European cells & materials最新文献_第3页

Histology of tendon and enthesis - suitable techniques for specific research questions. 肌腱和椎体的组织学-适合特定研究问题的技术。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-05-24 DOI: 10.22203/eCM.v043a16

N. Angrisani, E. Willbold, A. Kampmann, A. Derksen, J. Reifenrath

{"title":"Histology of tendon and enthesis - suitable techniques for specific research questions.","authors":"N. Angrisani, E. Willbold, A. Kampmann, A. Derksen, J. Reifenrath","doi":"10.22203/eCM.v043a16","DOIUrl":"https://doi.org/10.22203/eCM.v043a16","url":null,"abstract":"The musculoskeletal system consists of different components comprising a wide range of tissue types, with tendons being one part. Tendon degeneration or rupture have a high prevalence in all age groups, often with poor outcomes of surgical treatment such as chronic pain and high re-tear rates. Therefore, much effort has been directed to further develop diagnostic and therapeutic methods as well as reconstruction techniques, including using adequate placeholders or implants. Diagnostic approaches and advanced stages of preclinical studies will inevitably include histological examination of the pathologically affected tissue. The present study presents adequate tendon-related, histological techniques, including the embedding of soft- and hard-tissue samples in different media. Consideration is also given to samples containing residual implant materials or having been subjected to standard staining protocols and immunohistochemical procedures. The study further examines cells and tendon structure to detect degenerative, fibrotic or inflammatory conditions and possible foreign-body responses to implanted materials. Infraspinatus tendons from preclinical studies carried on rat and sheep samples, as well as human biceps tendon samples, have been used as example materials.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"380 ","pages":"228-251"},"PeriodicalIF":3.1,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41309600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Making connections: using anatomy to guide tissue engineering approaches at the enthesis. 建立联系：利用解剖学来指导附着点的组织工程方法。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-05-05 DOI: 10.22203/eCM.v043a14

C. Loukopoulou, JW Mortimer, JZ Paxton

{"title":"Making connections: using anatomy to guide tissue engineering approaches at the enthesis.","authors":"C. Loukopoulou, JW Mortimer, JZ Paxton","doi":"10.22203/eCM.v043a14","DOIUrl":"https://doi.org/10.22203/eCM.v043a14","url":null,"abstract":"The enthesis demonstrates a distinct highly ordered zonal microanatomy at the osteotendinous/osteoligamentous tissue connection that allows for the smooth transmission of mechanical forces between tissues. Interfacial tissue engineering (ITE), a subset of the interdisciplinary field of tissue engineering, is directed at replicating this complex transitional anatomy of the enthesis in vitro. Yet, the limited understanding of tissue boundaries, gradients and structural relationships at specific anatomical locations hampers the development of novel therapeutic strategies for bespoke enthesis regeneration, thus reducing their direct clinical applicability. This review provides an overview of ITE approaches for repair of the osteotendinous/osteoligamentous junction and highlights the importance of complementary inclusion of direct anatomical research. The cross-disciplinary collaboration across an array of experts, including anatomists, involved in the design, development and utilisation of bioengineered tissues will enhance the properties of such tissues and improve their clinical relevance. More specifically, a detailed anatomical analysis of the region of interest should drive the in vitro design and enable researchers to develop anatomically and clinically relevant tissue-engineered replacement tissues for human implantation. Finally, the present review discusses the challenges and future directions of the ITE field and highlights the importance of anatomically driven tissue engineering as an emerging tool in clinical translational research.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":" ","pages":"162-178"},"PeriodicalIF":3.1,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48866127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanostructured fibrin-based hydrogel membranes for use as an augmentation strategy in Achilles tendon surgical repair in rats. 纳米结构纤维蛋白基水凝胶膜在大鼠跟腱外科修复中用作增强策略。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-04-28 DOI: 10.22203/eCM.v043a13

D. González-Quevedo, D. Sánchez-Porras, Ó. García-García, J. Chato-Astrain, M. Díaz-Ramos, A. Campos, V. Carriel, F. Campos

