M Miyanaga, H Sugimoto, T Komoda, O Nosjean, K Honma, K Nemoto, T Sato
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引用次数: 14
Abstract
A variant alkaline phosphatase (ALP), with heat-sensitivity characteristics similar to that of the bone type, was found in the serum of a patient suffering from lung cancer. In disc polyacrylamide gel electrophoretic studies most of this enzyme had migrated to the region corresponding to liver ALP, with the remainder affecting bone ALP. Like kidney ALP, this ALP was markedly inhibited by 0.5 mmol/l L-cysteine. The K(m) of this ALP for p-nitrophenylphosphate was 0.39 mmol/l, similar to that of kidney ALP. The sugar moiety of this enzyme bore greater resemblance to that of kidney ALP than liver or bone ALP. However, immunoprecipitation of this particular ALP was strong with a monoclonal antibody against liver ALP and moderate with an antibody against bone ALP.
在一名肺癌患者的血清中发现了一种碱性磷酸酶(ALP)的变体，它具有与骨型相似的热敏特性。在圆盘聚丙烯酰胺凝胶电泳研究中，大多数这种酶已经迁移到肝脏ALP对应的区域，其余的影响骨ALP。与肾ALP一样，0.5 mmol/l l -半胱氨酸显著抑制该ALP。对硝基苯基磷酸ALP的K(m)为0.39 mmol/l，与肾ALP相似。这种酶的糖部分与肾ALP比与肝或骨ALP更相似。然而，这种特异性ALP的免疫沉淀与抗肝ALP的单克隆抗体有关，而与抗骨ALP的抗体有关。
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