Enzyme & protein最新文献_第2页

Cathepsin B: multiple enzyme forms from a single gene and their relation to cancer. 组织蛋白酶B:来自单一基因的多种酶形式及其与癌症的关系。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468619

D Keppler, B F Sloane

引用次数: 76

Alterations and role of human cathepsin D in cancer metastasis. 人组织蛋白酶D在肿瘤转移中的改变及其作用。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468620

H Rochefort, E Liaudet, M Garcia

引用次数: 46

Combined 3-methylglutaconic and 3-hydroxy-3-methylglutaric aciduria with endocardial fibroelastosis and dilatative cardiomyopathy in male and female siblings with partial deficiency of complex II/III in fibroblasts. 合并3-甲基戊二酸和3-羟基-3-甲基戊二酸尿伴心内膜纤维弹性增生和扩张性心肌病在成纤维细胞复合体II/III部分缺乏的男性和女性兄弟姐妹中

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468642

S Ruesch, S Krähenbühl, S Kleinle, S Liechti-Gallati, T Schaffner, B Wermuth, J Weber, U N Wiesmann

{"title":"Combined 3-methylglutaconic and 3-hydroxy-3-methylglutaric aciduria with endocardial fibroelastosis and dilatative cardiomyopathy in male and female siblings with partial deficiency of complex II/III in fibroblasts.","authors":"S Ruesch, S Krähenbühl, S Kleinle, S Liechti-Gallati, T Schaffner, B Wermuth, J Weber, U N Wiesmann","doi":"10.1159/000468642","DOIUrl":"https://doi.org/10.1159/000468642","url":null,"abstract":"We report on 2 children, brother and sister, who presented with cardiomyopathy and muscular hypotonia at the age of B months. They both excreted significant amounts of 3-hydroxy-3-methylglutaric acid (3-HMG) and 3-methylglutaconic acid (3-MGC) but no 3-methylglutaric acid (3-MG). Enzyme analysis in fibroblasts revealed normal activities of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase and of 3-methylglutaconyl hydratase and other enzymes of 3-HMG metabolism. Loading tests with leucine did not affect the excretion of 3-HMG and 3-MGC. The girl died as a result of her cardiomyopathy, while the boy recovered and was treated with cardiac supportive therapy. He showed a steady improvement during his clinical course with biochemical normalization of the urinary excretion of 3-HMG, concomitant with marked improvement in the hypertrophic cardiomyopathy. In cultured fibroblasts from both patients a reduced activity of complex II/III of the respiratory chain was measured which may be the cause of this new type of 3-HMG uria. Analysis of mitochondrial DNA heart muscle, liver and fibroblast culture of the patient did not reveal any major mitochondrial DNA rearrangements (deletion, duplication) or any point mutation that had been described in association with mitochondrial cardiomyopathy.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468642","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20196923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Proteases associated with invadopodia, and their role in degradation of extracellular matrix. 与侵殖相关的蛋白酶及其在细胞外基质降解中的作用。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468616

W T Chen

引用次数: 131

A variant alkaline phosphatase detected in a patient with lung cancer. 在肺癌患者中检测到一种变异碱性磷酸酶。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468641

M Miyanaga, H Sugimoto, T Komoda, O Nosjean, K Honma, K Nemoto, T Sato

引用次数: 14

Mechanisms controlling the transcription of matrix metalloproteinase genes in normal and neoplastic cells. 基质金属蛋白酶基因在正常和肿瘤细胞中的转录调控机制。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468614

H C Crawford, L M Matrisian

{"title":"Mechanisms controlling the transcription of matrix metalloproteinase genes in normal and neoplastic cells.","authors":"H C Crawford, L M Matrisian","doi":"10.1159/000468614","DOIUrl":"https://doi.org/10.1159/000468614","url":null,"abstract":"Matrix metalloproteinases (MMPs) are expressed in normal remodeling tissues in a generally tissue-restricted pattern. Transcripts for stromelysin-1 and collagenase are expressed primarily in stromal fibroblasts, whereas transcripts for matrilysin are expressed primarily in glandular epithelial cells. These expression patterns are maintained at carcinoma tumor sites until the late stages of tumor progression at which point many epithelially-derived tumors begin to express stromal fibroblast MMPs. Coincidentally, late stage carcinomas take on other characteristics of stromal fibroblasts, indicating that these tumor cells have \"transdifferentiated', that is, they have begun to exhibit characteristics of cells from a separate developmental lineage. Despite their distinct expression patterns, many of the promoters for MMP genes show the same general arrangement of the nuclear proto-oncoprotein-binding sites, AP-1 and PEA3. However, the specific interaction between these cis-elements and different combinations of Fos, Jun, and Ets proteins which recognize these sites may be important in controlling both the positive and negative regulation involved in the tissue-restricted pattern of MMP expression in normal and neoplastic tissues.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19766943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 200