{"title":"Nanostructured fibrin-based hydrogel membranes for use as an augmentation strategy in Achilles tendon surgical repair in rats.","authors":"D. González-Quevedo, D. Sánchez-Porras, Ó. García-García, J. Chato-Astrain, M. Díaz-Ramos, A. Campos, V. Carriel, F. Campos","doi":"10.22203/eCM.v043a13","DOIUrl":"https://doi.org/10.22203/eCM.v043a13","url":null,"abstract":"Hydrogels are polymeric biomaterials characterised by their promising biological and biomechanical properties, which make them potential alternatives for use in tendon repair. The aim of the present study was to generate in vitro, and determine the therapeutic efficacy in vivo, of novel nanostructured fibrin-based hydrogels to be used as an augmentation strategy for the surgical repair of rat Achilles tendon injuries. Fibrin, fibrin-agarose and fibrin-collagen nanostructured hydrogels (NFH, NFAH and NFCH, respectively) were generated and their biomechanical properties and cell-biomaterial interactions characterised ex vivo. Achilles tendon ruptures were created in 24 adult Wistar rats, which were next treated with direct repair (control group) or direct repair augmented with the generated biomaterials (6 rats/group). After 4 and 8 weeks, the animals were euthanised for macroscopical and histological analyses. Biomechanical characterisation showed optimal properties of the biomaterials for use in tendon repair. Moreover, biological analyses confirmed that tendon-derived fibroblasts were able to adhere to the surface of the generated biomaterials, with high levels of viability and functionality. In vivo studies demonstrated successful tendon repair in all groups. Lastly, histological analyses disclosed better tissue and extracellular matrix organisation and alignment with biomaterial-based augmentation strategies than direct repair, especially when NFAH and NFCH were used. The present study demonstrated that nanostructured fibrin-collagen hydrogels can be used to enhance the healing process in the surgical repair of tendon ruptures.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"26 8","pages":"162-178"},"PeriodicalIF":3.1,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41258946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MECHANICAL AND STRAIN BEHAVIOUR OF HUMAN ACHILLES TENDON DURING IN VITRO TESTING TO FAILURE 人跟腱在体外失效试验中的力学和应变行为

IF 3.1 3区医学

European cells & materials Pub Date : 2022-04-21 DOI: 10.22203/eCM.v043a12

C. Nagelli, A. Hooke, N. Quirk, C. L. de Padilla, T. Hewett, M. van Griensven, M. Coenen, L. Berglund, C. Evans, S. Müller

{"title":"MECHANICAL AND STRAIN BEHAVIOUR OF HUMAN ACHILLES TENDON DURING IN VITRO TESTING TO FAILURE","authors":"C. Nagelli, A. Hooke, N. Quirk, C. L. de Padilla, T. Hewett, M. van Griensven, M. Coenen, L. Berglund, C. Evans, S. Müller","doi":"10.22203/eCM.v043a12","DOIUrl":"https://doi.org/10.22203/eCM.v043a12","url":null,"abstract":"The Achilles tendon is the strongest tendon in the human body but its mechanical behaviour during failure has been little studied and the basis of its high tensile strength has not been elucidated in detail. In the present study, healthy, human, Achilles tendons were loaded to failure in an anatomically authentic fashion while the local deformation and strains were studied in real time, with very high precision, using digital image correlation (DIC). The values determined for the strength of the Achilles tendon were at the high end of those reported in the literature, consistent with the absence of a pre-existing tendinopathy in the samples, as determined by careful gross inspection and histology. Early in the loading cycle, the proximal region of the tendon accumulated high lateral strains while longitudinal strains remained low. However, immediately before rupture, the mid-substance of the Achilles tendon, its weakest part, started to show high longitudinal strains. These new insights advance the understanding of the mechanical behaviour of tendons as they are stretched to failure.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 1","pages":"153 - 161"},"PeriodicalIF":3.1,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44302132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

REGULATORS OF COLLAGEN CROSSLINKING IN DEVELOPING AND ADULT TENDONS 发育中和成年肌腱中胶原交联的调节因子

