment. Additionally, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis yielded a total of 105 signal pathways. Notably, YQHX considerably improved the cell viability of BV2 cells (<em>P</em> &lt; 0.01), remarkably reduced the production of proinflammatory factors (<em>P</em> &lt; 0.01), significantly increased the production of anti-inflammatory factors (<em>P</em> &lt; 0.01), and markedly inhibited the mRNA and protein levels of OX42 (<em>P</em> &lt; 0.01). Furthermore, YQHX treatment inhibited MAPK activation and ATF2 expression.</div></div><div><h3>Conclusions</h3><div>YQHX may protect against high-glucose-induced BV2 microglial cell injury by inhibiting the activation of MAPKs and downstream ATF2, thus, reducing microglial activation and proinflammatory cytokine release. These results provide a foundational theoretical basis for exploring the further mechanism of YQHX in treating DNP, and they provide a novel target for the application of traditional Chinese medicine.</div></div>","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102403"},"PeriodicalIF":1.9,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Adverse events associated with the use of cannabis-based products in people living with cancer: A scoping review protocol” [European Journal of Integrative Medicine, Volume 62, 102279, 2023]","authors":"Irene Cheah ,&nbsp;Ingrid Gelissen ,&nbsp;Jennifer Hunter ,&nbsp;Joanna Harnett","doi":"10.1016/j.eujim.2024.102402","DOIUrl":"10.1016/j.eujim.2024.102402","url":null,"abstract":"","PeriodicalId":11932,"journal":{"name":"European Journal of Integrative Medicine","volume":"71 ","pages":"Article 102402"},"PeriodicalIF":1.9,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1876382024000714/pdfft?md5=39daadce02743dfdf35a63b3b956547d&pid=1-s2.0-S1876382024000714-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}