Enzyme & protein最新文献_第3页

Cooperation between matrix metalloproteinases and the plasminogen activator-plasmin system in tumor progression. 基质金属蛋白酶与纤溶酶原激活物-纤溶酶系统在肿瘤进展中的协同作用。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468617

Y A DeClerck, W E Laug

引用次数: 96

Urokinase plasminogen activator as a predictor of aggressive disease in breast cancer. 尿激酶纤溶酶原激活剂作为乳腺癌侵袭性疾病的预测因子

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468618

M J Duffy, C Duggan, T Maguire, K Mulcahy, P Elvin, E McDermott, J J Fennelly, N O'Higgins

引用次数: 36

Angiogenesis: a paradigm for balanced extracellular proteolysis during cell migration and morphogenesis. 血管生成:在细胞迁移和形态发生过程中平衡的细胞外蛋白水解的范例。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468622

M S Pepper, R Montesano, S J Mandriota, L Orci, J D Vassalli

{"title":"Angiogenesis: a paradigm for balanced extracellular proteolysis during cell migration and morphogenesis.","authors":"M S Pepper, R Montesano, S J Mandriota, L Orci, J D Vassalli","doi":"10.1159/000468622","DOIUrl":"https://doi.org/10.1159/000468622","url":null,"abstract":"Extracellular proteolysis is required for matrix degradation and the regulation of cytokine activity during angiogenesis, and this is dependent on a cohort of proteases and protease inhibitors produced by endothelial and nonendothelial cells. The plasminogen activator (PA)/plasmin system has been extensively investigated in these processes, and descriptive studies have demonstrated that urokinase-type PA (uPA), uPA receptor (uPAR) and PA inhibitor-1 (PAI-1) are expressed by endothelial cells during angiogenesis in vivo. In vitro studies have led to the notion that normal capillary morphogenesis is dependent on a protease-antiprotease equilibrium. These findings are discussed in the context of recent observations on uPA-, uPAR-, PAI-1 and plaminogen-deficient mice, in which developmental and physiological angiogenesis appear to occur normally. This has led to a reevaluation of the role of the PA/plasmin system during angiogenesis. In particular, these observations raise the possibility that the role of this system may be limited to situations in which endothelial cells encounter and must degrade fibrin in order to form new capillary sprouts.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468622","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19768265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 225

The integrity of thiol groups is essential for catalytic efficiency of rat liver cholesterol ester hydrolase either in microsomal membranes or after solubilization. 巯基的完整性对大鼠肝脏胆固醇酯水解酶在微粒体膜或溶解后的催化效率至关重要。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468638

M López de Heredia, S Cristóbal, M L Hernández, M J Martínez, B Ochoa

{"title":"The integrity of thiol groups is essential for catalytic efficiency of rat liver cholesterol ester hydrolase either in microsomal membranes or after solubilization.","authors":"M López de Heredia, S Cristóbal, M L Hernández, M J Martínez, B Ochoa","doi":"10.1159/000468638","DOIUrl":"https://doi.org/10.1159/000468638","url":null,"abstract":"Neutral cholesterol ester hydrolase from rat liver microsomes was inactivated in a dose and time-dependent manner by classical sulphydryl-reacting reagents such as p-hydroxymercuribenzoic acid, 5,5'-dithio-bis-(2-nitrobenzoic acid), N-ethylmaleimide, or iodoacetate. The concentrations at which half-maximal inhibition of the native microsomal cholesterol ester hydrolase occurred (IC50) were 15, 68, and 370 mumol/l and 68 mmol/l, respectively. Only partial reactivation of the enzyme was observed under excess dithiothreitol or mercaptoethanol treatment. The stimulation of cholesterol ester hydrolase by the metal ions Ca2+ and Mg2+ was dependent on the integrity of the thiol groups. Solubilization of cholesterol ester hydrolase from membranes preserved its sensitivity towards sulphydryl reagents and thiols, as well as its ability to be activated by Ca2+ and Mg2+. Dithiothreitol, mercaptoethanol, and Ca2+ and Mg2+ provided total protection of the enzyme against inactivation by thiol-reacting reagents. The results indicate that one or more thiol groups are either at the active centre of the native and solubilized forms of rat liver microsomal cholesterol ester hydrolase or are sufficiently near, to interfere with the catalysis when they are reacted.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20196919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The role of stromelysin-1 in stromal-epithelial interactions and cancer. 基质溶素-1在基质-上皮相互作用和癌症中的作用。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468624