IF 3.1 3区医学

European cells & materials Pub Date : 2022-04-05 DOI: 10.22203/eCM.v043a11

A.J. Ellingson, N. M. Pancheri, N. Schiele

{"title":"REGULATORS OF COLLAGEN CROSSLINKING IN DEVELOPING AND ADULT TENDONS","authors":"A.J. Ellingson, N. M. Pancheri, N. Schiele","doi":"10.22203/eCM.v043a11","DOIUrl":"https://doi.org/10.22203/eCM.v043a11","url":null,"abstract":"Tendons are collagen-rich musculoskeletal tissues that possess the mechanical strength needed to transfer forces between muscles and bones. The mechanical development and function of tendons are impacted by collagen crosslinks. However, there is a limited understanding of how collagen crosslinking is regulated in tendon during development and aging. Therefore, the objective of the present review was to highlight potential regulators of enzymatic and non-enzymatic collagen crosslinking and how they impact tendon function. The main collagen crosslinking enzymes include lysyl oxidase (LOX) and the lysyl oxidase-like isoforms (LOXL), whereas non-enzymatic crosslinking is mainly mediated by the formation of advanced glycation end products (AGEs). Regulators of the LOX and LOXL enzymes may include mechanical stimuli, mechanotransducive cell signaling pathways, sex hormones, transforming growth factor (TGF)β family, hypoxia, and interactions with intracellular or extracellular proteins. AGE accumulation in tendon is due to diabetic conditions and aging, and can be mediated by diet and mechanical stimuli. The formation of these enzymatic and non-enzymatic collagen crosslinks plays a major role in tendon biomechanics and in the mechanisms of force transfer. A more complete understanding of how enzymatic and non-enzymatic collagen crosslinking is regulated in tendon will better inform tissue engineering and regenerative therapies aimed at restoring the mechanical function of damaged tendons.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 1","pages":"130 - 152"},"PeriodicalIF":3.1,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43904079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Controversies in regenerative medicine: should knee joint osteoarthritis be treated with mesenchymal stromal cells? 再生医学中的争议：膝关节骨性关节炎应该用间充质基质细胞治疗吗？

IF 3.1 3区医学

European cells & materials Pub Date : 2022-03-17 DOI: 10.22203/eCM.v043a09

R. Ossendorff, S. Walter, F. Schildberg, M. Khoury, G. Salzmann

{"title":"Controversies in regenerative medicine: should knee joint osteoarthritis be treated with mesenchymal stromal cells?","authors":"R. Ossendorff, S. Walter, F. Schildberg, M. Khoury, G. Salzmann","doi":"10.22203/eCM.v043a09","DOIUrl":"https://doi.org/10.22203/eCM.v043a09","url":null,"abstract":"Knee joint osteoarthritis is a complex immunological and degenerative disease. Current treatment strategies fail to alter its progression. Mesenchymal stromal cell (MSC) therapy for osteoarthritis has been object of research for more than 30 years. The aim of MSC therapy is intended to be holistic, with regeneration of all affected knee joint structures. The paracrine effect of the MSC secretome has been shown to be central for the regenerative capacity of MSCs. Activation of local knee-joint-specific MSCs leads to an immunomodulatory, anti-catabolic, anti-apoptotic and chondrogenic stimulus. Preclinical models have demonstrated the symptom- and disease-modifying effects of MSC therapy. At the bedside, there is evidence that autologous and allogeneic MSC therapy shows significant improvement in symptom-modifying and functional outcome. Despite this, a variety of contradictory clinical outcomes are available in the literature. The effectiveness of MSC therapy is still unclear, although there have been promising results. Regarding the diversity of cell sources, isolation, culture protocols and other factors, a comparison of different studies is difficult. Clinical translation of disease-modifying effects has not yet been shown. This narrative review presents a controversial overview of the current preclinical and clinical studies on MSC therapy in knee joint osteoarthritis.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 1","pages":"98-111"},"PeriodicalIF":3.1,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48509714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Characterisation of subchondral bone repair following transplantation of bioreactor-manufactured autologous osteochondral graft in a sheep model. 羊模型生物反应器制备的自体骨软骨移植后软骨下骨修复的特征。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-03-02 DOI: 10.22203/eCM.v043a08