Stromal cell expression of components of matrix-degrading protease systems in human cancer. 基质降解蛋白酶系统成分在人类癌症中的基质细胞表达。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468623

R Hewitt, K Danø

引用次数: 80

Synthesis of guanidinosuccinate from argininosuccinate and reactive oxygen in vitro. 精氨酸琥珀酸盐与活性氧体外合成胍基琥珀酸盐。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468629

K Aoyagi, S Nagase, C Tomida, K Takemura, K Akiyama, A Koyama

{"title":"Synthesis of guanidinosuccinate from argininosuccinate and reactive oxygen in vitro.","authors":"K Aoyagi, S Nagase, C Tomida, K Takemura, K Akiyama, A Koyama","doi":"10.1159/000468629","DOIUrl":"https://doi.org/10.1159/000468629","url":null,"abstract":"Synthesis of guanidinosuccinic acid (GSA), a uremic toxin, has been suggested to relate to the urea concentration and synthetic rate. Among the urea cycle enzymes, inhibition of argininosuccinate (ASA) lyase by urea has been reported. Argininosuccinate which contains a GSA structure is a candidate of a GSA precursor. We found that another uremic toxin, methylguanidine, is formed from creatinine with reactive oxygen species. Therefore, we investigated in vitro whether GSA is formed from ASA with reactive oxygen species. GSA was measured by HPLC by a post-column-labeling method using 9,10-phenathrequinone. When 1 mmol/l ASA was reacted with the hydroxyl radical-generating system for 5 min at pH 7.4, 9 mumol/l GSA was formed. Dimethylsulfoxide, a hydroxyl radical scavenger, markedly inhibited GSA synthesis. The superoxide radical generated by xanthine and xanthine oxidase reaction also formed 1 mumol/l GSA from 1 mumol/l ASA and the GSA formation was inhibited by superoxide dismutase or catalase almost completely. Addition of FeCl2 to the xanthine/xanthine oxidase reaction further increased GSA synthesis. These results indicate that GSA is formed from ASA by reaction with the hydroxyl radical and the superoxide radical.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19988182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Micromethod for the fluorimetric determination of plasma N-acetyl-alpha-D-galactosaminidase and study of some of its characteristics. 荧光显微法测定血浆n -乙酰- α - d -半乳糖胺酶及其一些特性的研究。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468637

W R Den Tandt, S Scharpé

{"title":"Micromethod for the fluorimetric determination of plasma N-acetyl-alpha-D-galactosaminidase and study of some of its characteristics.","authors":"W R Den Tandt, S Scharpé","doi":"10.1159/000468637","DOIUrl":"https://doi.org/10.1159/000468637","url":null,"abstract":"We have studied some characteristics of N-acetyl-alpha-D-galactosaminidase in human plasma using a sensitive and very simple fluorimetric (single tube incubation/fixation) micromethod. The enzyme has a pH optimum at 4.5, is linear on incubation during at least 6 h, is protected from inactivation at room temperature by acidification, and is stable on freezing (about 85% residual activity after 1 year at -20 degrees C). Enzyme kinetics indicate that the affinity for the substrate is low (K(m) value 7 mmol/l). By studying about 10 possible effectors, no activation was found by detergents. As expected, the colorigenic p-nitrophenyl derivative substrate, N-acetylgalactosamine and galactose are low-affinity inhibitors. A histogram of 108 control samples showed a unimodal distribution pattern with a slight bias to the right. No pseudodeficients were found on analysis of 220 control plasma samples. Patients with alpha-galactosaminidosis had residual activity between 0.7 and 2.1%. In patients with 17 different lysosomal storage diseases, no increase was found except in mucolipidosis II and III. The main advantages of the method are its simplicity sensitivity, short incubation time requirement and economy in substrate consumption. The method can be used either for screening or diagnostic purposes of genetic N-acetyl-alpha-D-galactosaminidase deficiency.","PeriodicalId":11854,"journal":{"name":"Enzyme & protein","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468637","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20195754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Kinetic studies of the effects of K+, Na+ and Li+ on the catalytic activity of human erythrocyte pyridoxal kinase. K+、Na+和Li+对人红细胞吡哆醛激酶催化活性影响的动力学研究。

Enzyme & protein Pub Date : 1996-01-01 DOI: 10.1159/000468639

P Lainé-Cessac, P Allain

引用次数: 15