J F Wiesen, Z Werb

引用次数: 22

Proteasomes. Multicatalytic proteinase complexes. 水解酶。多催化蛋白酶复合物。

Enzyme & protein Pub Date : 1994-12-13 DOI: 10.1159/ISBN.978-3-318-06186-4

S. Wilk

引用次数: 4

Kinetics of irreversible inhibition of yeast alcohol dehydrogenase during modification by o-phthaldehyde. 邻苯二醛修饰酵母醇脱氢酶的不可逆抑制动力学。

Enzyme & protein Pub Date : 1994-01-01 DOI: 10.1159/000474985

W P Le, S X Yan, M Q Huang, Y X Zhang, H M Zhou

引用次数: 4

Yeast porphobilinogen deaminase also forms enzyme-pyrrole intermediates. 酵母卟啉原脱氨酶也形成酶-吡咯中间体。

Enzyme & protein Pub Date : 1994-01-01 DOI: 10.1159/000475000

S Correa Garcia, M V Rossetti, M Bermudez Moretti, A M Batlle

引用次数: 1

Pig platelet acidic carboxypeptidases. 猪血小板酸性羧肽酶。

Enzyme & protein Pub Date : 1994-01-01 DOI: 10.1159/000475002

H Ostrowska

引用次数: 3

Diagnostic significance of urinary enzymes for diabetes mellitus and hypertension. 尿酶对糖尿病和高血压的诊断意义。

Enzyme & protein Pub Date : 1994-01-01 DOI: 10.1159/000474984

N Ishii, Z Ogawa, H Itoh, H Ikenaga, T Saruta

{"title":"Diagnostic significance of urinary enzymes for diabetes mellitus and hypertension.","authors":"N Ishii, Z Ogawa, H Itoh, H Ikenaga, T Saruta","doi":"10.1159/000474984","DOIUrl":"https://doi.org/10.1159/000474984","url":null,"abstract":"In order to evaluate tubular damage in diabetic patients, the activity of renal proximal tubule derived enzymes excreted in 24-hour urine were recorded in 5 groups as follows: (i) 48 noninsulin-independent diabetic patients with normal renal function and a urinary albumin excretion rate within the normal range; (ii) 45 noninsulin-dependent diabetic patients with normal renal function and a high urinary albumin level; (iii) 26 noninsulin-dependent diabetic patients with renal failure; (iv) 40 patients with essential hypertension and normal renal function, and (v) 48 normal control subjects. Regardless of whether cases were noninsulin-dependent diabetics with normal or high urinary albumin excretion rate or cases with renal dysfunction, urinary dipeptidyl aminopeptidase IV and N-acetyl-beta-D-glucosaminidase excretions were significantly higher than in healthy subjects, and urinary gamma-glutamyl transpeptidase excretion was significantly lower than in healthy subjects. No significant changes in urinary enzyme excretions showed specific variations in the essential hypertensive patients. These results suggest that there is tubular damage in the early stages of noninsulin-dependent diabetic patients with normal renal function and normal urinary albumin excretion rate. Detection of urinary excretion of dipeptidyl aminopeptidase IV, N-acetyl-beta-D-glucosaminidase and gamma-glutamyl transpeptidase may be especially useful for the early diagnosis of diabetic nephropathy.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000474984","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19569778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20