P. Kostešić, A. Vukasović Barišić, I. Erjavec, M. Pušić, D. Hudetz, D. Matičić, D. Vnuk, M. Vučković, A. Ivković

{"title":"Characterisation of subchondral bone repair following transplantation of bioreactor-manufactured autologous osteochondral graft in a sheep model.","authors":"P. Kostešić, A. Vukasović Barišić, I. Erjavec, M. Pušić, D. Hudetz, D. Matičić, D. Vnuk, M. Vučković, A. Ivković","doi":"10.22203/eCM.v043a08","DOIUrl":"https://doi.org/10.22203/eCM.v043a08","url":null,"abstract":"To date, no single approach to the treatment of osteochondral defects has resulted in satisfactory long-term outcomes, especially in a young and active human population. Emerging innovative tissue engineering strategies, including the use of composite scaffolds, novel cell sources and bioreactors, have shown promising results. However, these techniques need to be validated in translational animal models before they can be implemented in clinical practice. The aim of the present study was to analyse morphological and microarchitectural parameters during subchondral bone repair following transplantation of bioreactor-manufactured autologous osteochondral grafts in a sheep model. Animals were divided into 4 treatment groups: nasal chondrocyte (NC) autologous osteochondral grafts, articular chondrocyte (AC) autologous osteochondral grafts, cell-free scaffolds (CFS) and empty defects (EDs). After 6 weeks, 3 months and 12 months, bone remodelling was assessed by histology and micro-computed tomography (µCT). Although gradual remodelling and subchondral bone repair were seen in all groups across the time points, the best results were observed in the NC group. This was evidenced by the extent of new tissue formation and its best integration into the surrounding tissue in the NC group at all time points. This also suggested that nasal septum chondrocyte-seeded grafts adapted well to the biomechanical conditions of the loaded joint surface.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 1","pages":"79-97"},"PeriodicalIF":3.1,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45200985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innate and adaptive immune system cells implicated in tendon healing and disease. 与肌腱愈合和疾病有关的先天性和适应性免疫系统细胞。

IF 3.2 3区医学

European cells & materials Pub Date : 2022-02-18 DOI: 10.22203/eCM.v043a05

G Crosio, A H Huang

引用次数: 0

In vitro investigations of Staphylococcus aureus biofilms in physiological fluids suggest that current antibiotic delivery systems may be limited. 对生理液体中金黄色葡萄球菌生物膜的体外研究表明，目前的抗生素输送系统可能存在局限性。

IF 3.2 3区医学

European cells & materials Pub Date : 2022-02-02 DOI: 10.22203/eCM.v043a03

S Isguven, K Fitzgerald, L J Delaney, M Harwood, T P Schaer, N J Hickok

{"title":"In vitro investigations of Staphylococcus aureus biofilms in physiological fluids suggest that current antibiotic delivery systems may be limited.","authors":"S Isguven, K Fitzgerald, L J Delaney, M Harwood, T P Schaer, N J Hickok","doi":"10.22203/eCM.v043a03","DOIUrl":"10.22203/eCM.v043a03","url":null,"abstract":"<p><p>Orthopaedic surgical site infections, especially when a hardware is involved, are associated with biofilm formation. Clinical strategies for biofilm eradication still fall short. The present study used a novel animal model of long-bone fixation with vancomycin- or gentamicin-controlled release and measured the levels of antibiotic achieved at the site of release and in the surrounding tissue. Then, using fluids that contain serum proteins (synovial fluid or diluted serum), the levels of vancomycin or gentamicin required to substantially reduce colonising bacteria were measured in a model representative of either prophylaxis or established biofilms. In the in vivo model, while the levels immediately adjacent to the antibiotic release system were up to 50× the minimal inhibitory concentration in the first 24 h, they rapidly dropped. At peripheral sites, values never reached these levels. In the in vitro experiments, Staphylococcus aureus biofilms formed in serum or in synovial fluid showed a 5-10 fold increase in antibiotic tolerance. Importantly, concentrations required were much higher than those achieved in the local delivery systems. Finally, the study determined that the staged addition of vancomycin and gentamicin was not more efficacious than simultaneous vancomycin and gentamicin administration when using planktonic bacteria. On the other hand, for biofilms, the staged addition seemed more efficacious than adding the antibiotics simultaneously. Overall, data showed that the antibiotics' concentrations near the implant in the animal model fall short of the concentrations required to eradicate biofilms formed in either synovial fluid or serum.</p>","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 ","pages":"6-21"},"PeriodicalIF":3.2,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9199942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial - Brothers in arms: regenerative biology and dentistry. 社论-战友:再生生物学和牙科。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-01-03 DOI: 10.22203/eCM.v043a02

T A Mitsiadis, O Trubiani

引用次数: 1